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Posts Tagged ‘Predictive Cancer Biomarkers’


5:00 – 5:45 PM Early Diagnosis Through Predictive Biomarkers, NonInvasive Testing

Reporter: Stephen J. Williams, Ph.D.

 

Diagnosing cancer early is often the difference between survival and death. Hear from experts regarding the new and emerging technologies that form the next generation of cancer diagnostics.

Moderator: Heather Rose, Director of Licensing, Thomas Jefferson University
Speakers:
Bonnie Anderson, Chairman and CEO, Veracyte @BonnieAndDx
Kevin Hrusovsky, Founder and Chairman, Powering Precision Health @KevinHrusovsky

Bonnie Anderson and Veracyte produces genomic tests for thyroid and other cancer diagnosis.  Kevin Hrusovksy and Precision Health uses peer reviewed evidence based medicine to affect precision medicine decision.

Bonnie: aim to get a truth of diagnosis.  Getting tumor tissue is paramount as well as properly preserved tissue.  They use deep RNA sequencing  and machine learning  in their clinically approved tests.

Kevin: Serial biospace entrepreneur.  Two diseases, cancer and neurologic, have been diseases which have been hardest to get reproducible and validated biomarkers of early disease.  He concentrates on protein biomarkers.

Heather:  FDA has recently approved drugs for early disease intervention.  However the use of biomarkers can go beyond patient stratification in clinical trials.

Kevin: 15 approved drugs for MS but the markers are scans looking for brain atrophy which is too late of an endpoint.  So we need biomarkers of early disease progression.  We can use those early biomarkers of disease progression so pharma can target those early biomarkers and or use those early biomarkers of disease progression  for endpoint

Bonnie: exciting time in the early diagnostics field. She prefers transcriptomics to DNA based methods such as WES or WGS (whole exome or whole genome sequencing).  It was critical to show data on the cost savings imparted by their transcriptomic based thryoid cancer diagnostic test for payers to consider this test eligible for reimbursement.

Kevin: There has been 20 million  CAT scans for  cancer but it is estimated 90% of these scans led to misdiagnosis. Biomarker  development  has revolutionized diagnostics in this disease area.  They have developed a breakthrough panel of ten protein biomarkers in serum which he estimates may replace 5 million mammograms.

All panelists agreed on the importance of regulatory compliance and the focus of new research should be on early detection.  In addition they believe that Dr. Gotlieb’s appointment to the FDA is a positive for the biomarker development field, as Dr. Gotlieb understands the potential and importance of early detection and prevention of disease.  Kevin also felt Dr. Gotlieb understands the importance of incorporating biomarkers as endpoints in clinical trials.  Over 750 phase 1,2, and 3 clinical trials use biomarker endpoints but the pharma companies still need to prove the biomarkers clinical relevance to the FDA.They also agreed it would be helpful to involve advocacy groups in putting more pressure on the healthcare providers and policy makers on this importance of diagnostics as a preventative measure.

In addition, the discovery and use of biomarkers as disease endpoints has led to a resurgence of Alzheimer’s disease drug development by companies which have previously given up on these type of neurodegenerative diseases.

Kevin feels proteomics offers great advantages over DNA-based diagnostics, especially in cancer such as ovarian cancer, where a high degree of specificity for a diagnostic test is required to ascertain if a woman should undergo prophylactic oophorectomy.  He suggests that a new blood-based protein biomarker panel is being developed for early detection of some forms of ovarian cancer.

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Please see related articles on Live Coverage of Previous Meetings on this Open Access Journal

LIVE – Real Time – 16th Annual Cancer Research Symposium, Koch Institute, Friday, June 16, 9AM – 5PM, Kresge Auditorium, MIT

Real Time Coverage and eProceedings of Presentations on 11/16 – 11/17, 2016, The 12th Annual Personalized Medicine Conference, HARVARD MEDICAL SCHOOL, Joseph B. Martin Conference Center, 77 Avenue Louis Pasteur, Boston

Tweets Impression Analytics, Re-Tweets, Tweets and Likes by @AVIVA1950 and @pharma_BI for 2018 BioIT, Boston, 5/15 – 5/17, 2018

BIO 2018! June 4-7, 2018 at Boston Convention & Exhibition Center

https://pharmaceuticalintelligence.com/press-coverage/

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Cambridge Healthtech Institute’s Ninth Annual Predictive Cancer Biomarkers, August 26, 2016 Symposia, Washington DC

Reporter: Aviva Lev-Ari, PhD, RN

 

 

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Predictive cancer biomarkers hold the promise of early cancer detection, personalized treatment, and accurate patient monitoring. Cambridge Healthtech Institute’s Ninth Annual Predictive Cancer Biomarkers symposium will focus on current research regarding tumor pathways and biomarker discovery, the progress toward clinical applications such as early detection and therapy monitoring, and case studies focusing on clinical utility and actionability, how many patients benefit from these assays, the role of genetic counselors, and practicality of funding and reimbursement. FDA validation will also be discussed. Join top cancer researchers and your peers to examine discoveries, best practices, and future directions.

RECOMMENDED PRE-CONFERENCE SHORT COURSES*

SC3: Translating CTCs for Clinical Use

SC14: Liquid Biopsy: Clinical Applications and Business Considerations

*Separate registration required

FRIDAY, AUGUST 26

8:00 am Registration & Morning Coffee

IDENTIFYING BIOMARKERS AND PATHWAYS

8:25 Chairperson’s Opening Remarks

Anuradha Murali, Ph.D., MSCR, Clinical Trial Manager, Gastroenterology Associates of Orangeburg

8:30 Integrated Genomic Analysis of Pancreatic Ductal Adenocarcinomas Reveals Genomic Rearrangement Events as Significant Drivers of Disease

George_VasmatzisGeorge Vasmatzis, Ph.D., Director, Biomarker Discovery Program, Center for Individualized Medicine, Mayo Clinic

In this study, we performed mate pair sequencing (MPseq) on genomic DNA from 24 PDAC tumors, including 15 laser-captured microdissected PDAC and 9 patient-derived xenografts, to identify genome-wide rearrangements. Large genomic rearrangements with intragenic breakpoints altering key regulatory genes involved in PDAC progression were detected in all tumors. SMAD4, ZNF521, and FHIT were among the most frequently hit genes. Conversely, commonly reported genes with copy number gains, including MYC and GATA6, were frequently observed in the absence of direct intragenic breakpoints, suggesting a requirement for sustaining oncogenic function during PDAC progression. Additionally, a number of potentially targetable amplifications and fusions have been observed opening the possibility to genomically driven targetable treatment in PDAC.

9:00 Towards Cancer Diagnostics Based on Interphase Spatial Genome Positioning

Karen_MeaburnKaren Meaburn, Ph.D., Research Scientist, NCI, NIH

The genome is non-randomly organized in the cell nucleus. We have identified several genes that robustly alter position in breast or prostate cancer. These genes, used singularly or in combination, are able to distinguish cancer from normal tissue with high accuracy. We are working towards exploiting these changes in positioning patterns to develop a novel diagnostic strategy for the detection of cancer.

9:30 FEATURED PRESENTATION: Ultraconserved Elements: The Enigma and the Potential

C.-Ting_WuC.-Ting Wu, Ph.D., Professor, Genetics, Harvard Medical School

Ultraconserved elements (UCEs) are DNA sequences that have been perfectly (100%) conserved for 300-500 million years. Because neither protein coding, nor enhancer, nor transcription factor binding, nor promoter regions require such conservation, the mere existence of UCEs has been a long debated conundrum. We propose that UCEs contribute to genome integrity and, hence, may provide a strategy by which otherwise healthy tissues can be culled of cells harboring deleterious rearrangements.

10:00 A Novel rRNA Depletion Method to Enable Whole Transcriptome Analysis of Single Cells with RNA-Seq

Maureen Peterson, Ph.D., Product Development Scientist, Research & Development, NuGEN Technologies

RNA-Seq analyses of transcripts from large numbers of cells often masks biologically relevant differences that occur in individual cells. Understanding RNA expression in a single cell has great potential for biomarker development, and monitoring disease progression and therapy. We describe a method for generating RNA-Seq libraries from single cells that enables removal of specific transcripts without perturbing the original total RNA population, providing a solution for biomarker discovery and diagnostic applications using extremely limited samples.

10:30 Coffee Break

MOVING TOWARDS CLINICAL APPLICATIONS

11:00 Circulating Cell-Free DNA, RNA and Exosomes –Key Biomarkers for Early Cancer Detection and LiquidBiopsy Diagnostics

Michael_HellerMichael J. Heller, Ph.D., Professor, Bioengineering & Nanoengineering, University of California San Diego

Exosomes and their associated RNA and proteins, as well as circulating cell-free DNA are now considered to be important key biomarkers for cancer diagnostics ranging from early detection to liquid biopsy for patient management and therapy monitoring. Nevertheless, the isolation and detection of these biomarkers, exosomes in particular, require considerable time and effort which greatly limits their practical, reliable and more widespread use in cancer diagnostics. Using a DEP device, we are now able to isolate all of these key biomarkers, including exosomes, in 15-20 minutes from 25-50ul of blood, plasma or serum. In the case of glioblastoma exosomes isolated from plasma, specific surface and interior proteins CD63 and TSG101 could then be detected by immunofluorescence, and EGFRvlll mutations in mRNA were detected by RT-PCR.

11:30 Using Molecular Profiling in Early Clinical Trials

Barbara_ConleyBarbara Conley, M.D., Associate Director, Cancer Diagnosis Program, Division of Cancer Treatment & Diagnosis, National Cancer Institute (NCI)

The talk will inform the audience of the thinking behind the design of the NCI-MATCH trial, which profiles a fresh biopsy of patients with malignancies who have progressed on standard treatment. This trial uses a validated locked platform of NGS, supplemented by IHC assays, to assign patients to treatment arms based on molecular eligibility criteria, regardless of histology. The trial accrued very robustly from first opening. The MATCH trial is a signal-finding trial based on levels of evidence for both the treatments and the molecular eligibility criteria. Lessons learned will be presented. As such it represents a type of clinical validation of potentially predictive molecular features, and presents the opportunity to learn about the effect of co-mutations on response rate.

12:00 pm Tumor Heterogeneity and the Course of Mutation Detection Following Treatment

Jennifer_MorrissetteJennifer Morrissette, Ph.D., Scientific Director, Clinical Cytogenetics Laboratory; Clinical Director, Center for Personalized Diagnostics (CPD), University of Pennsylvania School of Medicine

The routine implementation of NGS in the diagnosis and decision making process of malignancies has increasingly become part of routine clinical care. For most malignancies tested, only one specimen is received; however there are increasingly more patients assessed at additional time points throughout their disease allowing for tracking of the response to targeted and traditional chemotherapy. In hematological malignancies there has been the greatest uptake for the use of NGS to track disease response, as these patients can be serially assessed. This talk will focus on the mutation patterns before and after treatment and patient response to targeted therapies, on clinical trials and off-label.

GenPro12:30 Non-Bisulfite Epigenetic Profiling of DNA Methylation Enables Epigenetic Diagnostic Biomarker Discovery

Adam Marsh, Ph.D., CSO, Genome Profiling LLC

We have developed a novel computational platform for NGS quantitative DNA methylation profiling. This diagnostic platform has great utility for CDx applications. A use case in patients with Acute Myeloid Leukemia shows high confidence in pre-treatment identification of responders and non-responders to a hypomethylating drug therapy.

12:45 Sponsored Presentation (Opportunity Available)

QuarkBio1:00 Luncheon Presentation: Elucidation of Circulating miRNA Biomarkers in Oral Cavity Cancer Utilizing Multiplex qPCR Array

Eric Yang, Ph.D., Vice President, Quark Biosciences

Numerous publications have illustrated that microRNAs can be of diagnostic, prognostic and predictive values in the field of oncology. Utilizing PanelChip™, a qPCR array technology developed by Quark Biosciences, we’ve looked at the level of 160 microRNAs in the plasma of oral cavity cancer patients before and after surgery. Preliminary result suggests that PanelChip™ can be of a powerful tool to screen and validate cell-free miRNA biomarkers for translational medical researchers.

1:30 Session Break

EXAMINING CLINICAL UTILITY

2:00 Chairperson’s Remarks

Chair to be Announced, Stilla Technologies

2:05 Crucial Considerations for Pipelines to Validate Circulating Biomarkers for Breast Cancer

Karen_AndersonKaren Anderson, M.D., Ph.D., Associate Professor, School of Life Sciences, Biodesign Institute, Arizona State University

There has been significant progress in the discovery of potential circulating biomarkers, including proteins, autoantibodies, nucleic acids, exosomes, and circulating tumor cells, but the vast majority of these biomarkers have not progressed beyond initial research discovery, and none have yet been approved for clinical use in early stage disease. We will review the crucial considerations of developing pipelines for the rapid evaluation of circulating biomarkers for breast cancer.

2:35 Germline Findings in Tumor-Only Sequencing

Victoria_RaymondVictoria M. Raymond, Adjunct Clinical Assistant Professor, Licensed Certified Genetic Counselor, Cancer Genetics Clinic, University of Michigan Health System

Precision oncology holds great potential to improve patient outcomes. Tumor sequencing is rapidly moving into clinical care as our understanding of the cancer genome and the availability of targeted therapies increase. Analysis of the cancer genome is most informative when paired with germline DNA to delineate inherited and somatic variants. Although tumor-only analysis remains the most common methodology, it holds the potential to identify clinically significant germline variants.

3:05 Refreshment Break

3:35 PANEL DISCUSSION: Clinical Utility and Cost Effectiveness of Predictive Cancer Biomarkers

Moderator: Michael J. Heller, Ph.D., Professor, Bioengineering & Nanoengineering, University of California San Diego

Panelists: David Hoon, Ph.D., Director of the John Wayne Cancer Institute

Raj Krishnan, Ph.D., CEO, Biological Dynamics

Christos Hatzis, PhD, Assistant Professor, Medicine; Director, Bioinformatics, Breast Medical Oncology, Yale Comprehensive Cancer Center, Yale School of Medicine

Among other things, the use of a cancer biomarker is determined by its predictive value and any cost saving that enables a physician to better determine treatment, to modify or change treatment and which increases the likelihood of patient survival. Information that allows switching the patient from an expensive, ineffective, or toxic therapy earlier or faster will be most beneficial. On the other side of the equation is the intrinsic cost of the assay or technology required to detect and analyze the biomarker(s). Improvements in this area will allow better cost to benefit ratios, and lead to more widespread use of the biomarker in cancer diagnostics.

4:35 Close of Symposium

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