Transphosphorylation of E-coli Proteins and Kinase Specificity
Reporter: Larry H Bernstein, MD, FCAP
http://pharmaceuticalintelligence.com/2013/03/04/transphosphory…i-ptoteins-and/
X Wu, Man-Ho Oh, HS Kim, D Schwartz,… Huber SC.
Front. Plant Sci. 3:262. http://dx.doi.org/ 10.3389/fpls.2012.00262
Four lines of evidence suggest that transphosphorylation of E. coli proteins by BRI1
is specific and therefore provides meaningful results:
(1) phosphorylation is
- not correlated with bacterial protein abundance;
(2) phosphosite stoichiometry, estimated by spectral counting, is also
- not related to protein abundance;
(3) a transphosphorylation motif emerged
- with strong preference for basic residues both N- and C-terminal to the phosphosites; and
(4) other protein kinases (BAK1, PEPR1, FLS2, and CDPKβ) phosphorylated a distinct set of E. coli proteins and phosphosites. http://fpls.com/Transphosphorylation_of_E._coli_proteins_during_production_of_recombinant_protein_kinases_provides_a_robust_system_to_characterize_kinase_specificity/
English: Structure of the BAK1 protein. Based on PyMOL rendering of PDB 2ims. (Photo credit: Wikipedia)
Escherichia coli – Scanning Electron Microscopy (Photo credit: Wikipedia)
English: Protein kinase CK2, a phosphorylation enzyme (Photo credit: Wikipedia)
Related articles
- The floppy protein: When being out of shape finally pays off (ns.umich.edu)
- Over Active Enzyme Discovered In Failing Hearts (medicalnewstoday.com)
- Phosphorylation and Feedback Regulation of Metabotropic Glutamate Receptor 1 by Calcium/Calmodulin-Dependent Protein Kinase II (jneurosci.org)
- Cloning the vaccinia virus genome as a bacterial artificial chromosome (pharmaceuticalintelligence.com)
- Recombinant Production of Human Interleukin 6 in Escherichia coli (plosone.org)
- Scien.net Publishes New Kinase and Oxidase Bibliography (prweb.com)
- Native Tertiary Structure and Nucleoside Modifications Suppress tRNA’s Intrinsic Ability to Activate the Innate Immune Sensor PKR (plosone.org)
- “Magnetogenetics” probes the inner space of a cell (arstechnica.com)
2012pharmaceutical
Amandeep Kaur
Aashir Awan, Phd
Abhisar Anand
Adina Hazan
Alan F. Kaul, PharmD., MS, MBA, FCCP
alexcrystal6
anamikasarkar
apreconasia
aptubman
Arav Gandhi
aviralvatsa
David Orchard-Webb, PhD
danutdaagmailcom
Demet Sag, Ph.D., CRA, GCP
Dror Nir
dsmolyar
Ethan Coomber
evelinacohn
FrasonKalapurackal
Gail S Thornton
Irina Robu
jayzmit48
jdpmd
jshertok
kellyperlman
Ed Kislauskis
larryhbern
Madison Davis
marzankhan
megbaker58
ofermar2020
Dr. Pati
pkandala
ritusaxena
Rick Mandahl
sjwilliamspa
Srinivas Sriram
stuartlpbi
Dr. Sudipta Saha
tildabarliya
zraviv06
zs22
I actually consider this amazing blog , âSAME SCIENTIFIC IMPACT: Scientific Publishing –
Open Journals vs. Subscription-based « Pharmaceutical Intelligenceâ, very compelling plus the blog post ended up being a good read.
Many thanks,Annette
I actually consider this amazing blog , âSAME SCIENTIFIC IMPACT: Scientific Publishing –
Open Journals vs. Subscription-based « Pharmaceutical Intelligenceâ, very compelling plus the blog post ended up being a good read.
Many thanks,Annette