Transphosphorylation of E-coli Proteins and Kinase Specificity

March 4, 2013 by larryhbern

Transphosphorylation of E-coli Proteins and Kinase Specificity

Reporter: Larry H Bernstein, MD, FCAP

https://pharmaceuticalintelligence.com/2013/03/04/transphosphory…i-ptoteins-and/

Transphosphorylation of E. coli proteins during production of recombinant protein kinases provides a robust system to characterize kinase specificity

X Wu, Man-Ho Oh, HS Kim, D Schwartz,… Huber SC.

Front. Plant Sci. 3:262. http://dx.doi.org/ 10.3389/fpls.2012.00262

Four lines of evidence suggest that transphosphorylation of E. coli proteins by BRI1

is specific and therefore provides meaningful results:

(1) phosphorylation is

not correlated with bacterial protein abundance;

(2) phosphosite stoichiometry, estimated by spectral counting, is also

not related to protein abundance;

(3) a transphosphorylation motif emerged

with strong preference for basic residues both N- and C-terminal to the phosphosites; and

(4) other protein kinases (BAK1, PEPR1, FLS2, and CDPKβ) phosphorylated a distinct set of E. coli proteins and phosphosites. http://fpls.com/Transphosphorylation_of_E._coli_proteins_during_production_of_recombinant_protein_kinases_provides_a_robust_system_to_characterize_kinase_specificity/

English: Structure of the BAK1 protein. Based on PyMOL rendering of PDB 2ims. (Photo credit: Wikipedia)

Escherichia coli – Scanning Electron Microscopy (Photo credit: Wikipedia)

English: Protein kinase CK2, a phosphorylation enzyme (Photo credit: Wikipedia)

The floppy protein: When being out of shape finally pays off (ns.umich.edu)
Over Active Enzyme Discovered In Failing Hearts (medicalnewstoday.com)
Phosphorylation and Feedback Regulation of Metabotropic Glutamate Receptor 1 by Calcium/Calmodulin-Dependent Protein Kinase II (jneurosci.org)
Cloning the vaccinia virus genome as a bacterial artificial chromosome (pharmaceuticalintelligence.com)
Recombinant Production of Human Interleukin 6 in Escherichia coli (plosone.org)
Scien.net Publishes New Kinase and Oxidase Bibliography (prweb.com)
Native Tertiary Structure and Nucleoside Modifications Suppress tRNA’s Intrinsic Ability to Activate the Innate Immune Sensor PKR (plosone.org)
“Magnetogenetics” probes the inner space of a cell (arstechnica.com)

4 Responses

on March 5, 2013 at 4:30 AM | Reply sonsondaimari

Reblogged this on SYNCHEMHOT.
on March 6, 2013 at 3:29 AM | Reply Transphosphorylation of E-coli proteins and kinase specificity … | Neuro Physiology Blog

[…] Read More: Transphosphorylation of E-coli proteins and kinase specificity … […]
on July 24, 2014 at 12:26 PM | Reply Series B: Frontiers in Genomics Research | Scientific Curation Services

[…] 5.9 Transphosphorylation of E-coli Proteins and Kinase Specificity […]
- on July 24, 2014 at 4:44 PM | Reply larryhbern
  
  *Transphosphorylation *
  
  also important for PTM
  
  On Thu, Jul 24, 2014 at 1:29 PM, Pharmaceutical Intelligence wrote:
  
  >

Comments RSS

Leaders in Pharmaceutical Business Intelligence (LPBI) Group

Funding, Deals & Partnerships: BIOLOGICS & MEDICAL DEVICES; BioMed e-Series; Medicine and Life Sciences Scientific Journal – http://PharmaceuticalIntelligence.com