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Transphosphorylation of E-coli Proteins and Kinase Specificity

Reporter: Larry H Bernstein, MD, FCAP

https://pharmaceuticalintelligence.com/2013/03/04/transphosphory…i-ptoteins-and/

Transphosphorylation of E. coli proteins during production of recombinant protein kinases provides a robust system to characterize kinase specificity

X Wu, Man-Ho Oh, HS Kim, D Schwartz,… Huber SC.

Front. Plant Sci. 3:262.    http://dx.doi.org/ 10.3389/fpls.2012.00262 

Four lines of evidence suggest that transphosphorylation of E. coli proteins by BRI1

is specific and therefore provides meaningful results:

(1) phosphorylation is

(2) phosphosite stoichiometry, estimated by spectral counting, is also

  •  not related to protein abundance;

(3) a transphosphorylation motif emerged

  •  with strong preference for basic residues both N- and C-terminal to the phosphosites; and

(4) other protein kinases (BAK1, PEPR1, FLS2, and CDPKβ) phosphorylated a distinct set of E. coli proteins and phosphosites. http://fpls.com/Transphosphorylation_of_E._coli_proteins_during_production_of_recombinant_protein_kinases_provides_a_robust_system_to_characterize_kinase_specificity/

English: Structure of the BAK1 protein. Based ...

English: Structure of the BAK1 protein. Based on PyMOL rendering of PDB 2ims. (Photo credit: Wikipedia)

Escherichia coli - Scanning Electron Microscopy

Escherichia coli – Scanning Electron Microscopy (Photo credit: Wikipedia)

English: Protein kinase CK2, a phosphorylation...

English: Protein kinase CK2, a phosphorylation enzyme (Photo credit: Wikipedia)

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