Souza, the founder of n3D Biosciences states that microtissues grown with cell levitation have morphology closer to in vivo tissues than conventional cell culture. According to Souza “One huge hurdle is the standardization of the handling of tissue culture cells.  Ideally, new tissues for a patient wouldbe grown from their own cells, avoiding the issues of “rejection” of tissue as not the body’s own.  One problem is that cells are very sensitive to changes in temperature during the freezing, thawing, and recovery process. ”