Posts Tagged ‘Recombinant DNA’

The late Cambridge Mayor Alfred Vellucci welcomed Life Sciences Labs to Cambridge, MA – June 1976

Reporter: Aviva Lev-Ari, PhD, RN

How Cambridge became the Life Sciences Capital

Worth watching is the video below, which captures the initial Cambridge City Council hearing on recombinant DNA research from June 1976. The first speaker is the late Cambridge mayor Alfred Vellucci.

Vellucci hoped to pass a two-year moratorium on gene splicing in Cambridge. Instead, the council passed a three-month moratorium, and created a board of nine Cambridge citizens — including a nun and a nurse — to explore whether the work should be allowed, and if so, what safeguards would be necessary. A few days after the board was created, the pro and con tables showed up at the Kendall Square marketplace.

At the time, says Phillip Sharp, an MIT professor, Cambridge felt like a manufacturing town that had seen better days. He recalls being surrounded by candy, textile, and leather factories. Sharp hosted the citizens review committee at MIT, explaining what the research scientists there planned to do. “I think we built a relationship,” he says.

By early 1977, the citizens committee had proposed a framework to ensure that any DNA-related experiments were done under fairly stringent safety controls, and Cambridge became the first city in the world to regulate research using genetic material.



How Cambridge became the life sciences capital

Scott Kirsner can be reached at kirsner@pobox.com. Follow him on Twitter@ScottKirsner and on betaboston.com.


How Cambridge became the life sciences capital


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Transphosphorylation of E-coli Proteins and Kinase Specificity

Reporter: Larry H Bernstein, MD, FCAP


Transphosphorylation of E. coli proteins during production of recombinant protein kinases provides a robust system to characterize kinase specificity

X Wu, Man-Ho Oh, HS Kim, D Schwartz,… Huber SC.

Front. Plant Sci. 3:262.    http://dx.doi.org/ 10.3389/fpls.2012.00262 

Four lines of evidence suggest that transphosphorylation of E. coli proteins by BRI1

is specific and therefore provides meaningful results:

(1) phosphorylation is

(2) phosphosite stoichiometry, estimated by spectral counting, is also

  •  not related to protein abundance;

(3) a transphosphorylation motif emerged

  •  with strong preference for basic residues both N- and C-terminal to the phosphosites; and

(4) other protein kinases (BAK1, PEPR1, FLS2, and CDPKβ) phosphorylated a distinct set of E. coli proteins and phosphosites. http://fpls.com/Transphosphorylation_of_E._coli_proteins_during_production_of_recombinant_protein_kinases_provides_a_robust_system_to_characterize_kinase_specificity/

English: Structure of the BAK1 protein. Based ...

English: Structure of the BAK1 protein. Based on PyMOL rendering of PDB 2ims. (Photo credit: Wikipedia)

Escherichia coli - Scanning Electron Microscopy

Escherichia coli – Scanning Electron Microscopy (Photo credit: Wikipedia)

English: Protein kinase CK2, a phosphorylation...

English: Protein kinase CK2, a phosphorylation enzyme (Photo credit: Wikipedia)

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