Archive for the ‘Brain Basis of Emotion’ Category

Healing traumatic brain injuries with self-assembling peptide hydrogels

Reporter : Irina Robu, PhD

In 2014, TBIs resulted in about 2.53 million emergency department visits in the U.S., according to the Centers for Disease Control and Prevention. A traumatic brain injury (TBI) can range from a mild concussion to a severe head injury. It is caused by a blow to the head or body, a wound that breaks through the skull or another injury that jars or shakes the brain. Individuals with traumatic brain injuries can develop secondary disorders after the initial blow. Researchers, Biplab Sarkar and Vivek Kumar from New Jersey Institute of Technology are hoping to prevent secondary disorders by injecting a self-assembling peptide hydrogel into the brains of rats with traumatic brain injury and see what happens. They observed that the hydrogel helped blood vessels regrow in addition to neuronal survival.

The researchers explained that after traumatic brain injury, the brain can amass glutamate which kills some neurons which is marked by overactive oxygen-containing molecules (oxidative stress), inflammation and disruption of the blood-brain barrier. Furthermore, TBI survivors can experience impaired motor control and depression. Within the experiment, the researchers showed that a week after injecting the gel in rats, the neurons have twice as many neurons at the injury site than the control animals did.

The NJIT researchers distinguished that they needed to inject the hydrogel directly in a rat’s brain just seconds after a TBI, which is not ideal, because it would be impossible to give a patient the treatment within that short period of time. The next step in showing that the self-assembling peptide hydrogel works is to combine their previous blood vessel-growing peptide and the new version to see whether it could enhance recovery. And the researchers plan to inspect whether the hydrogels work for more diffuse brain injuries such as concussions.



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Lesson 4 Cell Signaling And Motility: G Proteins, Signal Transduction: Curations and Articles of reference as supplemental information: #TUBiol3373

Curator: Stephen J. Williams, Ph.D.

Updated 7/15/2019

Below please find the link to the Powerpoint presentation for lesson #4 for #TUBiol3373.  The lesson first competes the discussion on G Protein Coupled Receptors, including how cells terminate cell signals.  Included are mechanisms of receptor desensitization.  Please NOTE that desensitization mechanisms like B arrestin decoupling of G proteins and receptor endocytosis occur after REPEATED and HIGH exposures to agonist.  Hydrolysis of GTP of the alpha subunit of G proteins, removal of agonist, and the action of phosphodiesterase on the second messenger (cAMP or cGMP) is what results in the downslope of the effect curve, the termination of the signal after agonist-receptor interaction.


Click below for PowerPoint of lesson 4

Powerpoint for lesson 4


Please Click below for the papers for your Group presentations

paper 1: Membrane interactions of G proteins and other related proteins

paper 2: Macaluso_et_al-2002-Journal_of_Cellular_Physiology

paper 3: Interactions of Ras proteins with the plasma membrane

paper 4: Futosi_et_al-2016-Immunological_Reviews


Please find related article on G proteins and Receptor Tyrosine Kinases on this Open Access Online Journal

G Protein–Coupled Receptor and S-Nitrosylation in Cardiac Ischemia and Acute Coronary Syndrome

Action of Hormones on the Circulation

Newer Treatments for Depression: Monoamine, Neurotrophic Factor & Pharmacokinetic Hypotheses

VEGF activation and signaling, lysine methylation, and activation of receptor tyrosine kinase


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Reporter and Curator: Dr. Sudipta Saha, Ph.D.


The relationship between gut microbial metabolism and mental health is one of the most intriguing and controversial topics in microbiome research. Bidirectional microbiota–gut–brain communication has mostly been explored in animal models, with human research lagging behind. Large-scale metagenomics studies could facilitate the translational process, but their interpretation is hampered by a lack of dedicated reference databases and tools to study the microbial neuroactive potential.


Out of all the many ways, the teeming ecosystem of microbes in a person’s gut and other tissues might affect health. But, its potential influences on the brain may be the most provocative for research. Several studies in mice had indicated that gut microbes can affect behavior, and small scale studies on human beings suggested this microbial repertoire is altered in depression. Studies by two large European groups have found that several species of gut bacteria are missing in people with depression. The researchers can’t say whether the absence is a cause or an effect of the illness, but they showed that many gut bacteria could make substances that affect the nerve cell function—and maybe the mood.


Butyrate-producing Faecalibacterium and Coprococcus bacteria were consistently associated with higher quality of life indicators. Together with DialisterCoprococcus spp. was also depleted in depression, even after correcting for the confounding effects of antidepressants. Two kinds of microbes, Coprococcus and Dialister, were missing from the microbiomes of the depressed subjects, but not from those with a high quality of life. The researchers also found the depressed people had an increase in bacteria implicated in Crohn disease, suggesting inflammation may be at fault.


Looking for something that could link microbes to mood, researchers compiled a list of 56 substances important for proper functioning of nervous system that gut microbes either produce or break down. They found, for example, that Coprococcus seems to have a pathway related to dopamine, a key brain signal involved in depression, although they have no evidence how this might protect against depression. The same microbe also makes an anti-inflammatory substance called butyrate, and increased inflammation is implicated in depression.


Still, it is very much unclear that how microbial compounds made in the gut might influence the brain. One possible channel is the vagus nerve, which links the gut and brain. Resolving the microbiome-brain connection might lead to novel therapies. Some physicians and companies are already exploring typical probiotics, oral bacterial supplements, for depression, although they don’t normally include the missing gut microbes identified in the new study.














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Understanding of the Brain Basis of Emotion: Capture the Emotional States that underlie Moods, Brain Activity and Expressive Signals – Berkeley Study in PNAS

Reporter: Aviva Lev-Ari, PhD, RN



Self-report captures 27 distinct categories of emotion bridged by continuous gradients

  1. Alan S. Cowena,1 and
  2. Dacher Keltnera




Claims about how reported emotional experiences are geometrically organized within a semantic space have shaped the study of emotion. Using statistical methods to analyze reports of emotional states elicited by 2,185 emotionally evocative short videos with richly varying situational content, we uncovered 27 varieties of reported emotional experience. Reported experience is better captured by categories such as “amusement” than by ratings of widely measured affective dimensions such as valence and arousal. Although categories are found to organize dimensional appraisals in a coherent and powerful fashion, many categories are linked by smooth gradients, contrary to discrete theories. Our results comprise an approximation of a geometric structure of reported emotional experience.


Emotions are centered in subjective experiences that people represent, in part, with hundreds, if not thousands, of semantic terms. Claims about the distribution of reported emotional states and the boundaries between emotion categories—that is, the geometric organization of the semantic space of emotion—have sparked intense debate. Here we introduce a conceptual framework to analyze reported emotional states elicited by 2,185 short videos, examining the richest array of reported emotional experiences studied to date and the extent to which reported experiences of emotion are structured by discrete and dimensional geometries. Across self-report methods, we find that the videos reliably elicit 27 distinct varieties of reported emotional experience. Further analyses revealed that categorical labels such as amusement better capture reports of subjective experience than commonly measured affective dimensions (e.g., valence and arousal). Although reported emotional experiences are represented within a semantic space best captured by categorical labels, the boundaries between categories of emotion are fuzzy rather than discrete. By analyzing the distribution of reported emotional states we uncover gradients of emotion—from anxiety to fear to horror to disgust, calmness to aesthetic appreciation to awe, and others—that correspond to smooth variation in affective dimensions such as valence and dominance. Reported emotional states occupy a complex, high-dimensional categorical space. In addition, our library of videos and an interactive map of the emotional states they elicit (https://s3-us-west-1.amazonaws.com/emogifs/map.html) are made available to advance the science of emotions.



Semantic atlas of human emotions

Three separate groups of study participants watched sequences of videos, and, after viewing each clip, completed a reporting task. The first group freely reported their emotional responses to each of 30 video clips.

“Their responses reflected a rich and nuanced array of emotional states, ranging from nostalgia to feeling ‘grossed out,’” Cowen said.

The second group ranked each video according to how strongly it made them feel admiration, adoration, aesthetic appreciation, amusement, anger, anxiety, awe, awkwardness, boredom, calmness, confusion, contempt, craving, disappointment, disgust, empathic pain, entrancement, envy, excitement, fear, guilt, horror, interest, joy, nostalgia, pride, relief, romance, sadness, satisfaction, sexual desire, surprise, sympathy and triumph.

Here, the experimenters found that participants converged on similar responses, with more than half of the viewers reporting the same category of emotion for each video.

The final cohort rated their emotional responses on a scale of 1 to 9 to each of a dozen videos based on such dichotomies as positive versus negative, excitement versus calmness, and dominance versus submissiveness. Researchers were able to predict how participants would score the videos based on how previous participants had assessed the emotions the videos elicited.


Human nature is often portrayed as selfish and power hungry, but research by Dacher Keltner finds that we are hard-wired to be kind.
Credit: Fig. 1 by University of California



Emoji fans take heart: Scientists pinpoint 27 states of emotion Friday, September 8, 2017



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Researchers Create A Simulated Mouse Brain in a Virtual Mouse Body

Reporter: Aviva Lev-Ari, PhD, RN

scientist Marc-Oliver Gewaltig and his team at the Human Brain Project (HBP) built a model mouse brain and a model mouse body, integrating them both into a single simulation and providing a simplified but comprehensive model of how the body and the brain interact with each other. “Replicating sensory input and motor output is one of the best ways to go towards a detailed brain model analogous to the real thing,” explains Gewaltig.


As computing technology improves, their goal is to build the tools and the infrastructure that will allow researchers to perform virtual experiments on mice and other virtual organisms. This virtual neurorobotics platform is just one of the collaborative interfaces being developed by the HBP. A first version of the software will be released to collaborators in April. The HBP scientists used biological data about the mouse brain collected by the Allen Brain Institute in Seattle and the Biomedical Informatics Research Network in San Diego. These data contain detailed information about the positions of the mouse brain’s 75 million neurons and the connections between different regions of the brain. They integrated this information with complementary data on the shapes, sizes and connectivity of specific types of neurons collected by the Blue Brain Project in Geneva.


A simplified version of the virtual mouse brain (just 200,000 neurons) was then mapped to different parts of the mouse body, including the mouse’s spinal cord, whiskers, eyes and skin. For instance, touching the mouse’s whiskers activated the corresponding parts of the mouse sensory cortex. And they expect the models to improve as more data comes in and gets incorporated. For Gewaltig, building a virtual organism is an exercise in data integration. By bringing together multiple sources of data of varying detail into a single virtual model and testing this against reality, data integration provides a way of evaluating – and fostering – our own understanding of the brain. In this way, he hopes to provide a big picture of the brain by bringing together separated data sets from around the world. Gewaltig compares the exercise to the 15th century European data integration projects in geography, when scientists had to patch together known smaller scale maps. These first attempts were not to scale and were incomplete, but the resulting globes helped guide further explorations and the development of better tools for mapping the Earth, until reaching today’s precision.


Read more: https://www.humanbrainproject.eu
Human Brain Project: http://www.humanbrainproject.eu
NEST simulator software : http://nest-simulator.org/
Largest neuronalnetwork simulation using NEST : http://bit.ly/173mZ5j

Open Source Data Sets:
Allen Institute for Brain Science: http://www.brain-map.org
Bioinformatics Research Network (BIRN): http://www.birncommunity.org

The Behaim Globe :
Germanisches National Museum, http://www.gnm.de/
Department of Geodesy and Geoinformation, TU Wien, http://www.geo.tuwien.ac.at

Source: www.33rdsquare.com

See on Scoop.itCardiovascular Disease: PHARMACO-THERAPY

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