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Archive for the ‘3D Plotting Scaffolds’ Category

Nanoparticles Used to Levitate Cells in Tissue Culture

Posted in 3D Plotting Scaffolds, 3D Printing for Medical Application, Biological Engineering, Biopolymer Blend Open Porous, Nanotechnology for Drug Delivery, tagged , , , , , , , on January 3, 2016| Leave a Comment »

Levitating Cells with Nanoparticles

Reporter: Irina Robu

 

VIEW VIDEO

 

n3Dbio’s Bioassembler

Technology: magnetic levitation
Materials: cells
Price: $500-750 kits

n3d-bioassembler bioprinting 3D printing industry
In magnetic levitation, cells are magnetized with NanoShuttle-PL (which consists of gold, iron oxide, and poly-L-lysine and magnetizes cells by electrostatically attaching to cell membranes) through overnight incubation and dispensed into a cell-repellent, multiwell plate, where they are levitated off the bottom of the dish by a magnet above the plate. In levitating cells off the bottom of a multiwell plate, the magnetic forces work as an invisible scaffold that rapidly aggregates cells, and induces cell-cell interactions and ECM synthesis. The 3D culture is formed without any artificial substrate or specialized media or equipment and can be cultured long-term. Additionally, adding and removing solutions is made easy by the use of magnets to hold down 3D cultures when removing solutions, limiting culture loss. 3D cultures can also be picked up and transferred between vessels using magnetic tools such as the MagPen.

SOURCE

http://3dprintingindustry.com/2015/08/26/top-10-bioprinters/?utm_source=3D+Printing+Industry+Update&utm_medium=email&utm_campaign=a02be27373-RSS_EMAIL_CAMPAIGN&utm_term=0_695d5c73dc-a02be27373-64568677

 

Cell culture is essential tool in drug discovery, tissue engineering and stem cell research.  Conventional tissue culture produces a two dimensional cell growth with gene expression, signalling and morphology that can be different from those found in vivo. In some cases, cells may or may not adhere to the tissue culture dishes.

Technology developed by n3D Biosciences brings cells together in a dish using magnetic nanoparticles to levitate them in a magnetic field. This procedure allows cells to develop into spheroids, but if they touch the bottom of the dish, they spread out in a single layer.

The technology developed by n3D Biosciences provide an alternative to biodegradable porous scaffold and protein matrices. Conventionally, biodegradable scaffold may suffer from slow or delayed propagation of cells and establishment of cell-cell interactions. The technology allows adaptable magnetic-based cell levitation and can provide an improved three-dimensional cell growth condition in certain settings.

To achieve this, the first step is to attach magnetic nanoparticles to the cell’s surface which is done by crosslinking the gold and iron oxide nanoparticles with polylysine.  In the second step, the cells are brought together while levitating them off the dish. In this condition, the cells can develop in surroundings mirroring growth inside a body where the cells are in contact with each other.  The media exchange is also relatively simple because a magnet holds the tissue in place.

 

Souza, the founder of n3D Biosciences states that microtissues grown with cell levitation have morphology closer to in vivo tissues than conventional cell culture. According to Souza “One huge hurdle is the standardization of the handling of tissue culture cells.  Ideally, new tissues for a patient wouldbe grown from their own cells, avoiding the issues of “rejection” of tissue as not the body’s own.  One problem is that cells are very sensitive to changes in temperature during the freezing, thawing, and recovery process. ”

Source

http://www.biocision.com/blog/7459/levitating-cells-nanoparticles

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Materialise Partners with University of Michigan and Tissue Regeneration Services for Clinical Trials of 3D Printed Tracheal Splint

Posted in 3D Plotting Scaffolds, 3D Printing for Medical Application, Biopolymer Blend Open Porous, tagged , , , on December 26, 2015| Leave a Comment »

Materialise Partners with University of Michigan and Tissue Regeneration Services for Clinical Trials of 3D Printed Tracheal Splint

Reporter: Irina Robu, PhD

Dr. Scott Hollister, a biomedical engineering professor at University of Michigan and Dr. Glenn Green, otolaryngologit at C.S. Mott Children’s Hospital invented a tracheal splint using 3D printing in 2012. The 3D printed trachea of a baby with tracheobronchomalacia (TBM),keeps the airway open until it can grow into a healty state and stay open on its own.  The splint dissolves and is absorbed in the body and the process can take up to three years. Dr. Hollister and Dr. Green partnered with Materialise and Tissue Regeneration systems to commercialize the device, starting with clinical trial involving involving 30 patients at Mott Children’s Hospital sometime next year.

According to Dr. Green“This agreement is a critical step in our goal to make this treatment readily available for other children who suffer from this debilitating condition.We have continued to evolve and automate the design process for the splints, allowing us to achieve in two days what used to take us up to five days to accomplish. I feel incredibly privileged to be building products that surgeons can use to save lives.”

The bioresorbable splints will be manufactured by Plymouth, Michigan startup Tissue Regeneration Systems, which received its first commercial product clearance from the FDA in 2013 after several years of product development.

SOURCE
http://3dprint.com/109725/materialise-uom-trs-partners/

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Lab Grown Brains and more from Twittersphere on 3D Bio-Printing News

Posted in 3D Plotting Scaffolds, 3D Printing for Medical Application, 4D Printing and Meta Materials, BioPrinting in Regenerative Medicine, tagged , , , , , , , , , on November 16, 2015| Leave a Comment »

Lab Grown Brains and more from Twittersphere on 3D Bio-Printing News

Curator: Stephen J. Williams, Ph.D

How Tiny Lab-Grown Human Brains Are Giving Big Insights Into Autism and more from the Twittershpere

 

https://twitter.com/singularityhub/status/664508353771610112

(more…)

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FDA Guidance on Use of Xenotransplanted Products in Human: Implications in 3D Printing

Posted in 3D Plotting Scaffolds, 3D Printing for Medical Application, BioInks, Biopolymer Blend Open Porous, BioPrinting in Regenerative Medicine, Cell Level, FDA, FDA Regulatory Affairs, MicroEngineering Cell-Tissue & Systems, tagged , , , , , , , , , , , , , on November 9, 2015| Leave a Comment »

FDA Guidance On Source Animal, Product, Preclinical and Clinical Issues Concerning the Use of Xenotranspantation Products in Humans – Implications for 3D BioPrinting of Regenerative Tissue

Reporter: Stephen J. Williams, Ph.D.

 

The FDA has submitted Final Guidance on use xeno-transplanted animal tissue, products, and cells into human and their use in medical procedures. Although the draft guidance was to expand on previous guidelines to prevent the introduction, transmission, and spread of communicable diseases, this updated draft may have implications for use of such tissue in the emerging medical 3D printing field.

This document is to provide guidance on the production, testing and evaluation of products intended for use in xenotransplantation. The guidance includes scientific questions that should be addressed by sponsors during protocol development and during the preparation of submissions to the Food and Drug Administration (FDA), e.g., Investigational New Drug Application (IND) and Biologics License Application (BLA). This guidance document finalizes the draft guidance of the same title dated February 2001.

For the purpose of this document, xenotransplantation refers to any procedure that involves the transplantation, implantation, or infusion into a human recipient of either (a) live cells, tissues, or organs from a nonhuman animal source, or (b) human body fluids, cells, tissues or organs that have had ex vivo contact with live nonhuman animal cells, tissues or organs. For the purpose of this document, xenotransplantation products include live cells, tissues or organs used in xenotransplantation. (See Definitions in section I.C.)

This document presents issues that should be considered in addressing the safety of viable materials obtained from animal sources and intended for clinical use in humans. The potential threat to both human and animal welfare from zoonotic or other infectious agents warrants careful characterization of animal sources of cells, tissues, and organs. This document addresses issues such as the characterization of source animals, source animal husbandry practices, characterization of xenotransplantation products, considerations for the xenotransplantation product manufacturing facility, appropriate preclinical models for xenotransplantation protocols, and monitoring of recipients of xenotransplantation products. This document recommends specific practices intended to prevent the introduction and spread of infectious agents of animal origin into the human population. FDA expects that new methods proposed by sponsors to address specific issues will be scientifically rigorous and that sufficient data will be presented to justify their use.

Examples of procedures involving xenotransplantation products include:

  • transplantation of xenogeneic hearts, kidneys, or pancreatic tissue to treat organ failure,
  • implantation of neural cells to ameliorate neurological degenerative diseases,
  • administration of human cells previously cultured ex vivo with live nonhuman animal antigen-presenting or feeder cells, and
  • extracorporeal perfusion of a patient’s blood or blood component perfused through an intact animal organ or isolated cells contained in a device to treat liver failure.

The guidance addresses issues such as:

  1. Clinical Protocol Review
  2. Xenotransplantation Site
  3. Criteria for Patient Selection
  4. Risk/Benefit Assessment
  5. Screening for Infectious Agents
  6. Patient Follow-up
  7. Archiving of Patient Plasma and Tissue Specimens
  8. Health Records and Data Management
  9. Informed Consent
  10. Responsibility of the Sponsor in Informing the Patient of New Scientific Information

A full copy of the PDF can be found below for reference:

fdaguidanceanimalsourcesxenotransplatntation

An example of the need for this guidance in conjunction with 3D printing technology can be understood from the below article (source http://www.geneticliteracyproject.org/2015/09/03/pig-us-xenotransplantation-new-age-chimeric-organs/)

Pig in us: Xenotransplantation and new age of chimeric organs

David Warmflash | September 3, 2015 | Genetic Literacy Project

Imagine stripping out the failing components of an old car — the engine, transmission, exhaust system and all of those parts — leaving just the old body and other structural elements. Replace those old mechanical parts with a brand new electric, hydrogen powered, biofuel, nuclear or whatever kind of engine you want and now you have a brand new car. It has an old frame, but that’s okay. The frame wasn’t causing the problem, and it can live on for years, undamaged.

When challenged to design internal organs, tissue engineers are taking a similar approach, particularly with the most complex organs, like the heart, liver and kidneys. These organs have three dimensional structures that are elaborate, not just at the gross anatomic level, but in microscopic anatomy too. Some day, their complex connective tissue scaffolding, the stroma, might be synthesized from the needed collagen proteins with advanced 3-D printing. But biomedical engineering is not there yet, so right now the best candidate for organ scaffolding comes from one of humanity’s favorite farm animals: the pig.

Chimera alarmists connecting with anti-biotechnology movements might cringe at the thought of building new human organs starting with pig tissue, but if you’re using only the organ scaffolding and building a working organ from there, pig organs may actually be more desirable than those donated by humans.

How big is the anti-chimerite movement?

Unlike anti-GMO and anti-vaccination activists, there really aren’t too many anti-chemerites around. Nevertheless, there is a presence on the web of people who express concern about mixing of humans and non-human animals. Presently, much of their concern is focussed on the growing of human organs inside non-human animals, pigs included. One anti-chemerite has written that it could be a problem for the following reason:

Once a human organ is grown inside a pig, that pig is no longer fully a pig. And without a doubt, that organ will no longer be a fully human organ after it is grown inside the pig. Those receiving those organs will be allowing human-animal hybrid organs to be implanted into them. Most people would be absolutely shocked to learn some of the things that are currently being done in the name of science.

The blog goes on to express alarm about the use of human genes in rice and from there morphs into an off the shelf garden variety anti-GMO tirade, though with an an anti-chemeric current running through it. The concern about making pigs a little bit human and humans a little bit pig becomes a concern about making rice a little bit human. But the concern about fusing tissues and genes of humans and other species does not fit with the trend in modern medicine.

Utilization of pig tissue enters a new age 

pigsinus

A porcine human ear for xenotransplantation. source: The Scientist

For decades, pig, bovine and other non-human tissues have been used in medicine. People are walking around with pig and cow heart valves. Diabetics used to get a lot of insulin from pigs and cows, although today, thanks to genetic engineering, they’re getting human insulin produced by microorganisms modified genetically to make human insulin, which is safer and more effective.

When it comes to building new organs from old ones, however, pig organs could actually be superior for a couple of reasons. For one thing, there’s no availability problem with pigs. Their hearts and other organs also have all of the crucial components of the extracellular matrix that makes up an organ’s scaffolding. But unlike human organs, the pig organs don’t tend to carry or transfer human diseases. That is a major advantage that makes them ideal starting material. Plus there is another advantage: typically, the hearts of human cadavers are damaged, either because heart disease is what killed the human owner or because resuscitation efforts aimed at restarting the heart of a dying person using electrical jolts and powerful drugs.

Rebuilding an old organ into a new one

How then does the process work? Whether starting with a donated human or pig organ, there are several possible methods. But what they all have in common is that only the scaffolding of the original organ is retained. Just like the engine and transmission of the old car, the working tissue is removed, usually using detergents. One promising technique that has been applied to engineer new hearts is being tested by researchers at the University of Pittsburgh. Detergents pumped into the aorta attached to a donated heart (donated by a human cadaver, or pig or cow). The pressure keeps the aortic valve closed, so the detergents to into the coronary arteries and through the myocardial (heart muscle) and endocardial (lining over the muscle inside the heart chambers) tissue, which thus gets dissolved over the course of days. What’s left is just the stroma tissue, forming a scaffold. But that scaffold has signaling factors that enable embryonic stem cells, or specially programed adult pleuripotent cells to become all of the needed cells for a new heart.

Eventually, 3-D printing technology may reach the point when no donated scaffolding is needed, but that’s not the case quite yet, plus with a pig scaffolding all of the needed signaling factors are there and they work just as well as those in a human heart scaffold. All of this can lead to a scenario, possibly very soon, in which organs are made using off-the-self scaffolding from pig organs, ready to produce a custom-made heart using stem or other cells donated by new organ’s recipient.

David Warmflash is an astrobiologist, physician, and science writer. Follow @CosmicEvolution to read what he is saying on Twitter.

And a Great Article in The Scientist by Dr. Ed Yong Entitled

Replacement Parts

To cope with a growing shortage of hearts, livers, and lungs suitable for transplant, some scientists are genetically engineering pigs, while others are growing organs in the lab.

By Ed Yong | August 1, 2012

Source: http://www.the-scientist.com/?articles.view/articleNo/32409/title/Replacement-Parts/

.. where Joseph Vacanti and David Cooper figured that using

“engineered pigs without the a-1,3-galactosyltransferase gene that produces the a-gal residues. In addition, the pigs carry human cell-membrane proteins such as CD55 and CD46 that prevent the host’s complement system from assembling and attacking the foreign cells”

… thereby limiting rejection of the xenotransplated tissue.

In addition to issues related to animal virus transmission the issue of optimal scaffolds for organs as well as the advantages which 3D Printing would have in mass production of organs is discussed:

To Vacanti, artificial scaffolds are the future of organ engineering, and the only way in which organs for transplantation could be mass-produced. “You should be able to make them on demand, with low-cost materials and manufacturing technologies,” he says. That is relatively simple for organs like tracheas or bladders, which are just hollow tubes or sacs. Even though it is far more difficult for the lung or liver, which have complicated structures, Vacanti thinks it will be possible to simulate their architecture with computer models, and fabricate them with modern printing technology. (See “3-D Printing,” The Scientist, July 2012.) “They obey very ordered rules, so you can reduce it down to a series of algorithms, which can help you design them,” he says. But Taylor says that even if the architecture is correct, the scaffold would still need to contain the right surface molecules to guide the growth of any added cells. “It seems a bit of an overkill when nature has already done the work for us,” she says.

Other articles of FDA Guidance and 3D Bio Printing on this Open Access Journal Include:

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New FDA Draft Guidance On Homologous Use of Human Cells, Tissues, and Cellular and Tissue-Based Products – Implications for 3D BioPrinting of Regenerative Tissue

Posted in 3D Plotting Scaffolds, 3D Printing for Medical Application, 4D Printing and Meta Materials, BioInks, Biopolymer Blend Open Porous, BioPrinting in Regenerative Medicine, Dental Applications, FDA, FDA Regulatory Affairs, MicroEngineering Cell-Tissue & Systems, Tissue Engineering, tagged , , , , , , , on November 8, 2015| Leave a Comment »

New FDA Draft Guidance On Homologous Use of Human Cells, Tissues, and Cellular and Tissue-Based Products – Implications for 3D BioPrinting of Regenerative Tissue

Reporter: Stephen J. Williams, Ph.D.

The FDA recently came out with a Draft Guidance on use of human cells, tissues and cellular and tissue-based products (HCT/P) {defined in 21 CFR 1271.3(d)} and their use in medical procedures. Although the draft guidance was to expand on previous guidelines to prevent the introduction, transmission, and spread of communicable diseases, this updated draft may have implications for use of such tissue in the emerging medical 3D printing field.

A full copy of the PDF can be found here for reference but the following is a summary of points of the guidance.FO508ver – 2015-373 HomologousUseGuidanceFinal102715

In 21 CFR 1271.10, the regulations identify the criteria for regulation solely under section 361 of the PHS Act and 21 CFR Part 1271. An HCT/P is regulated solely under section 361 of the PHS Act and 21 CFR Part 1271 if it meets all of the following criteria (21 CFR 1271.10(a)):

  • The HCT/P is minimally manipulated;
  • The HCT/P is intended for homologous use only, as reflected by the labeling, advertising, or other indications of the manufacturer’s objective intent;
  • The manufacture of the HCT/P does not involve the combination of the cells or tissues with another article, except for water, crystalloids, or a sterilizing, preserving, or storage agent, provided that the addition of water, crystalloids, or the sterilizing, preserving, or storage agent does not raise new clinical safety concerns with respect to the HCT/P; and
  • Either:
  1. The HCT/P does not have a systemic effect and is not dependent upon the metabolic activity of living cells for its primary function; or
  2. The HCT/P has a systemic effect or is dependent upon the metabolic activity of living cells for its primary function, and:
  3. Is for autologous use;
  4. Is for allogeneic use in a first-degree or second-degree blood relative; or
  5. Is for reproductive use.

If an HCT/P does not meet all of the criteria in 21 CFR 1271.10(a), and the establishment that manufactures the HCT/P does not qualify for any of the exceptions in 21 CFR 1271.15, the HCT/P will be regulated as a drug, device, and/or biological product under the Federal Food, Drug and Cosmetic Act (FD&C Act), and/or section 351 of the PHS Act, and applicable regulations, including 21 CFR Part 1271, and pre-market review will be required.

1 Examples of HCT/Ps include, but are not limited to, bone, ligament, skin, dura mater, heart valve, cornea, hematopoietic stem/progenitor cells derived from peripheral and cord blood, manipulated autologous chondrocytes, epithelial cells on a synthetic matrix, and semen or other reproductive tissue. The following articles are not considered HCT/Ps: (1) Vascularized human organs for transplantation; (2) Whole blood or blood components or blood derivative products subject to listing under 21 CFR Parts 607 and 207, respectively; (3) Secreted or extracted human products, such as milk, collagen, and cell factors, except that semen is considered an HCT/P; (4) Minimally manipulated bone marrow for homologous use and not combined with another article (except for water, crystalloids, or a sterilizing, preserving, or storage agent, if the addition of the agent does not raise new clinical safety concerns with respect to the bone marrow); (5) Ancillary products used in the manufacture of HCT/P; (6) Cells, tissues, and organs derived from animals other than humans; (7) In vitro diagnostic products as defined in 21 CFR 809.3(a); and (8) Blood vessels recovered with an organ, as defined in 42 CFR 121.2 that are intended for use in organ transplantation and labeled “For use in organ transplantation only.” (21 CFR 1271.3(d))

Contains Nonbinding Recommendations
Draft – Not for Implementation

Section 1271.10(a)(2) (21 CFR 1271.10(a)(2)) provides that one of the criteria for an HCT/P to be regulated solely under section 361 of the PHS Act is that the “HCT/P is intended for homologous use only, as reflected by the labeling, advertising, or other indications of the manufacturer’s objective intent.” As defined in 21 CFR 1271.3(c), homologous use means the repair, reconstruction, replacement, or supplementation of a recipient’s cells or tissues with an HCT/P that performs the same basic function or functions in the recipient as in the donor. This criterion reflects the Agency’s conclusion that there would be increased safety and effectiveness concerns for HCT/Ps that are intended for a non-homologous use, because there is less basis on which to predict the product’s behavior, whereas HCT/Ps for homologous use can reasonably be expected to function appropriately (assuming all of the other criteria are also met).2 In applying the homologous use criterion, FDA will determine what the intended use of the HCT/P is, as reflected by the the labeling, advertising, and other indications of a manufacturer’s objective intent, and will then apply the homologous use definition.

FDA has received many inquiries from manufacturers about whether their HCT/Ps meet the homologous use criterion in 21 CFR 1271.10(a)(2). Additionally, transplant and healthcare providers often need to know this information about the HCT/Ps that they are considering for use in their patients. This guidance provides examples of different types of HCT/Ps and how the regulation in 21 CFR 1271.10(a)(2) applies to them, and provides general principles that can be applied to HCT/Ps that may be developed in the future. In some of the examples, the HCT/Ps may fail to meet more than one of the four criteria in 21 CFR 1271.10(a).

III. QUESTIONS AND ANSWERS

  1. What is the definition of homologous use?

Homologous use means the repair, reconstruction, replacement, or supplementation of a recipient’s cells or tissues with an HCT/P that performs the same basic function or functions in the recipient as in the donor (21 CFR 1271.3(c)), including when such cells or tissues are for autologous use. We generally consider an HCT/P to be for homologous use when it is used to repair, reconstruct, replace, or supplement:

  • Recipient cells or tissues that are identical (e.g., skin for skin) to the donor cells or tissues, and perform one or more of the same basic functions in the recipient as the cells or tissues performed in the donor; or,
  • Recipient cells that may not be identical to the donor’s cells, or recipient tissues that may not be identical to the donor’s tissues, but that perform one or more of the same basic functions in the recipient as the cells or tissues performed in the donor.3

2 Proposed Approach to Regulation of Cellular and Tissue-Based Products, FDA Docket. No. 97N-0068 (February. 28, 1997) page 19. http://www.fda.gov/downloads/biologicsbloodvaccines/guidancecomplianceregulatoryinformation/guidances/tissue/ ucm062601.pdf.

3“Establishment Registration and Listing for Manufacturers of Human Cellular and Tissue-Based Products” 63 FR 26744 at 26749 (May 14, 1998).

Contains Nonbinding Recommendations
Draft – Not for Implementation

1-1. A heart valve is transplanted to replace a dysfunctional heart valve. This is homologous use because the donor heart valve performs the same basic function in the donor as in the recipient of ensuring unidirectional blood flow within the heart.

1-2. Pericardium is intended to be used as a wound covering for dura mater defects. This is homologous use because the pericardium is intended to repair or reconstruct the dura mater and serve as a covering in the recipient, which is one of the basic functions it performs in the donor.

Generally, if an HCT/P is intended for use as an unproven treatment for a myriad of

diseases or conditions, the HCT/P is likely not intended for homologous use only.4

  1. What does FDA mean by repair, reconstruction, replacement, or supplementation of a recipient’s cells or tissues?

Repair generally means the physical or mechanical restoration of tissues, including by covering or protecting. For example, FDA generally would consider skin removed from a donor and then transplanted to a recipient in order to cover a burn wound to be a homologous use. Reconstruction generally means surgical reassembling or re-forming. For example, reconstruction generally would include the reestablishment of the physical integrity of a damaged aorta.5 Replacement generally means substitution of a missing tissue or cell, for example, the replacement of a damaged or diseased cornea with a healthy cornea or the replacement of donor hematopoietic stem/progenitor cells in a recipient with a disorder affecting the hematopoietic system that is inherited, acquired, or the result of myeloablative treatment. Supplementation generally means to add to, or complete. For example, FDA generally would consider homologous uses to be the implantation of dermal matrix into the facial wrinkles to supplement a recipient’s tissues and the use of bone chips to supplement bony defects. Repair, reconstruction, replacement, and supplementation are not mutually exclusive functions and an HCT/P could perform more than one of these functions for a given intended use.

  1. What does FDA mean by “the same basic function or functions” in the definition of homologous use?

For the purpose of applying the regulatory framework, the same basic function or functions of HCT/Ps are considered to be those basic functions the HCT/P performs in the body of the donor, which, when transplanted, implanted, infused, or transferred, the HCT/P would be expected to perform in the recipient. It is not necessary for the HCT/P in the recipient to perform all of the basic functions it performed in the donor, in order to

4 “Human Cells, Tissues, and Cellular and Tissue-Based Products; Establishment Registration and Listing” 66 FR 5447 at 5458 (January 19, 2001).

5 “Current Good Tissue Practice for Human Cell, Tissue, and Cellular and Tissue-Based Product Establishments; Inspection and Enforcement” 69 FR 68612 at 68643 (November 24, 2004) states, “HCT/Ps with claims for “reconstruction or repair” can be regulated solely under section 361 of the PHS Act, provided the HCT/P meets all the criteria in § 1271.10, including minimal manipulation and homologous use.”

Contains Nonbinding Recommendations
Draft – Not for Implementation

meet the definition of homologous use. However, to meet the definition of homologous use, any of the basic functions that the HCT/P is expected to perform in the recipient must be a basic function that the HCT/P performed in the donor.

A homologous use for a structural tissue would generally be to perform a structural function in the recipient, for example, to physically support or serve as a barrier or conduit, or connect, cover, or cushion.

A homologous use for a cellular or nonstructural tissue would generally be a metabolic or biochemical function in the recipient, such as, hematopoietic, immune, and endocrine functions.

3-1. The basic functions of hematopoietic stem/progenitor cells (HPCs) include to form and to replenish the hematopoietic system. Sources of HPCs include cord blood, peripheral blood, and bone marrow.6

  1. HPCs derived from peripheral blood are intended for transplantation into an individual with a disorder affecting the hematopoietic system that is inherited, acquired, or the result of myeloablative treatment. This is homologous use because the peripheral blood product performs the same basic function of reconstituting the hematopoietic system in the recipient.
  2. HPCs derived from bone marrow are infused into an artery with a balloon catheter for the purpose of limiting ventricular remodeling following acute myocardial infarction. This is not homologous use because limiting ventricular remodeling is not a basic function of bone marrow.
  3. A manufacturer provides HPCs derived from cord blood with a package insert stating that cord blood may be infused intravenously to differentiate into neuronal cells for treatment of cerebral palsy. This is not homologous use because there is insufficient evidence to support that such differentiation is a basic function of these cells in the donor.

3-2. The basic functions of the cornea include protecting the eye by forming its outermost layer and serving as the refracting medium of the eye. A corneal graft is transplanted to restore sight in a patient with corneal blindness. This is homologous use because a corneal graft performs the same basic functions in the donor as in the recipient.

3-3. The basic functions of a vein or artery include serving as a conduit for blood flow throughout the body. A cryopreserved vein or artery is used for arteriovenous access during hemodialysis. This is homologous use because the vein or artery is supplementing the vessel as a conduit for blood flow.

3-4. The basic functions of amniotic membrane include covering, protecting, serving as a selective barrier for the movement of nutrients between the external and in utero

6 Bone marrow meets the definition of an HCT/P only if is it more than minimally manipulated; intended by the manufacturer for a non-homologous use, or combined with certain drugs or devices.

Contains Nonbinding Recommendations
Draft – Not for Implementation

environment, and to retain fluid in utero. Amniotic membrane is used for bone tissue replacement to support bone regeneration following surgery to repair or replace bone defects. This is not a homologous use because bone regeneration is not a basic function of amniotic membrane.

3-5. The basic functions of pericardium include covering, protecting against infection, fixing the heart to the mediastinum, and providing lubrication to allow normal heart movement within chest. Autologous pericardium is used to replace a dysfunctional heart valve in the same patient. This is not homologous use because facilitating unidirectional blood flow is not a basic function of pericardium.

  1. Does my HCT/P have to be used in the same anatomic location to perform the same basic function or functions?

An HCT/P may perform the same basic function or functions even when it is not used in the same anatomic location where it existed in the donor.7 A transplanted HCT/P could replace missing tissue, or repair, reconstruct, or supplement tissue that is missing or damaged, either when placed in the same or different anatomic location, as long as it performs the same basic function(s) in the recipient as in the donor.

4-1. The basic functions of skin include covering, protecting the body from external force, and serving as a water-resistant barrier to pathogens or other damaging agents in the external environment. The dermis is the elastic connective tissue layer of the skin that provides a supportive layer of the integument and protects the body from mechanical stress.

  1. An acellular dermal product is used for supplemental support, protection, reinforcement, or covering for a tendon. This is homologous use because in both anatomic locations, the dermis provides support and protects the soft tissue structure from mechanical stress.
  2. An acellular dermal product is used for tendon replacement or repair. This is not homologous use because serving as a connection between muscle and bone is not a basic function of dermis.

4-2. The basic functions of amniotic membrane include serving as a selective barrier for the movement of nutrients between the external and in utero environment and to retain fluid in utero. An amniotic membrane product is used for wound healing of dermal ulcers and defects. This is not homologous use because wound healing of dermal lesions is not a basic function of amniotic membrane.

4-3. The basic functions of pancreatic islets include regulating glucose homeostasis within the body. Pancreatic islets are transplanted into the liver through the portal vein,

7 “Human Cells, Tissues, and Cellular and Tissue-Based Products; Establishment Registration and Listing” 66 FR 5447 at 5458 (January 19, 2001).

6

Contains Nonbinding Recommendations
Draft – Not for Implementation

for preservation of endocrine function after pancreatectomy. This is homologous use because the regulation of glucose homeostasis is a basic function of pancreatic islets.

  1. What does FDA mean by “intended for homologous use” in 21 CFR 1271.10(a)(2)?

The regulatory criterion in 21 CFR 1271.10(a)(2) states that the HCT/P is intended for homologous use only, as reflected by the labeling, advertising, or other indications of the manufacturer’s objective intent.

Labeling includes the HCT/P label and any written, printed, or graphic materials that supplement, explain, or are textually related to the product, and which are disseminated by or on behalf of its manufacturer.8 Advertising includes information, other than labeling, that originates from the same source as the product and that is intended to supplement, explain, or be textually related to the product (e.g., print advertising, broadcast advertising, electronic advertising (including the Internet), statements of company representatives).9

An HCT/P is intended for homologous use when its labeling, advertising, or other indications of the manufacturer’s objective intent refer to only homologous uses for the HCT/P. When an HCT/P’s labeling, advertising, or other indications of the manufacturer’s objective intent refer to non-homologous uses, the HCT/P would not meet the homologous use criterion in 21 CFR 1271.10(a)(2).

  1. What does FDA mean by “manufacturer’s objective intent” in 21 CFR 1271.10(a)(2)?

A manufacturer’s objective intent is determined by the expressions of the manufacturer or its representatives, or may be shown by the circumstances surrounding the distribution of the article. A manufacturer’s objective intent may, for example, be shown by labeling claims, advertising matter, or oral or written statements by the manufacturer or its representatives. It may be shown by the circumstances that the HCT/P is, with the knowledge of the manufacturer or its representatives, offered for a purpose for which it is neither labeled nor advertised.

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3-D Printing Overview

Posted in 3D Plotting Scaffolds, 3D Printing for Medical Application, BioInks, Dental Applications, tagged on November 8, 2015| Leave a Comment »

3-D Medical BioPrinting Overview

Curator: Larry H. Bernstein, MD, FCAP

 

 

Osteo3D Opens Marketplace for 3D Printable Medical Models from Real Patient Data   

 BY ON FRI, JULY 24, 2015

 

There are many companies dedicated to improving the use of 3D printing in the medical sector, and one of them Osteo3D, just released an amazing online repository of medical 3D models. Of course, patient information is private, so some of the “live models” have been made anonymous.  But there are now over 100 3D models available to doctors and other registered medical professionals online, which allows them t0 compare their own patient’s 3D files with Osteo3D models.  In fact, they can 3D print these models at home or wherever they have access to a 3D printer.

But, if a doctor or medical student doesn’t have a 3D printer, they can order a print directly from Osteo3D.  The online platform is powered by cloud printing site df3d and is meant to increase awareness of the usefulness of 3D printing among healthcare professionals.  Osteo3D hopes that, by collaborating with healthcare professionals, the tech industry, and academic institutions, they will help facilitate access and lower costs for the use of 3D printing in the healthcare field.

Currently the models are divided into the following categories:

  • Head and neck
  • Spine
  • Chest
  • Abdomen and pelvis
  • Extremities

When you click on 3D view, you don’t get quite the same level of control and depth asSketchfab, and some of the models look like pixels are growing out of them, but I didn’t pay to download any of the models, so I can’t say for sure. They are currently on the lookout for 3D printing partners who can help them deliver these 3D prints from their cloud platform to their customers around the world.

 

Osteo3d-Models-Store-3d-printing-Medical-Models-Banner02

http://3dprintingindustry.com/wp-content/uploads/2015/07/Osteo3d-Models-Store-3d-printing-Medical-Models-Banner02-1024×337.jpg

 

We’ve covered a number of stories that revolve around the combination of scanning/sensing technologies, great 3D modeling software, and 3D printing.  This combination has arguably had the most impact on the quality of human life as it is seen in the medical sector.  Diagnoses are made to a patient with a life-threatening condition.  Doctors, then, use 3D scanning technology to capture the reality data of a patient’s affected area, whether it be an organ, vasculature, bone structure, or combination of all three.  Next, a 3D model is created and manipulated using innovative software, and the model is 3D printed.  Surgery is practiced and visualized on the model, and then performed on the patient.  After doctors have all of this information, they use it to teach other doctors and nurse practitioners.  So, why not spread this wealth of information?  This is digital information, after all.  Any 3D model that is successfully used to print a 3D model of a diagnosed patient can be transferred anywhere, to anyone.

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Biofabrication with Stem Cells

Posted in 3D Plotting Scaffolds, 3D Printing for Medical Application, Artificial Vascular Structures, BioInks, MicroEngineering Cell-Tissue & Systems, tagged , , , on November 7, 2015| Leave a Comment »

Biofabrication with Stem Cells

Curator: Larry H. Bernstein, MD, FCAP

 

 

Biofabrication  Special Issue:  Dec 2015; 7(4).    http://iopscience.iop.org/1758-5090/7/4

 

Three-dimensional bioprinting of embryonic stem cells directs highly uniform embryoid body formation

Liliang Ouyang1,2,6, Rui Yao1,2,6, Shuangshuang Mao1,2, Xi Chen3, Jie Na3 and Wei Sun1,2,4,5
Biofabrication, Volume 7(4)    http://iopscience.iop.org/article/10.1088/1758-5090/7/4/044101/meta

With the ability to manipulate cells temporarily and spatially into three-dimensional (3D) tissue-like construct, 3D bioprinting technology was used in many studies to facilitate the recreation of complex cell niche and/or to better understand the regulation of stem cell proliferation and differentiation by cellular microenvironment factors. Embryonic stem cells (ESCs) have the capacity to differentiate into any specialized cell type of the animal body, generally via the formation of embryoid body (EB), which mimics the early stages of embryogenesis. In this study, extrusion-based 3D bioprinting technology was utilized for biofabricating ESCs into 3D cell-laden construct. The influence of 3D printing parameters on ESC viability, proliferation, maintenance of pluripotency and the rule of EB formation was systematically studied in this work. Results demonstrated that ESCs were successfully printed with hydrogel into 3D macroporous construct. Upon process optimization, about 90% ESCs remained alive after the process of bioprinting and cell-laden construct formation. ESCs continued proliferating into spheroid EBs in the hydrogel construct, while retaining the protein expression and gene expression of pluripotent markers, like octamer binding transcription factor 4, stage specific embryonic antigen 1 and Nanog. In this novel technology, EBs were formed through cell proliferation instead of aggregation, and the quantity of EBs was tuned by the initial cell density in the 3D bioprinting process. This study introduces the 3D bioprinting of ESCs into a 3D cell-laden hydrogel construct for the first time and showed the production of uniform, pluripotent, high-throughput and size-controllable EBs, which indicated strong potential in ESC large scale expansion, stem cell regulation and fabrication of tissue-like structure and drug screening studies.

With the capability of self-renewal and differentiating into all somatic cell types, embryonic stem cells (ESCs) hold great promise as an in vitro model system for studies in early embryonic development, as well as a robust cell source for applications in diagnostics, therapeutics, and drug screening [1]. Derived from the inner cell mass of a blastocyst, ESCs requires delicate culture condition and trend to cluster together, and in particular, forms three-dimensional (3D) cellular spheroids termed embryoid body (EB) [2]. In order to better understand stem cell niche and regulation of ESC differentiation and reprogramming, in vitro recapitulation of the spatial distribution of cells, cell–cell and cell–matrix interactions, is of paramount importance [3–5]. Compared with 2D monolayer culture, 3D cell culture is believed to confer a higher degree of clinical and biological relevance to in vitro model [6, 7], since the spatial arrangement of cells and extra-cellular matrix could influence cell differentiation and function both in vivo [8] and in vitro[9]. Therefore, reconstruction of 3D cell microenvironment is critical to directing stem cell fate and generating cell sources for tissue engineering, regenerative medicine and drug screening studies.

By mimicking some of the spatial and temporal aspects of in vivo development, EB is a basic 3D model for ESCs culture and differentiation studies. It was reported that the size and uniformity of EBs could vastly influence stem cell fate [10–12]. Various methods have been used to fabricate such cellular spheroid, basically including static suspension, hanging-drop and multiwell culture, most of which doesn’t involve biomaterials. Static suspension method inoculate suspension of ESCs onto non-adhesive plate to allow cells spontaneously aggregate into spheroid. This method is easy to operate, but showed limited control over the EBs size and shape due to the probability that ESCs encounter each other accidentally [13]. Hanging-drop is a common method to produce size-controlled homogeneous EBs, where droplets of ESCs suspension are pipetted onto the lid of a Petri dish and EBs was generate by gravity after overturning the dish [14]. However, manual pipetting is labor intensive and the reproducibility varies with operators. Multiwell culture offers high-throughput solution for EB formation through cell aggregation in uniformly shaped microwell arrays but requires expensive microwell culture plates [10, 15]. Besides, there are few customized microwell culture plates available in the market.

Recent advances in bioprinting technologies facilitated the precise deposition of ESCs in a reproducible manner. Xu et al [16] and Shu et al [17] printed ESCs suspension solution into 2D patterns as hanging-drop approach for EB formation, without the cell-biomaterial interaction. Corr and Xie [18, 19] applied laser direct-write method in bioprinting of mouse ESCs together with gelatin. ESCs maintained the pluripotency while proliferation and formed EB. EB size can be controlled by cell density and colony size. However, these studies just generated 2D cellular array without 3D cell–matrix interactions, and cell–cell interaction happens within one drop but not among different drops. To better recapitulate the characteristics of in vivo cell microenvironment, 3D customized cell/matrix construct with macro-porous structure might be a preferred choice. To our knowledge, there has been no report about bioprinting of ESCs into 3D cell-laden constructs.

The extrusion-based temperature-sensitive 3D bioprinting technology was developed in our lab and has been utilized for bioprinting of hepatocytes [20], adipose tissue-derived stem cells (ADSCs) [21], C2C12 cells [22], hela cells [23] and 293FT cells [24]. Most commonly used biomaterials for this technology are gelatin and alginate. Gelatin, a type of denatured collagen, is widely used as a coating for feeder layer-free mouse ES cell culture. Alginate, extracted from brown algae, is proving to have a wide applicability in tissue engineering and drug delivery and also used in embedding mouse ESCs for EB formation [25]. It has been proved in many studies that encapsulation of ESCs in hydrogels would direct EB formation with the maintenance of pluripotency [26–28]. Hence, we hypothesized that the bioprinting of 3D ESC-laden construct would maintain the stem cell pluripotency and address the challenges associated with the current methods for EB formation.

In this study, we investigated the feasibility of applying extrusion-based temperature-sensitive 3D bioprinting technology in bioprinting of ESCs with hydrogels into 3D macro-porous structure, with the maintenance of viability, pluripotency, cell growth and to direct EB formation. Printing process parameters were optimized to obtain a high cell survival rate (90%) after printing process and construct formation. Stem cell pluripotency was examined by the expression of stem cell markers (octamer binding transcription factor 4 (Oct4), stage specific embryonic antigen 1 (SSEA1) and a homeodomain-bearing transcriptional factor (Nanog)) and the ability to form EBs. The regulation of EB formation in the 3D bioprinted construct was systematically compared with commonly used methodology, where EB formation relies on cell aggregating as well as cell proliferation. Results demonstrated that this novel technology generated pluripotent, high-throughput, highly uniform and size controllable EBs under static culture condition without complex equipment. This study established the feasibility of fabricating 3D in vitro tissue-like model using ESCs for the first time, creating engineered microenvironment for pluripotent stem cells with the ability of placing cells and materials spatially in a reproducible manner.

 

Results 

3.1. 3D bioprinting and cell viability optimization

In this study, many process parameters, e.g. nozzle inner diameter, nozzle insulation temperature and chamber temperature were examined to optimize cell viability after 3D construct fabrication. It was demonstrated that larger nozzle diameter resulted in higher cell viability (figure 2(A)). Specially, the cell viability under Nozzle-160 μm (81.59% ± 1.74%) was lower than those under Nozzle-260 μm (88.06% ± 1.98%), Nozzle-410 μm (89.59% ± 0.71%) and Nozzle-510 μm (90.84% ± 1.02%), with significant differences. Nozzle diameter of 260 μm, 410 μm and 510 μm showed no significant differences in terms of cell viability.

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Figure 2. The influence of bioprinting parameters on ESC viability is determined by fluorescence live/dead staining. (A) The influence of printing nozzle inner diameter on ESC viability (Insu-30 °C and Cham-10 °C). (B) The influence of nozzle insulation temperature and chamber temperature on ESC viability. Insu-25 °C means keeping the nozzle insulation temperature at 25 °C. Cham-4 °C means setting the chamber temperature at 4 °C, and so as others. (C) The fluorescent staining images show the live (green) and dead (red) cells at different days during culture period. Scale bar: 100 μm.

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Insulation and chamber temperatures were altered to study their influences on cell viability (figure 2(B)). As a positive control, ESCs/hydrogel mixture without bioprinting were stained with fluorescence live/dead reagent, and showed 93.14% ± 1.31% cell viability. When insulation temperature was set at 25 °C (labeled as ‘Insu-25 °C’), cell viability increased with the chamber temperature from 55.52% ± 2.37% under 4 °C (labeled as ‘Cham-4 °C’) to 78.22% ± 2.55% under 10 °C (labeled as ‘Cham-10 °C’) with significant differences. When the insulation temperature was set at 30 °C (labeled as ‘Insu-30 °C’), nearly 90% ESCs remained alive under the chamber temperature of 7 °C and 10 °C (labeled as ‘Cham-7 °C’ and ‘Cham-10 °C’), significantly more than that under Cham-4 °C (72.40% ± 2.46%). To achieve both high ESC viability and a clear construct configuration, the process parameter combination of Nozzle-260 μm, Cham-10 °C and Insu-30 °C was chosen.

After culturing for three days, few cells were found dead, which were isolated from living EBs (figure 2(C)). On day 5 and day 7, a few dead cells were observed on the edge of EBs. About 5% ESCs were stained dead on day 7. As the static culturing continued, 9.69% ± 1.77%, 17.72% ± 2.91% and 40.64% ± 2.06% were found dead on day 8, day 9 and day 10, respectively (supplement 2). So, we chose 7 days as the culture period in the following analysis.

3.2. Construct structural stability and EB formation

A 3D cellular construct with the cross section of 8 mm × 8 mm and height of 1 mm was fabricated under the optimized process parameter. The 3D construct demonstrated macro-porous grid structure in which the hydrogel threads were evenly distributed according to the computer design (figure 3(A)). Both the width of the threads and the gap between the threads were homogeneous, that is 728.2 μm ± 24.9 μm and 424.3 μm ± 17.8 μm, respectively, suggesting 3D cellular construct formation in a highly controlled manner. ESCs were embedded uniformly in the hydrogel matrix threads, developing a specific 3D microenvironment.

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Figure 3. Images of the printed cellular model with grid structure. (A) Full view of the cellular construct. (B) Phase-contrast images demonstrating the cell morphology and distribution of different cell density at day 3, day 5 and day 7. Scale bar: 1 mm.

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During the culture period, ESCs tended to grow as spheroid cellular aggregates, also known as EB. The cell density in the 3D hydrogel construct were determined by the initial cell density in the ESC/alginate/gelatin mixture and showed significant influence on the yield and density of EBs formed in the construct (figure 3(B)). It was demonstrated by semi-quantitative analysis of figure 3(B) that, the percentage of area occupied by EBs varied from 52% to 85% when initial cell density changed from 0.5 mln mL−1 to 2.0 mln mL−1 . Most of the EBs were contained in the hydrogel threads in the culturing period. However, when the initial cell density was as high as 2.0 mln ml−1, some of the EBs were observed running off from the threads into the throughout holes.

3.3. Cell proliferation

ESCs formed spheroid EBs in the 3D hydrogel construct and the diameter of the EBs enlarged with culturing time while keeping their spatial location in the hydrogel thread, indicating EB formation by ESC proliferation rather than aggregation (figure 4(A)). Compared with traditional 2D culture, ESCs showed different proliferation rate indicated by the OD value measure by CCK-8 kit (figure 4(B)). The normalized OD value of the 3D in situ group grew faster than that of 2D from day 1 to day 3, while slowing down after day 3 and being much less than that of 2D at day 7. However, 3D harvest group showed a generally faster growth rate than 2D during the one week culturing, with a significant difference. In addition, the diameter of EB was also measured to indicate ESC proliferation rate. When comparing the normalized EB volume with normalized 2D OD value, 3D samples also maintained a significantly faster growth rate than 2D, though the EB volume had huge variance (figure 4(B)).

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Figure 4. EB growing and cell proliferation. (A) Magnified images of the same location in 3D printed cellular construct at different times. (B) ESC proliferation in the 3D construct compared with 2D culture. All the date were normalized to the value of day 1. Scale bar: 200 μm.

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3.4. ESCs pluripotency maintenance

Pluripotency markers, i.e. Oct4, SSEA1 and Nanog were analyzed to determine the pluripotency maintenance of ESCs after 7 day culture in the 3D hydrogel construct. Immunofluorescence staining and flow cytometry analysis showed that almost all of the cells within the EB were successfully stained both Oct4 and SSEA1. Because of the limitation of confocal capacity when dealing with large scale aggregates, the central part of the EB was darker than the edge (figure5(A)). Flow cytometry analysis demonstrated that 97.2% and 99.0% cells were positively stained with Oct4 and SSEA1 respectively (figure 5(B)). The qRT-PCR results demonstrated that the gene expression level of Oct4 and Nanog in our 3D samples were close to those in 2D (within the deviation of ±3%), without significant difference, confirming that cells have maintained pluripotency (figure 5(C)).

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Figure 5. ESC pluripotency at day 7 was determined by CLSM, flow cytometry and qRT-PCR. (A) Immunofluorescence images of EBs stained with Oct4, SSEA1 and DAPI. (B) Quantification of 3D dissociated cells marked with Oct4 and SSEA1 by using flow cytometry. (C) Gene expression of Oct4 and Nanog in 3D versus 2D by using qRT-PCR. Scale bar: 50 μm.

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3.5. EB morphology

EBs were harvested from the 3D hydrogel construct at different time intervals to analyze EB morphology (figure 6(A)). Most of the EBs were separated without fusion. The center part of the EBs was darker than edge part, especially at day 5 and day 7, indicating the 3D sphere structure of EBs. Through analyzing the size of 250 random EBs for each sample, the histogram of EB diameter were obtained, showing a Gauss distribution curve (figure 6(B)). The results demonstrated that the EB size increased significantly from about 50 μm to about 110 μm when the construct was cultured from day 3 to day 7 (figure 6(C)). Cell density had little influence on EB average size. However, increased cell density would result in the reduction of the uniformity of EB size, especially at day 7; the EB diameter of 2.0 mln mL−1 group at day 7 was vastly heterogeneous, with a deviation of 42.30 μm, which was much more than those of other two groups.

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Figure 6. EB formation in different cell density: (A) optical images of released EBs at different days. (B) EB diameter and (C) EB circularity distributions at different days. Summary of the (D) diameter and (E) circularity. 250 EBs were applied for diameter and circularity measurements for each group. Scale bar: 200 μm.

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Circularity was measured to assess the quality of EBs (figure 6(D)). For the 0.5 mln mL−1 group, most of the EBs were close to a standard spheroid with the circularity centered in 0.9 for the three time points. As to the other two groups, the circularity at day 3 is similar to that of 0.5 mln mL−1group, while the circularity frequency peaks had a significant decrease at day 5 and day 7. In particular, about 20% EBs had a circularity under 0.8 at day 5 and day 7 for the 2.0 mln mL−1group. In general, the circularity decreased with the increase of culture time and initial cell density in the hydrogel (figure 6(E)).

3.6. Comparison with other EB formation methods

Considering this was a novel methodology of EB formation, we systematically compared the commonly used static suspension and hanging drop methods with the 3D bioprinting method for EB formation. As demonstrated by the phase-contrast images (figure 7(A)), EBs generated by static suspension method showed more uncontrollable morphology rather than round spheroid. The distribution of EB diameter clearly demonstrated that 3D bioprinting technology generated EBs with higher uniformity compared with static suspension technology, especially for the larger EB diameter, i.e. 60 ~ 70 μm and 100 ~ 110 μm regions (figure 7(B)). In particular, the EBs with 30 ~ 50 μm diameter presented vastly irregular shape in suspension technology, which was confirmed by the circularity curve (figure 7(C)). On the other hand, EBs generated by 3D bioprinting technology showed higher circularity regardless of the diameter regions, suggesting more regular shape (figure 7(C)). More characteristic like EB forming motivation, size control method, EB diameter range, uniformity, yield, operation complexity were compared among 3D bioprinting technology, static suspension technology and hanging drop technology, as listed in table 1.

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Figure 7. Comparison of static suspension and 3D bioprinting technology for generating EBs. (A) Phase-contrast images showing the morphology of EBs generated by static suspension technology and 3D bioprinting technology. (B) The EB diameter histograms presented the distribution of EB size with a Gauss distribution fitting. (C) The circularity curves contrasted the EB qualities.

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Table 1.  Comparison of three EB forming methods.
Hanging-drop Suspension 3D print
Forming mechanism Aggregation by gravity Self-aggregation Proliferation
Size control Time and cell density Time and cell density Mainly time
Diameter range 50 ~ 500 μm 50 ~ 500 μm 30 ~ 200 μm
Uniformity High Low Medium-high
Yield Low High High
Operation Time-consuming for seeding and medium refresh Complex for medium refresh Time-saving and easy for medium refresh

 

4. Discussion

3D cell culture environment and tissue-like models have drawn great attention because they can be tuned to promote certain levels of cell differentiation and tissue organization, which is difficult in traditional 2D culture systems for their failing to reconstitute the in vivo cellular microenvironment [30, 31]. Various 3D culture systems have been developed to study the cellular behavior affected by spatial and temporal cell–cell and cell–matrix interactions. Among these methods, 3D bioprinting, typically containing jet-, laser- and extrusion-based methods, is a promising technique to manipulate cells/matrix deposition and ultimately generate 3D complex tissues or organs. This technique have been used in printing cells derived from adult, embryonic and even tumor tissues for tissue engineering and drug screening applications. With the capacity to expand unlimitedly in vitro and differentiate into a variety of therapeutic cell types, ESCs have generated great enthusiasm and are being applied in bioprinting studies until recently. As a relatively sensitive cell type, ESCs might suffer greater problems in a printing process compared with other types of cells. Several studies had been conducted to print ESCs, maintaining their viability and pluripotency [16–19]. Instead of creating 3D tissue-like constructs, these studies were more likely to generate cellular droplet array with precise control of distribution. Here we described the work of establishing a 3D ESC-laden hydrogel construct using extrusion-based bioprinting technology. The results demonstrated high proliferation rate of pluripotent ESCs in the hydrogel construct, and a versatile technology for generating highly uniform and high throughput EBs.

Cell viability after 3D bioprinting and construct formation was determined when evaluating the limitations of bioprinting ESCs. Cells would be lysed or damaged due to osmotic effects in the solution, heat increase and mechanical stress during printing. In the protocol presented in this work, about 6.86% ± 1.31% cells were dead during the cell/hydrogel solution preparation process before 3D bioprinting (figure 2(B)). We assumed this was caused by cell dissociation process, together with the osmosis and stirring operation of hydrogel materials. In an inkjet printing study, 15% Chinese Hamster Ovary cells were detected dead before printing process [32]. Thermal effects of the ejector reservoir in the inkjet printing process and laser force in laser-based printing would be the cause of cell death, in addition to the impact force when cellular droplets were jetted to a rigid substrate in a very short time. Under a different fabricating strategy, the extrusion-based bioprinter extruded the cell-laden cylinders softly on the substrate and controlled the temperature under 30 °C, without the concerns about the thermal and sharply impacting effects. However, cells would inevitably suffer from shear force when the cell-laden hydrogels were continuously extruded through a limited space in the nozzle. We hypothesized that nozzle size and hydrogel viscosity would influence shear force and hence influence cell viability. The cell viability data of different nozzle sizes, chamber and insulation temperatures supported this hypothesis (figures 3(A) and (B)). In our previous study, more than 90% Hela cells were alive after bioprinting under the parameters of Insu-25 °C/Cham-4 °C and Nozzle-260 μm [23], while the viability of ESCs was only 55.52% ± 2.37% under the same parameter combination. When increasing the insulation and chamber temperature to 30 °C and 10 °C respectively, the viability showed a significant increase to 90%. Taking into the account of cell death before bioprinting, optimized parameters led to only 5% cell death during printing, indicating a broad future applicability of this technique to various cell types ranging from tumor cells to ESCs. Additionally, few dead cells were observed during one-week culture period (figure3(C)). On the other hand, when the culture period was extended to more than 7 days, more and more ESCs suffered from apoptosis and lysis, possibly due to contact inhabitation and insufficient mass transfer to the center of EB with the increasing of EB size. Therefore, 7 days was chose as the experiment time window for this study.

Apart from cell viability, the maintenance of pluripotency is another essential criterion for ESCs regulation and application. The results of immunofluorescence staining and FACS analysis showed a high expression rate (98%) of stem cell pluripotent markers Oct4 and SSEA1 at day 7 (figure 4), indicating that cells remained undifferentiated state during the whole experimental period. Naturally, it can be inferred that the printing process also had little influence on ESC pluripotency.

In the cell-laden hydrogel culture system, both the cell type and matrix material could influence cell growth. Human mesenchymal stem cells remained alive but did not proliferate when encapsulated in alginate [33, 34]. While human ADSCs could proliferated for a short period of time in alginate hydrogel microspheres but showed significantly higher proliferation rate in gelatin/alginate microspheres [35]. As a widely used hydrogel, alginate has the disadvantages of low cell adhesiveness and poor support for cell proliferation [36]. Adding gelatin would improve the cellular adhesive condition and hence favor cell expansion. In this study, the fabricated multilayered constructs offered a 3D microenvironment surrounded by gelatin/alginate materials for ESCs to adhere, self-renew, and cellular spheroid, termed EB, was generated in situ because of cell proliferation. Once EB was formed, the spheroid structure supported expansion of subpopulations with differing proliferation, nutrition and oxygenation status compared with conventional monolayer system. It is reported that the proliferation of mouse ESCs was higher when embedded in fibrin gels versus 2D suspension culture [27]. Similarly, in this study, ESCs in 3D constructs proliferated faster than 2D culture sample when being released from hydrogel to read OD value. This operation was aimed to avoid the influence of interactions between reagent molecular and matrix materials (figure 6 and supplement 3). Additionally, the enlargement of EB diameter, which also reflected ESC proliferation, confirmed this result (figure 6).

Typically stimulated via generation of EBs, ESC differentiation depends on numerous cues throughout the EB environment, including EB size and shape, as well as their uniformities. In general, several characteristics should be concerned for EB formation system, including reproducibility, symmetry, ease of use and scalability [37]. In the traditional EB formation methodology, like suspension and hanging-drop, EBs were created via cell gathering and proliferation. In these methods, it was essential to get a balance between allowing necessary ESC aggregation for EB formation and preventing EB agglomeration for efficient cell growth and differentiation [14]. Static suspension cultures produced a large number of EBs with simple operation, but the size and shape of the resulting EBs were highly uncontrollable and irregular due to the tendency of EBs to agglomerate after initial formation, as shown in figure 7. Hanging-drop method served as a golden tool to generate uniform and reproducible EBs with fully aggregating of cells under gravity and non-agglomeration of EBs in different drops. However, it faced the intrinsic limitation of scalability. The 3D bioprinting method presented in this study addressed some of the problems, producing massively homogeneous EBs with regular shape and controllable shape. In this 3D cell-laden hydrogel system, ESCs were immobilized and restricted to aggregate with each other, and would not agglomerate until they are large enough to connect with each other. When the initial cell density was increased, the average distance between two original EBs was closer and these EBs are more likely to agglomerate with each other while proliferation, which is also one of the concerns when we choose the experiment time period. As a result, the EB uniformity of 2.0 mln mL−1 group was not that good as those of 0.5 mln mL−1 and 1.0 mln mL−1 groups, especially after culturing for one week (figure 6). Without the initial cell aggregating, the size of EBs in our model was mainly determined by the culture time. Also, it would take longer to reach the same scale of EB diameter compared with suspension method, probably due to the physical constrain of the matrix material. For example, it took 5 days and 2 days to get EBs ranging 60 ~ 70 μm for 3D printing and suspension methods, respectively (figure7). Besides, thanks to the interconnected channels design in the 3D construct which allowed mass transfer, EBs could be produced in a large scale by changing the construct volume and cell density. In the six-layer construct with 1.0 million cells per milliliter for example, EBs got a stable yield of about 3000 cm−2, while the EB yield by suspension technology was about 900 cm−2(seeding 0.5 million cells in a 35 mm dish) and no more than 10 EBs [38] could be produced in 1 cm2 area in hanging-drop method, which was also demonstrated by our experiments (supplement 4).

In summary, this study presented the high throughput production of pluripotent, uniform, regular and controllable EBs with the diameter smaller than 150 μm during one week culture. In a gelatin-based laser printing method, EBs with the diameter of about 100 μm were also generated to avoid EB agglomeration in gels [19]. EBs with different size exhibit different gene expression and differentiation fate. Park et al [39] found that 100 μm diameter EBs of mouse ESCs expressed increased ectoderm markers while 500 μm diameter EBs expressed endoderm and mesoderm markers. Furthermore, Messana et al [12] demonstrated that mouse ESCs derived from small EBs (<100 μm) had a greater chondrogenic potential than those from larger EBs. Hwang [10] reported that human endothelial cell differentiation was increased in smaller EBs (150 μm) while cardiogenesis was enhanced in larger EBs (450 μm). However, large EBs might be associated with limited mass transfer and the diffusion of biochemical through EBs is demonstrated to be linked to differentiation of ESCs [40]. While the effect of EB size on differentiation remains to be shown in our model, we hypothesize that EBs with the diameter smaller than 150 μm would mediate specific differentiation trajectory, which will be confirmed in the future work.

Demonstrating the advantages of reproducibility, high throughput, regular shape and controlled size, we believe this is a versatile technology for EB generation. But, this 3D printing system does not serve as an EB formation method solely. The ESC-laden hydrogel 3D construct can be dissolved at a proper time point to harvest massive EBs with desired size for ES cell research. Or, the ESC-laden hydrogel 3D construct can be maintained to perform 3D ESC differentiation studies to explore the regulation of EB size, matrix material and 3D structure on ESC differentiation lineages. Furthermore, this technology hold the potential to serve as a versatile tool for the generation of tissue-like structure and organ/tissue on chip based on controlled ESC differentiation.

5. Conclusion

In this study, we reported successful bioprinting of mouse ESCs with hydrogel into a 3D multilayered construct for the first time. Extrusion-based bioprinting technology was applied. Upon parameter optimization, ESCs demonstrated high viability of 90% after 3D printing and construct formation. Cells continued self-renewal in the construct and exhibited a higher proliferation rate compared with conventional 2D culture. 98% cells expressed the canonical pulripotent markers Oct4 and SSEA1 at day 7, indicating that most of the ESCs remained undifferentiated state after printing and culturing. Large quantities of uniform EBs with regular shape and adjustable size were generated through cell proliferation, while avoiding EBs agglomeration. This work indicated the feasibility of fabricating complex 3D tissue-like model based on pluripotent stem cells for applications in pharmacy, regenerative medicine, stem cell expansion and biology studies.

 

Bioprinting of human pluripotent stem cells and their directed differentiation into hepatocyte-like cells for the generation of mini-livers in 3D

Alan Faulkner-Jones1,2, Catherine Fyfe3, Dirk-Jan Cornelissen1,2, John Gardner3, Jason King3,4,Aidan Courtney3,4 and Wenmiao Shu1,2
http://iopscience.iop.org/article/10.1088/1758-5090/7/4/044102/meta

We report the first investigation into the bioprinting of human induced pluripotent stem cells (hiPSCs), their response to a valve-based printing process as well as their post-printing differentiation into hepatocyte-like cells (HLCs). HLCs differentiated from both hiPSCs and human embryonic stem cells (hESCs) sources were bioprinted and examined for the presence of hepatic markers to further validate the compatibility of the valve-based bioprinting process with fragile cell transfer. Examined cells were positive for nuclear factor 4 alpha and were demonstrated to secrete albumin and have morphology that was also found to be similar to that of hepatocytes. Both hESC and hiPSC lines were tested for post-printing viability and pluripotency and were found to have negligible difference in terms of viability and pluripotency between the printed and non-printed cells. hESC-derived HLCs were 3D printed using alginate hydrogel matrix and tested for viability and albumin secretion during the remaining differentiation and were found to be hepatic in nature. 3D printed with 40-layer of HLC-containing alginate structures reached peak albumin secretion at day 21 of the differentiation protocol. This work demonstrates that the valve-based printing process is gentle enough to print human pluripotent stem cells (hPSCs) (both hESCs and hiPSCs) while either maintaining their pluripotency or directing their differentiation into specific lineages. The ability to bioprint hPSCs will pave the way for producing organs or tissues on demand from patient specific cells which could be used for animal-free drug development and personalized medicine.

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Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.

 

New drug development can take 10 to 20 years with an estimated average of about 9 to 12 years [1, 2]. In addition, only around 16% of the drugs that begin preclinical testing are approved for human use [3]. Some of this low success rate can be attributed to the different responses that animals and humans have to the drugs being tested; some drugs have to be withdrawn from market due to toxic effects on human organs such as liver and heart, despite being tested safely on animals. A possible solution to this might be the creation of human pluripotent stem cell (hPSC) -derived micro-tissues which could be used with organ-on-a-chip devices [4–7]. These micro-tissues are expected to produce the same or similar physiological reaction that the entire organ would but on a much smaller scale. This would result in scalable, faster and potentially more reliable drug testing platform, and hopefully an end to animal testing.

hPSCs are the ideal cells to use for this application due to their ability to self-renew indefinitely, which enables large populations of cells to be created easily in vitro, and their pluripotency which means that they can be differentiated into any required adult cell type [8–13]. Pluripotent stem cells can be divided into embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Human ESCs (hESCs) were first isolated from early human blastocysts in 1998 [14]. Any tissue construct created from hESCs for implantation in vivo would require the patient to receive immunosuppressive drugs and ethical issues still restrict some applications due to their source. iPSCs have neither of these drawbacks as they can be created from harvested adult cells from the patient requiring treatment and as such any implanted cells derived from these iPSCs should not be rejected by the patient’s immune system but may require immunosuppressive drugs at a greatly reduced dosage. In 2006 Shinya Yamanaka discovered that iPSCs can be derived from somatic cells by retrovirally transducing them with four transcription factors—Oct3/4, Sox2, Klf4 and C-myc [15, 16]. These cells have the same self-renewal and differentiation capabilities as ESCs but with the added advantage that iPSCs can be used for autologous therapies. These unique characteristics make pluripotent stem cells ideal for use in a number of applications such as clinical tissue engineering, novel drug discovery and testing for the pharmaceutical industry [8,9, 17, 18].

In the field of biofabrication, great advances are being made towards fabricating 3D tissue and organs with very fine spatial control of cell deposition. From the very first paper that was published investigating printing of biological cells (or bioprinting), tissue engineering was identified as a major application for this new technology [19]. If more complex structures such as organs and tissues were to be printed, the bioprinter would need the ability to transfer microscopic patterns of viable cells of multiple cell types into well-defined three-dimensional arrays that closely mimic the tissue structure. There has been much progress in the development and establishment of several different bioprinting techniques for 3D live constructs [20–22] including those based on laser pulses, inkjets and other more novel approaches. It is an inescapable fact that cells will be subjected to some level of stress during deposition, regardless of the printing technique being used. For example, cells printed by non-contact methods will be affected when they impact on the substrate at some incident velocity, which would result in extreme deceleration and shear stress [23–26]. Shear stress is applied to cells pushed through nozzle orifices and capillary tubes [24, 27–42] and the actuation is provided via pressure, heat, or high frequency vibration which can also be damaging to the cells [30, 31, 43–46]. If cells are exposed to laser energy the radiation can cause genetic damage [29, 47–54] and shear forces are applied during cavitation and jet formation [23, 55]. Ultrasonic actuation for cell transfer would subject the cells to stress in the form of heat and vibration [56, 57]. Therefore, it is important to validate the response of printed cells to any particular bioprinting process in terms of their viability and more importantly their biological functions.

We previously reported the results of the first experiments printing hESCs using a valve-based printing approach including their response to the printing process in the form of post-printed viability and pluripotency validation [37]. However, if hPSCs are to be used for producing human tissues on demand for drug testing, their post-printing differentiation must be reproducibly directed to the required lineages for each tissue. Unfortunately homogenous cellular differentiation of hPSCs into some germ layers has proved difficult [12, 13]. Here, we report the first investigation into the bioprinting of human iPSCs, their response to the valve-based printing process as well as their post-printing differentiation into hepatocyte-like cells (HLCs). HLCs that are in the process of differentiating are bioprinted and examined to further validate the compatibility of the valve-based bioprinting process with fragile cell transfer. Finally, 3D hydrogel structures were designed and printed out with encapsulated hESC-derived HLCs and the viability and hepatic characteristics of the cells were investigated.

 

A newer version of our previously reported cell printing platform [37] has been developed. Four nanolitre dispensing systems, each comprising a solenoid valve (VHS Nanolitre Dispense Valve, Lee Products Ltd) with 101.6 μm internal diameter nozzles (Minstac Nozzle, Lee Products Ltd), were attached to static pressure reservoirs for the bio-ink solution to be dispensed from via flexible tubing. The nanolitre dispensing system and bio-ink reservoirs were mounted onto the tool head of an enclosed custom built micrometer-resolution 3-axis XY–Z stage (figure 1). This newer cell printing platform improved on the previous version by reducing the overall size and weight of the machine, allowing it to be mounted inside a standard tissue culture hood during experiments requiring a sterile environment. Other enhancements included the two extra nanolitre dispensing systems, taking the total up to four, a more robust electronics and custom firmware was developed which improved the reliability and speed of the machine and two separate pressure channels were included, allowing for differential bio-ink dispensing conditions. Unless otherwise stated standard printing conditions were used: for 2D, printing was carried out using a pulse time of 8 ms at an inlet pressure of 0.6 bar using a nozzle with an internal diameter of 101.6 μm; for 3D, printing was carried out using a pulse time of 400 μs at an inlet pressure of 1.0 bar for sodium alginate solution and a pulse time of 400 μs at an inlet pressure of 0.5 bar for calcium chloride solution both using nozzles with an internal diameter of 101.6 μm.

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Figure 1. (a) Schematic drawing of the cell printer system; (b) detailed schematic of the micro-solenoid valve; (c) schematic of the combinatorial printing process for alginate hydrogel creation; (d) a 3D printed alginate tube structure approximately 13 mm tall printed with 1.5% w/v Sodium Alginate and 600 mM (6%) Calcium Chloride solutions in Millipore water (scale bar 2 mm).

 

……

The process of in vivo liver organogenesis occurs in the developing foregut, when newly specified hepatic cells separate from the endodermal sheet and form a dense 3D structure known as a hepatoblast (liver bud) [74, 75]. It is hypothesized that arranging the hESC–HLCs in 3D during the differentiation process may yield more mature hepatocytes than conventional 2D differentiation. The hESC differentiation protocols are more efficient and robust than hiPSC protocols therefore only hESC-derived HLCs were printed in 3D.

In order for this technique to be useful for tissue engineering applications, structures need to be tall enough to allow cells to interact in a three-dimensional environment. The concentration of alginate solution was set to 1.5% w/v to improve the mechanical strength of the hydrogel and allow it to support further layers. Circular structures with a large number of layers were designed and printed out in the wells of a multi-well plate to allow the structures to be cultured post-printing. These resulting structures were photographed for analysis and are shown in figure 7below.

These structures were printed out in a matter of minutes and are strong enough to support their own weight and the weight of further layers (as seen in figure 1(d)). The structures spread slightly, but by slightly altering the volume ratio, concentrations and surface properties this spreading can be reduced.

Approximately one hour post-printing one of the HLC-laden alginate ring structures was examined using a confocal microscope; the 3D image is shown in figure 8(a). Cell viability was calculated to be 55.5% using the Imaris confocal microscope software. Cell viability declined over the first 24 h which resulted in low cell numbers for hepatic marker testing following the 3D differentiation process, but the viability remained stable for the remainder of the differentiation process. At day 23 of the differentiation process, the cells in the remaining structures were harvested and stained for the presence of hepatic markers. As shown in figure 8(b), cells are positive for albumin which demonstrates their hepatic lineage. The normal time required for 2D differentiation of hPSC-HLCs is 17–24 d. However, based on the results of albumin secretion in the medium, we observed the 3D printed cells have taken longer to reach the maximum albumin secretion than the 2D control as shown in figure 8(c). Interestingly, when analyzing the difference between 20 and 40 layer printed tube structures, we noticed close-to proportional increase in albumin secretion to the number of layers as shown in figure 8(d). This indicates that the permeability of the alginate hydrogel allows nutrition and differentiation reagents to enter the structure and support 3D differentiation and maturation processes of the cells, regardless of the height of the printed structure.

Research is currently underway including investigations to improve the 3D viability and adjusting the differentiation protocol that may facilitate higher albumin secretion. For example, the optimization of hydrogel formation as well as enhanced cell density may improve the differentiation process for hPSCs in 3D [21, 76, 77].

4. Conclusions

To the best of our knowledge, this study is the first to demonstrate that hiPS cells can be bioprinted without adversely affecting their biological functions including viability and pluripotency. Importantly, we verified that our valve-based printing process is gentle enough to not affect the pluripotency of both hESCs and hiPSCs. A number of different hPSC lines were directed to differentiate into HLCs. Cells were printed during the differentiation process and showed no differences in hepatocyte marker expression and similar morphology when compared to a non-printed control. We previously reported the results of an investigation into the response of hESCs to the valve-based printing process. Here we build on that study, performing a deeper investigation to compare the response of hiPSCs and hESCs to the printing process using flow cytometry. The effect of nozzle geometry was investigated and the effects of nozzle length on the post-printing viability of cells were recorded; longer nozzles lower the post-printing viability of the cells. We printed hESC-derived HLCs in a 3D alginate matrix and tested for viability and hepatic markers during the remaining differentiation and they were found to be hepatic in nature. The ability to bioprint hPSCs while either maintaining their pluripotency or directing their differentiation into specific cell types will pave the way for producing organs or tissues on demand from patient specific cells which could be used for animal-free drug development and personalized medicine.

 

 

Large scale industrialized cell expansion: producing the critical raw material for biofabrication processes

Arun Kumar1 and Binil Starly1,2

http://iopscience.iop.org/article/10.1088/1758-5090/7/4/044103

Cellular biomanufacturing technologies are a critical link to the successful application of cell and scaffold based regenerative therapies, organs-on-chip devices, disease models and any products with living cells contained in them. How do we achieve production level quantities of the key ingredient—’the living cells‘ for all biofabrication processes, including bioprinting and biopatterning? We review key cell expansion based bioreactor operating principles and how 3D culture will play an important role in achieving production quantities of billions to even trillions of anchorage dependent cells. Furthermore, we highlight some of the challenges in the field of cellular biomanufacturing that must be addressed to achieve desired cellular yields while adhering to the key pillars of good manufacturing practices—safety, purity, stability, potency and identity. Biofabrication technologies are uniquely positioned to provide improved 3D culture surfaces for the industrialized production of living cells.

Biofabrication of tissue constructs by 3D bioprinting of cell-laden microcarriers

Riccardo Levato1,2, Jetze Visser3, Josep A Planell1, Elisabeth Engel1,2,4, Jos Malda3,5 andMiguel A Mateos-Timoneda2,1

http://iopscience.iop.org/article/10.1088/1758-5082/6/3/035020

Bioprinting allows the fabrication of living constructs with custom-made architectures by spatially controlled deposition of multiple bioinks. This is important for the generation of tissue, such as osteochondral tissue, which displays a zonal composition in the cartilage domain supported by the underlying subchondral bone. Challenges in fabricating functional grafts of clinically relevant size include the incorporation of cues to guide specific cell differentiation and the generation of sufficient cells, which is hard to obtain with conventional cell culture techniques. A novel strategy to address these demands is to combine bioprinting with microcarrier technology. This technology allows for the extensive expansion of cells, while they form multi-cellular aggregates, and their phenotype can be controlled. In this work, living constructs were fabricated via bioprinting of cell-laden microcarriers. Mesenchymal stromal cell (MSC)-laden polylactic acid microcarriers, obtained via static culture or spinner flask expansion, were encapsulated in gelatin methacrylamide-gellan gum bioinks, and the printability of the composite material was studied. This bioprinting approach allowed for the fabrication of constructs with high cell concentration and viability. Microcarrier encapsulation improved the compressive modulus of the hydrogel constructs, facilitated cell adhesion, and supported osteogenic differentiation and bone matrix deposition by MSCs. Bilayered osteochondral models were fabricated using microcarrier-laden bioink for the bone compartment. These findings underscore the potential of this new microcarrier-based biofabrication approach for bone and osteochondral constructs.

Microstereolithography and characterization of poly(propylene fumarate)-based drug-loaded microneedle arrays

Yanfeng Lu1, Satya Nymisha Mantha1, Douglas C Crowder2, Sofia Chinchilla2, Kush N Shah2,3,4,Yang H Yun2, Ryan B Wicker5 and Jae-Won Choi1

http://iopscience.iop.org/article/10.1088/1758-5090/7/4/045001/meta

Drug-loaded microneedle arrays for transdermal delivery of a chemotherapeutic drug were fabricated using multi-material microstereolithography (μSL). These arrays consisted of twenty-five poly(propylene fumarate) (PPF) microneedles, which were precisely orientated on the same polymeric substrate. To control the viscosity and improve the mechanical properties of the PPF, diethyl fumarate (DEF) was mixed with the polymer. Dacarbazine, which is widely used for skin cancer, was uniformly blended into the PPF/DEF solution prior to crosslinking. Each microneedle has a cylindrical base with a height of 700 μm and a conical tip with a height of 300μm. Compression test results and characterization of the elastic moduli of the PPF/DEF (50:50) and PPF/drug mixtures indicated that the failure force was much larger than the theoretical skin insertion force. The release kinetics showed that dacarbazine can be released at a controlled rate for five weeks. The results demonstrated that the PPF-based drug-loaded microneedles are a potential method to treat skin carcinomas. In addition, μSL is an attractive manufacturing technique for biomedical applications, especially for micron-scale manufacturing.

Controlling shape and position of vascular formation in engineered tissues by arbitrary assembly of endothelial cells

Hiroaki Takehara1,4, Katsuhisa Sakaguchi2, Masatoshi Kuroda3, Megumi Muraoka3, Kazuyoshi Itoga1,Teruo Okano1 and Tatsuya Shimizu1
http://iopscience.iop.org/article/10.1088/1758-5090/7/4/045006/meta

 

Cellular self-assembly based on cell-to-cell communication is a well-known tissue organizing process in living bodies. Hence, integrating cellular self-assembly processes into tissue engineering is a promising approach to fabricate well-organized functional tissues. In this research, we investigated the capability of endothelial cells (ECs) to control shape and position of vascular formation using arbitral-assembling techniques in three-dimensional engineered tissues. To quantify the degree of migration of ECs in endothelial network formation, image correlation analysis was conducted. Positive correlation between the original positions of arbitrarily assembled ECs and the positions of formed endothelial networks indicated the potential for controlling shape and position of vascular formations in engineered tissues. To demonstrate the feasibility of controlling vascular formations, engineered tissues with vascular networks in triangle and circle patterns were made. The technique reported here employs cellular self-assembly for tissue engineering and is expected to provide fundamental beneficial methods to supply various functional tissues for drug screening and regenerative medicine.

The influence of printing parameters on cell survival rate and printability in microextrusion-based 3D cell printing technology

Yu Zhao1,2, Yang Li1,2, Shuangshuang Mao1,2, Wei Sun1,2,3,4 and Rui Yao1,2
http://iopscience.iop.org/article/10.1088/1758-5090/7/4/045002/meta

Three-dimensional (3D) cell printing technology has provided a versatile methodology to fabricate cell-laden tissue-like constructs and in vitro tissue/pathological models for tissue engineering, drug testing and screening applications. However, it still remains a challenge to print bioinks with high viscoelasticity to achieve long-term stable structure and maintain high cell survival rate after printing at the same time. In this study, we systematically investigated the influence of 3D cell printing parameters, i.e. composition and concentration of bioink, holding temperature and holding time, on the printability and cell survival rate in microextrusion-based 3D cell printing technology. Rheological measurements were utilized to characterize the viscoelasticity of gelatin-based bioinks. Results demonstrated that the bioink viscoelasticity was increased when increasing the bioink concentration, increasing holding time and decreasing holding temperature below gelation temperature. The decline of cell survival rate after 3D cell printing process was observed when increasing the viscoelasticity of the gelatin-based bioinks. However, different process parameter combinations would result in the similar rheological characteristics and thus showed similar cell survival rate after 3D bioprinting process. On the other hand, bioink viscoelasticity should also reach a certain point to ensure good printability and shape fidelity. At last, we proposed a protocol for 3D bioprinting of temperature-sensitive gelatin-based hydrogel bioinks with both high cell survival rate and good printability. This research would be useful for biofabrication researchers to adjust the 3D bioprinting process parameters quickly and as a referable template for designing new bioinks.

A new method of fabricating a blend scaffold using an indirect three-dimensional printing technique

Jin Woo Jung1,3, Hyungseok Lee1,3, Jung Min Hong1, Jeong Hun Park1, Jung Hee Shim2, Tae Hyun Choi2and Dong-Woo Cho1
http://iopscience.iop.org/article/10.1088/1758-5090/7/4/045003/meta

Due to its simplicity and effectiveness, the physical blending of polymers is considered to be a practical strategy for developing a versatile scaffold having desirable mechanical and biochemical properties. In the present work, an indirect three-dimensional (i3D) printing technique was proposed to fabricate a 3D free-form scaffold using a blend of immiscible materials, such as polycaprolactone (PCL) and gelatin. The i3D printing technique includes 3D printing of a mold and a sacrificial molding process. PCL/chloroform and gelatin/water were physically mixed to prepare the blend solution, which was subsequently injected into the cavity of a 3D printed mold. After solvent removal and gelatin cross-linking, the mold was dissolved to obtain a PCL–gelatin (PG) scaffold, with a specific 3D structure. Scanning electron microscopy and Fourier transform infrared spectroscopy analysis indicated that PCL masses and gelatin fibers in the PG scaffold homogenously coexisted without chemical bonding. Compression tests confirmed that gelatin incorporation into the PCL enhanced its mechanical flexibility and softness, to the point of being suitable for soft-tissue engineering, as opposed to pure PCL. Human adipose-derived stem cells, cultured on a PG scaffold, exhibited enhanced in vitro chondrogenic differentiation and tissue formation, compared with those on a PCL scaffold. The i3D printing technique can be used to blend a variety of materials, facilitating 3D scaffold fabrication for specific tissue regeneration. Furthermore, this convenient and versatile technique may lead to wider application of 3D printing in tissue engineering.

 

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Twitter Analytics on the Inside 3DPrinting Conference #I3DPConf

Posted in 3D Plotting Scaffolds, 3D Printing for Medical Application, Conference Coverage with Social Media, Curation, tagged , , , on October 25, 2015| Leave a Comment »

Twitter Analytics on the Inside 3DPrinting Conference #I3DPConf

Reporter & Curator: Stephen J. Williams, Ph.D.

 

The following is a Twitonomy Analysis of Tweets from the Inside 3D Printing Conference

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Top hashtags for Conference #I3DPConf
#i3dpconf1132 #3dprinting515 #startupcompetition49 #3dpweek47 #3dprint32 #3dprinted31 #design30 #metamason30 #vr28 #startup27

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1,132 tweetsfrom Oct. 16, 4:10 am to Oct. 25, 1:14 pm1 Download & Print
#I3DPConf16. Oct17. Oct18. Oct19. Oct20. Oct21. Oct22. Oct23. Oct24. Oct25. Oct0100200300400
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329 users Potential Reach 5,919,064
Most influential users
74,839 69,582 54,848 50,066 46,499
41,815 40,617 33,757 32,354 26,715
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257 99 77 67 60
51 48 44 40 32
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@Inside3DPrint
Inside3DPrint 4,256 3,143 221 203 mentions
122 retweets 135 favorites
@3DPrint_com
3DPrint 12,239 33,757 297 36 mentions
22 retweets 38 favorites
@brianfederal
Brian Federal 13,480 10,290 251 31 mentions
44 retweets 33 favorites
@fabbing
alex@fabbing 43,597 8,565 486 26 mentions
@bazooka_tech
Bazooka Tech 38,000 1,173 732 23 mentions
@SJGrunewald
la moustache ringard 1,330 234 38 23 mentions
18 retweets 33 favorites
@rapidprototypin
Rapid Prototyping 106,464 3,396 1,104 18 mentions
@TheUPSStore
The UPS Store 11,758 11,688 336 17 mentions
21 retweets 46 favorites
@aaronilevinson
Aaron Levinson 160 57 2 17 mentions
5 retweets 13 favorites
@RealityInc
Reality™ Institute 7,745 534 58 16 mentions
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Tweets Download & Print
printhuman @printhuman1:14 PM – 25 Oct 2015 to 26,715 followers 4 0

RT @brianfederal: The Future of Construction will be 3D Printed. Tony Capasso Inside 3D Printing #3DPrinting #I3DPConf https://t.co/f77wzUp…

Brian Federal @brianfederal1:07 PM – 25 Oct 2015 to 10,290 followers 2 0

RT @PixelxVore: Here at #I3DPConf for #3DPWeek in Santa Clara with @TheUPSStore’s Ask The Experts panel. https://t.co/9Nh60vcADP
3DMaterialsExpert @djamilaolivier11:33 PM – 24 Oct 2015 to 504 followers 8 0

RT @3DPrintBoard: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https:/…

The Original Mika @muehara6:50 PM – 24 Oct 2015 to 287 followers 4 0
RT @sffashiontech: Customization is a loaded term. True custom requires scans, body modeling, vertex matching, more. We’re just scratching …
VR News Rover @VRNewsRover3:07 PM – 24 Oct 2015 to 644 followers 1 0

RT @Inside3DPrint: “In 10 years, we will not take a #vacation without being there virtually first” @SivanIram @RiverVR @rothventures #virtu…

be connect3D @beconnect3D2:36 PM – 24 Oct 2015 to 1,338 followers 7 0
RT @3DPrintGirl: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t.…
James Walton @JAWaltonLBGMfg10:50 AM – 24 Oct 2015 to 166 followers 5 0

RT @3DPrintGirl: The Medical Track offers incredible sessions #3Dprinting sessions at #I3DPConf next week! 3dprint.com/100840/medical… http://t.…

Sathyan Nagarajan @SathyanBN8:36 AM – 24 Oct 2015 to 58 followers 10 0

RT @3DPrintGirl: Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/WPhiwz…

Laurence Gartel @GARTEL5:29 AM – 24 Oct 2015 to 558 followers 0 2

#I3DPConf #MecklerMedia put on a phenomenal thought provoking Expo. #CreativeForce @GARTEL https://t.co/LAdJemzqba

Jorma Tevajärvi @JormaTevajrvi3:03 AM – 24 Oct 2015 to 1,770 followers 5 0

RT @3DPrintGirl: The Medical Track offers incredible sessions #3Dprinting sessions at #I3DPConf next week! 3dprint.com/100840/medical… http://t.…

Jorma Tevajärvi @JormaTevajrvi3:01 AM – 24 Oct 2015 to 1,770 followers 4 0

RT @3DPrintGirl: “Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.c…

Jorma Tevajärvi @JormaTevajrvi3:00 AM – 24 Oct 2015 to 1,770 followers 4 0

RT @3DPrintGirl: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https:/…

Jorma Tevajärvi @JormaTevajrvi2:59 AM – 24 Oct 2015 to 1,770 followers 4 0

RT @3DPrintGirl: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/ifG…

Jorma Tevajärvi @JormaTevajrvi2:59 AM – 24 Oct 2015 to 1,770 followers 6 0

RT @3DPrintGirl: The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/pHo7wYeUQY

Jorma Tevajärvi @JormaTevajrvi2:59 AM – 24 Oct 2015 to 1,770 followers 15 0

RT @3DPrintGirl: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://…

Jorma Tevajärvi @JormaTevajrvi2:59 AM – 24 Oct 2015 to 1,770 followers 10 0

RT @3DPrintGirl: Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/WPhiwz…

Hosting Hub @HostinghubInfo2:49 AM – 24 Oct 2015 to 742 followers 10 0

RT @3DPrintGirl: Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/WPhiwz…

TheUPSStore2109 @TheUPSStore21092:14 AM – 24 Oct 2015 to 268 followers 2 0
RT @TheUPSStore: Tomorrow at #I3DPConf our expert panel is ready to answer your questions! Submit yours using #3DPWeek https://t.co/PsWXws8…

connie874cs@gmail.co @connie874cs1:56 AM – 24 Oct 2015 to 146 followers 10 0

RT @3DPrintGirl: Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/WPhiwz…

connie874cs@gmail.co @connie874cs1:50 AM – 24 Oct 2015 to 146 followers 1 0

RT @My3DPrinting: Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

3D Printing @Just_Print_It11:53 PM – 23 Oct 2015 to 839 followers 5 0

RT @GARTEL: Great day @ #I3DPConf, #CreativeForce #Design Competition in CA this week #3dprinting #gartel3d #makeprintable https://t.co/kq…

Jesus De La Maza @jmmaza211:49 PM – 23 Oct 2015 to 43 followers 1 0

RT @3Dkumo: Spectacular otherworldly violin made via 3D printer es.gizmodo.com/parece-el-arma… #3dprinting #3dprint #I3DPConf #additivemanufacturi…

Mixed Dimensions Inc @MXD3D10:21 PM – 23 Oct 2015 to 679 followers 1 0
RT @SJGrunewald: Had a demo with #makeprintable today. Amazing file repair service. Will auto generate walls & fix holes. #I3DPConf https:/…

Michael Makdah @MichaelMakdah10:21 PM – 23 Oct 2015 to 1,234 followers 5 0

RT @GARTEL: Great day @ #I3DPConf, #CreativeForce #Design Competition in CA this week #3dprinting #gartel3d #makeprintable https://t.co/kq…

Mixed Dimensions Inc @MXD3D10:21 PM – 23 Oct 2015 to 679 followers 5 0
RT @GARTEL: Great day @ #I3DPConf, #CreativeForce #Design Competition in CA this week #3dprinting #gartel3d #makeprintable https://t.co/kq…

Bunny Ellerin @BunnyEllerin9:42 PM – 23 Oct 2015 to 2,450 followers 0 0

So happy to hear that @metamason @LOKarpas & @cliffsarkin crushed it winning #I3DPConf start up $$. #SleepApnea https://t.co/TAf29vfS7C

Time4Hemp @Time4Hemp9:42 PM – 23 Oct 2015 to 41,815 followers 10 0

RT @3DPrintGirl: Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/WPhiwz…

ДедоМраз™ @Dedo_Mraz9:26 PM – 23 Oct 2015 to 849 followers 10 0
RT @3DPrintGirl: Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/WPhiwz…

Pinshape @pinshape9:25 PM – 23 Oct 2015 to 8,879 followers 10 0

RT @3DPrintGirl: Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/WPhiwz…

America Makes @AmericaMakes9:00 PM – 23 Oct 2015 to 4,938 followers 0 0

#AMmember @3dsystemscorp gave a captivating keynote at #I3DPConf; how #3DP can improve the quality of human life: bit.ly/1LSciDm

3D Printing @Just_Print_It7:03 PM – 23 Oct 2015 to 839 followers 6 0

RT @IntraconGlobal: #nTopology believes with a little development, #3Dprinted shirts like this are the future of your wardrobe #I3DPConf ht…

Made Right Here @MadeRightHereUS6:31 PM – 23 Oct 2015 to 365 followers 3 0

RT @3DPrintBoard: Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/uCnyD…

njmike731 @njmike7316:24 PM – 23 Oct 2015 to 921 followers 15 0

RT @3DPrintGirl: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://…

Gary Dawson Designs @GDawsonDesigns6:17 PM – 23 Oct 2015 to 111 followers 4 0

RT @3DForged: At the UPS Store Booth @ #I3DPConf. When UPS jumps into a new technology, you know it’s a big deal. #3dprinting https://t.co/…

Scansite @Scansite3D5:55 PM – 23 Oct 2015 to 13 followers 0 0
We had a great time at #I3DPConf. We’ll be in Phoenix next week for intl #sculpture conference #scanning #tech https://t.co/EYfmlMLG5D

Наталия Балакер @areadna45:06 PM – 23 Oct 2015 to 65 followers 15 0
RT @3DPrintGirl: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://…

3DPrint @3DPrint_com5:02 PM – 23 Oct 2015 to 33,757 followers 0 0

Thanks for a fantastic conference, #I3DPConf! We enjoyed meeting & seeing so many impressive innovators. Now back home we fly.
Intracon @IntraconGlobal4:39 PM – 23 Oct 2015 to 116 followers 0 1

Who wouldn’t love to jam on #3Dprinted instruments like this bass from @MONAD_Studio? #I3DPConf https://t.co/SrtwvP3cJm

Alex Carmona @carmona_alex4:16 PM – 23 Oct 2015 to 125 followers 2 0

RT @3DPrintingFans: Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

3DKUMO @3Dkumo4:12 PM – 23 Oct 2015 to 401 followers 1 1

Spectacular otherworldly violin made via 3D printer es.gizmodo.com/parece-el-arma… #3dprinting #3dprint #I3DPConf #additivemanufacturing #music

Anne Tramut @Annan263:47 PM – 23 Oct 2015 to 1,142 followers 10 0

RT @3DPrintGirl: Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/WPhiwz…

Makernauts @makernauts3:17 PM – 23 Oct 2015 to 310 followers 1 0

RT @3D_Print_News: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https:…

Retweet Detroit @retweetDetroit3:06 PM – 23 Oct 2015 to 1,280 followers 1 0

RT @3DPrint_com: Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/3VA04H…

Hayden Payne @HmajorP3:06 PM – 23 Oct 2015 to 186 followers 1 0

RT @My3DPrinting: Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/ALaHo…

3DPrintPress @3DPrintPress3:04 PM – 23 Oct 2015 to 194 followers 3 0

RT @3DPrintBoard: Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/uCnyD…

alex@fabbing @fabbing3:01 PM – 23 Oct 2015 to 8,565 followers 3 0

RT @3DPrintBoard: Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/uCnyD…

The UPS Store @TheUPSStore3:00 PM – 23 Oct 2015 to 11,688 followers 1 0
RT @Inside3DPrint: .@TheUPSStore: Personal Service 4 Small Businesses mcklr.me/1PJGguU (via @3DPrint_com) #I3DPConf #3dprinting http…

3D Printer News @3DPrinterNews3:00 PM – 23 Oct 2015 to 1,078 followers 0 0

twitter.com/3DPrintBoardco… Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! … https://t.co/Q7vyr2aj6M

Inside3DPrint @Inside3DPrint3:00 PM – 23 Oct 2015 to 3,143 followers 1 0

.@TheUPSStore: Personal Service 4 Small Businesses mcklr.me/1PJGguU (via @3DPrint_com) #I3DPConf #3dprinting https://t.co/mYApwmQi2Q

3D Printing Fans @3DPrintingFans2:59 PM – 23 Oct 2015 to 17,194 followers 0 0

Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/U160rHOqhz

3D Printing News @My3DPrinting2:59 PM – 23 Oct 2015 to 54,848 followers 1 0

Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/ALaHo1TD9J

3D Print .com @3DPrintBoard2:59 PM – 23 Oct 2015 to 46,499 followers 3 1

Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/uCnyDh54zx

3D Printing @3DPrintBoardcom2:59 PM – 23 Oct 2015 to 11,478 followers 0 0

Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/MGW0YZp5ks

cocowheatbaby @fungifunguy2:50 PM – 23 Oct 2015 to 188 followers 10 0

RT @3DPrintGirl: Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/WPhiwz…

Gartel Art Cars @GartelArtCars2:49 PM – 23 Oct 2015 to 851 followers 1 0

RT @SJGrunewald: Just met digital artist Laurence Gartel and held his 1st sculpture using a #3doodler could be worth $$$$! #I3DPConf https:…

カモノハシ @roso19542:40 PM – 23 Oct 2015 to 9,915 followers 0 0
＠3DPrintGirl: Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! … https://t.co/NEivPEPHFz

Michael Zeigle @MikeZeigle2:40 PM – 23 Oct 2015 to 1,204 followers 1 0

RT @3DPrint_com: Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup…

3D Printing @3D_Print_News2:38 PM – 23 Oct 2015 to 12,523 followers 0 0

Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/wpywNn8M16

3DPrint @3DPrint_com2:38 PM – 23 Oct 2015 to 33,757 followers 1 1

Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/3VA04Hn2T8

3D Printing News @3DNewsNow2:38 PM – 23 Oct 2015 to 3,314 followers 0 0
Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/5hOd4eVSTw

3D Printing @3D_Printing_it2:38 PM – 23 Oct 2015 to 2,209 followers 0 0

Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/jgogCQYHyn

3D Printing News @3DPrintGirl2:38 PM – 23 Oct 2015 to 40,617 followers 10 12

Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/WPhiwzVZjN

3DPrint @3DPrint_com2:38 PM – 23 Oct 2015 to 33,757 followers 1 0

Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup…

3D Printing News @3DNewsNow2:38 PM – 23 Oct 2015 to 3,314 followers 0 0
Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup…

3D Printing @3D_Printing_it2:38 PM – 23 Oct 2015 to 2,209 followers 0 0

Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup…

3D Printing @3D_Print_News2:38 PM – 23 Oct 2015 to 12,523 followers 0 0

Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup…

Fernando Rivas Navaz @ferrivasnav2:27 PM – 23 Oct 2015 to 110 followers 7 0

RT @3DPrintingIt: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https:/…

C.R.S. @ribeiroharman2:06 PM – 23 Oct 2015 to 1,562 followers 1 0

RT @3DPrint_com: Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

Mario Barra @mbvates2:02 PM – 23 Oct 2015 to 524 followers 3 0

RT @My3DPrinting: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https:/…

Yerald max @painterXman2:00 PM – 23 Oct 2015 to 61 followers 2 0

RT @3DPrintGirl: Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

Yerald max @painterXman2:00 PM – 23 Oct 2015 to 61 followers 1 0

RT @3D_Print_News: Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

Bazooka Tech @bazooka_tech1:59 PM – 23 Oct 2015 to 1,173 followers 2 0
RT @3DPrintingIt: Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

caterpila.blue @CaterpilaBlue1:58 PM – 23 Oct 2015 to 25 followers 3 0

RT @3DPrintBoard: Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

Rigging Artist @RiggingArtist1:52 PM – 23 Oct 2015 to 486 followers 3 0

RT @3DPrintBoard: Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

Rick Binkley @Binksterb1:48 PM – 23 Oct 2015 to 11,262 followers 2 0
RT @3DPrintingFans: Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

Julian Flores @blueocean54541:46 PM – 23 Oct 2015 to 2,385 followers 2 0
RT @3DPrintingIt: Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

alex@fabbing @fabbing1:46 PM – 23 Oct 2015 to 8,565 followers 3 0

RT @3DPrintBoard: Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

3D Print .com @3DPrintBoard1:45 PM – 23 Oct 2015 to 46,499 followers 3 1

Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

3D Printing Fans @3DPrintingFans1:45 PM – 23 Oct 2015 to 17,194 followers 2 0

Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

3D Printing @3DPrintingIt1:45 PM – 23 Oct 2015 to 15,317 followers 2 1

Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

3D Printing News @My3DPrinting1:45 PM – 23 Oct 2015 to 54,848 followers 1 2

Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

ASUGIT @ASUGIT1:34 PM – 23 Oct 2015 to 202 followers 6 0

RT @3DPrintGirl: The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/pHo7wYeUQY

Tesseract257 @Tesseract2571:32 PM – 23 Oct 2015 to 1,904 followers 2 0
RT @3DPrintGirl: Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

カモノハシ @roso19541:27 PM – 23 Oct 2015 to 9,915 followers 0 0
＠3DPrintGirl: Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

3D Printing News @3DNewsNow1:26 PM – 23 Oct 2015 to 3,314 followers 0 0
Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

3D Printing @3D_Print_News1:26 PM – 23 Oct 2015 to 12,523 followers 1 1

Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

3D Printing @3D_Printing_it1:26 PM – 23 Oct 2015 to 2,209 followers 0 0

Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

3D Printing News @3DPrintGirl1:26 PM – 23 Oct 2015 to 40,617 followers 2 1

Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

3DPrint @3DPrint_com1:26 PM – 23 Oct 2015 to 33,757 followers 1 1

Day two at #I3DPConf in Santa Clara had even more to see! 3dprint.com/102085/i3dpcon…

John Meckler @johnmeckler1:15 PM – 23 Oct 2015 to 325 followers 5 0

RT @ttranpham: Thanks to @Inside3DPrint for hosting a great metal #3Dprinting panel today | tuan.tranpham@arcam.com | #I3DPConf | https://t…

Formsfield @Formsfield1:00 PM – 23 Oct 2015 to 70 followers 0 0

#3dprinting can be useful everywhere even in space! #NASA #3dprinted #I3DPConf Read more here ow.ly/TKU5u

David VENANCIO @david_venancio11:36 AM – 23 Oct 2015 to 179 followers 2 0

RT @3DNewsNow: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://t.…

mikebit @77bergkamp1911:33 AM – 23 Oct 2015 to 580 followers 3 0

RT @MarkTrageser: #I3dpconf #insaniTOY. Santa Clara expo https://t.co/uS3MDvMgTF

mikebit @77bergkamp1911:33 AM – 23 Oct 2015 to 580 followers 6 0

RT @IntraconGlobal: #nTopology believes with a little development, #3Dprinted shirts like this are the future of your wardrobe #I3DPConf ht…

mikebit @77bergkamp1911:33 AM – 23 Oct 2015 to 580 followers 2 0

RT @GatewayCDI: .@3DForged: At UPS Store Booth @ #I3DPConf. When UPS jumps into new technology you know it’s a big deal. #3dprinting https:…

mikebit @77bergkamp1911:32 AM – 23 Oct 2015 to 580 followers 3 0

RT @GoEngineer: What would you scan if you had this #HandySCAN in hand? #3dprint #I3DPConf https://t.co/94VQoWiO5K

mikebit @77bergkamp1911:32 AM – 23 Oct 2015 to 580 followers 3 0

RT @kojaahasti: I asked these guys what’s new & they showed off their bio-based filaments like this one made with beer! #I3DPConf https://t…

The Candy Factory @TheCandyFactory10:42 AM – 23 Oct 2015 to 2,947 followers 2 0

RT @GreenfishLabs: Greenfish at #3dprinting #I3DPConf in Santa Clara today. https://t.co/VQlIzq7HjA
ZMorph 3D Printer @ZMorph3d8:55 AM – 23 Oct 2015 to 696 followers 1 0

RT @Inside3DPrint: .@ZMorph3d appeals to the #I3DPConf #Startup Competition judges https://t.co/QGb8A70RmP
Meme Veels @MemeVeels8:47 AM – 23 Oct 2015 to 124 followers 15 0

RT @3DPrintGirl: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://…

Wednesday Atoms @genericpanic8:30 AM – 23 Oct 2015 to 784 followers 2 0

RT @3DPrintingFans: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https…

Now Take Control @NowTakeControl8:10 AM – 23 Oct 2015 to 1,259 followers 15 0

RT @3DPrintGirl: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://…

Waititu James @Waititujames7:52 AM – 23 Oct 2015 to 1,066 followers 15 0

RT @3DPrintGirl: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://…

BayAreaStartupEvents @BayAreaStartup7:46 AM – 23 Oct 2015 to 609 followers 1 0

RT @madeforme3d: Great first day at the Inside 3D Printing Conference in Santa Clara #I3DPConf

Serpil Özgüven @serpil_ozguven7:33 AM – 23 Oct 2015 to 300 followers 15 0

RT @3DPrintGirl: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://…

Johnny Christensen @JohnnyChristen46:44 AM – 23 Oct 2015 to 165 followers 5 0

RT @ttranpham: Thanks to @Inside3DPrint for hosting a great metal #3Dprinting panel today | tuan.tranpham@arcam.com | #I3DPConf | https://t…

Made for Me @madeforme3d6:36 AM – 23 Oct 2015 to 208 followers 0 0

Great First Day @ #I3DPConf m.facebook.com/story.php?stor…

Made for Me @madeforme3d6:33 AM – 23 Oct 2015 to 208 followers 1 0

Great first day at the Inside 3D Printing Conference in Santa Clara #I3DPConf

Jade Le Maître @Aratta6:02 AM – 23 Oct 2015 to 3,470 followers 3 0
RT @TheUPSStore: So many industries are being touched by #3Dprinting! Today we’ve heard about great applications from dental to fashion! #3…

Jonathan Grothaus @jongrothaus5:45 AM – 23 Oct 2015 to 186 followers 3 0

RT @My3DPrinting: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https:/…

Serdar Okumuş @baykush5:43 AM – 23 Oct 2015 to 251 followers 7 0
RT @3DPrintingIt: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https:/…

futuristik|phuq|budi @tis0netrippn5:22 AM – 23 Oct 2015 to 303 followers 15 0

RT @3DPrintGirl: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://…

Yerald max @painterXman5:13 AM – 23 Oct 2015 to 61 followers 7 0

RT @3DPrintingIt: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https:/…

michele merli @1mago55:13 AM – 23 Oct 2015 to 233 followers 7 0
RT @brianfederal: Natacha Alpert speaks today @ Inside 3D Printing Santa Clara on #3DPrinting in #Fashion 1:30 #I3DPConf #3DPrinter https:/…

michele merli @1mago55:03 AM – 23 Oct 2015 to 233 followers 15 0
RT @3DPrintGirl: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://…

Fahad A AlGhammas @engfahadg4:31 AM – 23 Oct 2015 to 105 followers 8 0

RT @3DPrintBoard: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https:/…

ryan @ryang5733:23 AM – 23 Oct 2015 to 143 followers 7 0

RT @3DPrintingIt: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https:/…

Savvy Society @SavvySoc3:23 AM – 23 Oct 2015 to 99 followers 1 0

RT @3DPrint_com: Savvy Society is now presenting, finishing out the great lineup here #I3DPConf #StartupCompetition https://t.co/yNuiqWfo5w

Sathyan Nagarajan @SathyanBN3:22 AM – 23 Oct 2015 to 58 followers 15 0

RT @3DPrintGirl: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://…

Made In Space @MadeInSpace3:14 AM – 23 Oct 2015 to 4,801 followers 2 0
RT @3DPrint_com: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/nxW…

Pablo Boza @PabloBoza2:42 AM – 23 Oct 2015 to 1,009 followers 15 0

RT @3DPrintGirl: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://…

Inside3DPrint @Inside3DPrint2:33 AM – 23 Oct 2015 to 3,143 followers 5 0

RT @ttranpham: Thanks to @Inside3DPrint for hosting a great metal #3Dprinting panel today | tuan.tranpham@arcam.com | #I3DPConf | https://t…

James T Smith @jtsmith01012:16 AM – 23 Oct 2015 to 58 followers 1 0

RT @madeforme3d: Heading to the Inside 3D Printing Santa Clara keynote speech #I3DPConf https://t.co/dmYfIlr494

James T Smith @jtsmith01012:16 AM – 23 Oct 2015 to 58 followers 1 0

RT @madeforme3d: View from the exhibition floor at Inside 3D Printing Santa Clara #I3DPConf https://t.co/n8cvjB1XK1

Rapid Prototyping @rapidprototypin2:06 AM – 23 Oct 2015 to 3,396 followers 5 0

RT @ttranpham: Thanks to @Inside3DPrint for hosting a great metal #3Dprinting panel today | tuan.tranpham@arcam.com | #I3DPConf | https://t…

Rigging Artist @RiggingArtist1:58 AM – 23 Oct 2015 to 486 followers 5 0

RT @ttranpham: Thanks to @Inside3DPrint for hosting a great metal #3Dprinting panel today | tuan.tranpham@arcam.com | #I3DPConf | https://t…

Tuan TRANPHAM @ttranpham1:58 AM – 23 Oct 2015 to 4,047 followers 5 7

Thanks to @Inside3DPrint for hosting a great metal #3Dprinting panel today | tuan.tranpham@arcam.com | #I3DPConf | https://t.co/NXlT7VKOuf

mlnotaur1c @b777bbjvp1:54 AM – 23 Oct 2015 to 143 followers 2 0
RT @TheUPSStore: Successful day at #i3DPConf learning about the endless possibilities of #3Dprinting for all industries! #3DPWeek https://t…

Ludo.Inc @LudoStreamLine1:42 AM – 23 Oct 2015 to 25 followers 3 0

RT @3DPrintBoard: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t…
Ludo.Inc @LudoStreamLine1:41 AM – 23 Oct 2015 to 25 followers 2 0

RT @3DNewsNow: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://t.…

Inside3DPrint @Inside3DPrint1:39 AM – 23 Oct 2015 to 3,143 followers 2 0

RT @TheUPSStore: Successful day at #i3DPConf learning about the endless possibilities of #3Dprinting for all industries! #3DPWeek https://t…

Lori Cummings @lkcummings181:37 AM – 23 Oct 2015 to 129 followers 6 0

RT @3DPrintGirl: The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/pHo7wYeUQY

Allandnnn @allandnnn1:07 AM – 23 Oct 2015 to 783 followers 1 0

RT @Inside3DPrint: THANK YOU to our #I3DPConf program chairs! @Columbia Hod Lipson, @tbenst, @BunnyEllerin, @SandraKayHelsel, & @StewQ http…

Inside3DPrint @Inside3DPrint1:00 AM – 23 Oct 2015 to 3,143 followers 1 1

THANK YOU to our #I3DPConf program chairs! @Columbia Hod Lipson, @tbenst, @BunnyEllerin, @SandraKayHelsel, & @StewQ mcklr.me/1ZGBhzD

Inside3DPrint @Inside3DPrint12:52 AM – 23 Oct 2015 to 3,143 followers 0 1

What a fun event! Here’s one of @Metamason3D holding up their big check. #I3DPConf #3dprinting https://t.co/NIN2cL3as3

mare parker-otoole @zazencoyot12:33 AM – 23 Oct 2015 to 495 followers 7 0

RT @3DPrintGirl: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t.…
Hasti Afsarifard @kojaahasti12:28 AM – 23 Oct 2015 to 99 followers 2 0

RT @3DPrint_com: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/nxW…

Grant W. Tilden @AlienHunterG12:03 AM – 23 Oct 2015 to 43 followers 3 0

RT @GoEngineer: What would you scan if you had this #HandySCAN in hand? #3dprint #I3DPConf https://t.co/94VQoWiO5K

Helix Games @H3LIX_GAMES11:58 PM – 22 Oct 2015 to 95 followers 4 0
RT @3DPrintingIt: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https:…

Todd Atchison @flameskimmer11:56 PM – 22 Oct 2015 to 21 followers 3 0

RT @kojaahasti: I asked these guys what’s new & they showed off their bio-based filaments like this one made with beer! #I3DPConf https://t…

Margaret @hatzbymm11:47 PM – 22 Oct 2015 to 1,095 followers 7 0

RT @3DPrintingIt: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https:/…

Engineer3d @OzEngineer3d11:36 PM – 22 Oct 2015 to 1,405 followers 4 0

RT @brianfederal: The Future of Construction will be 3D Printed. Tony Capasso Inside 3D Printing #3DPrinting #I3DPConf https://t.co/f77wzUp…

Inside3DPrint @Inside3DPrint11:24 PM – 22 Oct 2015 to 3,143 followers 3 0

RT @TheUPSStore: So many industries are being touched by #3Dprinting! Today we’ve heard about great applications from dental to fashion! #3…

The UPS Store @TheUPSStore11:24 PM – 22 Oct 2015 to 11,688 followers 2 7
Successful day at #i3DPConf learning about the endless possibilities of #3Dprinting for all industries! #3DPWeek https://t.co/07EE5oha01

Inside3DPrint @Inside3DPrint11:21 PM – 22 Oct 2015 to 3,143 followers 3 0

RT @kojaahasti: I asked these guys what’s new & they showed off their bio-based filaments like this one made with beer! #I3DPConf https://t…

Inside3DPrint @Inside3DPrint11:20 PM – 22 Oct 2015 to 3,143 followers 3 0

RT @GoEngineer: What would you scan if you had this #HandySCAN in hand? #3dprint #I3DPConf https://t.co/94VQoWiO5K

printingspecialists1 @specialprinting11:20 PM – 22 Oct 2015 to 124 followers 15 0

RT @3DPrintGirl: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://…

Inside3DPrint @Inside3DPrint11:20 PM – 22 Oct 2015 to 3,143 followers 4 0

RT @brianfederal: The Future of Construction will be 3D Printed. Tony Capasso Inside 3D Printing #3DPrinting #I3DPConf https://t.co/f77wzUp…

alex@fabbing @fabbing11:19 PM – 22 Oct 2015 to 8,565 followers 4 0

RT @brianfederal: The Future of Construction will be 3D Printed. Tony Capasso Inside 3D Printing #3DPrinting #I3DPConf https://t.co/f77wzUp…

Dave Griessmann @dgriessmann11:14 PM – 22 Oct 2015 to 212 followers 8 0

RT @3DPrintBoard: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https:/…

Inside3DPrint @Inside3DPrint11:00 PM – 22 Oct 2015 to 3,143 followers 0 1

End your #I3DPConf Santa Clara experience w/ @tbenst & explore the future of #3dprinting mcklr.me/1QxU1uY https://t.co/8WXtrxW3Mt

Brian Federal @brianfederal10:49 PM – 22 Oct 2015 to 10,290 followers 4 2

The Future of Construction will be 3D Printed. Tony Capasso Inside 3D Printing #3DPrinting #I3DPConf https://t.co/f77wzUpvYX

Sovanna Mam @SovannaMam10:49 PM – 22 Oct 2015 to 385 followers 2 0

RT @GreenfishLabs: Greenfish at #3dprinting #I3DPConf in Santa Clara today. https://t.co/VQlIzq7HjA
SF Fashtech @sffashiontech10:41 PM – 22 Oct 2015 to 2,679 followers 2 0

RT @3DPrint_com: Congratulations to the winners! 1st: Metamason 2nd: Savvy Society 3rd: AstroPrint #I3DPConf #StartupCompetition

AccountingSuite @AccountingSuite10:35 PM – 22 Oct 2015 to 594 followers 3 0

RT @Inside3DPrint: “The world has seen enough great products; it’s time we start designing perfect products,” says @Metamason3D #I3DPConf #…

Inside3DPrint @Inside3DPrint10:35 PM – 22 Oct 2015 to 3,143 followers 0 0

.@RiverVR GM @SivanIram Gave Keynote to #VRSummit & #3DPrinting Attendees at #I3DPConf mcklr.me/1MH5zZh #virtualreality @rothventures

The UPS Store @TheUPSStore10:25 PM – 22 Oct 2015 to 11,688 followers 0 1
#Maker, @MarkTrageser, shows 3D printing’s playful side with #Insanitoy at #I3DPConf #3DPWeek https://t.co/H5xcbzMLIz

Yoni Binstock @YoniBinstock10:15 PM – 22 Oct 2015 to 462 followers 0 1

At #i3dpconf? Then you should check out the top eBook on #3DPrinting #Entrepreneurship – bit.ly/3DEntrepreneur

3D Printing newsbot @3DPrint_newsbot10:11 PM – 22 Oct 2015 to 7,756 followers 2 0

RT @TheUPSStore: Another awesome industry using #3Dprint technology – #music! Check out @MONAD_Studio’s jam session at #I3DPConf! https://t…

GoEngineer @GoEngineer10:02 PM – 22 Oct 2015 to 1,689 followers 3 3

What would you scan if you had this #HandySCAN in hand? #3dprint #I3DPConf https://t.co/94VQoWiO5K
3D Printing @Just_Print_It10:00 PM – 22 Oct 2015 to 839 followers 7 0

RT @brianfederal: Natacha Alpert speaks today @ Inside 3D Printing Santa Clara on #3DPrinting in #Fashion 1:30 #I3DPConf #3DPrinter https:/…

AccountingSuite @AccountingSuite9:55 PM – 22 Oct 2015 to 594 followers 0 1

3D Printing: Personalized Production for the Mass Market by @VLab in 2012! #I3DPconf @inside3dprint youtu.be/sD4JAzp5E3c

kurt kunselman @mbaforyou9:55 PM – 22 Oct 2015 to 104 followers 0 1
3D Printing: Personalized Production for the Mass Market by @VLab in 2012! #I3DPconf @inside3dprint youtu.be/sD4JAzp5E3c

Hasti Afsarifard @kojaahasti9:52 PM – 22 Oct 2015 to 99 followers 3 3

I asked these guys what’s new & they showed off their bio-based filaments like this one made with beer! #I3DPConf https://t.co/mmSLeuyJUL

Intracon @IntraconGlobal9:47 PM – 22 Oct 2015 to 116 followers 4 0

RT @Inside3DPrint: “We can’t afford to have half of our brightest minds opting out,” says Alexa Fleischman, @SavvySoc #3dprinting #startupp…

Teacher with #VR @ClementAdert9:43 PM – 22 Oct 2015 to 275 followers 0 1

RT ‘Will the Future of #VR Be #Mobile?’ Panel Debated @ #VRSummit, #I3DPConf … https://t.co/pmi7OED70C https://t.co/gCBe2KgjUd
Kegan Schouwenburg @Kegan3D9:42 PM – 22 Oct 2015 to 2,101 followers 3 0
RT @Inside3DPrint: “The world has seen enough great products; it’s time we start designing perfect products,” says @Metamason3D #I3DPConf #…

Savvy Society @SavvySoc9:40 PM – 22 Oct 2015 to 99 followers 2 0
RT @Inside3DPrint: And congratulations to our 2nd and 3rd place winners @savvysoc and @AstroPrint3D! #I3DPConf

la moustache ringard @SJGrunewald9:39 PM – 22 Oct 2015 to 234 followers 0 1

@autodesk will incorporate #netfabb software into #spark #I3DPConf

Dale Sinclair @dale_sinclair9:39 PM – 22 Oct 2015 to 406 followers 2 0

RT @3DPrintingFans: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https…

John Meckler @johnmeckler9:37 PM – 22 Oct 2015 to 325 followers 2 0

RT @Inside3DPrint: Congratulations @Metamason3D for winning 1st place in the #startup competition! #I3DPConf https://t.co/plnQtSATao

Inside3DPrint @Inside3DPrint9:35 PM – 22 Oct 2015 to 3,143 followers 0 0

‘Will the Future of #VR Be #Mobile?’ Panel Debated @ #VRSummit, #I3DPConf mcklr.me/1GXnQzL #virtualreality https://t.co/aAUqU7u7Rl

la moustache ringard @SJGrunewald9:34 PM – 22 Oct 2015 to 234 followers 0 1

Takeaway from keynote so far. Autodesk wants #Spark to be #android for 3dprinting. #I3DPConf

Music Host Network @MusicHostNet9:34 PM – 22 Oct 2015 to 11,540 followers 2 0

RT @TheUPSStore: Another awesome industry using #3Dprint technology – #music! Check out @MONAD_Studio’s jam session at #I3DPConf! https://t…

Larry B. Williams @vplarryb9:34 PM – 22 Oct 2015 to 759 followers 15 0

RT @3DPrintGirl: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://…

Inside3DPrint @Inside3DPrint9:32 PM – 22 Oct 2015 to 3,143 followers 0 1

View from the back of a crowded #I3DPConf Keynote session ft @benschrauwen on @Spark3DP, @autodesk https://t.co/BjpmmBqn31

Bazooka Tech @bazooka_tech9:32 PM – 22 Oct 2015 to 1,173 followers 2 0
RT @typeamachines: #3DPrinting in Spaaace! (spaaaace! spaaace!) #I3DPConf hubs.ly/H01j_c10 https://t.co/ZFAHhdtMJw

AstroPrint @AstroPrint3D9:26 PM – 22 Oct 2015 to 856 followers 2 0

RT @Inside3DPrint: And congratulations to our 2nd and 3rd place winners @savvysoc and @AstroPrint3D! #I3DPConf

Inside3DPrint @Inside3DPrint9:26 PM – 22 Oct 2015 to 3,143 followers 2 5

And congratulations to our 2nd and 3rd place winners @savvysoc and @AstroPrint3D! #I3DPConf

The UPS Store @TheUPSStore9:25 PM – 22 Oct 2015 to 11,688 followers 2 0
RT @Inside3DPrint: Congratulations @Metamason3D for winning 1st place in the #startup competition! #I3DPConf https://t.co/plnQtSATao

christine trageser @christinatee9:24 PM – 22 Oct 2015 to 203 followers 0 2

Thanks @MecklerMedia @Avatarium3D & #I3DPConf!! I just got myself scanned for action figure immortality!
AstroPrint @AstroPrint3D9:23 PM – 22 Oct 2015 to 856 followers 2 0

RT @3DPrint_com: Congratulations to the winners! 1st: Metamason 2nd: Savvy Society 3rd: AstroPrint #I3DPConf #StartupCompetition

Lauren Wallace @SavvyLaur9:21 PM – 22 Oct 2015 to 99 followers 4 0

RT @Inside3DPrint: “We can’t afford to have half of our brightest minds opting out,” says Alexa Fleischman, @SavvySoc #3dprinting #startupp…

Savvy Society @SavvySoc9:21 PM – 22 Oct 2015 to 99 followers 4 0
RT @Inside3DPrint: “We can’t afford to have half of our brightest minds opting out,” says Alexa Fleischman, @SavvySoc #3dprinting #startupp…

la moustache ringard @SJGrunewald9:20 PM – 22 Oct 2015 to 234 followers 0 0

Time for closing keynote with #Autodesk Ben Schrauwen dir of #spark platform #I3DPConf

Inside3DPrint @Inside3DPrint9:19 PM – 22 Oct 2015 to 3,143 followers 2 6

Congratulations @Metamason3D for winning 1st place in the #startup competition! #I3DPConf https://t.co/plnQtSATao

Heather Archuletta @Pillownaut9:19 PM – 22 Oct 2015 to 8,241 followers 1 0

RT @kojaahasti: I always feel like a kid in a candy store at 3D printing conferences #I3DPConf pic.twitter.com/cArXG7PhNH

3DPrint @3DPrint_com9:19 PM – 22 Oct 2015 to 33,757 followers 2 4

Congratulations to the winners! 1st: Metamason 2nd: Savvy Society 3rd: AstroPrint #I3DPConf #StartupCompetition

Hasti Afsarifard @kojaahasti9:18 PM – 22 Oct 2015 to 99 followers 1 6

I always feel like a kid in a candy store at 3D printing conferences #I3DPConf pic.twitter.com/cArXG7PhNH

SF Fashtech @sffashiontech9:18 PM – 22 Oct 2015 to 2,679 followers 4 0

RT @Inside3DPrint: “We can’t afford to have half of our brightest minds opting out,” says Alexa Fleischman, @SavvySoc #3dprinting #startupp…

SF Fashtech @sffashiontech9:17 PM – 22 Oct 2015 to 2,679 followers 2 0

RT @typeamachines: #3DPrinting in Spaaace! (spaaaace! spaaace!) #I3DPConf hubs.ly/H01j_c10 https://t.co/ZFAHhdtMJw

3DPrint @3DPrint_com9:17 PM – 22 Oct 2015 to 33,757 followers 0 0

Congratulations to Metamason! Winner, #I3DPConf #StartupCompetition https://t.co/fnjaJZy1f1
SF Fashtech @sffashiontech9:17 PM – 22 Oct 2015 to 2,679 followers 1 0

RT @Inside3DPrint: “#Customization connects you to your own personal #symmetry” Natacha Alpert, @caleresinc #I3DPConf #3dscanning https://t…

la moustache ringard @SJGrunewald9:17 PM – 22 Oct 2015 to 234 followers 1 2

Just met digital artist Laurence Gartel and held his 1st sculpture using a #3doodler could be worth $$$$! #I3DPConf https://t.co/R4fJezroJe

Inside3DPrint @Inside3DPrint9:15 PM – 22 Oct 2015 to 3,143 followers 0 0

LAST #I3DPConf KEYNOTE ‘The Future of Making Things’ w/ @benschrauwen mcklr.me/1OFSiER @autodesk #3dprinting https://t.co/pQlk9u4IxL

Chris @NamesAreTrivial9:15 PM – 22 Oct 2015 to 318 followers 7 0

RT @3DPrintingIt: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https:/…

The UPS Store @TheUPSStore9:14 PM – 22 Oct 2015 to 11,688 followers 2 3
Another awesome industry using #3Dprint technology – #music! Check out @MONAD_Studio’s jam session at #I3DPConf! https://t.co/BneleCS4bG

3D Printing Info ™ @Info3DPrinting9:14 PM – 22 Oct 2015 to 115 followers 8 0

RT @3DPrintBoard: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https:/…

Inside3DPrint @Inside3DPrint9:12 PM – 22 Oct 2015 to 3,143 followers 0 0

Announcing the #startup competition winner in just a few! Head to the keynote room to find out. #I3DPConf

Eric de Marylebone @EricdeMarylebon9:10 PM – 22 Oct 2015 to 3,566 followers 15 0

RT @3DPrintGirl: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://…

Bazooka Tech @bazooka_tech9:08 PM – 22 Oct 2015 to 1,173 followers 7 0
RT @3DPrintingIt: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https:/…

alex@fabbing @fabbing9:08 PM – 22 Oct 2015 to 8,565 followers 8 0

RT @3DPrintBoard: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https:/…

Eric de Marylebone @EricdeMarylebon9:06 PM – 22 Oct 2015 to 3,566 followers 8 0

RT @3DPrintBoard: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https:/…

Patrick O’Neil @Padsuk9:06 PM – 22 Oct 2015 to 7,730 followers 1 0
RT @3DPrintBoardcom: ‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf http…

3D Print .com @3DPrintBoard9:05 PM – 22 Oct 2015 to 46,499 followers 8 4

‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://t.co/CoNsr1MtgW

3D Printing News @My3DPrinting9:05 PM – 22 Oct 2015 to 54,848 followers 3 2

‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://t.co/3txMgbyCII

3DPrint @3DPrint_com9:05 PM – 22 Oct 2015 to 33,757 followers 0 2

‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://t.co/dh0ZcQ4lac

3D Printing News @3DNewsNow9:05 PM – 22 Oct 2015 to 3,314 followers 2 1
‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://t.co/SbAEdWxilN

3D Printing @3D_Print_News9:05 PM – 22 Oct 2015 to 12,523 followers 1 1

‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://t.co/f5psLuvI5P

3D Printing @3D_Printing_it9:05 PM – 22 Oct 2015 to 2,209 followers 0 0

‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://t.co/o0Yn77MlrN

3D Printing @3DPrintingIt9:05 PM – 22 Oct 2015 to 15,317 followers 7 7

‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://t.co/uuK5b9qOsu

3D Printing Fans @3DPrintingFans9:05 PM – 22 Oct 2015 to 17,194 followers 2 1

‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://t.co/WLm4flV63K

3D Printing @3DPrintBoardcom9:05 PM – 22 Oct 2015 to 11,478 followers 1 1

‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://t.co/YvufXcD1vA

3D Printing News @3DPrintGirl9:05 PM – 22 Oct 2015 to 40,617 followers 15 7

‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://t.co/HVwyox6JAj

楽しい萬太郎 @happymantarou9:01 PM – 22 Oct 2015 to 690 followers 4 0
RT @3D_Print_News: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://…

3D Print Design Show @3dpdesignshow9:00 PM – 22 Oct 2015 to 574 followers 0 0
2:15PM Final Keynote: ‘The Future of Making Things’ ft @autodesk’s @benschrauwen mcklr.me/1OFSiER #3dprinting #I3DPConf

la moustache ringard @SJGrunewald8:55 PM – 22 Oct 2015 to 234 followers 0 1

Saw #lulzbotmini flexistruder in action. Still super quiet & the 0.6mm nozzle doesnt effect detail at all #I3DPConf https://t.co/qkliwfz2pL

Made for Me @madeforme3d8:52 PM – 22 Oct 2015 to 208 followers 1 2

View from the exhibition floor at Inside 3D Printing Santa Clara #I3DPConf https://t.co/n8cvjB1XK1
Tyler Pelletier @TylerInNiagara8:52 PM – 22 Oct 2015 to 290 followers 7 0

RT @3DPrintGirl: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t.…
Inside3DPrint @Inside3DPrint8:52 PM – 22 Oct 2015 to 3,143 followers 1 1

“#Customization connects you to your own personal #symmetry” Natacha Alpert, @caleresinc #I3DPConf #3dscanning https://t.co/Tsm8mJ36zX

Made for Me @madeforme3d8:51 PM – 22 Oct 2015 to 208 followers 1 3

Heading to the Inside 3D Printing Santa Clara keynote speech #I3DPConf https://t.co/dmYfIlr494
Stewart Quealy @StewQ8:46 PM – 22 Oct 2015 to 8,204 followers 0 2
Eric Goldemberg of @MONAD_Studio on 3D printed architecture & musical instruments. #I3DPConf https://t.co/SkkuW2FXPZ

FREDDY TINAJERO @Optrom_Ecuador8:37 PM – 22 Oct 2015 to 87 followers 4 0

RT @3D_Print_News: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://…

la moustache ringard @SJGrunewald8:35 PM – 22 Oct 2015 to 234 followers 1 2

Had a demo with #makeprintable today. Amazing file repair service. Will auto generate walls & fix holes. #I3DPConf https://t.co/CIriS0PT4p

Maureen @soaprookie8:34 PM – 22 Oct 2015 to 706 followers 3 0
RT @TheUPSStore: So many industries are being touched by #3Dprinting! Today we’ve heard about great applications from dental to fashion! #3…

Greenfish Labs @GreenfishLabs8:30 PM – 22 Oct 2015 to 690 followers 2 5

Greenfish at #3dprinting #I3DPConf in Santa Clara today. https://t.co/VQlIzq7HjA

Negi @nara02048:30 PM – 22 Oct 2015 to 249 followers 0 1
The world’s smallest #3dprinter ? Cute little piece of machine. #M3D #I3DPConf https://t.co/yoy8lhCN4L
The UPS Store @TheUPSStore8:30 PM – 22 Oct 2015 to 11,688 followers 3 4
So many industries are being touched by #3Dprinting! Today we’ve heard about great applications from dental to fashion! #3DPWeek #I3DPConf

covertress @covertress8:30 PM – 22 Oct 2015 to 9,294 followers 3 0

RT @Inside3DPrint: Check out the @MONAD_Studio booth for #3dprinted instruments #I3DPConf https://t.co/OIz4jNKxkV

TheKnotsOfLibra @TheKnotsOfLibra8:28 PM – 22 Oct 2015 to 77 followers 4 0

RT @3D_Print_News: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://…

Geo @GeoDelGonzo8:27 PM – 22 Oct 2015 to 153 followers 3 0

RT @Inside3DPrint: Check out the @MONAD_Studio booth for #3dprinted instruments #I3DPConf https://t.co/OIz4jNKxkV

Tommy Keiser @TommyKeiser8:22 PM – 22 Oct 2015 to 223 followers 3 0

RT @Inside3DPrint: Check out the @MONAD_Studio booth for #3dprinted instruments #I3DPConf https://t.co/OIz4jNKxkV

Type A Machines @typeamachines8:22 PM – 22 Oct 2015 to 1,256 followers 2 1

#3DPrinting in Spaaace! (spaaaace! spaaace!) #I3DPConf hubs.ly/H01j_c10 https://t.co/ZFAHhdtMJw
Inside3DPrint @Inside3DPrint8:15 PM – 22 Oct 2015 to 3,143 followers 3 2

Check out the @MONAD_Studio booth for #3dprinted instruments #I3DPConf https://t.co/OIz4jNKxkV
Uformia @Uformia8:02 PM – 22 Oct 2015 to 411 followers 1 0

RT @IntraconGlobal: With #thenaturegame, they are inviting creators to attempt to recreate organic complexity with #3Dprinting. #I3DPConf

3D Print Association @3DPrintAssoc7:53 PM – 22 Oct 2015 to 4,524 followers 3 0

RT @Inside3DPrint: “The world has seen enough great products; it’s time we start designing perfect products,” says @Metamason3D #I3DPConf #…

#30DaysStartup @Startup1247:52 PM – 22 Oct 2015 to 1,962 followers 0 1
#30DaysStartup j.mp/eventsaddle 3 minute pitches. 3 minute QA’s. 7 competitors. Who will win the #I3DPConf… https://t.co/QdEDTtBMVr

The UPS Store @TheUPSStore7:51 PM – 22 Oct 2015 to 11,688 followers 0 0
3 minute pitches. 3 minute QA’s. 7 competitors. Who will win the #I3DPConf #Startup Competition? #3DPWeek https://t.co/noCl9i8uoI

Tuan TRANPHAM @ttranpham7:49 PM – 22 Oct 2015 to 4,047 followers 2 0

RT @Inside3DPrint: .@winsheldon of SLM Solutions: impact of disruptive forces at the moment is limited #I3DPConf #3dprinting #metal https:/…

3DMetalPrintMag @3DMetalPrintMag7:49 PM – 22 Oct 2015 to 40 followers 1 0

RT @Inside3DPrint: #IonPath takes the stage w/ low-cost #metal #3dprinting #startuppitch #I3DPConf

3DMetalPrintMag @3DMetalPrintMag7:48 PM – 22 Oct 2015 to 40 followers 4 0

RT @TheUPSStore: “The industry has never been stronger!” @TerryWohlers at #I3DPConf #3DPWeek #3Dprinting

3DPrint @3DPrint_com7:47 PM – 22 Oct 2015 to 33,757 followers 0 0

Winner to be announced this afternoon! #I3DPConf #StartupCompetition

Inside3DPrint @Inside3DPrint7:42 PM – 22 Oct 2015 to 3,143 followers 4 4

“We can’t afford to have half of our brightest minds opting out,” says Alexa Fleischman, @SavvySoc #3dprinting #startuppitch #I3DPConf

Terry Wohlers @TerryWohlers7:42 PM – 22 Oct 2015 to 3,756 followers 2 0

RT @Inside3DPrint: “The industry has never been stronger.” – @TerryWohlers during @TheUPSStore #AskTheExperts panel #3DPWeek #I3DPConf #3dp…

Terry Wohlers @TerryWohlers7:41 PM – 22 Oct 2015 to 3,756 followers 2 0

RT @Inside3DPrint: “The next frontier in #3dprinting is #manufacturing” @TerryWohlers, @TheUPSStore #I3DPConf #AskTheExperts

3DPrint @3DPrint_com7:41 PM – 22 Oct 2015 to 33,757 followers 1 1

Savvy Society is now presenting, finishing out the great lineup here #I3DPConf #StartupCompetition https://t.co/yNuiqWfo5w

J. Nayer Hardin @cureworks7:41 PM – 22 Oct 2015 to 4,587 followers 4 0
RT @TheUPSStore: “The industry has never been stronger!” @TerryWohlers at #I3DPConf #3DPWeek #3Dprinting

3DPrinting on Campus @3DPonCampus7:40 PM – 22 Oct 2015 to 744 followers 3 0
RT @TheUPSStore: “#3Dprinting in classrooms has a great opportunity to get children excited about CAD design, engineering & sciences!” #3DP…

Stratasys @3d_printers7:39 PM – 22 Oct 2015 to 50,066 followers 4 0
RT @TheUPSStore: “The industry has never been stronger!” @TerryWohlers at #I3DPConf #3DPWeek #3Dprinting

Stratasys @Stratasys7:38 PM – 22 Oct 2015 to 32,354 followers 2 0
RT @TheUPSStore: The #i3DPConf Ask the Experts Panel w/ @TerryWohlers & Rich Garrity of #Stratasys @3d_printers has begun! #3DPWeek https:…

Type A Machines @typeamachines7:37 PM – 22 Oct 2015 to 1,256 followers 0 0

The Implications between #3DPrinting and Copyright Law. #I3DPConf hubs.ly/H01j_9K0 https://t.co/8Rrd57OnL8
James T Smith @jtsmith01017:36 PM – 22 Oct 2015 to 58 followers 1 0

RT @madeforme3d: Made For Me is expanding! We’re excited to be at #I3DPConf in Santa Clara meeting new customers and suppliers. https://t.…
Made for Me @madeforme3d7:35 PM – 22 Oct 2015 to 208 followers 1 1

Made For Me is expanding! We’re excited to be at #I3DPConf in Santa Clara meeting new customers and suppliers. https://t.co/CDM19FiffU
Derek Kaye @DerekKaye7:35 PM – 22 Oct 2015 to 5,132 followers 4 0
RT @Inside3DPrint: .@3DPrinterOS kicks off the #I3DPConf #Startup Competition! #3dprinting https://t.co/mVnZuRwwyb

Will Espada @EspadaGiclee7:34 PM – 22 Oct 2015 to 537 followers 4 0

RT @Inside3DPrint: .@3DPrinterOS kicks off the #I3DPConf #Startup Competition! #3dprinting https://t.co/mVnZuRwwyb

AstroPrint @AstroPrint3D7:34 PM – 22 Oct 2015 to 856 followers 1 0

RT @Inside3DPrint: “@AstroPrint3D is the #android of the #3dprinting world,” says CEO in #startup #pitch #I3DPConf

AstroPrint @AstroPrint3D7:34 PM – 22 Oct 2015 to 856 followers 1 0

RT @Inside3DPrint: Next up: @AstroPrint3D #I3DPConf #Startup Competition #3dprinting https://t.co/yFHOVQVcGb
3DPrint @3DPrint_com7:34 PM – 22 Oct 2015 to 33,757 followers 0 0

AstroPrint is up now at the #StartupCompetition #I3DPConf https://t.co/l6jeQEe0nS

Inside3DPrint @Inside3DPrint7:33 PM – 22 Oct 2015 to 3,143 followers 1 2

Next up: @AstroPrint3D #I3DPConf #Startup Competition #3dprinting https://t.co/yFHOVQVcGb
Inside3DPrint @Inside3DPrint7:33 PM – 22 Oct 2015 to 3,143 followers 1 2

“@AstroPrint3D is the #android of the #3dprinting world,” says CEO in #startup #pitch #I3DPConf

Julie Maes @julie_maes27:32 PM – 22 Oct 2015 to 2 followers 4 0

RT @Inside3DPrint: Todd Pietila & Jon Detterich: #3dprinted #hearts give sick babies 2nd chances @MaterialiseNV @ChildrensLA #I3DPConf http…

Inside3DPrint @Inside3DPrint7:29 PM – 22 Oct 2015 to 3,143 followers 3 2

“The world has seen enough great products; it’s time we start designing perfect products,” says @Metamason3D #I3DPConf #startuppitch

NYC Health Business @NYCHBL7:27 PM – 22 Oct 2015 to 1,655 followers 2 0
RT @BunnyEllerin: Amazing presentation by Jeff Huber @StandardCyborg on personalized manufacturing of prosthetics #I3DPConf https://t.co/z6…

Inside3DPrint @Inside3DPrint7:27 PM – 22 Oct 2015 to 3,143 followers 0 1

.@Metamason3D starts #startup competition pitch w/ patient specific #CPAP masks #3dprinting #3dscanning #I3DPConf

Inside3DPrint @Inside3DPrint7:27 PM – 22 Oct 2015 to 3,143 followers 0 0

.@Metamason3D presents their #I3DPConf #Startup Competition pitch https://t.co/jSBRO7l6di
3DPrint @3DPrint_com7:26 PM – 22 Oct 2015 to 33,757 followers 0 0

Speaking now: Metamason #StartupCompetition #I3DPConf https://t.co/JkzdagOpCX

Rapid Prototyping @rapidprototypin7:24 PM – 22 Oct 2015 to 3,396 followers 1 0

RT @Forecast3D: Up next on the #3DP #RaceCar: Hood Vents #3dprinted in FDM. Come see 4 urself @ #I3DPConf! #3dprinting #3DThursday https://…

la moustache ringard @SJGrunewald7:24 PM – 22 Oct 2015 to 234 followers 0 0

The #m3d is a surprisingly sophisticated 3D printer for only costing $450. And 100% made & assemble in us #I3DPConf https://t.co/MBulyhI5W5

Inside3DPrint @Inside3DPrint7:21 PM – 22 Oct 2015 to 3,143 followers 0 0

#Ionpath at the #I3DPConf #Startup Competition, up now https://t.co/Ph7SJ6SKPt

Inside3DPrint @Inside3DPrint7:20 PM – 22 Oct 2015 to 3,143 followers 1 0

#IonPath takes the stage w/ low-cost #metal #3dprinting #startuppitch #I3DPConf

3DPrint @3DPrint_com7:20 PM – 22 Oct 2015 to 33,757 followers 0 0

Now presenting: IonPath #I3DPConf #StartupCompetition https://t.co/MMphP10kCn

Balad Prosperity @IAMstudy20157:16 PM – 22 Oct 2015 to 176 followers 1 0

RT @3DPrint_com: Now: SJ Park from South Korea – based Owl Works #StartupCompetition #I3DPConf https://t.co/ddorCkslcs

Inside3DPrint @Inside3DPrint7:15 PM – 22 Oct 2015 to 3,143 followers 0 0

tweet @ us w/ “#I3DPConf”! What were your favorite sessions in Santa Clara so far? https://t.co/hXPjb8pJK6
Bridges to Italy @bridges2italy7:14 PM – 22 Oct 2015 to 6,734 followers 4 0

RT @Inside3DPrint: .@3DPrinterOS kicks off the #I3DPConf #Startup Competition! #3dprinting https://t.co/mVnZuRwwyb

Inside3DPrint @Inside3DPrint7:14 PM – 22 Oct 2015 to 3,143 followers 1 4

.@ZMorph3d appeals to the #I3DPConf #Startup Competition judges https://t.co/QGb8A70RmP
3DPrinterOS @3DPrinterOS7:13 PM – 22 Oct 2015 to 1,794 followers 4 0
RT @Inside3DPrint: .@3DPrinterOS kicks off the #I3DPConf #Startup Competition! #3dprinting https://t.co/mVnZuRwwyb

3DPrint @3DPrint_com7:13 PM – 22 Oct 2015 to 33,757 followers 0 1

Now: Zmorph! #I3DPConf #StartupCompetition https://t.co/JXwg3TXUpr

Inside3DPrint @Inside3DPrint7:12 PM – 22 Oct 2015 to 3,143 followers 0 0

.@ZMorph3d: personal fabrication for #creative people #startuppitch #I3DPConf

Fastener News Desk @FastenerNews7:11 PM – 22 Oct 2015 to 3,450 followers 3 0
RT @TheUPSStore: “#3Dprinting in classrooms has a great opportunity to get children excited about CAD design, engineering & sciences!” #3DP…

Forecast3D @Forecast3D7:09 PM – 22 Oct 2015 to 172 followers 1 0
Up next on the #3DP #RaceCar: Hood Vents #3dprinted in FDM. Come see 4 urself @ #I3DPConf! #3dprinting #3DThursday https://t.co/ptbBouKkoA

Inside3DPrint @Inside3DPrint7:09 PM – 22 Oct 2015 to 3,143 followers 0 1

#OwlWorks pitches at #I3DPConf #Startup Competition #3dprinting #morpheus https://t.co/hoPVNv4FVf
3DPrint @3DPrint_com7:08 PM – 22 Oct 2015 to 33,757 followers 1 0

Now: SJ Park from South Korea – based Owl Works #StartupCompetition #I3DPConf https://t.co/ddorCkslcs
Intracon @IntraconGlobal7:08 PM – 22 Oct 2015 to 116 followers 0 1

In the near future, these solutions could be custom-designed via mobile to meet the user’s specific needs. #I3DPConf https://t.co/zXQn3hHIY5

Winthrop Sheldon @winsheldon7:07 PM – 22 Oct 2015 to 161 followers 2 0

RT @Inside3DPrint: .@winsheldon of SLM Solutions: impact of disruptive forces at the moment is limited #I3DPConf #3dprinting #metal https:/…

Intracon @IntraconGlobal7:05 PM – 22 Oct 2015 to 116 followers 0 3

Some initial designs and prototypes of assistive #3dprinting technology. #I3DPConf https://t.co/TBNyOk4szb
Emily Gilbert @EmilyGi527696537:03 PM – 22 Oct 2015 to 1,373 followers 0 0

RT twitter.com/Inside3DPrint/… #3dprinting extends to #ear #cartilage w/ Dr. James Yoo, wakehealth, at #I3DPConf #med… https://t.co/ZCyap5Rvte

Inside3DPrint @Inside3DPrint7:02 PM – 22 Oct 2015 to 3,143 followers 3 0

RT @TheUPSStore: “#3Dprinting in classrooms has a great opportunity to get children excited about CAD design, engineering & sciences!” #3DP…

Intracon @IntraconGlobal7:00 PM – 22 Oct 2015 to 116 followers 0 2

Thoughts from the Scott Summit keynote: it’s not just about #3Dprinting, it’s about people helping people with the technology. #I3DPConf

The UPS Store @TheUPSStore7:00 PM – 22 Oct 2015 to 11,688 followers 3 1
“#3Dprinting in classrooms has a great opportunity to get children excited about CAD design, engineering & sciences!” #3DPWeek #i3DPConf

Inside3DPrint @Inside3DPrint6:59 PM – 22 Oct 2015 to 3,143 followers 4 3

.@3DPrinterOS kicks off the #I3DPConf #Startup Competition! #3dprinting https://t.co/mVnZuRwwyb
Inside3DPrint @Inside3DPrint6:59 PM – 22 Oct 2015 to 3,143 followers 0 0

The winner of the #startup competition will receive a $15K uncapped convertible note courtesy of #AsimovVentures! #I3DPConf

3DPrint @3DPrint_com6:58 PM – 22 Oct 2015 to 33,757 followers 0 1

First up: 3DPrinterOS #StartupCompetition #I3DPConf https://t.co/maV1sufEmf

la moustache ringard @SJGrunewald6:57 PM – 22 Oct 2015 to 234 followers 0 1

Great panel on how #3dprinting is completely changing toy business by #insanitoy founder Mark Trageser #I3DPConf https://t.co/d36YCWdiwY

Inside3DPrint @Inside3DPrint6:56 PM – 22 Oct 2015 to 3,143 followers 0 0

.@tbenst is getting the #startup competition started! #AsimovVentures #I3DPConf #3dprinting https://t.co/0Bdx3apBCf
3DPrint @3DPrint_com6:55 PM – 22 Oct 2015 to 33,757 followers 0 1

Getting underway now! Stay tuned for live updates on the #StartupCompetition at #I3DPConf https://t.co/uH2ZhHFUMI
Negi @nara02046:54 PM – 22 Oct 2015 to 249 followers 0 1
Printing a living tissue for Healthcare, #Fashion industry & education are 3 major trends to expect from #3dprinting! #3DPWeekCA #I3DPConf

Matthew Leta @MatthewLeta6:52 PM – 22 Oct 2015 to 32 followers 2 0

RT @Inside3DPrint: #I3DPConf #art session w/ @brad_rothenberg:How to affect material performance & properties by using #geometry https://t.…

Nicole (Belt) Cox @Tr1ckynikki6:48 PM – 22 Oct 2015 to 35 followers 0 0

#3DPrinting has a big place in the classroom and will get kids excited again about #manufacturing @stratasys #I3DPConf @3dpweek @TheUPSStore

SF Fashtech @sffashiontech6:46 PM – 22 Oct 2015 to 2,679 followers 1 0

RT @Inside3DPrint: #3dprinting extends to #ear #cartilage w/ Dr. James Yoo, @wakehealth, at #I3DPConf #medical track #bioprinting https://t…

AccountingSuite @AccountingSuite6:46 PM – 22 Oct 2015 to 594 followers 0 1

Terry Wohlers on the future of 3D Printing – We will be printing body replacement parts and food. #I3DPConf @inside3dprint

MecklerMedia @MecklerMedia6:45 PM – 22 Oct 2015 to 305 followers 0 1

Who will win $15K? #I3DPConf #Startup Competition starts NOW mcklr.me/1fpjkSI #3dprinting #AsimovVentures

Inside3DPrint @Inside3DPrint6:45 PM – 22 Oct 2015 to 3,143 followers 0 0

Who will win $15K? #I3DPConf #Startup Competition starts NOW mcklr.me/1fpjkSI #3dprinting #AsimovVentures

Nicole (Belt) Cox @Tr1ckynikki6:43 PM – 22 Oct 2015 to 35 followers 0 0

In the future, if you lose a finger you can #3dprint yourself a new one. #3dprinting living tissue is in the horizon. amazing! #I3DPConf

The UPS Store @TheUPSStore6:43 PM – 22 Oct 2015 to 11,688 followers 0 1
“Entrepreneurs can take their napkin ideas & actually bring them to life-it’s a great advantage for SBOs” @3d_printers #3DPWeek #I3DPConf

Local Motors @localmotors6:42 PM – 22 Oct 2015 to 9,325 followers 4 0

RT @TheUPSStore: “The industry has never been stronger!” @TerryWohlers at #I3DPConf #3DPWeek #3Dprinting

Inside3DPrint @Inside3DPrint6:42 PM – 22 Oct 2015 to 3,143 followers 1 0

#3dprinting extends to #ear #cartilage w/ Dr. James Yoo, @wakehealth, at #I3DPConf #medical track #bioprinting https://t.co/DU3sxtTMmy

Eric Nocella Diaz @ArgonautResins6:40 PM – 22 Oct 2015 to 1,577 followers 2 0

RT @SprintRay: In the Bay Area? Come visit our booth at @Inside3DPrint in Santa Clara this Wed & Thurs! #I3DPConf http://t.co/OMrRKhTGBr

Eric Nocella Diaz @ArgonautResins6:39 PM – 22 Oct 2015 to 1,577 followers 1 0

RT @SprintRay: We’re ready for day 2 of #I3DPConf Santa Clara! Come say hi & meet MoonRay. #3dprinting https://t.co/zB5L6ptCy3

Stewart Quealy @StewQ6:37 PM – 22 Oct 2015 to 8,204 followers 1 0
RT @christinatee: Final day #I3DPConf #3dprinting @MarkTrageser looking fwd to Future of Toys talk! https://t.co/w0AhO80WTq
3d print @Best3dprint6:37 PM – 22 Oct 2015 to 2,148 followers 0 0

@goengineer | #3dprint | We’re here at the #I3DPConf, Booth 315. Come by to see the #Objet… ift.tt/201bYbB https://t.co/Av3KoeOqp2

Stewart Quealy @StewQ6:36 PM – 22 Oct 2015 to 8,204 followers 1 0
RT @Tr1ckynikki: We’re seeing an explosion in schools adopting #3dprinting Encouraging news from the #I3DPConf @stratasys

Nicole (Belt) Cox @Tr1ckynikki6:35 PM – 22 Oct 2015 to 35 followers 1 0

We’re seeing an explosion in schools adopting #3dprinting Encouraging news from the #I3DPConf @stratasys

The UPS Store @TheUPSStore6:34 PM – 22 Oct 2015 to 11,688 followers 2 0
RT @Inside3DPrint: “The next frontier in #3dprinting is #manufacturing” @TerryWohlers, @TheUPSStore #I3DPConf #AskTheExperts

Chris DeCristofaro @cdecrist6:32 PM – 22 Oct 2015 to 116 followers 7 0
RT @3DPrintGirl: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t.…
GoEngineer @GoEngineer6:29 PM – 22 Oct 2015 to 1,689 followers 0 1

We’re here at the #I3DPConf Booth 315. Stop by to check out the #Objet30 Pro in action. Also featured: mold… fb.me/3XYOwjzwg

Hasti Afsarifard @kojaahasti6:27 PM – 22 Oct 2015 to 99 followers 0 3

Testing out an ultra light #3dprinted violin #I3DPConf https://t.co/KGArzUBVmZ

3D Print Week CA @3dprintweekca6:26 PM – 22 Oct 2015 to 81 followers 2 0

RT @TheUPSStore: The #i3DPConf Ask the Experts Panel w/ @TerryWohlers & Rich Garrity of #Stratasys @3d_printers has begun! #3DPWeek https:…

Inside3DPrint @Inside3DPrint6:25 PM – 22 Oct 2015 to 3,143 followers 2 0

RT @GatewayCDI: .@3DForged: At UPS Store Booth @ #I3DPConf. When UPS jumps into new technology you know it’s a big deal. #3dprinting https:…

Inside3DPrint @Inside3DPrint6:24 PM – 22 Oct 2015 to 3,143 followers 2 0

RT @PixelxVore: Here at #I3DPConf for #3DPWeek in Santa Clara with @TheUPSStore’s Ask The Experts panel. https://t.co/9Nh60vcADP
Aaron Levinson @aaronilevinson6:23 PM – 22 Oct 2015 to 57 followers 1 0

RT @Inside3DPrint: ANNOUNCED #I3DPConf: ‘The Nature Game’ insp. by #AlanTuring, Apr 2016. Can we create nature? mcklr.me/1NWqXzH htt…

Andrew Flando @AF_metalformag6:22 PM – 22 Oct 2015 to 98 followers 2 0

RT @Inside3DPrint: “The industry has never been stronger.” – @TerryWohlers during @TheUPSStore #AskTheExperts panel #3DPWeek #I3DPConf #3dp…

Inside3DPrint @Inside3DPrint6:22 PM – 22 Oct 2015 to 3,143 followers 0 1

The ‘Experts’ answer audience questions at #AskTheExperts panel #i3DPConf @TheUPSStore @TerryWohlers @Stratasys https://t.co/C0ES7eo8uJ

Linda Fung @PixelxVore6:21 PM – 22 Oct 2015 to 11 followers 2 5

Here at #I3DPConf for #3DPWeek in Santa Clara with @TheUPSStore’s Ask The Experts panel. https://t.co/9Nh60vcADP
Forecast3D @Forecast3D6:21 PM – 22 Oct 2015 to 172 followers 1 0
RT @aaronilevinson: Spolier, tail light lenses, quarter panel ext…all 3D printed by @Forecast3D! #I3DPConf https://t.co/EPwnuLxixI

Forecast3D @Forecast3D6:20 PM – 22 Oct 2015 to 172 followers 0 1
Good morning! Last day to catch us @ #I3DPConf in booth 309. Come see us, we’d love to chat & answer any #3dprinting questions! #3DThursday

The UPS Store @TheUPSStore6:14 PM – 22 Oct 2015 to 11,688 followers 4 5
“The industry has never been stronger!” @TerryWohlers at #I3DPConf #3DPWeek #3Dprinting

Inside3DPrint @Inside3DPrint6:13 PM – 22 Oct 2015 to 3,143 followers 2 0

“The next frontier in #3dprinting is #manufacturing” @TerryWohlers, @TheUPSStore #I3DPConf #AskTheExperts

Electric Runway @Electric_Runway6:13 PM – 22 Oct 2015 to 589 followers 6 0

RT @IntraconGlobal: #nTopology believes with a little development, #3Dprinted shirts like this are the future of your wardrobe #I3DPConf ht…

Scansite @Scansite3D6:13 PM – 22 Oct 2015 to 13 followers 0 2
We’re all set up! Come stop by our #I3DPConf booth. #3dscanning #3dprinting https://t.co/Cb8IAmgteX
3DXpressions @3DXpressions6:13 PM – 22 Oct 2015 to 348 followers 7 0

RT @brianfederal: Natacha Alpert speaks today @ Inside 3D Printing Santa Clara on #3DPrinting in #Fashion 1:30 #I3DPConf #3DPrinter https:/…

Nicole (Belt) Cox @Tr1ckynikki6:11 PM – 22 Oct 2015 to 35 followers 0 4

Behind the scenes look at @TheUPSStore Ask the Experts panel #3DPWeek #I3DPConf @Stratasys @TerryWohlers https://t.co/B09icHWESD

Inside3DPrint @Inside3DPrint6:10 PM – 22 Oct 2015 to 3,143 followers 0 1

Increase in adding #thermoplastic into mold inserts, “Shooting plastic through plastic”- Rich Garrity, @Stratasys @TheUPSStore #I3DPConf

GatewayCDI @GatewayCDI6:10 PM – 22 Oct 2015 to 320 followers 2 3

.@3DForged: At UPS Store Booth @ #I3DPConf. When UPS jumps into new technology you know it’s a big deal. #3dprinting https://t.co/CSJ1lcSx5L

The UPS Store @TheUPSStore6:10 PM – 22 Oct 2015 to 11,688 followers 2 4
The #i3DPConf Ask the Experts Panel w/ @TerryWohlers & Rich Garrity of #Stratasys @3d_printers has begun! #3DPWeek https://t.co/PHQ6jADbGe

GoEngineer @GoEngineer6:08 PM – 22 Oct 2015 to 1,689 followers 0 1

We’re here at the #I3DPConf, Booth 315. Come by to see the #Objet30 Pro in action! @stratasys… instagram.com/p/9Jj2rViso4/

DailyCADCAM.com @dailycadcam6:03 PM – 22 Oct 2015 to 4,288 followers 1 0

RT @Inside3DPrint: ..@TheUPSStore is celebrating #3dprinting during #3DPWeek to educate about the value of 3D printing, says Daniel Remba #…
Inside3DPrint @Inside3DPrint6:02 PM – 22 Oct 2015 to 3,143 followers 2 2

“The industry has never been stronger.” – @TerryWohlers during @TheUPSStore #AskTheExperts panel #3DPWeek #I3DPConf #3dprinting

Inside3DPrint @Inside3DPrint6:01 PM – 22 Oct 2015 to 3,143 followers 1 1

..@TheUPSStore is celebrating #3dprinting during #3DPWeek to educate about the value of 3D printing, says Daniel Remba #I3DPConf

Inside3DPrint @Inside3DPrint5:54 PM – 22 Oct 2015 to 3,143 followers 2 0

RT @TheUPSStore: We’re here! Check out the action behind the scenes at the #i3DPConf & submit your 3D printing questions w/ #3DPWeek! https…

Inside3DPrint @Inside3DPrint5:53 PM – 22 Oct 2015 to 3,143 followers 1 0

RT @3DPrint_com: Here we go! #AsktheExperts #I3DPConf #TheUPSStore #Wohlers #Stratasys https://t.co/JMKfXT2Qe8

Mara Hitner @3DPGirl5:53 PM – 22 Oct 2015 to 120 followers 0 2

Scott Summit killed it at #I3DPConf #keynote! @BespokeInc @3dsystemscorp Thank you for the inspiration! #enable #3dprinting #WeAreTheFuture

3D Print Week CA @3dprintweekca5:51 PM – 22 Oct 2015 to 81 followers 1 0

RT @Inside3DPrint: Grab your seat at @TheUPSStore’s #AskTheExperts w/ Daniel Remba, @TerryWohlers & Rich Garrity @Stratasys #I3DPConf https…

Inside3DPrint @Inside3DPrint5:50 PM – 22 Oct 2015 to 3,143 followers 1 2

Grab your seat at @TheUPSStore’s #AskTheExperts w/ Daniel Remba, @TerryWohlers & Rich Garrity @Stratasys #I3DPConf https://t.co/si9c35mPmH

Infinite3D Solutions @Infinite3ds5:50 PM – 22 Oct 2015 to 33 followers 1 0
RT @Inside3DPrint: Today for #networking passes: keynotes w/ @autodesk + @3dsystemscorp; @TheUPSStore session; #Startup Competition; & exhi…

3DPrint @3DPrint_com5:49 PM – 22 Oct 2015 to 33,757 followers 1 2

Here we go! #AsktheExperts #I3DPConf #TheUPSStore #Wohlers #Stratasys https://t.co/JMKfXT2Qe8
Inside3DPrint @Inside3DPrint5:45 PM – 22 Oct 2015 to 3,143 followers 0 0

The answers to your #3dprinting questions are here! @TheUPSStore Panel starts NOW mcklr.me/1LmSvbw @stratasys @TerryWohlers #I3DPConf

AccountingSuite @AccountingSuite5:41 PM – 22 Oct 2015 to 594 followers 0 1

Scott Summit – “Let’s take coke bottles and print prosthetic hands.” #I3DPConf @inside3dprint

Infinite3D Solutions @Infinite3ds5:38 PM – 22 Oct 2015 to 33 followers 0 0
Follow us on SnapChat @ i3dprint #I3DPConf instagram.com/p/9JgatEHXwg/

Mark Trageser @MarkTrageser5:38 PM – 22 Oct 2015 to 48 followers 0 0

#insaniTOY #i3dpconf wood rocket instagram.com/p/9JgYZirSDL/

Mark Trageser @MarkTrageser5:36 PM – 22 Oct 2015 to 48 followers 0 0

#i3dpconf #insaniTOY vase explosion BOOM instagram.com/p/9JgOs3LSCy/

Mark Trageser @MarkTrageser5:35 PM – 22 Oct 2015 to 48 followers 0 0

#insaniTOY #i3dpconf cheetahs instagram.com/p/9JgFNeLSCW/

la moustache ringard @SJGrunewald5:35 PM – 22 Oct 2015 to 234 followers 0 1

Looking forward to the Toy Design & Beyond panel this morning. #3dprinting is changing every industry! #I3DPConf

GatewayCDI @GatewayCDI5:35 PM – 22 Oct 2015 to 320 followers 0 1

.@3DForged: Print, scan, fax? The @AIORobotics Zeus does it all. They might be the coolest guys at #I3DPConf too. https://t.co/3esvKyUjVn

Mark Trageser @MarkTrageser5:34 PM – 22 Oct 2015 to 48 followers 0 0

#i3dpconf #insaniTOY #hoganas… They are hard to see because they are sooooo beautifully small.… instagram.com/p/9Jf_zyLSCK/

Inside3DPrint @Inside3DPrint5:34 PM – 22 Oct 2015 to 3,143 followers 0 0

Exhibit hall closes at 3:45! Exhibitors like @FilamentExpress are excited to share their creations w/ you #I3DPConf https://t.co/pd8T7z0xqx

3DPrintPress @3DPrintPress5:31 PM – 22 Oct 2015 to 194 followers 1 0

RT @Inside3DPrint: Scott Summit opts 4 #3dprinted brace over plaster cast for arm injury. #3dprinting is truly the future of #medicine @Bes…

3DPrintPress @3DPrintPress5:30 PM – 22 Oct 2015 to 194 followers 1 0

RT @Inside3DPrint: #3dprinted equip w/ patient’s #fashion preferences leads to more usage & faster recovery. Scott Summit @BespokeInc @3dsy…

3DPrintPress @3DPrintPress5:29 PM – 22 Oct 2015 to 194 followers 0 1

Made in Space Keynote Examines AM Among the Stars @ #I3DPConf 3dprintpress.org https://t.co/ibHWe8CC2P
christine trageser @christinatee5:26 PM – 22 Oct 2015 to 203 followers 1 0

RT @MarkTrageser: #insaniTOY #i3dpconf spiky fashion. Wood bracelets. instagram.com/p/9JewR3rSPm/

Mark Trageser @MarkTrageser5:24 PM – 22 Oct 2015 to 48 followers 1 1

#insaniTOY #i3dpconf spiky fashion. Wood bracelets. instagram.com/p/9JewR3rSPm/

Your Accounting Dept @WatkinSBS5:23 PM – 22 Oct 2015 to 438 followers 2 0

RT @TheUPSStore: We’re here! Check out the action behind the scenes at the #i3DPConf & submit your 3D printing questions w/ #3DPWeek! https…

Tech XB @TechXB5:23 PM – 22 Oct 2015 to 3,394 followers 6 0

RT @Inside3DPrint: We have a packed last day of #I3DPConf Santa Clara! Registration open now & exhibit hall at 10AM mcklr.me/1LQzGD2…

The UPS Store @TheUPSStore5:23 PM – 22 Oct 2015 to 11,688 followers 2 4
We’re here! Check out the action behind the scenes at the #i3DPConf & submit your 3D printing questions w/ #3DPWeek! https://t.co/ReekAquM3U

Inside3DPrint @Inside3DPrint5:23 PM – 22 Oct 2015 to 3,143 followers 1 1

#3dprinted equip w/ patient’s #fashion preferences leads to more usage & faster recovery. Scott Summit @BespokeInc @3dsystemscorp #I3DPConf

alex@fabbing @fabbing5:16 PM – 22 Oct 2015 to 8,565 followers 1 0

RT @brianfederal: Happening now Scott Summit Keynote #I3DPConf Fantastic! #3DPrinting

Stewart Quealy @StewQ5:15 PM – 22 Oct 2015 to 8,204 followers 1 0
RT @brianfederal: Stewart Quealy and Team Meckler put on another amazing event @ Inside 3D Printing Santa Clara #3DPrinting #I3DPConf https…

Stewart Quealy @StewQ5:14 PM – 22 Oct 2015 to 8,204 followers 6 0
RT @Inside3DPrint: We have a packed last day of #I3DPConf Santa Clara! Registration open now & exhibit hall at 10AM mcklr.me/1LQzGD2…

Inside3DPrint @Inside3DPrint5:13 PM – 22 Oct 2015 to 3,143 followers 1 0

Scott Summit opts 4 #3dprinted brace over plaster cast for arm injury. #3dprinting is truly the future of #medicine @BespokeInc #I3DPConf

SprintRay @SprintRay5:12 PM – 22 Oct 2015 to 347 followers 1 3

We’re ready for day 2 of #I3DPConf Santa Clara! Come say hi & meet MoonRay. #3dprinting https://t.co/zB5L6ptCy3

Tyler Benster @tbenst5:07 PM – 22 Oct 2015 to 454 followers 1 0
RT @3Dprintbeginner: Catch @tbenst at @Inside3DPrint #I3DPConf Shanghai, Dec 8-10 mcklr.me/1UnTfkV #3dprinting

Intracon @IntraconGlobal5:06 PM – 22 Oct 2015 to 116 followers 1 1

With #thenaturegame, they are inviting creators to attempt to recreate organic complexity with #3Dprinting. #I3DPConf

christine trageser @christinatee5:01 PM – 22 Oct 2015 to 203 followers 1 1

Final day #I3DPConf #3dprinting @MarkTrageser looking fwd to Future of Toys talk! https://t.co/w0AhO80WTq
Inside3DPrint @Inside3DPrint5:00 PM – 22 Oct 2015 to 3,143 followers 1 1

ANNOUNCED #I3DPConf: ‘The Nature Game’ insp. by #AlanTuring, Apr 2016. Can we create nature? mcklr.me/1NWqXzH https://t.co/p5BB6CztON

Brian Federal @brianfederal5:00 PM – 22 Oct 2015 to 10,290 followers 1 1

Happening now Scott Summit Keynote #I3DPConf Fantastic! #3DPrinting

Inside3DPrint @Inside3DPrint5:00 PM – 22 Oct 2015 to 3,143 followers 0 0

11AM: #Metal: @Kunal_S_Varma of @EFESTOLLC; #Art: @MarkTrageser of Kram-Co mcklr.me/1GIOZWQ #I3DPConf https://t.co/cWaPBZflV1

Aaron Levinson @aaronilevinson4:59 PM – 22 Oct 2015 to 57 followers 3 0

RT @MarkTrageser: #I3dpconf #insaniTOY. Santa Clara expo https://t.co/uS3MDvMgTF

Aaron Levinson @aaronilevinson4:59 PM – 22 Oct 2015 to 57 followers 1 0

RT @MarkTrageser: #I3DPConf #insaniTOY light explosion https://t.co/TSyVwEJGxu

Inside3DPrint @Inside3DPrint4:58 PM – 22 Oct 2015 to 3,143 followers 6 0

RT @IntraconGlobal: #nTopology believes with a little development, #3Dprinted shirts like this are the future of your wardrobe #I3DPConf ht…

Mark Trageser @MarkTrageser4:58 PM – 22 Oct 2015 to 48 followers 1 0

#I3DPConf #insaniTOY light explosion https://t.co/TSyVwEJGxu

Inside3DPrint @Inside3DPrint4:58 PM – 22 Oct 2015 to 3,143 followers 3 0

RT @MarkTrageser: #I3dpconf #insaniTOY. Santa Clara expo https://t.co/uS3MDvMgTF

Fashion Project BHS @FashionProj4:57 PM – 22 Oct 2015 to 155 followers 6 0

RT @IntraconGlobal: #nTopology believes with a little development, #3Dprinted shirts like this are the future of your wardrobe #I3DPConf ht…

Scansite @Scansite3D4:56 PM – 22 Oct 2015 to 13 followers 0 1
Setting up for day 2 at #I3DPConf. Here are some pics from day 1 #3dprinting #3dscanning https://t.co/YamBX7g3wo
Mark Trageser @MarkTrageser4:56 PM – 22 Oct 2015 to 48 followers 3 1

#I3dpconf #insaniTOY. Santa Clara expo https://t.co/uS3MDvMgTF

SF Fashtech @sffashiontech4:52 PM – 22 Oct 2015 to 2,679 followers 6 0

RT @IntraconGlobal: #nTopology believes with a little development, #3Dprinted shirts like this are the future of your wardrobe #I3DPConf ht…

BradleyRothenberg @brad_rothenberg4:51 PM – 22 Oct 2015 to 338 followers 2 0

RT @Inside3DPrint: #I3DPConf #art session w/ @brad_rothenberg:How to affect material performance & properties by using #geometry https://t.…

Inside3DPrint @Inside3DPrint4:45 PM – 22 Oct 2015 to 3,143 followers 0 0

#I3DPConf MORNING KEYNOTE: ‘#3DPrinting Meets Human Needs’ ft Scott Summit, @3dsystemscorp mcklr.me/1MJnJNv https://t.co/KwAwX2aFZX

Intracon @IntraconGlobal4:43 PM – 22 Oct 2015 to 116 followers 6 4

#nTopology believes with a little development, #3Dprinted shirts like this are the future of your wardrobe #I3DPConf https://t.co/ilBQaiFC5H

Inside3DPrint @Inside3DPrint4:40 PM – 22 Oct 2015 to 3,143 followers 1 0

RT @StewQ: Bryan Dow of @MoorelandGlobal talking Additive Manufacturing M&A and Financing Trends. #I3DPConf https://t.co/YMcH62Z72k

Intracon @IntraconGlobal4:38 PM – 22 Oct 2015 to 116 followers 0 1

It’s the final day of #I3DPConf! But there’s still lots of amazing #3Dprinting technology happening, let’s get into it. 💪👏
la moustache ringard @SJGrunewald4:38 PM – 22 Oct 2015 to 234 followers 0 1

Inside the Inside #3DPrinting Conference and Expo Santa Clara: Part One 3dprint.com/101899/i3dpcon… #I3DPConf https://t.co/GMaEBCuGuO

Stewart Quealy @StewQ4:37 PM – 22 Oct 2015 to 8,204 followers 1 1
Bryan Dow of @MoorelandGlobal talking Additive Manufacturing M&A and Financing Trends. #I3DPConf https://t.co/YMcH62Z72k

Inside3DPrint @Inside3DPrint4:36 PM – 22 Oct 2015 to 3,143 followers 2 2

#I3DPConf #art session w/ @brad_rothenberg:How to affect material performance & properties by using #geometry https://t.co/mFJYp3sLup

Patrick O’Neil @Padsuk4:32 PM – 22 Oct 2015 to 7,730 followers 4 0
RT @3DPrintingIt: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t…
Andrew Flando @AF_metalformag4:32 PM – 22 Oct 2015 to 98 followers 2 0

RT @Inside3DPrint: .@winsheldon of SLM Solutions: impact of disruptive forces at the moment is limited #I3DPConf #3dprinting #metal https:/…

3DMetalPrintMag @3DMetalPrintMag4:31 PM – 22 Oct 2015 to 40 followers 2 0

RT @Inside3DPrint: .@winsheldon of SLM Solutions: impact of disruptive forces at the moment is limited #I3DPConf #3dprinting #metal https:/…

Inside3DPrint @Inside3DPrint4:31 PM – 22 Oct 2015 to 3,143 followers 1 0

Today for #networking passes: keynotes w/ @autodesk + @3dsystemscorp; @TheUPSStore session; #Startup Competition; & exhibits #I3DPConf

Tony Conaway @TonyConaway4:26 PM – 22 Oct 2015 to 542 followers 7 0

RT @3DPrintGirl: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t.…
3D Print Week CA @3dprintweekca4:25 PM – 22 Oct 2015 to 81 followers 2 0

RT @sffashiontech: Last night’s #3dprinting @Autodesk event is a taste of the future in fashion. Follow ongoing innovation at @Inside3DPrin…

Inside3DPrint @Inside3DPrint4:25 PM – 22 Oct 2015 to 3,143 followers 2 0

RT @sffashiontech: Last night’s #3dprinting @Autodesk event is a taste of the future in fashion. Follow ongoing innovation at @Inside3DPrin…

Inside3DPrint @Inside3DPrint4:25 PM – 22 Oct 2015 to 3,143 followers 2 0

.@winsheldon of SLM Solutions: impact of disruptive forces at the moment is limited #I3DPConf #3dprinting #metal https://t.co/5gpQ4OZLdY

SF Fashtech @sffashiontech4:24 PM – 22 Oct 2015 to 2,679 followers 2 2

Last night’s #3dprinting @Autodesk event is a taste of the future in fashion. Follow ongoing innovation at @Inside3DPrint! #I3DPConf

Inside3DPrint @Inside3DPrint4:23 PM – 22 Oct 2015 to 3,143 followers 2 1

.@winsheldon of SLM Solutions: impact of disruptive forces at the moment is limited #I3DPConf #3dprinting #metal https://t.co/dGX8Q0YhBn

Inside3DPrint @Inside3DPrint4:20 PM – 22 Oct 2015 to 3,143 followers 2 0

RT @BunnyEllerin: Amazing presentation by Jeff Huber @StandardCyborg on personalized manufacturing of prosthetics #I3DPConf https://t.co/z6…

Bunny Ellerin @BunnyEllerin4:13 PM – 22 Oct 2015 to 2,450 followers 2 1

Amazing presentation by Jeff Huber @StandardCyborg on personalized manufacturing of prosthetics #I3DPConf https://t.co/z6FM0vDB2Y

la moustache ringard @SJGrunewald4:06 PM – 22 Oct 2015 to 234 followers 0 1

Inside 3D Printing Conference: Are We #3DPrinting a Copyright Free World? 3dprint.com/101891/3d-prin… #I3DPConf https://t.co/C8gOF01ueN

Tommy Keiser @TommyKeiser3:53 PM – 22 Oct 2015 to 223 followers 6 0

RT @Inside3DPrint: We have a packed last day of #I3DPConf Santa Clara! Registration open now & exhibit hall at 10AM mcklr.me/1LQzGD2…

Start 3D Printing @3Dprintbeginner3:51 PM – 22 Oct 2015 to 2,989 followers 1 2

Catch @tbenst at @Inside3DPrint #I3DPConf Shanghai, Dec 8-10 mcklr.me/1UnTfkV #3dprinting

Bazooka Tech @bazooka_tech3:50 PM – 22 Oct 2015 to 1,173 followers 6 0
RT @Inside3DPrint: We have a packed last day of #I3DPConf Santa Clara! Registration open now & exhibit hall at 10AM mcklr.me/1LQzGD2…

Brian Federal @brianfederal3:47 PM – 22 Oct 2015 to 10,290 followers 2 0

RT @IntraconGlobal: From inoperable to operable: hearing from @Stratasys about the medical applications of #3dprinting. #I3DPConf https://t…

Brian Federal @brianfederal3:47 PM – 22 Oct 2015 to 10,290 followers 6 0

RT @Inside3DPrint: We have a packed last day of #I3DPConf Santa Clara! Registration open now & exhibit hall at 10AM mcklr.me/1LQzGD2…

alex@fabbing @fabbing3:46 PM – 22 Oct 2015 to 8,565 followers 1 0

RT @brianfederal: #Lulzbot is a great choice for #3DPrinting in Education.Open Source teaches problem solving #I3DPConf #3DPrinters https:/…

Oskar Ramajo @OskarRamajo3:44 PM – 22 Oct 2015 to 551 followers 7 0

RT @brianfederal: Natacha Alpert speaks today @ Inside 3D Printing Santa Clara on #3DPrinting in #Fashion 1:30 #I3DPConf #3DPrinter https:/…

Peter Westerholm @peter29ner3:42 PM – 22 Oct 2015 to 585 followers 3 0
RT @Inside3DPrint: Check out @Forecast3D’s #3dprint car w/ drink in hand at the #networking reception until 6pm! #I3DPConf https://t.co/LuX…

3DMetalPrintMag @3DMetalPrintMag3:39 PM – 22 Oct 2015 to 40 followers 6 0

RT @Inside3DPrint: We have a packed last day of #I3DPConf Santa Clara! Registration open now & exhibit hall at 10AM mcklr.me/1LQzGD2…

3DMetalPrintMag @3DMetalPrintMag3:38 PM – 22 Oct 2015 to 40 followers 5 0

RT @GARTEL: Great day @ #I3DPConf, #CreativeForce #Design Competition in CA this week #3dprinting #gartel3d #makeprintable https://t.co/kq…

Inside3DPrint @Inside3DPrint3:37 PM – 22 Oct 2015 to 3,143 followers 2 0

RT @IntraconGlobal: From inoperable to operable: hearing from @Stratasys about the medical applications of #3dprinting. #I3DPConf https://t…

Brian Federal @brianfederal3:37 PM – 22 Oct 2015 to 10,290 followers 1 1

#Lulzbot is a great choice for #3DPrinting in Education.Open Source teaches problem solving #I3DPConf #3DPrinters https://t.co/gyCWvUt0C2

Lauren Wallace @SavvyLaur3:36 PM – 22 Oct 2015 to 99 followers 3 0

RT @SavvySoc: Don’t miss @SavvyAlexa telling our story today @Inside3DPrint!! #StartupCompetition #I3DPConf #3dpfashion #womenintech #STEM

Inside3DPrint @Inside3DPrint3:36 PM – 22 Oct 2015 to 3,143 followers 5 0

RT @GARTEL: Great day @ #I3DPConf, #CreativeForce #Design Competition in CA this week #3dprinting #gartel3d #makeprintable https://t.co/kq…

Inside3DPrint @Inside3DPrint3:34 PM – 22 Oct 2015 to 3,143 followers 1 0

RT @brianfederal: Learn about #3DPrinting concrete today @ Inside 3D Printing Santa Clara with Tony Capasso 3:30 PM #I3DPConf https://t.co/…

Inside3DPrint @Inside3DPrint3:33 PM – 22 Oct 2015 to 3,143 followers 1 0

RT @3DPrint_com: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t.…
Inside3DPrint @Inside3DPrint3:31 PM – 22 Oct 2015 to 3,143 followers 0 0

All #I3DPConf Santa Clara attendees have access to this session! Get there early as it’s first come, first serve. twitter.com/TheUPSStore/st…

Simonetta Pegorari @compositemood3:20 PM – 22 Oct 2015 to 215 followers 2 0
RT @3DNewsNow: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t.co…
Brian Federal @brianfederal3:19 PM – 22 Oct 2015 to 10,290 followers 0 0

Do not miss #3DPrinting Evangelist Tyler Benster today for a glimpse of the future @ Inside 3D Printing Santa Clara 4:15 #I3DPConf

Inside3DPrint @Inside3DPrint3:15 PM – 22 Oct 2015 to 3,143 followers 6 2

We have a packed last day of #I3DPConf Santa Clara! Registration open now & exhibit hall at 10AM mcklr.me/1LQzGD2 #3dprinting

NIHL 3D Printing @NIHL3D3:09 PM – 22 Oct 2015 to 228 followers 2 0
RT @My3DPrinting: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t…
Gina O’Connell @gina_oconnell3:08 PM – 22 Oct 2015 to 807 followers 4 0

RT @3DPrintingIt: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t…
Brian Federal @brianfederal3:06 PM – 22 Oct 2015 to 10,290 followers 1 5

Stewart Quealy and Team Meckler put on another amazing event @ Inside 3D Printing Santa Clara #3DPrinting #I3DPConf https://t.co/8YMwoIR9I4

Penny Ann Dolin @Pennyd3:02 PM – 22 Oct 2015 to 951 followers 6 0
RT @3DPrintGirl: The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/pHo7wYeUQY

o3dprint.ru @o3dprintru3:01 PM – 22 Oct 2015 to 2,600 followers 7 0

RT @brianfederal: Natacha Alpert speaks today @ Inside 3D Printing Santa Clara on #3DPrinting in #Fashion 1:30 #I3DPConf #3DPrinter https:/…

Angela Cummins @angcummins2:56 PM – 22 Oct 2015 to 392 followers 2 0

RT @Inside3DPrint: Crowded room for @HuschBlackwell’s sponsored session at #I3DPConf Santa Clara #3dprinting https://t.co/CaCU7kYBWy

El VooDoo Daddy @BSUPimp2:55 PM – 22 Oct 2015 to 1,541 followers 2 0

RT @My3DPrinting: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t…
Tara McNulty @mcnulty_tara2:54 PM – 22 Oct 2015 to 218 followers 3 0

RT @SavvySoc: Don’t miss @SavvyAlexa telling our story today @Inside3DPrint!! #StartupCompetition #I3DPConf #3dpfashion #womenintech #STEM

cempohualxochitl @buhomilhojas2:52 PM – 22 Oct 2015 to 218 followers 4 0

RT @3DPrintingIt: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t…
Shoot @getshootapp2:48 PM – 22 Oct 2015 to 1,506 followers 3 0
RT @SavvySoc: Don’t miss @SavvyAlexa telling our story today @Inside3DPrint!! #StartupCompetition #I3DPConf #3dpfashion #womenintech #STEM

Savvy Society @SavvySoc2:47 PM – 22 Oct 2015 to 99 followers 3 0
Don’t miss @SavvyAlexa telling our story today @Inside3DPrint!! #StartupCompetition #I3DPConf #3dpfashion #womenintech #STEM

alex@fabbing @fabbing2:45 PM – 22 Oct 2015 to 8,565 followers 7 0

RT @brianfederal: Natacha Alpert speaks today @ Inside 3D Printing Santa Clara on #3DPrinting in #Fashion 1:30 #I3DPConf #3DPrinter https:/…

alex@fabbing @fabbing2:44 PM – 22 Oct 2015 to 8,565 followers 3 0

RT @3DPrintBoard: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t…
3D Printing Info ™ @Info3DPrinting2:43 PM – 22 Oct 2015 to 115 followers 3 0

RT @3DPrintBoard: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t…
Thomas @ellisonthomas2:41 PM – 22 Oct 2015 to 291 followers 2 0

RT @3DNewsNow: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t.co…
3D Printing @3D_Printing_it2:40 PM – 22 Oct 2015 to 2,209 followers 0 0

Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world 3dprint.com/101891/3d-prin…

3D Printing News @My3DPrinting2:40 PM – 22 Oct 2015 to 54,848 followers 2 1

Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world 3dprint.com/101891/3d-prin…

3D Printing News @3DNewsNow2:40 PM – 22 Oct 2015 to 3,314 followers 2 0
Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world 3dprint.com/101891/3d-prin…

3D Print .com @3DPrintBoard2:40 PM – 22 Oct 2015 to 46,499 followers 3 2

Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world 3dprint.com/101891/3d-prin…

3DPrint @3DPrint_com2:40 PM – 22 Oct 2015 to 33,757 followers 1 1

Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world 3dprint.com/101891/3d-prin…

LemonMaker @lemon_maker2:36 PM – 22 Oct 2015 to 1,767 followers 7 0

RT @brianfederal: Natacha Alpert speaks today @ Inside 3D Printing Santa Clara on #3DPrinting in #Fashion 1:30 #I3DPConf #3DPrinter https:/…

Brian Federal @brianfederal2:36 PM – 22 Oct 2015 to 10,290 followers 7 3

Natacha Alpert speaks today @ Inside 3D Printing Santa Clara on #3DPrinting in #Fashion 1:30 #I3DPConf #3DPrinter https://t.co/zy456YP449

Bazooka Tech @bazooka_tech2:34 PM – 22 Oct 2015 to 1,173 followers 4 0
RT @3DPrintingIt: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t…
3DMetalPrintMag @3DMetalPrintMag2:32 PM – 22 Oct 2015 to 40 followers 7 0

RT @3DPrintGirl: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https://t.…
Brian Federal @brianfederal2:28 PM – 22 Oct 2015 to 10,290 followers 1 1

Learn about #3DPrinting concrete today @ Inside 3D Printing Santa Clara with Tony Capasso 3:30 PM #I3DPConf https://t.co/viG1PJ3qFM

3D Printer News @3DPrinterNews2:28 PM – 22 Oct 2015 to 1,078 followers 0 0

twitter.com/3DPrintBoardco… Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilitie… https://t.co/6RXXYbu6yo

N Greg Ford @NGregFord2:24 PM – 22 Oct 2015 to 55 followers 2 0

RT @3DPrintingFans: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https:/…
カモノハシ @roso19542:23 PM – 22 Oct 2015 to 9,915 followers 0 0
＠3DPrintGirl: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in… 3dprint.com/101891/3d-prin…

MaG @HumanGrip2:19 PM – 22 Oct 2015 to 1,664 followers 2 0

RT @3DPrintingFans: Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world https:/…
3D Printing Fans @3DPrintingFans2:17 PM – 22 Oct 2015 to 17,194 followers 2 1

Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world 3dprint.com/101891/3d-prin…

3D Printing @3DPrintBoardcom2:17 PM – 22 Oct 2015 to 11,478 followers 0 0

Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world 3dprint.com/101891/3d-prin…

3D Printing @3D_Print_News2:17 PM – 22 Oct 2015 to 12,523 followers 4 4

Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world 3dprint.com/101891/3d-prin…

3D Printing @3DPrintingIt2:17 PM – 22 Oct 2015 to 15,317 followers 4 5

Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world 3dprint.com/101891/3d-prin…

3D Printing News @3DPrintGirl2:17 PM – 22 Oct 2015 to 40,617 followers 7 5

Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world 3dprint.com/101891/3d-prin…

The UPS Store @TheUPSStore2:00 PM – 22 Oct 2015 to 11,688 followers 0 1
Last chance to submit questions for our panel of experts at #I3DPConf! Use #3DPWeek! pic.twitter.com/jm7FDgtCde

NYC Health Business @NYCHBL1:52 PM – 22 Oct 2015 to 1,655 followers 1 0
RT @Inside3DPrint: Thought-provoking & inspirational day 1 #medical track curated by @BunnyEllerin, @NYCHBL! Join us tomorrow for day 2. #I…

Nicole (Belt) Cox @Tr1ckynikki1:40 PM – 22 Oct 2015 to 35 followers 0 1

In the Bay Area for work ready to geek out at the #I3DPConf today! Check out our booth! https://t.co/ksXOGF5RoF
Salman Qadir @iamGodgifted1:35 PM – 22 Oct 2015 to 1,045 followers 3 0

RT @3DPrintingIt: The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/notI7vBa0k

3-D Print Factory @3_dprintfactory1:30 PM – 22 Oct 2015 to 57 followers 0 0

@FaceOffSyfy If your folks are at #I3DPConf, come on by and see the Avatarium! Think ‘3D printed sculptures of your winning designs’!
3-D Print Factory @3_dprintfactory1:16 PM – 22 Oct 2015 to 57 followers 0 0

@3DPrintFactory at #I3DPConf, say hello if you’re in Santa Clara, & learn about the new Avatarium 3D Capture array! https://t.co/ZcSkRp5rWm
GoEngineer @GoEngineer1:15 PM – 22 Oct 2015 to 1,689 followers 1 0

RT @seancrotty: Good #3Dprinting survey & predictive talk by Tyler Reid of @GoEngineer at #I3DPConf . Slides here: youtube.com/watch?v=3ZUQ4Q…

3-D Print Factory @3_dprintfactory1:14 PM – 22 Oct 2015 to 57 followers 0 0

Heavy… today, Back to the Future becomes a movie about the past. Come visit us at the #I3DPConf & enjoy some future tech! @3DPrintFactory

spaceclaim @spaceclaim1:00 PM – 22 Oct 2015 to 2,661 followers 1 0

RT @aaronilevinson: This MASSIVE Perplexus puzzle was developed with @spaceclaim software. I need one for my house! #I3DPConf https://t.co/…

3DPrintingStocks.com @3DPrintingStock12:04 PM – 22 Oct 2015 to 2,090 followers 4 0

RT @brianfederal: Check out Scott Summit Inside 3D Printing Santa Clara 9:45 AM morning Keynote #I3DPConf #3DPrinting https://t.co/tFeCmJnw…

Margaret @hatzbymm11:59 AM – 22 Oct 2015 to 1,095 followers 3 0

RT @3DPrintingIt: The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/notI7vBa0k

Cristian P @Cristrack11:33 AM – 22 Oct 2015 to 223 followers 6 0
RT @3DPrintGirl: The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/pHo7wYeUQY

C.R.S. @ribeiroharman9:37 AM – 22 Oct 2015 to 1,562 followers 1 0

RT @3DPrint_com: The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/Ztg5ZpUYww

mikebit @77bergkamp199:34 AM – 22 Oct 2015 to 580 followers 1 0

RT @Inside3DPrint: Stay in the conversation! Use ‘#I3DPConf’ to share your thoughts on the Santa Clara show https://t.co/JtpAlXvHLx
mikebit @77bergkamp199:21 AM – 22 Oct 2015 to 580 followers 4 0

RT @3DPrintGirl: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https:/…

3DPrinterOS @3DPrinterOS7:50 AM – 22 Oct 2015 to 1,794 followers 2 0
RT @Inside3DPrint: “Current #3dprinting is like computers 40 yrs ago” @JDogru, @3DPrinterOS #I3DPConf https://t.co/gp29z9515H

IRiS 3D Solutions @iris3dsolutions7:22 AM – 22 Oct 2015 to 4,649 followers 4 0

RT @brianfederal: Check out Scott Summit Inside 3D Printing Santa Clara 9:45 AM morning Keynote #I3DPConf #3DPrinting https://t.co/tFeCmJnw…

IRiS 3D Solutions @iris3dsolutions7:18 AM – 22 Oct 2015 to 4,649 followers 5 0

RT @My3DPrinting: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/Oq…

IRiS 3D Solutions @iris3dsolutions7:17 AM – 22 Oct 2015 to 4,649 followers 2 0

RT @My3DPrinting: The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/97kfy1BZ8E

Materialise @MaterialiseNV6:50 AM – 22 Oct 2015 to 8,165 followers 4 0

RT @Inside3DPrint: Todd Pietila & Jon Detterich: #3dprinted #hearts give sick babies 2nd chances @MaterialiseNV @ChildrensLA #I3DPConf http…

Lluís Ariño @larinyo6:31 AM – 22 Oct 2015 to 143 followers 1 0

RT @3DPrintingFans: The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/uTqZF2VerB

Edward von Flottwell @flottwell5:51 AM – 22 Oct 2015 to 28 followers 4 0

RT @Inside3DPrint: Keynote from Rich Garrity, @Stratasys on the future of #additivemanufacturing is starting now! #I3DPConf #3dprinting htt…

michele merli @1mago55:13 AM – 22 Oct 2015 to 233 followers 4 0
RT @3DPrintGirl: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/ifG…

michele merli @1mago55:05 AM – 22 Oct 2015 to 233 followers 6 0
RT @3DPrintGirl: The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/pHo7wYeUQY

Bazooka Tech @bazooka_tech4:32 AM – 22 Oct 2015 to 1,173 followers 4 0
RT @brianfederal: Check out Scott Summit Inside 3D Printing Santa Clara 9:45 AM morning Keynote #I3DPConf #3DPrinting https://t.co/tFeCmJnw…

alex@fabbing @fabbing4:10 AM – 22 Oct 2015 to 8,565 followers 4 0

RT @brianfederal: Check out Scott Summit Inside 3D Printing Santa Clara 9:45 AM morning Keynote #I3DPConf #3DPrinting https://t.co/tFeCmJnw…

Enterprise in Space @EnterpriseSat3:49 AM – 22 Oct 2015 to 347 followers 2 0

RT @Stratasys: MT @Inside3DPrint: Keynote from Rich Garrity on future of #additivemanufacturing starts now! #I3DPConf #3dprinting https://t…

Brian Federal @brianfederal3:39 AM – 22 Oct 2015 to 10,290 followers 4 1

Check out Scott Summit Inside 3D Printing Santa Clara 9:45 AM morning Keynote #I3DPConf #3DPrinting https://t.co/tFeCmJnwQg

Roy Sherrill @myrddinstarhawk3:38 AM – 22 Oct 2015 to 146 followers 0 1

the VR summit at #I3DPConf @Inside3DPrint @Myrddinstarhawk for thenewsbubble.com facebook.com/thenewsbubble bubl.io/experiences/0b…

Roy Sherrill @myrddinstarhawk3:36 AM – 22 Oct 2015 to 146 followers 0 0

#I3DPConf @Inside3DPrint @Myrddinstarhawk for thenewsbubble.com facebook.com/thenewsbubble bubl.io/experiences/35…

Roy Sherrill @myrddinstarhawk3:33 AM – 22 Oct 2015 to 146 followers 0 0

#I3DPConf @Inside3DPrint @Myrddinstarhawk for thenewsbubble.com facebook.com/thenewsbubble . bubl.io/experiences/af…

InfraTrac @InfraTrac3:15 AM – 22 Oct 2015 to 68 followers 0 0

Amazing @Scansite3D at #I3DPConf replicates #dinosaurs, Michaelangelo #sculptures, #Ferrari vintage race cars: perfect copy 3D #scanning

Laurence Gartel @GARTEL3:12 AM – 22 Oct 2015 to 558 followers 5 7

Great day @ #I3DPConf, #CreativeForce #Design Competition in CA this week #3dprinting #gartel3d #makeprintable https://t.co/kqrdj7wF46

InfraTrac @InfraTrac3:07 AM – 22 Oct 2015 to 68 followers 0 0

#I3DPConf future panel: #3dprinting needs more advanced materials (hey, @Chemcubed) and better IP protection: @InfraTrac chemical layer

WYNIT 3D @WYNIT3D3:06 AM – 22 Oct 2015 to 259 followers 0 1

Had a great first day at #I3DPConf @Leapfrog_3D Follow us on Periscope for a live broadcast @Inside3DPrint https://t.co/9VGvh9myuj

Jay Simon @JaySimon19743:03 AM – 22 Oct 2015 to 2 followers 3 0

RT @WYNIT3D: Setting up the booth for @Inside3DPrint #3dprinting #i3dpconf Come by and see us tomorrow and Thursday! @Leapfrog_3D https://t…

3D Printer News @3DPrinterNews2:57 AM – 22 Oct 2015 to 1,078 followers 0 0

twitter.com/3DPrintBoardco… The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf … https://t.co/4KEaCt12gM

カモノハシ @roso19542:52 AM – 22 Oct 2015 to 9,915 followers 0 0
＠3DPrintGirl: The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/M0zdtzLYEi

alex@fabbing @fabbing2:49 AM – 22 Oct 2015 to 8,565 followers 1 0

RT @3DPrintBoard: The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/MaGh6nhGF8

Bazooka Tech @bazooka_tech2:48 AM – 22 Oct 2015 to 1,173 followers 3 0
RT @3DPrintingIt: The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/notI7vBa0k

HomeZilla @homezillaorguk2:47 AM – 22 Oct 2015 to 821 followers 2 0

RT @My3DPrinting: The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/97kfy1BZ8E

3D Printing @3DPrintBoardcom2:46 AM – 22 Oct 2015 to 11,478 followers 0 0

The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/tzUtg23o7d

3D Printing News @My3DPrinting2:46 AM – 22 Oct 2015 to 54,848 followers 2 0

The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/97kfy1BZ8E

3D Printing @3DPrintingIt2:46 AM – 22 Oct 2015 to 15,317 followers 3 2

The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/notI7vBa0k

3D Printing News @3DPrintGirl2:46 AM – 22 Oct 2015 to 40,617 followers 6 7

The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/pHo7wYeUQY

3D Print .com @3DPrintBoard2:46 AM – 22 Oct 2015 to 46,499 followers 1 0

The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/MaGh6nhGF8

3DPrint @3DPrint_com2:46 AM – 22 Oct 2015 to 33,757 followers 1 3

The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/Ztg5ZpUYww

3D Printing Fans @3DPrintingFans2:46 AM – 22 Oct 2015 to 17,194 followers 1 1

The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/uTqZF2VerB

3D Printing @3D_Printing_it2:46 AM – 22 Oct 2015 to 2,209 followers 0 0

The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/m9RZQjmSTP

3D Printing News @3DNewsNow2:46 AM – 22 Oct 2015 to 3,314 followers 0 0
The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/uwk6n5zBsa

3D Printing @3D_Print_News2:46 AM – 22 Oct 2015 to 12,523 followers 0 0

The UPS Store: Personal Service for Small Businesses 3dprint.com/101867/the-ups… #I3DPConf https://t.co/JCTbh1zU5y

Voltivo @voltivo2:04 AM – 22 Oct 2015 to 140 followers 4 0

RT @RealityInc: That’s a lot of 3D printed car parts! #I3DPConf #3Dprinting https://t.co/ctueNEPOG5
Inside3DPrint @Inside3DPrint2:00 AM – 22 Oct 2015 to 3,143 followers 1 4

Thought-provoking & inspirational day 1 #medical track curated by @BunnyEllerin, @NYCHBL! Join us tomorrow for day 2. #I3DPConf #3dprinting

connie874cs@gmail.co @connie874cs1:44 AM – 22 Oct 2015 to 146 followers 4 0

RT @3DPrintGirl: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/ifG…

Rapid Prototyping @rapidprototypin12:55 AM – 22 Oct 2015 to 3,396 followers 1 0

RT @Forecast3D: Brake Bias Knob & Warning Light Panel #3Dprinted in FDM. Come see it IRL @ #I3DPConf in booth 309! #3dprinting #mfg https:/…

Amanda Murray @AmandaFMurray12:46 AM – 22 Oct 2015 to 351 followers 0 1

Hello hills of #California! #i3DPConf here we come! #planeview #weflyhigh #nolie #santaclara @ Santa… instagram.com/p/9HsljxL1wv/

Forecast3D @Forecast3D12:44 AM – 22 Oct 2015 to 172 followers 1 0

Brake Bias Knob & Warning Light Panel #3Dprinted in FDM. Come see it IRL @ #I3DPConf in booth 309! #3dprinting #mfg https://t.co/Fd4vB9bqhw

Intracon @IntraconGlobal12:34 AM – 22 Oct 2015 to 116 followers 2 1

From inoperable to operable: hearing from @Stratasys about the medical applications of #3dprinting. #I3DPConf https://t.co/HlFZ4f9xtY

Mandar @FreeIndian0112:34 AM – 22 Oct 2015 to 8 followers 3 0

RT @Inside3DPrint: Check out @Forecast3D’s #3dprint car w/ drink in hand at the #networking reception until 6pm! #I3DPConf https://t.co/LuX…

spaceclaim @spaceclaim12:29 AM – 22 Oct 2015 to 2,661 followers 1 0

RT @Inside3DPrint: Meet the creator of #perplexus tomorrow at @spaceclaim’s booth! #I3DPConf #3dprinting https://t.co/ECL6tGX4iv

RIVER @RiverVR12:24 AM – 22 Oct 2015 to 194 followers 2 0

RT @IntraconGlobal: Demonstrating the new hardware from @RiverVR, a very minimal viewing device with an emphasis on sharing. #I3DPConf http…

Rapid Prototyping @rapidprototypin12:21 AM – 22 Oct 2015 to 3,396 followers 1 0

RT @Forecast3D: And now… Our #3Dprinted shift knob, done in FDM. PS, it’s cocktail hr here at #I3DPConf! Come on down! #3dprinting https:…

Inside3DPrint @Inside3DPrint12:14 AM – 22 Oct 2015 to 3,143 followers 1 1

Meet the creator of #perplexus tomorrow at @spaceclaim’s booth! #I3DPConf #3dprinting https://t.co/ECL6tGX4iv
Forecast3D @Forecast3D12:13 AM – 22 Oct 2015 to 172 followers 3 0

RT @Inside3DPrint: Check out @Forecast3D’s #3dprint car w/ drink in hand at the #networking reception until 6pm! #I3DPConf https://t.co/LuX…

Forecast3D @Forecast3D12:11 AM – 22 Oct 2015 to 172 followers 1 0

And now… Our #3Dprinted shift knob, done in FDM. PS, it’s cocktail hr here at #I3DPConf! Come on down! #3dprinting https://t.co/wXvPVQiLLA

seancrotty @seancrotty12:11 AM – 22 Oct 2015 to 117 followers 1 1

Good #3Dprinting survey & predictive talk by Tyler Reid of @GoEngineer at #I3DPConf . Slides here: youtube.com/watch?v=3ZUQ4Q…

Inside3DPrint @Inside3DPrint12:10 AM – 22 Oct 2015 to 3,143 followers 3 2

Check out @Forecast3D’s #3dprint car w/ drink in hand at the #networking reception until 6pm! #I3DPConf https://t.co/LuXbUdJThU

Inside3DPrint @Inside3DPrint12:00 AM – 22 Oct 2015 to 3,143 followers 1 0

RT @SJGrunewald: @EnvisionTEC uv cured resin #predator statue. This thing is huge and has amazing detail. #I3DPConf https://t.co/SbdgqiCj4Y

Inside3DPrint @Inside3DPrint12:00 AM – 22 Oct 2015 to 3,143 followers 1 0

RT @Boisevrproject: Excellent discussion on VR in medicine with Albert Rizzo Ph.D of USC #vr #VirtualReality #I3DPConf https://t.co/JqKIm0z…

Bazooka Tech @bazooka_tech11:51 PM – 21 Oct 2015 to 1,173 followers 2 0
RT @EliRibble: Great crowd at #I3DPConf. Thought-provoking questions, exciting businesses, new ideas for applications of #3dprinting

Matthew Rimmer @DrRimmer11:50 PM – 21 Oct 2015 to 8,949 followers 3 0

RT @3DNewsNow: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/FbaTs…

Infinite3D Solutions @Infinite3ds11:44 PM – 21 Oct 2015 to 33 followers 0 1
Ran into the awesome @chucky_larms today at #I3DPConf. Make sure to follow him and check out his… instagram.com/p/9Hlj0znXxG/

Eli Ribble @EliRibble11:40 PM – 21 Oct 2015 to 42 followers 0 1

Printer under-utilization is a business decision not a process or provisioning issue -Karl Willis #I3DPConf

3D Print Week CA @3dprintweekca11:32 PM – 21 Oct 2015 to 81 followers 0 0

6 Ways #3DPrinting Could Transform Your #Thanksgiving Dinner, ft. #I3DPConf Chair Hod Lipson mcklr.me/1W5hxRr (via @PopSci) @Columbia

Inside3DPrint @Inside3DPrint11:32 PM – 21 Oct 2015 to 3,143 followers 0 0

6 Ways #3DPrinting Could Transform Your #Thanksgiving Dinner, ft. #I3DPConf Chair Hod Lipson mcklr.me/1W5hxRr (via @PopSci) @Columbia

Inside3DPrint @Inside3DPrint11:30 PM – 21 Oct 2015 to 3,143 followers 2 0

RT @StewQ: Great preso by @InfraTrac on 3D Fakes: Chemical Security for Additive Manufacturing. #I3DPConf https://t.co/WHmfbHrA6h

Inside3DPrint @Inside3DPrint11:30 PM – 21 Oct 2015 to 3,143 followers 2 0

RT @EliRibble: Great crowd at #I3DPConf. Thought-provoking questions, exciting businesses, new ideas for applications of #3dprinting

Eli Ribble @EliRibble11:30 PM – 21 Oct 2015 to 42 followers 0 1

Single model jobs account for > 1/3 of all prints in sampled bureaus. -Karl Willis #statisticsporn #I3DPConf

Inside3DPrint @Inside3DPrint11:29 PM – 21 Oct 2015 to 3,143 followers 1 0

RT @Boisevrproject: Carolina Cruz-Neria Ph.D discusses VR form factor through the ages. Not a lot of change, so what’s next? #I3DPConf http…

Rapid Prototyping @rapidprototypin11:28 PM – 21 Oct 2015 to 3,396 followers 1 0

RT @Forecast3D: Up next on the #RaceCar: HID Lamp Housing & Seat Air Bulkhead built in FDM. Here @ #I3DPConf! #3dprinting #3dprint https://…

Teacher with #VR @ClementAdert11:28 PM – 21 Oct 2015 to 275 followers 0 1

RT #I3DPConf sessions in #dentistry, #business, #data, #AM, & #VR round out the day … https://t.co/VKTOczruPk https://t.co/qISg1Ctp8v
Authentise @authentise11:25 PM – 21 Oct 2015 to 1,250 followers 1 0

RT @EliRibble: 22% of the time printers are working, 22% complete jobs, 17% space utilization -Karl Willis #I3DPConf

Forecast3D @Forecast3D11:25 PM – 21 Oct 2015 to 172 followers 1 0
RT @aaronilevinson: @Forecast3D used 3D printing to produce nearly 50 parts for this Harbinger! #I3DPConf #3dprinting https://t.co/Lqc6hl8F…

Stewart Quealy @StewQ11:24 PM – 21 Oct 2015 to 8,204 followers 0 1
@KarlDD talking AM Production Data for Analytics. #I3DPConf https://t.co/xDINcmaF9j

Eli Ribble @EliRibble11:24 PM – 21 Oct 2015 to 42 followers 1 1

22% of the time printers are working, 22% complete jobs, 17% space utilization -Karl Willis #I3DPConf

Inside3DPrint @Inside3DPrint11:22 PM – 21 Oct 2015 to 3,143 followers 0 0

Did you know that the #medical and #business tracks are 2 days!? Return tomorrow to learn more about #3Dprinting in those fields! #I3DPConf

Forecast3D @Forecast3D11:20 PM – 21 Oct 2015 to 172 followers 1 1
Up next on the #RaceCar: HID Lamp Housing & Seat Air Bulkhead built in FDM. Here @ #I3DPConf! #3dprinting #3dprint https://t.co/ktejT04giv

Boise VR Project @Boisevrproject11:18 PM – 21 Oct 2015 to 119 followers 1 2

Carolina Cruz-Neria Ph.D discusses VR form factor through the ages. Not a lot of change, so what’s next? #I3DPConf https://t.co/rvVa1Wd0iS

Inside3DPrint @Inside3DPrint11:15 PM – 21 Oct 2015 to 3,143 followers 0 2

#I3DPConf sessions in #dentistry, #business, #data, #AM, & #VR round out the day mcklr.me/1Qy8o2s https://t.co/Ws5h4gPiCe

Peter Westerholm @peter29ner10:59 PM – 21 Oct 2015 to 585 followers 2 0
RT @StewQ: Great preso by @InfraTrac on 3D Fakes: Chemical Security for Additive Manufacturing. #I3DPConf https://t.co/WHmfbHrA6h

Eli Ribble @EliRibble10:57 PM – 21 Oct 2015 to 42 followers 2 3

Great crowd at #I3DPConf. Thought-provoking questions, exciting businesses, new ideas for applications of #3dprinting

Stewart Quealy @StewQ10:55 PM – 21 Oct 2015 to 8,204 followers 2 0
Great preso by @InfraTrac on 3D Fakes: Chemical Security for Additive Manufacturing. #I3DPConf https://t.co/WHmfbHrA6h

Jessica Gurskis @TechGirlSF10:50 PM – 21 Oct 2015 to 99 followers 0 2

The #Cubify band on our world tour…who’s on drums? Inside #3DPrinting conference #I3DPConf… instagram.com/p/9HfYBulVsP/

Identify3D @Identify3D10:44 PM – 21 Oct 2015 to 58 followers 1 0

RT @Inside3DPrint: Think of #additivemanufacturing as a sandwich. #Matrix material is your bread. Sharon Flank, @InfraTrac #I3DPConf https:…

Inside3DPrint @Inside3DPrint10:42 PM – 21 Oct 2015 to 3,143 followers 1 0

Think of #additivemanufacturing as a sandwich. #Matrix material is your bread. Sharon Flank, @InfraTrac #I3DPConf https://t.co/jEeXLYLrU6

Authentise @authentise10:41 PM – 21 Oct 2015 to 1,250 followers 0 1

Thank you everyone!! @EliRibble enjoyed very much. #I3DPConf #manufacturing https://t.co/eRDPC7tLGn
Tyler Benster @tbenst10:40 PM – 21 Oct 2015 to 454 followers 3 0
RT @brianfederal: Be sure to check out Mark Trageser and his amazing creations @ Inside 3D Printing Santa Clara #3Dprinting #I3DPConf

Derek Mathers @MakerMarketer10:39 PM – 21 Oct 2015 to 624 followers 1 0
RT @Inside3DPrint: Now: ‘#3DPrinting 4 Humanity’s Sake’ ft @ElliotKotek @notimpossibl mcklr.me/1hhJpo9 #3dmedical #I3DPConf https://…

Boise VR Project @Boisevrproject10:38 PM – 21 Oct 2015 to 119 followers 1 3

Excellent discussion on VR in medicine with Albert Rizzo Ph.D of USC #vr #VirtualReality #I3DPConf https://t.co/JqKIm0zIVg

TJ Anderson @TJSonOfAnder10:36 PM – 21 Oct 2015 to 2,490 followers 5 0

RT @My3DPrinting: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/Oq…

la moustache ringard @SJGrunewald10:33 PM – 21 Oct 2015 to 234 followers 1 3

@EnvisionTEC uv cured resin #predator statue. This thing is huge and has amazing detail. #I3DPConf https://t.co/SbdgqiCj4Y

Inside3DPrint @Inside3DPrint10:30 PM – 21 Oct 2015 to 3,143 followers 1 3

Now: ‘#3DPrinting 4 Humanity’s Sake’ ft @ElliotKotek @notimpossibl mcklr.me/1hhJpo9 #3dmedical #I3DPConf https://t.co/Z3lmisH5K2

Ray Blackman @Shoeray10:26 PM – 21 Oct 2015 to 517 followers 5 0

RT @My3DPrinting: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/Oq…

ANTO LOPEZ @BIKES410:25 PM – 21 Oct 2015 to 84 followers 4 0

RT @RealityInc: That’s a lot of 3D printed car parts! #I3DPConf #3Dprinting https://t.co/ctueNEPOG5
Inside3DPrint @Inside3DPrint10:21 PM – 21 Oct 2015 to 3,143 followers 4 0

RT @RealityInc: That’s a lot of 3D printed car parts! #I3DPConf #3Dprinting https://t.co/ctueNEPOG5
Inside3DPrint @Inside3DPrint10:21 PM – 21 Oct 2015 to 3,143 followers 3 0

RT @brianfederal: Be sure to check out Mark Trageser and his amazing creations @ Inside 3D Printing Santa Clara #3Dprinting #I3DPConf

Inside3DPrint @Inside3DPrint10:21 PM – 21 Oct 2015 to 3,143 followers 1 0

RT @3DPrint_com: I’m a CAD superhero! Thanks to HoneyPoint3D. #I3DPConf #livefromI3DPConf #HoneyPoint https://t.co/hwbU8TCLpi

Inside3DPrint @Inside3DPrint10:20 PM – 21 Oct 2015 to 3,143 followers 1 0

RT @drbicuspid: We’re covering #digitaldentistry at Inside 3D Printing Conference & Expo. #I3DPConf https://t.co/EITXCI0dC8

Inside3DPrint @Inside3DPrint10:20 PM – 21 Oct 2015 to 3,143 followers 1 0

RT @MatterHackers: MakeIt + MatterControl Touch = match made in #I3DPConf heaven! #MCTouch #MatterControl https://t.co/et70Uh8PfB

WYNIT 3D @WYNIT3D10:19 PM – 21 Oct 2015 to 259 followers 2 0

RT @Inside3DPrint: .@Leapfrog_3D is printing some #halloween pumpkins! #I3DPConf https://t.co/dycEEvlxe2
Ludo.Inc @LudoStreamLine10:19 PM – 21 Oct 2015 to 25 followers 1 0

RT @3DNewsNow: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https://t…

MatterHackers @MatterHackers10:14 PM – 21 Oct 2015 to 830 followers 1 2

MakeIt + MatterControl Touch = match made in #I3DPConf heaven! #MCTouch #MatterControl https://t.co/et70Uh8PfB

Richard Crimmins @RichardCrimmins10:12 PM – 21 Oct 2015 to 112 followers 4 0

RT @Inside3DPrint: Todd Pietila & Jon Detterich: #3dprinted #hearts give sick babies 2nd chances @MaterialiseNV @ChildrensLA #I3DPConf http…

Richard Crimmins @RichardCrimmins10:12 PM – 21 Oct 2015 to 112 followers 4 0

RT @Inside3DPrint: Todd Pietila & Jon Detterich: #3dprinted #hearts give sick babies 2nd chances @MaterialiseNV @ChildrensLA #I3DPConf http…

Inside3DPrint @Inside3DPrint10:11 PM – 21 Oct 2015 to 3,143 followers 0 1

.@bryanjvogel: #additivemanufacturing in the eyes of the #law is evolving #I3DPConf #business https://t.co/72Bn2Uf9bN

DrBicuspid @drbicuspid10:06 PM – 21 Oct 2015 to 14,698 followers 1 3

We’re covering #digitaldentistry at Inside 3D Printing Conference & Expo. #I3DPConf https://t.co/EITXCI0dC8
Tech XB @TechXB10:02 PM – 21 Oct 2015 to 3,394 followers 2 0

RT @Inside3DPrint: #I3DPConf tutorial: integration of #3dprinting in #classrooms mcklr.me/1Xkr21E @L_HoneyPoint3D @HoneyPoint3D http…

3DPrint @3DPrint_com9:57 PM – 21 Oct 2015 to 33,757 followers 1 4

I’m a CAD superhero! Thanks to HoneyPoint3D. #I3DPConf #livefromI3DPConf #HoneyPoint https://t.co/hwbU8TCLpi

Rapid Prototyping @rapidprototypin9:57 PM – 21 Oct 2015 to 3,396 followers 2 0

RT @RealityInc: New York Times is shipping out free Google Cardboard to readers. Get on top of VR #3DPrinting people! #I3DPConf

MIAOmiao Zhao @MIAOmiaoZhao19:56 PM – 21 Oct 2015 to 117 followers 2 0

RT @Inside3DPrint: .@Leapfrog_3D is printing some #halloween pumpkins! #I3DPConf https://t.co/dycEEvlxe2
Inside3DPrint @Inside3DPrint9:54 PM – 21 Oct 2015 to 3,143 followers 4 4

Todd Pietila & Jon Detterich: #3dprinted #hearts give sick babies 2nd chances @MaterialiseNV @ChildrensLA #I3DPConf https://t.co/tTfaug8buX

Danielle Matich @DanielleMatich9:51 PM – 21 Oct 2015 to 86 followers 2 0

RT @RealityInc: New York Times is shipping out free Google Cardboard to readers. Get on top of VR #3DPrinting people! #I3DPConf

Inside3DPrint @Inside3DPrint9:50 PM – 21 Oct 2015 to 3,143 followers 2 4

.@Leapfrog_3D is printing some #halloween pumpkins! #I3DPConf https://t.co/dycEEvlxe2

Brian Federal @brianfederal9:48 PM – 21 Oct 2015 to 10,290 followers 4 0

RT @lulzbot3D: If you’re at #I3DPConf in Santa Clara, stop by booth 505! We’re hanging out with @Proto_pasta, too! @Inside3DPrint #3dprinti…

Brian Federal @brianfederal9:48 PM – 21 Oct 2015 to 10,290 followers 1 0

RT @StewQ: Stellar opening keynote with Andrew Rush of @MadeInSpace #I3DPConf https://t.co/o4oOKUmXB4
Brian Federal @brianfederal9:48 PM – 21 Oct 2015 to 10,290 followers 1 0

RT @Inside3DPrint: .@StewQ asks #I3DPConf 1st timers about what brought them here. If you’re in Santa Clara, what led you to attend? https:…
Brian Federal @brianfederal9:47 PM – 21 Oct 2015 to 10,290 followers 3 0

RT @formlabs: Day 2 of #I3DPConf Santa Clara! Interested in stereolithography #3dprinting technology? Stop by booth 502 to see the Form 2 a…
Brian Federal @brianfederal9:47 PM – 21 Oct 2015 to 10,290 followers 5 0

RT @PreetJesrani: Many thanks to @3dprintindustry @RealityInc for stopping by to visit @ZMorph3d @Inside3DPrint #I3DPConf #3DPrinting https…

Brian Federal @brianfederal9:47 PM – 21 Oct 2015 to 10,290 followers 1 0

RT @3dprintweekca: Today at #I3DPConf: @MadeInSpace Keynote Examines #AM Among the Stars mcklr.me/1MEN6MX (via @3DPrint_com) #3dprin…

Reality™ Institute @RealityInc9:43 PM – 21 Oct 2015 to 534 followers 2 2

New York Times is shipping out free Google Cardboard to readers. Get on top of VR #3DPrinting people! #I3DPConf

Liza Wallach Kloski @L_HoneyPoint3D9:43 PM – 21 Oct 2015 to 274 followers 2 0

RT @Inside3DPrint: #I3DPConf tutorial: integration of #3dprinting in #classrooms mcklr.me/1Xkr21E @L_HoneyPoint3D @HoneyPoint3D http…

Rapid Prototyping @rapidprototypin9:39 PM – 21 Oct 2015 to 3,396 followers 4 0

RT @RealityInc: That’s a lot of 3D printed car parts! #I3DPConf #3Dprinting https://t.co/ctueNEPOG5
alex@fabbing @fabbing9:37 PM – 21 Oct 2015 to 8,565 followers 3 0

RT @brianfederal: Be sure to check out Mark Trageser and his amazing creations @ Inside 3D Printing Santa Clara #3Dprinting #I3DPConf

3D Forged @3DForged9:32 PM – 21 Oct 2015 to 3,528 followers 4 0
RT @lulzbot3D: If you’re at #I3DPConf in Santa Clara, stop by booth 505! We’re hanging out with @Proto_pasta, too! @Inside3DPrint #3dprinti…

3D Forged @3DForged9:30 PM – 21 Oct 2015 to 3,528 followers 2 0
RT @Inside3DPrint: Sweet spot for #additivemanufacturing: quantities in lower volume, higher complexity, & opportunity to redesign – Rich G…
Reality™ Institute @RealityInc9:25 PM – 21 Oct 2015 to 534 followers 4 5

That’s a lot of 3D printed car parts! #I3DPConf #3Dprinting https://t.co/ctueNEPOG5

Reality™ Institute @RealityInc9:24 PM – 21 Oct 2015 to 534 followers 5 0

RT @PreetJesrani: Many thanks to @3dprintindustry @RealityInc for stopping by to visit @ZMorph3d @Inside3DPrint #I3DPConf #3DPrinting https…

Tracy Spahr @fnnygrl739:23 PM – 21 Oct 2015 to 670 followers 3 0

RT @Inside3DPrint: Submit your ?’s for @TheUPSStore panel by 10/22 using #3DPWeek mcklr.me/1LmSvbw #I3DPConf #3dprinting @3d_printer…

Brian Federal @brianfederal9:21 PM – 21 Oct 2015 to 10,290 followers 3 1

Be sure to check out Mark Trageser and his amazing creations @ Inside 3D Printing Santa Clara #3Dprinting #I3DPConf

Kurt @KommonKurtesy9:19 PM – 21 Oct 2015 to 123 followers 2 0

RT @Inside3DPrint: Sweet spot for #additivemanufacturing: quantities in lower volume, higher complexity, & opportunity to redesign – Rich G…
Inside3DPrint @Inside3DPrint9:13 PM – 21 Oct 2015 to 3,143 followers 2 2

Sweet spot for #additivemanufacturing: quantities in lower volume, higher complexity, & opportunity to redesign – Rich Garrity #I3DPConf

Mohammed Ali @jayce_ali9:05 PM – 21 Oct 2015 to 305 followers 4 0

RT @3DPrintGirl: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/ifG…

John Hornick @JHornick3D1Stop9:03 PM – 21 Oct 2015 to 313 followers 1 0

RT @Inside3DPrint: 11:45 #business session: @JHornick3D1Stop, @FinneganIPLaw on #3dprinting & future/demise of #IP mcklr.me/1VTiWQm …

Bezzinga @stevejh749:02 PM – 21 Oct 2015 to 320 followers 3 0
RT @3DNewsNow: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/FbaTs…

3D Print Week CA @3dprintweekca8:59 PM – 21 Oct 2015 to 81 followers 1 1

Today at #I3DPConf: @MadeInSpace Keynote Examines #AM Among the Stars mcklr.me/1MEN6MX (via @3DPrint_com) #3dprinting @RushSpace

MecklerMedia @MecklerMedia8:59 PM – 21 Oct 2015 to 305 followers 0 1

Today at #I3DPConf: @MadeInSpace Keynote Examines #AM Among the Stars mcklr.me/1MEN6MX (via @3DPrint_com) #3dprinting @RushSpace

Inside3DPrint @Inside3DPrint8:59 PM – 21 Oct 2015 to 3,143 followers 0 1

Today at #I3DPConf: @MadeInSpace Keynote Examines #AM Among the Stars mcklr.me/1MEN6MX (via @3DPrint_com) #3dprinting @RushSpace

J. Nayer Hardin @cureworks8:58 PM – 21 Oct 2015 to 4,587 followers 1 0
RT @3DPrint_com: Speaking now: Rich Garrity, Stratasys afternoon keynote, applications for AM #I3DPConf #livefromI3DPConf https://t.co/vyYy…

Brandan Mueller @BrandanMueller8:58 PM – 21 Oct 2015 to 33 followers 2 0

RT @Inside3DPrint: Crowded room for @HuschBlackwell’s sponsored session at #I3DPConf Santa Clara #3dprinting https://t.co/CaCU7kYBWy

Startuphangar.com @startuphangar8:57 PM – 21 Oct 2015 to 2,926 followers 5 0

RT @My3DPrinting: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/Oq…

3DPrint @3DPrint_com8:57 PM – 21 Oct 2015 to 33,757 followers 1 1

Speaking now: Rich Garrity, Stratasys afternoon keynote, applications for AM #I3DPConf #livefromI3DPConf https://t.co/vyYyDarx8Q

DB3D Distribution @DB3Distribution8:55 PM – 21 Oct 2015 to 408 followers 5 0

RT @PreetJesrani: Many thanks to @3dprintindustry @RealityInc for stopping by to visit @ZMorph3d @Inside3DPrint #I3DPConf #3DPrinting https…

DesignBox3D @DesignBox3D8:55 PM – 21 Oct 2015 to 2,994 followers 5 0

RT @PreetJesrani: Many thanks to @3dprintindustry @RealityInc for stopping by to visit @ZMorph3d @Inside3DPrint #I3DPConf #3DPrinting https…

Preet Jesrani @PreetJesrani8:55 PM – 21 Oct 2015 to 196 followers 5 12

Many thanks to @3dprintindustry @RealityInc for stopping by to visit @ZMorph3d @Inside3DPrint #I3DPConf #3DPrinting https://t.co/vb35MIRZ3W

Aaron Levinson @aaronilevinson8:52 PM – 21 Oct 2015 to 57 followers 0 3

OEMs need to reduce part weight & reduce wasted raw materials used for production. What better way than #3dprinting? #I3DPConf

Rapid Prototyping @rapidprototypin8:50 PM – 21 Oct 2015 to 3,396 followers 3 0

RT @3DNewsNow: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/FbaTs…

BK @__BK3__8:50 PM – 21 Oct 2015 to 1,151 followers 4 0
RT @Inside3DPrint: Keynote from Rich Garrity, @Stratasys on the future of #additivemanufacturing is starting now! #I3DPConf #3dprinting htt…

alex@fabbing @fabbing8:50 PM – 21 Oct 2015 to 8,565 followers 3 0

RT @3DPrintBoard: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/RZ…

Kurt @KommonKurtesy8:49 PM – 21 Oct 2015 to 123 followers 4 0

RT @Inside3DPrint: Keynote from Rich Garrity, @Stratasys on the future of #additivemanufacturing is starting now! #I3DPConf #3dprinting htt…

Inside3DPrint @Inside3DPrint8:48 PM – 21 Oct 2015 to 3,143 followers 2 0

RT @Stratasys: MT @Inside3DPrint: Keynote from Rich Garrity on future of #additivemanufacturing starts now! #I3DPConf #3dprinting https://t…

Dina @dina08068:48 PM – 21 Oct 2015 to 46 followers 2 0
RT @Inside3DPrint: The power of #additivemanufacturing starts in the #design process, says Rich Garrity, @Stratasys #I3DPConf #3dprinting

Infinite3D Solutions @Infinite3ds8:47 PM – 21 Oct 2015 to 33 followers 2 0
RT @Inside3DPrint: The power of #additivemanufacturing starts in the #design process, says Rich Garrity, @Stratasys #I3DPConf #3dprinting

Infinite3D Solutions @Infinite3ds8:47 PM – 21 Oct 2015 to 33 followers 5 0
RT @My3DPrinting: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/Oq…

Infinite3D Solutions @Infinite3ds8:47 PM – 21 Oct 2015 to 33 followers 3 0
RT @3DPrintBoard: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/RZ…

Inside3DPrint @Inside3DPrint8:47 PM – 21 Oct 2015 to 3,143 followers 1 0

RT @Infinite3ds: Thank you @Inside3DPrint and @MecklerMedia for today’s fun at #I3DPConf! #3dprint

Stratasys @3d_printers8:47 PM – 21 Oct 2015 to 50,066 followers 4 0
RT @Inside3DPrint: Keynote from Rich Garrity, @Stratasys on the future of #additivemanufacturing is starting now! #I3DPConf #3dprinting htt…

Inside3DPrint @Inside3DPrint8:46 PM – 21 Oct 2015 to 3,143 followers 2 2

The power of #additivemanufacturing starts in the #design process, says Rich Garrity, @Stratasys #I3DPConf #3dprinting

Infinite3D Solutions @Infinite3ds8:45 PM – 21 Oct 2015 to 33 followers 1 1
Thank you @Inside3DPrint and @MecklerMedia for today’s fun at #I3DPConf! #3dprint

3D Printing News @My3DPrinting8:45 PM – 21 Oct 2015 to 54,848 followers 5 2

Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/OqFEleol66

3D Print .com @3DPrintBoard8:45 PM – 21 Oct 2015 to 46,499 followers 3 0

Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/RZd0b8IhYh

3D Printing @3D_Printing_it8:45 PM – 21 Oct 2015 to 2,209 followers 0 0

Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/9k3WpILmEr

3D Printing News @3DNewsNow8:45 PM – 21 Oct 2015 to 3,314 followers 3 1
Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/FbaTsIUB3Y

3DPrint @3DPrint_com8:45 PM – 21 Oct 2015 to 33,757 followers 2 3

Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/nxWkNnehuW

Startuphangar.com @startuphangar8:44 PM – 21 Oct 2015 to 2,926 followers 2 0

RT @3DPrintingFans: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/…

Startuphangar.com @startuphangar8:44 PM – 21 Oct 2015 to 2,926 followers 3 0

RT @3DPrintingIt: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/sz…

Inside3DPrint @Inside3DPrint8:43 PM – 21 Oct 2015 to 3,143 followers 1 0

RT @SJGrunewald: “Everything will change when we can make anything” -great talk from John Hornick at #I3DPConf ip won’t go away but it will…

Inside3DPrint @Inside3DPrint8:43 PM – 21 Oct 2015 to 3,143 followers 1 0

RT @aaronilevinson: Side mirrors, wheel caps, headlamp housings, air flow diverters, ducts & vents 3D printed by @Forecast3D #I3DPConf http…

Stratasys @Stratasys8:43 PM – 21 Oct 2015 to 32,354 followers 2 4
MT @Inside3DPrint: Keynote from Rich Garrity on future of #additivemanufacturing starts now! #I3DPConf #3dprinting https://t.co/C8zjwLmnPx

Infinite3D Solutions @Infinite3ds8:41 PM – 21 Oct 2015 to 33 followers 0 1
We made it! Inside 3D Printing Conference & Expo! #I3DPConf #Infinite3Dsolutions #I3DS #3Dselfie… instagram.com/p/9HQmaDnXxh/

Identify3D @Identify3D8:40 PM – 21 Oct 2015 to 58 followers 1 0

RT @Inside3DPrint: Richard Garrity, @Stratasys KEYNOTE ‘Latest Advancements in Industrial Apps for #AdditiveManufacturing’ https://t.co/r3I…

Rapid Prototyping @rapidprototypin8:38 PM – 21 Oct 2015 to 3,396 followers 1 0

RT @3DPrintBoardcom: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co…

Inside3DPrint @Inside3DPrint8:37 PM – 21 Oct 2015 to 3,143 followers 4 4

Keynote from Rich Garrity, @Stratasys on the future of #additivemanufacturing is starting now! #I3DPConf #3dprinting https://t.co/daPvsvKKyQ

Bazooka Tech @bazooka_tech8:36 PM – 21 Oct 2015 to 1,173 followers 4 0
RT @3DForged: At the UPS Store Booth @ #I3DPConf. When UPS jumps into a new technology, you know it’s a big deal. #3dprinting https://t.co/…

Peter Westerholm @peter29ner8:35 PM – 21 Oct 2015 to 585 followers 4 0
RT @3DForged: At the UPS Store Booth @ #I3DPConf. When UPS jumps into a new technology, you know it’s a big deal. #3dprinting https://t.co/…

Inside3DPrint @Inside3DPrint8:30 PM – 21 Oct 2015 to 3,143 followers 1 0

Richard Garrity, @Stratasys KEYNOTE ‘Latest Advancements in Industrial Apps for #AdditiveManufacturing’ mcklr.me/1NFtBIk #I3DPConf

カモノハシ @roso19548:25 PM – 21 Oct 2015 to 9,915 followers 0 0
＠3DPrintGirl: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPr… https://t.co/SKCunS7a45

steven Altman @stevenAltman28:18 PM – 21 Oct 2015 to 948 followers 2 0

RT @seancrotty: Arrived at the Inside 3D Printing Conference in Santa Clara #I3DPConf for innovation & IP strategy opps with https://t.co/X…
Bazooka Tech @bazooka_tech8:17 PM – 21 Oct 2015 to 1,173 followers 3 0
RT @3DPrintingIt: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/sz…

BayAreaStartupEvents @BayAreaStartup8:17 PM – 21 Oct 2015 to 609 followers 2 0

RT @seancrotty: Arrived at the Inside 3D Printing Conference in Santa Clara #I3DPConf for innovation & IP strategy opps with https://t.co/X…
nikita w @031nikitaW8:15 PM – 21 Oct 2015 to 1,922 followers 3 0

RT @3DPrintingIt: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/sz…

3D Printer News @3DPrinterNews8:14 PM – 21 Oct 2015 to 1,078 followers 0 0

twitter.com/3DPrintBoardco… Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars … https://t.co/xwvUMMbOfQ
Three Blind Mice @ThreeBlindMice68:14 PM – 21 Oct 2015 to 9,507 followers 2 0

RT @3DPrintingFans: Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/…

3D Printing Fans @3DPrintingFans8:12 PM – 21 Oct 2015 to 17,194 followers 2 2

Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/bAQa5VlkLD

3D Printing @3D_Print_News8:12 PM – 21 Oct 2015 to 12,523 followers 0 0

Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/j0zqJmS76b

3D Printing @3DPrintingIt8:12 PM – 21 Oct 2015 to 15,317 followers 3 1

Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/szkD1FY4fV

3D Printing @3DPrintBoardcom8:12 PM – 21 Oct 2015 to 11,478 followers 1 0

Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/aKbsGpU9Re

3D Printing News @3DPrintGirl8:12 PM – 21 Oct 2015 to 40,617 followers 4 3

Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars 3dprint.com/101804/made-in… #3DPrinting https://t.co/ifG9uY8pid

3DMetalPrintMag @3DMetalPrintMag8:08 PM – 21 Oct 2015 to 40 followers 1 0

RT @Inside3DPrint: Booth 221: Find out about @PMATalk & @MecklerMedia’s new #3DMetalPrinting Mag mcklr.me/1hJjcyw #I3DPConf https://…

Infinite3D Solutions @Infinite3ds8:06 PM – 21 Oct 2015 to 33 followers 0 0
Awesome booths at the Inside 3D Print Expo! #I3DPConf

Cloud4Logistics @warehouseit8:05 PM – 21 Oct 2015 to 4,045 followers 3 0

RT @3DForged: Print, scan, fax? The @AIORobotics Zeus does it all. They might be the coolest guys at the #I3DPConf too. https://t.co/Kbou5w…

Aaron Levinson @aaronilevinson8:03 PM – 21 Oct 2015 to 57 followers 1 1

This MASSIVE Perplexus puzzle was developed with @spaceclaim software. I need one for my house! #I3DPConf https://t.co/RqURm5N7De

Inside3DPrint @Inside3DPrint8:00 PM – 21 Oct 2015 to 3,143 followers 1 0

Booth 221: Find out about @PMATalk & @MecklerMedia’s new #3DMetalPrinting Mag mcklr.me/1hJjcyw #I3DPConf https://t.co/FXddI7XPdT

Brent Hale @BrentAHale7:58 PM – 21 Oct 2015 to 12,682 followers 4 0

RT @3DForged: At the UPS Store Booth @ #I3DPConf. When UPS jumps into a new technology, you know it’s a big deal. #3dprinting https://t.co/…

Brent Hale @BrentAHale7:58 PM – 21 Oct 2015 to 12,682 followers 3 0

RT @3DForged: Print, scan, fax? The @AIORobotics Zeus does it all. They might be the coolest guys at the #I3DPConf too. https://t.co/Kbou5w…

HomeZilla @homezillaorguk7:58 PM – 21 Oct 2015 to 821 followers 3 0

RT @3DForged: Print, scan, fax? The @AIORobotics Zeus does it all. They might be the coolest guys at the #I3DPConf too. https://t.co/Kbou5w…

3D Forged @3DForged7:58 PM – 21 Oct 2015 to 3,528 followers 3 4
Print, scan, fax? The @AIORobotics Zeus does it all. They might be the coolest guys at the #I3DPConf too. https://t.co/Kbou5wChFj

Aaron Levinson @aaronilevinson7:57 PM – 21 Oct 2015 to 57 followers 0 1

…but only AFTER I’ve had some coffee…#I3DPConf https://t.co/HKpxsx9gpp

Boise VR Project @Boisevrproject7:56 PM – 21 Oct 2015 to 119 followers 2 0

RT @IntraconGlobal: Demonstrating the new hardware from @RiverVR, a very minimal viewing device with an emphasis on sharing. #I3DPConf http…

3D Forged @3DForged7:55 PM – 21 Oct 2015 to 3,528 followers 4 5
At the UPS Store Booth @ #I3DPConf. When UPS jumps into a new technology, you know it’s a big deal. #3dprinting https://t.co/obwCTIso3q

Quickparts Solutions @Quickparts7:54 PM – 21 Oct 2015 to 1,590 followers 9 0
RT @tbenst: Beautiful titanium print by @3dsystemscorp #I3DPConf https://t.co/Ie21Cv0tls

Aaron Levinson @aaronilevinson7:54 PM – 21 Oct 2015 to 57 followers 0 1

This 3D printed dress was produced on a home printer. Wow! It’s flexible & light. @lulzbot3D #I3DPConf #3dprinting https://t.co/0Xu0IhoFA6

Aaron Levinson @aaronilevinson7:51 PM – 21 Oct 2015 to 57 followers 0 0

This chess set is only 2″x2″! I want one! @HoganasAB #I3DPConf https://t.co/3Qs5NCOKCu
Aaron Levinson @aaronilevinson7:48 PM – 21 Oct 2015 to 57 followers 1 1

Side mirrors, wheel caps, headlamp housings, air flow diverters, ducts & vents 3D printed by @Forecast3D #I3DPConf https://t.co/1Z20XjiXV0

Inside3DPrint @Inside3DPrint7:45 PM – 21 Oct 2015 to 3,143 followers 2 2

#I3DPConf tutorial: integration of #3dprinting in #classrooms mcklr.me/1Xkr21E @L_HoneyPoint3D @HoneyPoint3D https://t.co/Nt09gB5aUx

Aaron Levinson @aaronilevinson7:44 PM – 21 Oct 2015 to 57 followers 1 2

Spolier, tail light lenses, quarter panel ext…all 3D printed by @Forecast3D! #I3DPConf https://t.co/EPwnuLxixI
la moustache ringard @SJGrunewald7:43 PM – 21 Oct 2015 to 234 followers 1 0

“Everything will change when we can make anything” -great talk from John Hornick at #I3DPConf ip won’t go away but it will be much different

Aaron Levinson @aaronilevinson7:42 PM – 21 Oct 2015 to 57 followers 1 2

@Forecast3D used 3D printing to produce nearly 50 parts for this Harbinger! #I3DPConf #3dprinting https://t.co/Lqc6hl8F41

Aaron Levinson @aaronilevinson7:35 PM – 21 Oct 2015 to 57 followers 0 0

Size DOES matter! Check out @Raise3D ‘s N2 printer with 12″x12″x24″ build capacity. #I3DPConf #3dprinting https://t.co/QvPo7D6MuW

Intracon @IntraconGlobal7:33 PM – 21 Oct 2015 to 116 followers 2 3

Demonstrating the new hardware from @RiverVR, a very minimal viewing device with an emphasis on sharing. #I3DPConf https://t.co/NJbcNChxZg

Rapid Prototyping @rapidprototypin7:33 PM – 21 Oct 2015 to 3,396 followers 1 0

RT @Forecast3D: #3DPrint Part 1&2 on the #RaceCar: Airbox & Mounting Brackets, done in FDM. PS, come see us @ #I3DPConf #3dprinting https:/…

Inside3DPrint @Inside3DPrint7:30 PM – 21 Oct 2015 to 3,143 followers 1 1

Stay in the conversation! Use ‘#I3DPConf’ to share your thoughts on the Santa Clara show https://t.co/JtpAlXvHLx
Rodrigo Bossle @Rodrigo_Bossle7:24 PM – 21 Oct 2015 to 684 followers 10 0

RT @SJGrunewald: @GoEngineer 3D printed #halo figure. One piece on #objetconnex @Stratasys multi color and materials. #I3DPConf https://t.c…

Worrell Design @WorrellDesign7:19 PM – 21 Oct 2015 to 851 followers 0 0

@medtronic’s Srinivasan Varahoor discussing what is needed to print models for their key doctors #I3DPconf https://t.co/PJ2nyf47Q2

Yoni Binstock @YoniBinstock7:16 PM – 21 Oct 2015 to 462 followers 0 0

At #i3dpconf? Then you should check out the top eBook on #3DPrinting #Entrepreneurship – bit.ly/3DEntrepreneur

Forecast3D @Forecast3D7:13 PM – 21 Oct 2015 to 172 followers 1 0
#3DPrint Part 1&2 on the #RaceCar: Airbox & Mounting Brackets, done in FDM. PS, come see us @ #I3DPConf #3dprinting https://t.co/smnuXdO8ic

Scansite @Scansite3D7:08 PM – 21 Oct 2015 to 13 followers 1 0
RT @Inside3DPrint: “#Resolution & #samplingdensity- the most misunderstood terms in #3dprinting” @Scansite3D #I3DPConf https://t.co/WN9eOVg…

Inside3DPrint @Inside3DPrint7:01 PM – 21 Oct 2015 to 3,143 followers 1 0

“#Resolution & #samplingdensity- the most misunderstood terms in #3dprinting” @Scansite3D #I3DPConf https://t.co/WN9eOVgIVT

Boise VR Project @Boisevrproject6:51 PM – 21 Oct 2015 to 119 followers 1 0

RT @IntraconGlobal: What are #I3DPConf experts seeing for #VR in education? Industry overall isn’t investing much yet but it’s already a bi…
Geo @GeoDelGonzo6:51 PM – 21 Oct 2015 to 153 followers 2 0

RT @3DForged: Printed on the @Zortrax_3D M200… #I3DPConf #3dprinting https://t.co/SbcW993t5o
Edmond Dantes @Edg_Art_ValFlo6:50 PM – 21 Oct 2015 to 69 followers 0 0

#I3DPConf eSun 3D filament !

la moustache ringard @SJGrunewald6:48 PM – 21 Oct 2015 to 234 followers 2 0

RT @3DPrint_com: “Recycling is key,” says Made in Space president, for “Earth-independent production.” #I3DPConf #livefromI3DPConf

la moustache ringard @SJGrunewald6:48 PM – 21 Oct 2015 to 234 followers 0 1

The shine on my forehead is a feature, not a flaw, I swear. #I3DPConf twitter.com/3dprint_com/st…

3D Print Week CA @3dprintweekca6:47 PM – 21 Oct 2015 to 81 followers 2 0

RT @3DPrint_com: On to the Exhibit Hall! Say hi if you see us! Editor-in-Chief Sarah, writer Scott at #I3DPConf #livefromI3DPConf https://t…

3D Print Week CA @3dprintweekca6:47 PM – 21 Oct 2015 to 81 followers 1 0

RT @Inside3DPrint: The exhibit hall is buzzing at #I3DPCONF Santa Clara! https://t.co/K7RhPEV0SB
Inside3DPrint @Inside3DPrint6:45 PM – 21 Oct 2015 to 3,143 followers 3 0

RT @formlabs: Day 2 of #I3DPConf Santa Clara! Interested in stereolithography #3dprinting technology? Stop by booth 502 to see the Form 2 a…
la moustache ringard @SJGrunewald6:44 PM – 21 Oct 2015 to 234 followers 0 0

How will #3dprinting impact ip law? Let’s find out. 3dp & the future (or demise) of ip @ business tract w/ John Hornick #I3DPConf

alex@fabbing @fabbing6:30 PM – 21 Oct 2015 to 8,565 followers 3 0

RT @formlabs: Day 2 of #I3DPConf Santa Clara! Interested in stereolithography #3dprinting technology? Stop by booth 502 to see the Form 2 a…
Inside3DPrint @Inside3DPrint6:30 PM – 21 Oct 2015 to 3,143 followers 1 0

11:45 #business session: @JHornick3D1Stop, @FinneganIPLaw on #3dprinting & future/demise of #IP mcklr.me/1VTiWQm #I3DPConf

Inside3DPrint @Inside3DPrint6:26 PM – 21 Oct 2015 to 3,143 followers 1 1

“In 10 years, we will not take a #vacation without being there virtually first” @SivanIram @RiverVR @rothventures #virtualreality #I3DPConf

Brent Hale @BrentAHale6:25 PM – 21 Oct 2015 to 12,682 followers 1 0

RT @3DForged: Octave booth #I3DPConf #3dprinting https://t.co/R9bsH9J3yG

Brent Hale @BrentAHale6:25 PM – 21 Oct 2015 to 12,682 followers 1 0

RT @3DForged: Tons of cool filament from Polymaker… wood filament, flexi filament and a ton more. #I3DPConf #3dprinting https://t.co/cZPY…

Brent Hale @BrentAHale6:25 PM – 21 Oct 2015 to 12,682 followers 2 0

RT @3DForged: Printed on the @Zortrax_3D M200… #I3DPConf #3dprinting https://t.co/SbcW993t5o
3D Printing @Just_Print_It6:24 PM – 21 Oct 2015 to 839 followers 10 0

RT @SJGrunewald: @GoEngineer 3D printed #halo figure. One piece on #objetconnex @Stratasys multi color and materials. #I3DPConf https://t.c…

3D Forged @3DForged6:23 PM – 21 Oct 2015 to 3,528 followers 2 1
Printed on the @Zortrax_3D M200… #I3DPConf #3dprinting https://t.co/SbcW993t5o

Bazooka Tech @bazooka_tech6:21 PM – 21 Oct 2015 to 1,173 followers 3 0
RT @Inside3DPrint: Submit your ?’s for @TheUPSStore panel by 10/22 using #3DPWeek mcklr.me/1LmSvbw #I3DPConf #3dprinting @3d_printer…

Terry Wohlers @TerryWohlers6:20 PM – 21 Oct 2015 to 3,756 followers 3 0

RT @Inside3DPrint: Submit your ?’s for @TheUPSStore panel by 10/22 using #3DPWeek mcklr.me/1LmSvbw #I3DPConf #3dprinting @3d_printer…

la moustache ringard @SJGrunewald6:18 PM – 21 Oct 2015 to 234 followers 0 0

#chimak3d Pinin Avail next month. Kit $500 assemble under 1k. Pastabot $350 lets opensource 3dp w/ceramic #I3DPConf https://t.co/2kXbVxfRtS

iViewing @iViewing6:17 PM – 21 Oct 2015 to 467 followers 1 0

RT @sffashiontech: The frontier of innovation is seen via millenial founders, esp. as it relates to #VR #AR. @sivaniram @riverVR @rothventu…

3D Forged @3DForged6:16 PM – 21 Oct 2015 to 3,528 followers 1 1
Tons of cool filament from Polymaker… wood filament, flexi filament and a ton more. #I3DPConf #3dprinting https://t.co/cZPYsyH6cv

Stewart Quealy @StewQ6:15 PM – 21 Oct 2015 to 8,204 followers 0 0
Afternoon keynote with @SivanIram of @RiverVR and @SandraKayHelsel #I3DPConf https://t.co/JUVuC6HAan
Dina @dina08066:13 PM – 21 Oct 2015 to 46 followers 10 0
RT @SJGrunewald: @GoEngineer 3D printed #halo figure. One piece on #objetconnex @Stratasys multi color and materials. #I3DPConf https://t.c…

Geo @GeoDelGonzo6:13 PM – 21 Oct 2015 to 153 followers 10 0

RT @SJGrunewald: @GoEngineer 3D printed #halo figure. One piece on #objetconnex @Stratasys multi color and materials. #I3DPConf https://t.c…

Rapid Prototyping @rapidprototypin6:13 PM – 21 Oct 2015 to 3,396 followers 2 0

RT @Forecast3D: What is this #RaceCar we keep talkin about? Stay tuned, we’ll be tweeting pics of each #3DP part during #I3DPConf! #3dprint…

3D Forged @3DForged6:12 PM – 21 Oct 2015 to 3,528 followers 1 1
Octave booth #I3DPConf #3dprinting https://t.co/R9bsH9J3yG

SF Fashtech @sffashiontech6:11 PM – 21 Oct 2015 to 2,679 followers 1 0

The frontier of innovation is seen via millenial founders, esp. as it relates to #VR #AR. @sivaniram @riverVR @rothventures #I3DPConf

Teacher with #VR @ClementAdert6:07 PM – 21 Oct 2015 to 275 followers 0 0

RT RT GreenfishLabs: If u are at the #I3DPConf 2day, check out VR in Mktg and Branding session 2 hear our team talk… https://t.co/m4u815YJuk
Terry Wohlers @TerryWohlers6:06 PM – 21 Oct 2015 to 3,756 followers 1 0

RT @TheUPSStore: Remember to submit your questions for @TerryWohlers & @3d_printers of experts at #I3DPConf using #3DPWeek! https://t.co/Iq…
Sovanna Mam @SovannaMam6:05 PM – 21 Oct 2015 to 385 followers 4 0

RT @GreenfishLabs: If u are at the #I3DPConf 2day, check out VR in Mktg and Branding session 2 hear our team talk about #VR in advertising!…

Inside3DPrint @Inside3DPrint6:02 PM – 21 Oct 2015 to 3,143 followers 1 1

The exhibit hall is buzzing at #I3DPCONF Santa Clara! https://t.co/K7RhPEV0SB

Formlabs @formlabs6:01 PM – 21 Oct 2015 to 9,648 followers 3 4

Day 2 of #I3DPConf Santa Clara! Interested in stereolithography #3dprinting technology? Stop by booth 502 to see the Form 2 and learn more
FlowchartPersonality @djgeki5:57 PM – 21 Oct 2015 to 395 followers 10 0
RT @SJGrunewald: @GoEngineer 3D printed #halo figure. One piece on #objetconnex @Stratasys multi color and materials. #I3DPConf https://t.c…

By The Flamingos @ByTheFlamingos5:57 PM – 21 Oct 2015 to 99 followers 2 0

RT @SJGrunewald: @MadeInSpace Working on recycling materials, working w/ conductives, metal materials & exploring #3dprinting moon base. #I…

Teacher with #VR @ClementAdert5:53 PM – 21 Oct 2015 to 275 followers 0 0

RT What are #I3DPConf experts seeing for #VR in education? Industry overall isn’t investing much yet but it’s alrea… https://t.co/DUYT3cmbc4
3DPrint @3DPrint_com5:51 PM – 21 Oct 2015 to 33,757 followers 2 0

RT @SJGrunewald: @MadeInSpace Working on recycling materials, working w/ conductives, metal materials & exploring #3dprinting moon base. #I…

3DPrint @3DPrint_com5:51 PM – 21 Oct 2015 to 33,757 followers 1 0

RT @SJGrunewald: #raise3d on #Kickstarter can print 85 degree overhang. 10 materials. Extruded temp 300 degrees. #I3DPConf https://t.co/zs…

3DPrint @3DPrint_com5:51 PM – 21 Oct 2015 to 33,757 followers 1 0

RT @SJGrunewald: @ff3dprinters new #flashforge #finder low cost printer available next month. Projected price $699. #I3DPConf https://t.co/…

3DPrint @3DPrint_com5:51 PM – 21 Oct 2015 to 33,757 followers 10 0

RT @SJGrunewald: @GoEngineer 3D printed #halo figure. One piece on #objetconnex @Stratasys multi color and materials. #I3DPConf https://t.c…

GoEngineer @GoEngineer5:49 PM – 21 Oct 2015 to 1,689 followers 0 0

What caught your eye at our booth? #GoEngineer #Booth315 #i3DPConf https://t.co/GVArM1lAl5
Intracon @IntraconGlobal5:49 PM – 21 Oct 2015 to 116 followers 1 1

What are #I3DPConf experts seeing for #VR in education? Industry overall isn’t investing much yet but it’s already a big factor for parents.
Brent Hale @BrentAHale5:47 PM – 21 Oct 2015 to 12,682 followers 1 0

RT @3DForged: Finally here at the Inside 3D Printing Conference! #I3DPConf https://t.co/UpPP6bmrp2
3D Forged @3DForged5:47 PM – 21 Oct 2015 to 3,528 followers 1 1
Finally here at the Inside 3D Printing Conference! #I3DPConf https://t.co/UpPP6bmrp2

Inside3DPrint @Inside3DPrint5:46 PM – 21 Oct 2015 to 3,143 followers 2 0

RT @brianfederal: Will the future of Virtual Reality B Mobile? Inside 3D Printing Santa Clara explores the idea #3DPrinting #I3DPConf https…

Inside3DPrint @Inside3DPrint5:46 PM – 21 Oct 2015 to 3,143 followers 2 0

RT @3DPrint_com: On to the Exhibit Hall! Say hi if you see us! Editor-in-Chief Sarah, writer Scott at #I3DPConf #livefromI3DPConf https://t…

Inside3DPrint @Inside3DPrint5:46 PM – 21 Oct 2015 to 3,143 followers 2 0

RT @Forecast3D: What is this #RaceCar we keep talkin about? Stay tuned, we’ll be tweeting pics of each #3DP part during #I3DPConf! #3dprint…

Chris Cleary @chriscleary15:45 PM – 21 Oct 2015 to 315 followers 10 0

RT @SJGrunewald: @GoEngineer 3D printed #halo figure. One piece on #objetconnex @Stratasys multi color and materials. #I3DPConf https://t.c…

Inside3DPrint @Inside3DPrint5:45 PM – 21 Oct 2015 to 3,143 followers 10 0

RT @SJGrunewald: @GoEngineer 3D printed #halo figure. One piece on #objetconnex @Stratasys multi color and materials. #I3DPConf https://t.c…

GoEngineer @GoEngineer5:45 PM – 21 Oct 2015 to 1,689 followers 10 0

RT @SJGrunewald: @GoEngineer 3D printed #halo figure. One piece on #objetconnex @Stratasys multi color and materials. #I3DPConf https://t.c…

seancrotty @seancrotty5:45 PM – 21 Oct 2015 to 117 followers 2 2

Arrived at the Inside 3D Printing Conference in Santa Clara #I3DPConf for innovation & IP strategy opps with fulcrumstrategy.com

iViewing @iViewing5:43 PM – 21 Oct 2015 to 467 followers 3 0

RT @IntraconGlobal: Affordable cardboard viewers will democratize and popularize until higher quality #VR experiences become more economica…

iViewing @iViewing5:43 PM – 21 Oct 2015 to 467 followers 4 0

RT @GreenfishLabs: If u are at the #I3DPConf 2day, check out VR in Mktg and Branding session 2 hear our team talk about #VR in advertising!…

la moustache ringard @SJGrunewald5:42 PM – 21 Oct 2015 to 234 followers 10 9

@GoEngineer 3D printed #halo figure. One piece on #objetconnex @Stratasys multi color and materials. #I3DPConf https://t.co/7Jr9qnYBhv

Edmond Dantes @Edg_Art_ValFlo5:41 PM – 21 Oct 2015 to 69 followers 0 1

#I3DPConf Santa Clara CA

Forecast3D @Forecast3D5:40 PM – 21 Oct 2015 to 172 followers 2 1
What is this #RaceCar we keep talkin about? Stay tuned, we’ll be tweeting pics of each #3DP part during #I3DPConf! #3dprinting #3dprint #mfg

Inside3DPrint @Inside3DPrint5:38 PM – 21 Oct 2015 to 3,143 followers 1 0

RT @aaronilevinson: Know your risk level when you’re manufacturing with #3dprinting #I3DPConf @HuschBlackwell https://t.co/747cVVM0uo

Wim Vanroose @vanroose5:38 PM – 21 Oct 2015 to 28 followers 4 0

RT @sffashiontech: Customization is a loaded term. True custom requires scans, body modeling, vertex matching, more. We’re just scratching …
Wim Vanroose @vanroose5:37 PM – 21 Oct 2015 to 28 followers 2 0

RT @sffashiontech: .@BodyLabsInc offer a richer representation of a person than a scan. Including prediction of body changes #I3DPConf http…

alex@fabbing @fabbing5:34 PM – 21 Oct 2015 to 8,565 followers 2 0

RT @brianfederal: Will the future of Virtual Reality B Mobile? Inside 3D Printing Santa Clara explores the idea #3DPrinting #I3DPConf https…

Inside3DPrint @Inside3DPrint5:32 PM – 21 Oct 2015 to 3,143 followers 1 0

RT @GoEngineer: Come by #GoEngineer Booth #315 at the #I3DPConf today in Santa Clara. @Inside3DPrint

GoEngineer @GoEngineer5:32 PM – 21 Oct 2015 to 1,689 followers 1 1

Come by #GoEngineer Booth #315 at the #I3DPConf today in Santa Clara. @Inside3DPrint

Aaron Levinson @aaronilevinson5:30 PM – 21 Oct 2015 to 57 followers 1 2

Know your risk level when you’re manufacturing with #3dprinting #I3DPConf @HuschBlackwell https://t.co/747cVVM0uo

Inside3DPrint @Inside3DPrint5:30 PM – 21 Oct 2015 to 3,143 followers 3 1

Submit your ?’s for @TheUPSStore panel by 10/22 using #3DPWeek mcklr.me/1LmSvbw #I3DPConf #3dprinting @3d_printers @TerryWohlers

la moustache ringard @SJGrunewald5:28 PM – 21 Oct 2015 to 234 followers 1 3

@ff3dprinters new #flashforge #finder low cost printer available next month. Projected price $699. #I3DPConf https://t.co/zEbyTzFm45

3DPrint @3DPrint_com5:28 PM – 21 Oct 2015 to 33,757 followers 2 3

On to the Exhibit Hall! Say hi if you see us! Editor-in-Chief Sarah, writer Scott at #I3DPConf #livefromI3DPConf https://t.co/eLXG8WsyVm

Aaron Levinson @aaronilevinson5:28 PM – 21 Oct 2015 to 57 followers 2 0

RT @MakerMarketer: Happy back to the future day with @LOkarpas #I3DPconf https://t.co/K8x98OFg8x
Derek Mathers @MakerMarketer5:26 PM – 21 Oct 2015 to 624 followers 4 0
RT @sffashiontech: Customization is a loaded term. True custom requires scans, body modeling, vertex matching, more. We’re just scratching …
Inside3DPrint @Inside3DPrint5:24 PM – 21 Oct 2015 to 3,143 followers 1 0

RT @Avatarium3D: The expo is open – stop by booth #303 at the Inside 3D Printing Expo to see the Avatarium Studio Pro. #I3DPConf https://t.…

Inside3DPrint @Inside3DPrint5:24 PM – 21 Oct 2015 to 3,143 followers 4 0

RT @GreenfishLabs: If u are at the #I3DPConf 2day, check out VR in Mktg and Branding session 2 hear our team talk about #VR in advertising!…

Inside3DPrint @Inside3DPrint5:23 PM – 21 Oct 2015 to 3,143 followers 2 0

RT @sffashiontech: .@BodyLabsInc offer a richer representation of a person than a scan. Including prediction of body changes #I3DPConf http…

Chicago VR @Chicago_VR5:23 PM – 21 Oct 2015 to 433 followers 4 0

RT @GreenfishLabs: If u are at the #I3DPConf 2day, check out VR in Mktg and Branding session 2 hear our team talk about #VR in advertising!…

Inside3DPrint @Inside3DPrint5:23 PM – 21 Oct 2015 to 3,143 followers 3 0

RT @IntraconGlobal: Affordable cardboard viewers will democratize and popularize until higher quality #VR experiences become more economica…

Jon Falker @jfalk5:23 PM – 21 Oct 2015 to 550 followers 2 0

RT @Inside3DPrint: 82% of things that broke on #ISS might be fixed with #additivemanufacturing @RushSpace @MadeInSpace #I3DPConf #space #3…

Inside3DPrint @Inside3DPrint5:23 PM – 21 Oct 2015 to 3,143 followers 4 0

RT @sffashiontech: Customization is a loaded term. True custom requires scans, body modeling, vertex matching, more. We’re just scratching …
Brian Federal @brianfederal5:23 PM – 21 Oct 2015 to 10,290 followers 2 3

Will the future of Virtual Reality B Mobile? Inside 3D Printing Santa Clara explores the idea #3DPrinting #I3DPConf https://t.co/po88XgNRMC

Inside3DPrint @Inside3DPrint5:23 PM – 21 Oct 2015 to 3,143 followers 1 0

RT @Forecast3D: Good morning #SiliconValley! It’s day 1 of #I3DPConf – come see us & our #RaceCar w/ over 45 3DP parts in booth 309! https:…

Michelle Van Slyke @mkvanslyke5:23 PM – 21 Oct 2015 to 244 followers 2 0
RT @TheUPSStore: Tomorrow at #I3DPConf our expert panel is ready to answer your questions! Submit yours using #3DPWeek https://t.co/PsWXws8…

Forecast3D @Forecast3D5:23 PM – 21 Oct 2015 to 172 followers 1 1
Good morning #SiliconValley! It’s day 1 of #I3DPConf – come see us & our #RaceCar w/ over 45 3DP parts in booth 309! https://t.co/EZA4GQN5eu
Boise VR Project @Boisevrproject5:22 PM – 21 Oct 2015 to 119 followers 3 0

RT @IntraconGlobal: Affordable cardboard viewers will democratize and popularize until higher quality #VR experiences become more economica…

Boise VR Project @Boisevrproject5:22 PM – 21 Oct 2015 to 119 followers 1 0

RT @IntraconGlobal: The #virtualreality kickoff panel discusses the mobile future of #VR. #I3DPConf https://t.co/LVUdJDuFVz

Bazooka Tech @bazooka_tech5:21 PM – 21 Oct 2015 to 1,173 followers 2 0
RT @brianfederal: Andrew with Made in Space talks #Robotics and #3DPrinting to manufacture in Space @ Inside 3D Printing #I3DPConf https://…

Bazooka Tech @bazooka_tech5:21 PM – 21 Oct 2015 to 1,173 followers 3 0
RT @RealityInc: Then @madeinspace will 3D print cubesats in space. #3dprinting #I3DPConf https://t.co/jHIcvFa2CA

SF Fashtech @sffashiontech5:21 PM – 21 Oct 2015 to 2,679 followers 4 3

Customization is a loaded term. True custom requires scans, body modeling, vertex matching, more. We’re just scratching surface. #I3DPConf

Inside3DPrint @Inside3DPrint5:16 PM – 21 Oct 2015 to 3,143 followers 2 0

RT @RealityInc: .@MadeInSpace is working on #3dprinting on the moon too! #I3DPConf https://t.co/s5w9QKuFlD
Inside3DPrint @Inside3DPrint5:16 PM – 21 Oct 2015 to 3,143 followers 0 0

.@LOKarpas of @Metamason3D answers audience questions on #metamodernism #I3DPConf https://t.co/TJHVBR2sgd

la moustache ringard @SJGrunewald5:15 PM – 21 Oct 2015 to 234 followers 1 3

#raise3d on #Kickstarter can print 85 degree overhang. 10 materials. Extruded temp 300 degrees. #I3DPConf https://t.co/zsXXirXKag

Inside3DPrint @Inside3DPrint5:15 PM – 21 Oct 2015 to 3,143 followers 3 0

RT @RealityInc: Then @madeinspace will 3D print cubesats in space. #3dprinting #I3DPConf https://t.co/jHIcvFa2CA

Scansite @Scansite3D5:15 PM – 21 Oct 2015 to 13 followers 0 2
We made it to #I3DPConf! #3dprinting #3dprinting https://t.co/qlFM1m5Diu

Inside3DPrint @Inside3DPrint5:15 PM – 21 Oct 2015 to 3,143 followers 2 0

RT @MakerMarketer: Happy back to the future day with @LOkarpas #I3DPconf https://t.co/K8x98OFg8x
Intracon @IntraconGlobal5:13 PM – 21 Oct 2015 to 116 followers 3 3

Affordable cardboard viewers will democratize and popularize until higher quality #VR experiences become more economically viable. #I3DPConf

Intracon @IntraconGlobal5:13 PM – 21 Oct 2015 to 116 followers 1 3

The #virtualreality kickoff panel discusses the mobile future of #VR. #I3DPConf https://t.co/LVUdJDuFVz
NYC Health Business @NYCHBL5:10 PM – 21 Oct 2015 to 1,655 followers 1 0
RT @BunnyEllerin: thanks for the nice shout out for organizing medical track #i3dpconf Very nice 🙂
Rapid Prototyping @rapidprototypin5:08 PM – 21 Oct 2015 to 3,396 followers 1 0

RT @RealityInc: A PhD in VR 20 yrs ago was career suicide #I3DPConf #3Dprinting https://t.co/zzFl4dFtVH
Rapid Prototyping @rapidprototypin5:08 PM – 21 Oct 2015 to 3,396 followers 1 0

RT @RealityInc: Getting my VR swerve on at #I3DPConf #3Dprinting https://t.co/5CacMCgXw3
Rapid Prototyping @rapidprototypin5:08 PM – 21 Oct 2015 to 3,396 followers 1 0

RT @RealityInc: No supports in space! Too dangerous says @MadeInSpace #3Dprinting #I3DPConf https://t.co/3oGf5Hq41S

SF Fashtech @sffashiontech5:08 PM – 21 Oct 2015 to 2,679 followers 2 3

.@BodyLabsInc offer a richer representation of a person than a scan. Including prediction of body changes #I3DPConf https://t.co/DT97rnduYC

John Meckler @johnmeckler5:07 PM – 21 Oct 2015 to 325 followers 2 0

RT @Inside3DPrint: 82% of things that broke on #ISS might be fixed with #additivemanufacturing @RushSpace @MadeInSpace #I3DPConf #space #3…

Avatarium 3D @Avatarium3D5:07 PM – 21 Oct 2015 to 47 followers 1 1

The expo is open – stop by booth #303 at the Inside 3D Printing Expo to see the Avatarium Studio Pro. #I3DPConf https://t.co/RgDgSDmGPR

NYC Health Business @NYCHBL5:07 PM – 21 Oct 2015 to 1,655 followers 2 0
RT @Inside3DPrint: “Current #3dprinting is like computers 40 yrs ago” @JDogru, @3DPrinterOS #I3DPConf https://t.co/gp29z9515H

Greenfish Labs @GreenfishLabs5:06 PM – 21 Oct 2015 to 690 followers 4 3

If u are at the #I3DPConf 2day, check out VR in Mktg and Branding session 2 hear our team talk about #VR in advertising! #VirtualReality

alex@fabbing @fabbing5:04 PM – 21 Oct 2015 to 8,565 followers 2 0

RT @brianfederal: Andrew with Made in Space talks #Robotics and #3DPrinting to manufacture in Space @ Inside 3D Printing #I3DPConf https://…

Inside3DPrint @Inside3DPrint5:03 PM – 21 Oct 2015 to 3,143 followers 2 3

“Current #3dprinting is like computers 40 yrs ago” @JDogru, @3DPrinterOS #I3DPConf https://t.co/gp29z9515H
3DPrint @3DPrint_com5:02 PM – 21 Oct 2015 to 33,757 followers 0 1

The Shape of Things to Come, sponsored session from Husch Blackwell, hits on IP & more #I3DPConf #livefromI3DPConf https://t.co/2rU2XVMp0t

The UPS Store @TheUPSStore5:02 PM – 21 Oct 2015 to 11,688 followers 2 10
Tomorrow at #I3DPConf our expert panel is ready to answer your questions! Submit yours using #3DPWeek pic.twitter.com/PsWXws8x5Y

Inside3DPrint @Inside3DPrint5:01 PM – 21 Oct 2015 to 3,143 followers 1 0

RT @sffashiontech: Checking out the world of #3DPrinting and #VR at @Inside3DPrint! #I3DPConf

Inside3DPrint @Inside3DPrint5:00 PM – 21 Oct 2015 to 3,143 followers 2 1

Crowded room for @HuschBlackwell’s sponsored session at #I3DPConf Santa Clara #3dprinting https://t.co/CaCU7kYBWy

SF Fashtech @sffashiontech5:00 PM – 21 Oct 2015 to 2,679 followers 1 2

Checking out the world of #3DPrinting and #VR at @Inside3DPrint! #I3DPConf

The UPS Store @TheUPSStore5:00 PM – 21 Oct 2015 to 11,688 followers 1 5
Remember to submit your questions for @TerryWohlers & @3d_printers of experts at #I3DPConf using #3DPWeek! https://t.co/IqBukWh2Im

Inside3DPrint @Inside3DPrint4:56 PM – 21 Oct 2015 to 3,143 followers 1 5

.@StewQ asks #I3DPConf 1st timers about what brought them here. If you’re in Santa Clara, what led you to attend? https://t.co/sRuAIlTxbg
Derek Mathers @MakerMarketer4:55 PM – 21 Oct 2015 to 624 followers 3 0
RT @RealityInc: Then @madeinspace will 3D print cubesats in space. #3dprinting #I3DPConf https://t.co/jHIcvFa2CA

Derek Mathers @MakerMarketer4:54 PM – 21 Oct 2015 to 624 followers 2 2
Happy back to the future day with @LOkarpas #I3DPconf https://t.co/K8x98OFg8x

Reality™ Institute @RealityInc4:52 PM – 21 Oct 2015 to 534 followers 1 0

A PhD in VR 20 yrs ago was career suicide #I3DPConf #3Dprinting https://t.co/zzFl4dFtVH

Reality™ Institute @RealityInc4:50 PM – 21 Oct 2015 to 534 followers 1 2

Getting my VR swerve on at #I3DPConf #3Dprinting https://t.co/5CacMCgXw3

Reality™ Institute @RealityInc4:49 PM – 21 Oct 2015 to 534 followers 1 0

No supports in space! Too dangerous says @MadeInSpace #3Dprinting #I3DPConf https://t.co/3oGf5Hq41S
la moustache ringard @SJGrunewald4:48 PM – 21 Oct 2015 to 234 followers 2 1

@MadeInSpace Working on recycling materials, working w/ conductives, metal materials & exploring #3dprinting moon base. #I3DPConf

Tamara @MeridianMOD4:47 PM – 21 Oct 2015 to 138 followers 0 0

3D me! Avatarium 3D #3dprintingexpo #I3DPConf #avatarium3d

Aaron Levinson @aaronilevinson4:46 PM – 21 Oct 2015 to 57 followers 2 0

RT @3DPrint_com: “Recycling is key,” says Made in Space president, for “Earth-independent production.” #I3DPConf #livefromI3DPConf

Mara Hitner @3DPGirl4:46 PM – 21 Oct 2015 to 120 followers 0 3

No #HoverBoard but we can #3DPrint stuff in space! @MadeInSpace #I3DPConf #BackToFutureDay #We’reInTheFuture https://t.co/ruT5B12Y7F

Aaron Levinson @aaronilevinson4:42 PM – 21 Oct 2015 to 57 followers 2 0

RT @RealityInc: .@MadeInSpace is working on #3dprinting on the moon too! #I3DPConf https://t.co/s5w9QKuFlD
Aaron Levinson @aaronilevinson4:41 PM – 21 Oct 2015 to 57 followers 0 0

3D printing in microgravity would have really helped Mark Watney @andyweirauthor @MartianMovie @MadeInSpace #I3DPConf

Intracon @IntraconGlobal4:41 PM – 21 Oct 2015 to 116 followers 0 1

#3Dprinting is crucial to Earth-independent production, since models/parts can be recycled for filament. #I3DPConf https://t.co/XZlXmN3r6u

Reality™ Institute @RealityInc4:41 PM – 21 Oct 2015 to 534 followers 2 1

.@MadeInSpace is working on #3dprinting on the moon too! #I3DPConf https://t.co/s5w9QKuFlD
Inside3DPrint @Inside3DPrint4:40 PM – 21 Oct 2015 to 3,143 followers 0 0

In 20 min: @HuschBlackwell Sponsored Session, success in #3dprinting age! mcklr.me/1Lluhyp #I3DPConf https://t.co/NVNAewNDUG

Boise VR Project @Boisevrproject4:39 PM – 21 Oct 2015 to 119 followers 1 0

RT @IntraconGlobal: Gravity-free @MadeInSpace #3Dprinting is already helping at the International @space_station. #I3DPConf https://t.co/L3…

Brian Federal @brianfederal4:38 PM – 21 Oct 2015 to 10,290 followers 0 1

@lulzbot3D check out Lulzbot @ Inside 3D Printing Santa Clara #I3DPConf #3DPrinting #OpenSource #3DPrinter #3DPrint

3DPrint @3DPrint_com4:36 PM – 21 Oct 2015 to 33,757 followers 2 3

“Recycling is key,” says Made in Space president, for “Earth-independent production.” #I3DPConf #livefromI3DPConf

Reality™ Institute @RealityInc4:35 PM – 21 Oct 2015 to 534 followers 0 0

.@MadeInSpace hopes to recycle parts with feedstock extruder #3Dprinting #I3DPConf https://t.co/e0ThpULkvR
Reality™ Institute @RealityInc4:34 PM – 21 Oct 2015 to 534 followers 0 0

.@MadeInSpace’s next printer prints in low-temp metal #3dprinting #I3DPConf https://t.co/JDgnZt2cwY
Danielle Matich @DanielleMatich4:34 PM – 21 Oct 2015 to 86 followers 1 0

RT @RealityInc: Up next from @madeinspace: multi material #3dprinting in space #I3DPConf https://t.co/scv3zbrWhD
alex@fabbing @fabbing4:34 PM – 21 Oct 2015 to 8,565 followers 1 0

RT @brianfederal: Inside 3D Printing kicks off with Stuart intro| Made in Space #MadeinSpace #SpaceX #ElonMusk #I3DPConf #3DPrinting https:…

Danielle Matich @DanielleMatich4:34 PM – 21 Oct 2015 to 86 followers 1 0

RT @RealityInc: The ISS is shutting down in 2024?? #I3DPConf #3dprinting

Brian Federal @brianfederal4:34 PM – 21 Oct 2015 to 10,290 followers 2 0

Andrew with Made in Space talks #Robotics and #3DPrinting to manufacture in Space @ Inside 3D Printing #I3DPConf https://t.co/ufsR8PAanJ

Intracon @IntraconGlobal4:33 PM – 21 Oct 2015 to 116 followers 1 1

Gravity-free @MadeInSpace #3Dprinting is already helping at the International @space_station. #I3DPConf https://t.co/L3ikwdLjMT

Tyler Benster @tbenst4:32 PM – 21 Oct 2015 to 454 followers 0 1
“Imagine if someone said ‘in less than a decade the industry your business is based on won’t be around'” @MadeInSpace #savetheISS #I3DPConf

Reality™ Institute @RealityInc4:31 PM – 21 Oct 2015 to 534 followers 0 1

The ISS has businesses tied to it that @MadeInSpace wants to save with #3Dprinting #I3DPConf https://t.co/sDr1SAWyGV

Reality™ Institute @RealityInc4:30 PM – 21 Oct 2015 to 534 followers 1 0

The ISS is shutting down in 2024?? #I3DPConf #3dprinting

Reality™ Institute @RealityInc4:30 PM – 21 Oct 2015 to 534 followers 0 0

Then, #3dprinting cubesats in space. #I3DPConf @MadeInSpace https://t.co/KKfCFLgDIE

Reality™ Institute @RealityInc4:27 PM – 21 Oct 2015 to 534 followers 1 0

Up next from @madeinspace: multi material #3dprinting in space #I3DPConf https://t.co/scv3zbrWhD
Reality™ Institute @RealityInc4:27 PM – 21 Oct 2015 to 534 followers 3 2

Then @madeinspace will 3D print cubesats in space. #3dprinting #I3DPConf https://t.co/jHIcvFa2CA
Danielle Matich @DanielleMatich4:26 PM – 21 Oct 2015 to 86 followers 2 0

RT @Inside3DPrint: 1st KEYNOTE of #I3DPConf starts NOW w/ @RushSpace, @MadeInSpace mcklr.me/1Pukco6 #3dprinting #space https://t.co/…

Danielle Matich @DanielleMatich4:25 PM – 21 Oct 2015 to 86 followers 2 0

RT @RealityInc: “A 3D printer is a meta tool” @madeinspace at #I3DPConf #3dprinting #AwesomeTech https://t.co/FZhbqHE7Mt

Stewart Quealy @StewQ4:24 PM – 21 Oct 2015 to 8,204 followers 1 0
RT @BunnyEllerin: aha didn’t realize @stewq was on twitter – so thanks for the nice shout out at @Inside3Dprint Santa Clara #I3DPConf

Inside3DPrint @Inside3DPrint4:24 PM – 21 Oct 2015 to 3,143 followers 0 0

We can digitally send things to #space an order of magnitude faster than a traditional rocket @RushSpace #I3DPConf https://t.co/KCuNi77q1C

Intracon @IntraconGlobal4:23 PM – 21 Oct 2015 to 116 followers 0 1

View from the @madeinspace keynote at #I3DPConf. https://t.co/vcRoV8Po7S

Brian Federal @brianfederal4:20 PM – 21 Oct 2015 to 10,290 followers 1 0

Inside 3D Printing kicks off with Stuart intro| Made in Space #MadeinSpace #SpaceX #ElonMusk #I3DPConf #3DPrinting https://t.co/6PeSDGQnVR

Stewart Quealy @StewQ4:19 PM – 21 Oct 2015 to 8,204 followers 2 0
RT @tbenst: Andrew Rush, @MadeInSpace kicking off opening keynote of #I3DPConf Santa Clara! “geeks who want to live off planet” https://t.c…
Stewart Quealy @StewQ4:18 PM – 21 Oct 2015 to 8,204 followers 1 1
Stellar opening keynote with Andrew Rush of @MadeInSpace #I3DPConf https://t.co/o4oOKUmXB4
Inside3DPrint @Inside3DPrint4:17 PM – 21 Oct 2015 to 3,143 followers 2 0

RT @tbenst: Andrew Rush, @MadeInSpace kicking off opening keynote of #I3DPConf Santa Clara! “geeks who want to live off planet” https://t.c…
Inside3DPrint @Inside3DPrint4:17 PM – 21 Oct 2015 to 3,143 followers 2 0

RT @RealityInc: “A 3D printer is a meta tool” @madeinspace at #I3DPConf #3dprinting #AwesomeTech https://t.co/FZhbqHE7Mt

Inside3DPrint @Inside3DPrint4:16 PM – 21 Oct 2015 to 3,143 followers 2 0

82% of things that broke on #ISS might be fixed with #additivemanufacturing @RushSpace @MadeInSpace #I3DPConf #space #3dprinting

Tyler Benster @tbenst4:15 PM – 21 Oct 2015 to 454 followers 3 0
RT @AmericaMakes: For those at #AMmember @Inside3DPrint’s #I3DPConf, check out their list of speakers: bit.ly/1XhT7XB

Reality™ Institute @RealityInc4:13 PM – 21 Oct 2015 to 534 followers 2 1

“A 3D printer is a meta tool” @madeinspace at #I3DPConf #3dprinting #AwesomeTech https://t.co/FZhbqHE7Mt
Tyler Benster @tbenst4:13 PM – 21 Oct 2015 to 454 followers 2 1
Andrew Rush, @MadeInSpace kicking off opening keynote of #I3DPConf Santa Clara! “geeks who want to live off planet” https://t.co/L2seTrLkKk
Bazooka Tech @bazooka_tech4:12 PM – 21 Oct 2015 to 1,173 followers 2 0
RT @3Dprintbeginner: CONFIRMED: @divbyztech to exhibit at @Inside3DPrint ‘s #I3DPConf Mumbai mcklr.me/1KNhDau #3dprinting

Proto-pasta @Proto_pasta4:10 PM – 21 Oct 2015 to 1,040 followers 4 0

RT @lulzbot3D: If you’re at #I3DPConf in Santa Clara, stop by booth 505! We’re hanging out with @Proto_pasta, too! @Inside3DPrint #3dprinti…

Inside3DPrint @Inside3DPrint4:10 PM – 21 Oct 2015 to 3,143 followers 0 0

Hod Lisbon, @columbia introduces Andrew Rush, @MadeInSpace @RushSpace #I3DPConf https://t.co/sAW2aKmdhx

Inside3DPrint @Inside3DPrint4:09 PM – 21 Oct 2015 to 3,143 followers 4 0

RT @lulzbot3D: If you’re at #I3DPConf in Santa Clara, stop by booth 505! We’re hanging out with @Proto_pasta, too! @Inside3DPrint #3dprinti…

LulzBot @lulzbot3D4:08 PM – 21 Oct 2015 to 2,784 followers 4 5

If you’re at #I3DPConf in Santa Clara, stop by booth 505! We’re hanging out with @Proto_pasta, too! @Inside3DPrint #3dprinting

Bunny Ellerin @BunnyEllerin4:08 PM – 21 Oct 2015 to 2,450 followers 1 5

aha didn’t realize @stewq was on twitter – so thanks for the nice shout out at @Inside3Dprint Santa Clara #I3DPConf

Reality™ Institute @RealityInc4:08 PM – 21 Oct 2015 to 534 followers 2 0

RT @Inside3DPrint: 1st KEYNOTE of #I3DPConf starts NOW w/ @RushSpace, @MadeInSpace mcklr.me/1Pukco6 #3dprinting #space https://t.co/…

Inside3DPrint @Inside3DPrint4:07 PM – 21 Oct 2015 to 3,143 followers 0 0

39 states and 34 countries represented at #I3DPConf Santa Clara #3dprinting

Bunny Ellerin @BunnyEllerin4:07 PM – 21 Oct 2015 to 2,450 followers 1 2

thanks for the nice shout out for organizing medical track #i3dpconf Very nice 🙂

Rapid Prototyping @rapidprototypin4:03 PM – 21 Oct 2015 to 3,396 followers 2 0

RT @3Dprintbeginner: CONFIRMED: @divbyztech to exhibit at @Inside3DPrint ‘s #I3DPConf Mumbai mcklr.me/1KNhDau #3dprinting

Inside3DPrint @Inside3DPrint4:00 PM – 21 Oct 2015 to 3,143 followers 2 0

1st KEYNOTE of #I3DPConf starts NOW w/ @RushSpace, @MadeInSpace mcklr.me/1Pukco6 #3dprinting #space https://t.co/LUTuhvJOGb

3DPrint @3DPrint_com3:57 PM – 21 Oct 2015 to 33,757 followers 0 1

Here we go! Morning keynote from #MadeinSpace #I3DPConf #livefromI3DPConf https://t.co/FXIKWK6LWY
la moustache ringard @SJGrunewald3:49 PM – 21 Oct 2015 to 234 followers 0 2

First on today’s agenda. Opening keynote from Andrew Rush and @MadeInSpace #I3DPConf

Start 3D Printing @3Dprintbeginner3:46 PM – 21 Oct 2015 to 2,989 followers 2 2

CONFIRMED: @divbyztech to exhibit at @Inside3DPrint ‘s #I3DPConf Mumbai mcklr.me/1KNhDau #3dprinting

Keith Avery @unkeiqo3:35 PM – 21 Oct 2015 to 61 followers 3 0

RT @AmericaMakes: For those at #AMmember @Inside3DPrint’s #I3DPConf, check out their list of speakers: bit.ly/1XhT7XB

la moustache ringard @SJGrunewald3:25 PM – 21 Oct 2015 to 234 followers 0 0

It’s time for #i3dpconf #livefrom3DPconf lets get our 3D print on. https://t.co/n4VRnZl8xF

3D Print Week CA @3dprintweekca3:15 PM – 21 Oct 2015 to 81 followers 0 0

Welcome to Day 2 of #I3DPConf Santa Clara! Exhibit hall opens at 10AM mcklr.me/1G6I8vL #3dprinting https://t.co/XIUg6YYvRE

Inside3DPrint @Inside3DPrint3:15 PM – 21 Oct 2015 to 3,143 followers 0 3

Welcome to Day 2 of #I3DPConf Santa Clara! Exhibit hall opens at 10AM mcklr.me/1G6I8vL #3dprinting https://t.co/4ceUI3EFkV

Inside3DPrint @Inside3DPrint3:09 PM – 21 Oct 2015 to 3,143 followers 3 0

RT @AmericaMakes: For those at #AMmember @Inside3DPrint’s #I3DPConf, check out their list of speakers: bit.ly/1XhT7XB

America Makes @AmericaMakes3:00 PM – 21 Oct 2015 to 4,938 followers 3 2

For those at #AMmember @Inside3DPrint’s #I3DPConf, check out their list of speakers: bit.ly/1XhT7XB

C.R.S. @ribeiroharman2:51 PM – 21 Oct 2015 to 1,562 followers 1 0

RT @3DPrint_com: Good morning, Santa Clara! #I3DPConf #livefromI3DPConf https://t.co/8xaWwDTZBT
3DPrint @3DPrint_com2:47 PM – 21 Oct 2015 to 33,757 followers 1 1

Good morning, Santa Clara! #I3DPConf #livefromI3DPConf https://t.co/8xaWwDTZBT

Inside3DPrint @Inside3DPrint2:38 PM – 21 Oct 2015 to 3,143 followers 3 0

RT @TommyKeiser: Stop by booth #203 @Inside3DPrint meet @StoopidBuddy Digital team & see @SuperMansiontv characters brought to life w/ @Env…

EnvisionTEC, Inc. @EnvisionTEC2:07 PM – 21 Oct 2015 to 2,207 followers 3 0

RT @TommyKeiser: Stop by booth #203 @Inside3DPrint meet @StoopidBuddy Digital team & see @SuperMansiontv characters brought to life w/ @Env…

david a quinley @davidaquinley1:57 PM – 21 Oct 2015 to 4,506 followers 1 0

RT @Inside3DPrint: What was your favorite tutorial of #I3DPConf Santa Clara Day 1? RT & tell us! mcklr.me/1PncuwV #3dprinting https:…

IRiS 3D Solutions @iris3dsolutions8:29 AM – 21 Oct 2015 to 4,649 followers 2 0

RT @My3DPrinting: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https:…

Bahaa Abu Nojaim @Baha_AbuNojaim5:58 AM – 21 Oct 2015 to 216 followers 4 0

RT @Inside3DPrint: Thanks @MakePrintable for revealing our fortune! #3dprinting #I3DPConf https://t.co/2vdbpZrQC1

Mo Taslaq @muhannadtaslaq5:58 AM – 21 Oct 2015 to 2,133 followers 4 0

RT @Inside3DPrint: Thanks @MakePrintable for revealing our fortune! #3dprinting #I3DPConf https://t.co/2vdbpZrQC1

Zeb Wells @zebwells4:42 AM – 21 Oct 2015 to 3,103 followers 3 0

RT @TommyKeiser: Stop by booth #203 @Inside3DPrint meet @StoopidBuddy Digital team & see @SuperMansiontv characters brought to life w/ @Env…

Tommy Keiser @TommyKeiser3:54 AM – 21 Oct 2015 to 223 followers 3 2

Stop by booth #203 @Inside3DPrint meet @StoopidBuddy Digital team & see @SuperMansiontv characters brought to life w/ @EnvisionTEC #I3DPConf

Eternal Buzz @jimmitchell703:51 AM – 21 Oct 2015 to 817 followers 9 0

RT @3DPrintingFans: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… http…

Computer Print News @Recycling_Times3:45 AM – 21 Oct 2015 to 15,000 followers 0 0

Follow us on #Pinterest to get the latest pictures from the #RWE2015 and #I3DPConf pinterest.com/InTouchNews/ https://t.co/GRFEVKK0a1

3D Printing @Just_Print_It2:54 AM – 21 Oct 2015 to 839 followers 9 0

RT @3DPrintingFans: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… http…

Dynamic 3D Printing @Dynamic3DP2:47 AM – 21 Oct 2015 to 1,306 followers 9 0

RT @tbenst: Beautiful titanium print by @3dsystemscorp #I3DPConf https://t.co/Ie21Cv0tls

Mike D @mdfast12:28 AM – 21 Oct 2015 to 47 followers 4 0

RT @formlabs: Headed to 3D Printing Santa Clara today? Don’t forget to stop by booth 502 to see the new Form 2! @Inside3DPrint #I3DPConf #3…

THINKTANKREPAIR @ThinkTankRepair2:09 AM – 21 Oct 2015 to 94 followers 4 0

RT @Inside3DPrint: Thanks @MakePrintable for revealing our fortune! #3dprinting #I3DPConf https://t.co/2vdbpZrQC1

Potomac Photonics @PotomacPhotonic1:48 AM – 21 Oct 2015 to 69,582 followers 9 0

RT @tbenst: Beautiful titanium print by @3dsystemscorp #I3DPConf https://t.co/Ie21Cv0tls

MakePrintable @MakePrintable1:30 AM – 21 Oct 2015 to 31 followers 4 0

RT @Inside3DPrint: Thanks @MakePrintable for revealing our fortune! #3dprinting #I3DPConf https://t.co/2vdbpZrQC1

Inside3DPrint @Inside3DPrint1:30 AM – 21 Oct 2015 to 3,143 followers 1 0

What was your favorite tutorial of #I3DPConf Santa Clara Day 1? RT & tell us! mcklr.me/1PncuwV #3dprinting https://t.co/5zlnKOG1Wq

John Hornick @JHornick3D1Stop1:20 AM – 21 Oct 2015 to 313 followers 3 0

RT @Inside3DPrint: “Laser melting, where you see most of the alphabet soup” – @JHornick3D1Stop, @FinneganIPLaw #powderbedfusion #I3DPConf

Inside3DPrint @Inside3DPrint1:19 AM – 21 Oct 2015 to 3,143 followers 4 5

Thanks @MakePrintable for revealing our fortune! #3dprinting #I3DPConf https://t.co/2vdbpZrQC1
Stewart Quealy @StewQ12:40 AM – 21 Oct 2015 to 8,204 followers 3 0
RT @brianfederal: Inside 3D Printing #HP displays Sprout #I3DPConf #3dprinting https://t.co/USj7LDeyOg
Stewart Quealy @StewQ12:40 AM – 21 Oct 2015 to 8,204 followers 9 0
RT @tbenst: Beautiful titanium print by @3dsystemscorp #I3DPConf https://t.co/Ie21Cv0tls

Stewart Quealy @StewQ12:40 AM – 21 Oct 2015 to 8,204 followers 4 0
RT @Inside3DPrint: Packed room for Hod Lipson’s intro to #3dprinting #I3DPConf https://t.co/GjISlW3nSR
3D Print Week CA @3dprintweekca12:29 AM – 21 Oct 2015 to 81 followers 4 0

RT @Inside3DPrint: Packed room for Hod Lipson’s intro to #3dprinting #I3DPConf https://t.co/GjISlW3nSR
3D Print Week CA @3dprintweekca12:29 AM – 21 Oct 2015 to 81 followers 2 0

RT @Inside3DPrint: Turlif Vilbrandt passes around some #3dprint creations #I3DPConf @Uformia https://t.co/7J3WqFRHjh

Bazooka Tech @bazooka_tech12:18 AM – 21 Oct 2015 to 1,173 followers 4 0
RT @brianfederal: Hod Lipson speaking now in Santa Clara @ Inside 3D Printing. Awesome! #3DPrinting #I3DPConf #3Dprinter https://t.co/SterA…

Uformia @Uformia12:10 AM – 21 Oct 2015 to 411 followers 2 0

RT @Inside3DPrint: Turlif Vilbrandt passes around some #3dprint creations #I3DPConf @Uformia https://t.co/7J3WqFRHjh

Inside3DPrint @Inside3DPrint12:10 AM – 21 Oct 2015 to 3,143 followers 2 3

Turlif Vilbrandt passes around some #3dprint creations #I3DPConf @Uformia https://t.co/7J3WqFRHjh
B3dge @Build3dprinter11:59 PM – 20 Oct 2015 to 74,839 followers 4 0

RT @brianfederal: Hod Lipson speaking now in Santa Clara @ Inside 3D Printing. Awesome! #3DPrinting #I3DPConf #3Dprinter https://t.co/SterA…

MetalForming Mag @MetalForming11:58 PM – 20 Oct 2015 to 8,581 followers 4 0

RT @Inside3DPrint: Packed room for Hod Lipson’s intro to #3dprinting #I3DPConf https://t.co/GjISlW3nSR
Andrew Flando @AF_metalformag11:57 PM – 20 Oct 2015 to 98 followers 4 0

RT @Inside3DPrint: Packed room for Hod Lipson’s intro to #3dprinting #I3DPConf https://t.co/GjISlW3nSR
Uformia @Uformia11:55 PM – 20 Oct 2015 to 411 followers 1 0

RT @Inside3DPrint: And @uformia’s Turlif Vilbrandt’s session on designing materiality #3dprinting #design #I3DPConf https://t.co/VCG1qhqr4b

alex@fabbing @fabbing11:54 PM – 20 Oct 2015 to 8,565 followers 4 0

RT @brianfederal: Hod Lipson speaking now in Santa Clara @ Inside 3D Printing. Awesome! #3DPrinting #I3DPConf #3Dprinter https://t.co/SterA…

Inside3DPrint @Inside3DPrint11:54 PM – 20 Oct 2015 to 3,143 followers 1 3

And @uformia’s Turlif Vilbrandt’s session on designing materiality #3dprinting #design #I3DPConf https://t.co/VCG1qhqr4b

Andrew Weatherall @AndyDW_11:54 PM – 20 Oct 2015 to 1,755 followers 9 0

RT @tbenst: Beautiful titanium print by @3dsystemscorp #I3DPConf https://t.co/Ie21Cv0tls

Inside3DPrint @Inside3DPrint11:52 PM – 20 Oct 2015 to 3,143 followers 4 1

Packed room for Hod Lipson’s intro to #3dprinting #I3DPConf https://t.co/GjISlW3nSR

o3dprint.ru @o3dprintru11:46 PM – 20 Oct 2015 to 2,600 followers 4 0

RT @brianfederal: Hod Lipson speaking now in Santa Clara @ Inside 3D Printing. Awesome! #3DPrinting #I3DPConf #3Dprinter https://t.co/SterA…

Inside3DPrint @Inside3DPrint11:43 PM – 20 Oct 2015 to 3,143 followers 0 0

.@Columbia’s Hod Lipson: #3dprinting worms its way into all areas, from #ballet to #bioprinted knees. #I3DPConf

Brian Federal @brianfederal11:29 PM – 20 Oct 2015 to 10,290 followers 4 1

Hod Lipson speaking now in Santa Clara @ Inside 3D Printing. Awesome! #3DPrinting #I3DPConf #3Dprinter https://t.co/SterAnQ72N

Rapid Prototyping @rapidprototypin11:26 PM – 20 Oct 2015 to 3,396 followers 3 0

RT @WYNIT3D: Setting up the booth for @Inside3DPrint #3dprinting #i3dpconf Come by and see us tomorrow and Thursday! @Leapfrog_3D https://t…

iViewing @iViewing11:20 PM – 20 Oct 2015 to 467 followers 2 0

RT @ClementAdert: RT Lets see whats new w #3dprinting, for sure excited to see how the #vr topics will be presented #I3DPConf https://t.co/…

Yerald max @painterXman11:16 PM – 20 Oct 2015 to 61 followers 3 0

RT @3DPrintBoard: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https:…

Inside3DPrint @Inside3DPrint11:15 PM – 20 Oct 2015 to 3,143 followers 0 0

NOW: intro to #3dprinting w/ @Columbia’s Hod Lipson mcklr.me/1Ozi0fV #I3DPConf https://t.co/wPPWbA3L3U
犬速 @DOGSPEED11:01 PM – 20 Oct 2015 to 1,488 followers 9 0
RT @tbenst: Beautiful titanium print by @3dsystemscorp #I3DPConf https://t.co/Ie21Cv0tls

Inside3DPrint @Inside3DPrint11:01 PM – 20 Oct 2015 to 3,143 followers 2 0

RT @ClementAdert: RT Lets see whats new w #3dprinting, for sure excited to see how the #vr topics will be presented #I3DPConf https://t.co/…

Inside3DPrint @Inside3DPrint10:55 PM – 20 Oct 2015 to 3,143 followers 3 0

RT @WYNIT3D: Setting up the booth for @Inside3DPrint #3dprinting #i3dpconf Come by and see us tomorrow and Thursday! @Leapfrog_3D https://t…

Teacher with #VR @ClementAdert10:53 PM – 20 Oct 2015 to 275 followers 2 1

RT Lets see whats new w #3dprinting, for sure excited to see how the #vr topics will be presented #I3DPConf https://t.co/HInd7TBInl

Raymond Kramer @RaymondKramer10:50 PM – 20 Oct 2015 to 135 followers 0 3

Lets see whats new w #3dprinting, for sure excited to see how the #vr topics will be presented #I3DPConf

WYNIT 3D @WYNIT3D10:42 PM – 20 Oct 2015 to 259 followers 3 2

Setting up the booth for @Inside3DPrint #3dprinting #i3dpconf Come by and see us tomorrow and Thursday! @Leapfrog_3D https://t.co/hViyUFVUsQ

BTodfield @BlaineTodfield10:38 PM – 20 Oct 2015 to 371 followers 9 0

RT @tbenst: Beautiful titanium print by @3dsystemscorp #I3DPConf https://t.co/Ie21Cv0tls

Andrew Flando @AF_metalformag10:37 PM – 20 Oct 2015 to 98 followers 9 0

RT @tbenst: Beautiful titanium print by @3dsystemscorp #I3DPConf https://t.co/Ie21Cv0tls

Inside3DPrint @Inside3DPrint10:33 PM – 20 Oct 2015 to 3,143 followers 3 0

RT @brianfederal: Inside 3D Printing #HP displays Sprout #I3DPConf #3dprinting https://t.co/USj7LDeyOg
Inside3DPrint @Inside3DPrint10:32 PM – 20 Oct 2015 to 3,143 followers 1 0

RT @Avatarium3D: We’re busy setting up at the 3D Printing Conference & Expo. Stop by booth #303 tomorrow and be wowed! #I3DPConf https://t.…

Inside3DPrint @Inside3DPrint10:32 PM – 20 Oct 2015 to 3,143 followers 3 0

RT @onwevr: Also, if you’re at #I3DPConf in Santa Clara on Oct 21, check @auradeluxe’s panel “Will the Future of VR be Mobile?” https://t.c…
Inside3DPrint @Inside3DPrint10:32 PM – 20 Oct 2015 to 3,143 followers 9 0

RT @tbenst: Beautiful titanium print by @3dsystemscorp #I3DPConf https://t.co/Ie21Cv0tls

Mara Hitner @3DPGirl10:21 PM – 20 Oct 2015 to 120 followers 0 1

OMW to #I3DPConf! If I’m smiling for no reason it’s cuz @Lin_Manuel n @HamiltonMusical cast in my head screaming “LafaYETTE!” #YayHamlet

Tony Parisi @auradeluxe10:06 PM – 20 Oct 2015 to 2,477 followers 3 0

RT @onwevr: Also, if you’re at #I3DPConf in Santa Clara on Oct 21, check @auradeluxe’s panel “Will the Future of VR be Mobile?” https://t.c…
Tyler Benster @tbenst10:04 PM – 20 Oct 2015 to 454 followers 9 6
Beautiful titanium print by @3dsystemscorp #I3DPConf https://t.co/Ie21Cv0tls

Derek Mathers @MakerMarketer9:53 PM – 20 Oct 2015 to 624 followers 2 0
RT @3DHubs_US: Join #I3DPConf in Santa Clara tomorrow – especially if you’re into #3dprinting and #virtualreality buff.ly/1MyPTHs @I…

Derek Mathers @MakerMarketer9:52 PM – 20 Oct 2015 to 624 followers 3 0
RT @AmericaMakes: Attending #AMmember @Inside3DPrint’s #I3dPConf in Santa Clara? Check out this week’s agenda: bit.ly/1XhSrBw

FREDDY TINAJERO @Optrom_Ecuador9:44 PM – 20 Oct 2015 to 87 followers 9 0

RT @3DPrintingFans: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… http…

tipatat @tipatat8:46 PM – 20 Oct 2015 to 1,692 followers 3 0

RT @onwevr: Also, if you’re at #I3DPConf in Santa Clara on Oct 21, check @auradeluxe’s panel “Will the Future of VR be Mobile?” https://t.c…
Avatarium 3D @Avatarium3D8:37 PM – 20 Oct 2015 to 47 followers 1 2

We’re busy setting up at the 3D Printing Conference & Expo. Stop by booth #303 tomorrow and be wowed! #I3DPConf https://t.co/zFZAYyzVmJ

Wevr @onwevr8:36 PM – 20 Oct 2015 to 1,893 followers 3 2

Also, if you’re at #I3DPConf in Santa Clara on Oct 21, check @auradeluxe’s panel “Will the Future of VR be Mobile?” bit.ly/1XiklgC

ManufacturingStories @MfgStories8:34 PM – 20 Oct 2015 to 11,308 followers 2 0
RT @3DPrint_com: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https:/…

Chris DeCristofaro @cdecrist8:23 PM – 20 Oct 2015 to 116 followers 4 0
RT @3DPrintGirl: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https:/…

alex@fabbing @fabbing8:22 PM – 20 Oct 2015 to 8,565 followers 3 0

RT @brianfederal: Inside 3D Printing #HP displays Sprout #I3DPConf #3dprinting https://t.co/USj7LDeyOg
Brian Federal @brianfederal8:11 PM – 20 Oct 2015 to 10,290 followers 3 4

Inside 3D Printing #HP displays Sprout #I3DPConf #3dprinting https://t.co/USj7LDeyOg

Inside3DPrint @Inside3DPrint7:34 PM – 20 Oct 2015 to 3,143 followers 0 1

.@brianfederal on #multipleintelligences, #mistakes, & #3dprinting. “If you’re not failing, you’re not trying hard enough.” #I3DPConf

Inside3DPrint @Inside3DPrint7:28 PM – 20 Oct 2015 to 3,143 followers 0 1

David Schaefer on why support is necessary in #LMdesign, now @ #I3DPConf #manufacturing tutorial https://t.co/qfUVcuhyFI

Yoni Binstock @YoniBinstock7:16 PM – 20 Oct 2015 to 462 followers 0 1

At #i3dpconf? Then you should check out the top eBook on #3DPrinting #Entrepreneurship – bit.ly/3DEntrepreneur

Inside3DPrint @Inside3DPrint7:00 PM – 20 Oct 2015 to 3,143 followers 0 0

Excited for ‘Direct #Metal #Printing in Applications’ at 2pm? mcklr.me/1VXnTaM @Quickparts @3dsystemscorp #I3DPConf #3dprinting

BONSAI LAB, Inc. @BONSAILAB6:57 PM – 20 Oct 2015 to 649 followers 4 0

RT @formlabs: Headed to 3D Printing Santa Clara today? Don’t forget to stop by booth 502 to see the new Form 2! @Inside3DPrint #I3DPConf #3…

3DPrint @3DPrint_com6:29 PM – 20 Oct 2015 to 33,757 followers 0 0

Off to Santa Clara! Hope to see you all there! #airplanetweet #I3DPConf #livefromI3DPConf

LulzBot @lulzbot3D6:05 PM – 20 Oct 2015 to 2,784 followers 2 0

RT @Inside3DPrint: .@AlephObjects to Release New #Flexystruder Tool Heads for @LulzBot3D Printer Lineup at #I3DPConf Santa Clara https://t.…

Inside3DPrint @Inside3DPrint6:00 PM – 20 Oct 2015 to 3,143 followers 0 2

15 min until tutorial ft @brianfederal, @SandraKayHelsel, @MakerMarketer, & @HoneyPoint3D mcklr.me/1LynUeL #I3DPConf #3dprinting

Andrew Flando @AF_metalformag5:58 PM – 20 Oct 2015 to 98 followers 3 0

RT @AmericaMakes: Attending #AMmember @Inside3DPrint’s #I3dPConf in Santa Clara? Check out this week’s agenda: bit.ly/1XhSrBw

3D Print Week CA @3dprintweekca5:56 PM – 20 Oct 2015 to 81 followers 2 0

RT @Inside3DPrint: .@AlephObjects to Release New #Flexystruder Tool Heads for @LulzBot3D Printer Lineup at #I3DPConf Santa Clara https://t.…

Inside3DPrint @Inside3DPrint5:55 PM – 20 Oct 2015 to 3,143 followers 3 0

RT @3DPrintBoard: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https:…

Inside3DPrint @Inside3DPrint5:55 PM – 20 Oct 2015 to 3,143 followers 2 1

.@AlephObjects to Release New #Flexystruder Tool Heads for @LulzBot3D Printer Lineup at #I3DPConf Santa Clara 3dprint.com/101457/flexyst…

Inside3DPrint @Inside3DPrint5:52 PM – 20 Oct 2015 to 3,143 followers 3 0

RT @brianfederal: #3DPrinting having it’s #Apple moment in Santa Clara @ Inside 3D Printing event #I3DPConf Oct 19th -22nd #3Dprinter https…

Inside3DPrint @Inside3DPrint5:51 PM – 20 Oct 2015 to 3,143 followers 3 0

RT @AmericaMakes: Attending #AMmember @Inside3DPrint’s #I3dPConf in Santa Clara? Check out this week’s agenda: bit.ly/1XhSrBw

America Makes @AmericaMakes5:45 PM – 20 Oct 2015 to 4,938 followers 3 2

Attending #AMmember @Inside3DPrint’s #I3dPConf in Santa Clara? Check out this week’s agenda: bit.ly/1XhSrBw

Michael Makdah @MichaelMakdah5:44 PM – 20 Oct 2015 to 1,234 followers 0 0

Gearing up for #I3DPConf

alex@fabbing @fabbing5:42 PM – 20 Oct 2015 to 8,565 followers 4 0

RT @formlabs: Headed to 3D Printing Santa Clara today? Don’t forget to stop by booth 502 to see the new Form 2! @Inside3DPrint #I3DPConf #3…

Inside3DPrint @Inside3DPrint5:41 PM – 20 Oct 2015 to 3,143 followers 1 0

RT @mbaforyou: The 3rd Industrial Revolution! This Week in Santa Clara @AccountingSuite will join #I3DPConf @Inside3DPrint https://t.co/Gk5…

Inside3DPrint @Inside3DPrint5:30 PM – 20 Oct 2015 to 3,143 followers 4 0

RT @formlabs: Headed to 3D Printing Santa Clara today? Don’t forget to stop by booth 502 to see the new Form 2! @Inside3DPrint #I3DPConf #3…

Formlabs @formlabs5:15 PM – 20 Oct 2015 to 9,648 followers 4 3

Headed to 3D Printing Santa Clara today? Don’t forget to stop by booth 502 to see the new Form 2! @Inside3DPrint #I3DPConf #3dprinting

Inside3DPrint @Inside3DPrint5:05 PM – 20 Oct 2015 to 3,143 followers 0 0

.@brad_rothenberg says #nTopology rolling out pilot programs #I3DPConf #3dprinting

kurt kunselman @mbaforyou5:02 PM – 20 Oct 2015 to 104 followers 1 2
The 3rd Industrial Revolution! This Week in Santa Clara @AccountingSuite will join #I3DPConf @Inside3DPrint ow.ly/TBVz3

AccountingSuite @AccountingSuite5:02 PM – 20 Oct 2015 to 594 followers 0 1

The 3rd Industrial Revolution! This Week in Santa Clara @AccountingSuite will join #I3DPConf @Inside3DPrint ow.ly/TBVCC

Inside3DPrint @Inside3DPrint5:01 PM – 20 Oct 2015 to 3,143 followers 1 0

RT @BunnyEllerin: Thank you!! Excited and on the way to #I3DPConf twitter.com/NYCHBL/status/…

Inside3DPrint @Inside3DPrint5:01 PM – 20 Oct 2015 to 3,143 followers 1 0

RT @GreenfishLabs: Greenfish is coast to coast speaking in a conference in Santa Clara this week and one next week in Philadelphia #MOF #I3…

Rigging Artist @RiggingArtist5:01 PM – 20 Oct 2015 to 486 followers 3 0

RT @Inside3DPrint: “Laser melting, where you see most of the alphabet soup” – @JHornick3D1Stop, @FinneganIPLaw #powderbedfusion #I3DPConf

3DKitbash @3DKitbash5:00 PM – 20 Oct 2015 to 2,745 followers 3 0

RT @Inside3DPrint: “Laser melting, where you see most of the alphabet soup” – @JHornick3D1Stop, @FinneganIPLaw #powderbedfusion #I3DPConf

Inside3DPrint @Inside3DPrint4:58 PM – 20 Oct 2015 to 3,143 followers 1 0

RT @kurthu: It’s Always Sunny in California #I3DPConf https://t.co/j1YV3f0biq

Inside3DPrint @Inside3DPrint4:58 PM – 20 Oct 2015 to 3,143 followers 2 0

RT @brianfederal: Check out #3Dprinting in Education 11:15 today Inside 3D Printing Santa Clara with @brianfederal #I3DPConf #3DPrinters #3…

Greenfish Labs @GreenfishLabs4:56 PM – 20 Oct 2015 to 690 followers 1 1

Greenfish is coast to coast speaking in a conference in Santa Clara this week and one next week in Philadelphia #MOF #I3DPConf

Inside3DPrint @Inside3DPrint4:56 PM – 20 Oct 2015 to 3,143 followers 3 3

“Laser melting, where you see most of the alphabet soup” – @JHornick3D1Stop, @FinneganIPLaw #powderbedfusion #I3DPConf

advnetlab @advnetlab4:55 PM – 20 Oct 2015 to 1,001 followers 4 0

RT @3DPrintingIt: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https:…

o3dprint.ru @o3dprintru4:52 PM – 20 Oct 2015 to 2,600 followers 3 0

RT @brianfederal: #3DPrinting having it’s #Apple moment in Santa Clara @ Inside 3D Printing event #I3DPConf Oct 19th -22nd #3Dprinter https…

alex@fabbing @fabbing4:47 PM – 20 Oct 2015 to 8,565 followers 3 0

RT @brianfederal: #3DPrinting having it’s #Apple moment in Santa Clara @ Inside 3D Printing event #I3DPConf Oct 19th -22nd #3Dprinter https…

Brian Federal @brianfederal4:35 PM – 20 Oct 2015 to 10,290 followers 3 4

#3DPrinting having it’s #Apple moment in Santa Clara @ Inside 3D Printing event #I3DPConf Oct 19th -22nd #3Dprinter https://t.co/quieSTOZCn

Frostclaw20 @frostclaw204:34 PM – 20 Oct 2015 to 158 followers 2 0

RT @3DPrint_com: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https:/…

alex@fabbing @fabbing4:17 PM – 20 Oct 2015 to 8,565 followers 2 0

RT @brianfederal: Check out #3Dprinting in Education 11:15 today Inside 3D Printing Santa Clara with @brianfederal #I3DPConf #3DPrinters #3…

Andrew Flando @AF_metalformag4:06 PM – 20 Oct 2015 to 98 followers 1 0

RT @Inside3DPrint: Let the fun begin! Registration for #I3DPConf Santa Clara is now open! mcklr.me/1PncuwV #3dprinting https://t.co/…

NYC Health Business @NYCHBL4:04 PM – 20 Oct 2015 to 1,655 followers 0 1
@NYCHBL Pres. @BunnyEllerin is medical track chair for Inside 3D Print Expo ow.ly/TuBr9 @3DPrint_com #3dprinting #I3DPConf

Brian Federal @brianfederal4:03 PM – 20 Oct 2015 to 10,290 followers 2 1

Check out #3Dprinting in Education 11:15 today Inside 3D Printing Santa Clara with @brianfederal #I3DPConf #3DPrinters #3Dprint #HP

Inside3DPrint @Inside3DPrint4:00 PM – 20 Oct 2015 to 3,143 followers 0 1

#I3DPConf Santa Clara tutorial on #GenerativeMicrostructures w/ @brad_rothenberg starts NOW mcklr.me/1VSgnup #3dprinting

Kurt Hunter @kurthu3:54 PM – 20 Oct 2015 to 62 followers 1 3

It’s Always Sunny in California #I3DPConf https://t.co/j1YV3f0biq

Brian Federal @brianfederal3:54 PM – 20 Oct 2015 to 10,290 followers 4 0

RT @Inside3DPrint: THANK YOU #I3DPConf Zhuhai speakers, attendees, & sponsors. What a great show! bit.ly/1N3wL7s #3dprinting @Recy…

Brian Federal @brianfederal3:50 PM – 20 Oct 2015 to 10,290 followers 2 0

RT @My3DPrinting: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https:…

Margaret @hatzbymm3:31 PM – 20 Oct 2015 to 1,095 followers 4 0

RT @3DPrintingIt: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https:…

डॉ हर्षित दवे @hkdave3:20 PM – 20 Oct 2015 to 261 followers 9 0
RT @3DPrintingFans: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… http…

Inside3DPrint @Inside3DPrint3:15 PM – 20 Oct 2015 to 3,143 followers 1 1

Let the fun begin! Registration for #I3DPConf Santa Clara is now open! mcklr.me/1PncuwV #3dprinting https://t.co/ra2r4fmLgA

Diego Camilo Peña R @nervencid2:52 PM – 20 Oct 2015 to 303 followers 9 0
RT @3DPrintingFans: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… http…

be connect3D @beconnect3D2:49 PM – 20 Oct 2015 to 1,338 followers 9 0
RT @3DPrintingFans: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… http…

Vinay Shenoy @vinayshenoy12:48 PM – 20 Oct 2015 to 22 followers 2 0

RT @3D_Print_News: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https…

Bazooka Tech @bazooka_tech2:44 PM – 20 Oct 2015 to 1,173 followers 4 0
RT @3DPrintingIt: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https:…

alex@fabbing @fabbing2:34 PM – 20 Oct 2015 to 8,565 followers 3 0

RT @3DPrintBoard: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https:…

カモノハシ @roso19542:32 PM – 20 Oct 2015 to 9,915 followers 0 0
＠3DPrintGirl: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf … https://t.co/3uoVVH2TNH

EDGARDOGARCIASALGADO @EDGARDOGARCIASA2:31 PM – 20 Oct 2015 to 19,142 followers 9 0

RT @3DPrintingFans: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… http…

そむにうむ＠森山弘樹 βテスター募集中 @Somnium2:31 PM – 20 Oct 2015 to 4,049 followers 9 0
RT @3DPrintingFans: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… http…

3D Printing News @My3DPrinting2:30 PM – 20 Oct 2015 to 54,848 followers 2 4

Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https://t.co/i6cYNZNjYO

3D Printing @3DPrintBoardcom2:30 PM – 20 Oct 2015 to 11,478 followers 0 1

Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https://t.co/dE4VUBIx7V

3D Printing Fans @3DPrintingFans2:30 PM – 20 Oct 2015 to 17,194 followers 9 4

Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https://t.co/V4ubYDvvRK

3D Print .com @3DPrintBoard2:30 PM – 20 Oct 2015 to 46,499 followers 3 1

Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https://t.co/0phMdTAtav

3D Printing @3DPrintingIt2:30 PM – 20 Oct 2015 to 15,317 followers 4 6

Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https://t.co/4XkrC3FT9U

Ra @freakain2:21 PM – 20 Oct 2015 to 152 followers 4 0

RT @3DPrintGirl: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https:/…

3D Print Week CA @3dprintweekca2:19 PM – 20 Oct 2015 to 81 followers 1 0

RT @Inside3DPrint: Excited for tutorials today? Registration opens at 8:15! #I3DPConf https://t.co/l2XzHvp685
Bunny Ellerin @BunnyEllerin2:19 PM – 20 Oct 2015 to 2,450 followers 1 3

Thank you!! Excited and on the way to #I3DPConf twitter.com/NYCHBL/status/…

Inside3DPrint @Inside3DPrint2:18 PM – 20 Oct 2015 to 3,143 followers 1 0

Excited for tutorials today? Registration opens at 8:15! #I3DPConf https://t.co/l2XzHvp685

HomeZilla @homezillaorguk2:17 PM – 20 Oct 2015 to 821 followers 2 0

RT @3D_Print_News: Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https…

3D Printing @3D_Printing_it2:10 PM – 20 Oct 2015 to 2,209 followers 0 1

Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https://t.co/tN6Tsw1EOh

3D Printing News @3DNewsNow2:10 PM – 20 Oct 2015 to 3,314 followers 1 1
Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https://t.co/URNocCGYYT

3D Printing @3D_Print_News2:10 PM – 20 Oct 2015 to 12,523 followers 2 0

Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https://t.co/2j9ZQ1I1d9

3D Printing News @3DPrintGirl2:10 PM – 20 Oct 2015 to 40,617 followers 4 4

Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https://t.co/KtHqxkKDcI

3DPrint @3DPrint_com2:10 PM – 20 Oct 2015 to 33,757 followers 2 1

Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https://t.co/vY1L1xhRNU

BayAreaStartupEvents @BayAreaStartup1:50 PM – 20 Oct 2015 to 609 followers 1 0

RT @3DPrint_com: We’re getting ready for #I3DPConf in Santa Clara! Stay tuned here for live tweets throughout the event on the 21st & 22nd!
Inside3DPrint @Inside3DPrint1:46 PM – 20 Oct 2015 to 3,143 followers 8 0

RT @DesignBox3D: We’re at #I3DPConf in #SantaClara this week with our friends @ZMorph3d 😎 Stop by and see us #3DPrinting #Fabrication https…

3DPrint @3DPrint_com1:41 PM – 20 Oct 2015 to 33,757 followers 1 1

We’re getting ready for #I3DPConf in Santa Clara! Stay tuned here for live tweets throughout the event on the 21st & 22nd!
3-D Print Factory @3_dprintfactory1:08 PM – 20 Oct 2015 to 57 followers 0 0

Come visit @3_dprintfactory at booth 303, where we are debuting the @Avatarium3D turnkey modular array. #I3DPConf twitter.com/Avatarium3D/st…

Bazooka Tech @bazooka_tech1:06 PM – 20 Oct 2015 to 1,173 followers 2 0
RT @brianfederal: Hod Lipson will be presenting today at Inside 3D Printing Santa Clara @ 4:15. #I3DPConf #3dprinting

Inside3DPrint @Inside3DPrint1:00 PM – 20 Oct 2015 to 3,143 followers 0 0

CONFIRMED: @esun3dfilament will exhibit at #I3DPConf Mumbai mcklr.me/1kltmrc #3dprinting

alex@fabbing @fabbing12:44 PM – 20 Oct 2015 to 8,565 followers 2 0

RT @brianfederal: Hod Lipson will be presenting today at Inside 3D Printing Santa Clara @ 4:15. #I3DPConf #3dprinting

Brian Federal @brianfederal12:18 PM – 20 Oct 2015 to 10,290 followers 2 2

Hod Lipson will be presenting today at Inside 3D Printing Santa Clara @ 4:15. #I3DPConf #3dprinting

Wallapa. @Wallapatuk7:36 AM – 20 Oct 2015 to 530 followers 4 0

RT @My3DPrinting: Details on Laurence Gartel’s Creative Force Design Competition at #I3DPConf in Santa Clara! 3dprint.com/99105/gartel-c… http:/…

Laurence Gartel @GARTEL7:36 AM – 20 Oct 2015 to 558 followers 2 0

RT @3DPrintBoard: For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #gart…

Laurence Gartel @GARTEL7:36 AM – 20 Oct 2015 to 558 followers 3 0

RT @3DPrintGirl: For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #garte…

江川法人 @egawa_norito4:53 AM – 20 Oct 2015 to 580 followers 8 0

RT @DesignBox3D: We’re at #I3DPConf in #SantaClara this week with our friends @ZMorph3d 😎 Stop by and see us #3DPrinting #Fabrication https…

3D Printing @Just_Print_It4:43 AM – 20 Oct 2015 to 839 followers 8 0

RT @DesignBox3D: We’re at #I3DPConf in #SantaClara this week with our friends @ZMorph3d 😎 Stop by and see us #3DPrinting #Fabrication https…

Rapid Prototyping @rapidprototypin4:23 AM – 20 Oct 2015 to 3,396 followers 8 0

RT @DesignBox3D: We’re at #I3DPConf in #SantaClara this week with our friends @ZMorph3d 😎 Stop by and see us #3DPrinting #Fabrication https…

Allandnnn @allandnnn4:19 AM – 20 Oct 2015 to 783 followers 8 0

RT @DesignBox3D: We’re at #I3DPConf in #SantaClara this week with our friends @ZMorph3d 😎 Stop by and see us #3DPrinting #Fabrication https…

Preet Jesrani @PreetJesrani4:17 AM – 20 Oct 2015 to 196 followers 8 0

RT @DesignBox3D: We’re at #I3DPConf in #SantaClara this week with our friends @ZMorph3d 😎 Stop by and see us #3DPrinting #Fabrication https…

DB3D Distribution @DB3Distribution4:16 AM – 20 Oct 2015 to 408 followers 8 0

RT @DesignBox3D: We’re at #I3DPConf in #SantaClara this week with our friends @ZMorph3d 😎 Stop by and see us #3DPrinting #Fabrication https…

DesignBox3D @DesignBox3D4:16 AM – 20 Oct 2015 to 2,994 followers 8 9

We’re at #I3DPConf in #SantaClara this week with our friends @ZMorph3d 😎 Stop by and see us #3DPrinting #Fabrication https://t.co/E1hLKqvr6z

Computer Print News @Recycling_Times3:11 AM – 20 Oct 2015 to 15,000 followers 0 1

Missed the #I3DPConf in Zhuhai? Don’t worry, you will get your next chance in #Shanghai this December! twitter.com/Inside3DPrint/…

FREDDY TINAJERO @Optrom_Ecuador3:06 AM – 20 Oct 2015 to 87 followers 1 0

RT @3D_Print_News: For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #gar…

connie874cs@gmail.co @connie874cs1:27 AM – 20 Oct 2015 to 146 followers 3 0

RT @3DPrintGirl: For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #garte…

Bazooka Tech @bazooka_tech11:37 PM – 19 Oct 2015 to 1,173 followers 2 0
RT @brianfederal: Yosemite from the air on trip to Santa Clara today for Inside 3D Printing. #I3DPConf #3dprinting https://t.co/TDdUmHK3QQ

Inside3DPrint @Inside3DPrint11:30 PM – 19 Oct 2015 to 3,143 followers 1 0

RT @Forecast3D: In 2 days you can find us at #I3DPConf in Santa Clara! Tomorrow is the last day for FREE registration: http://t.co/xnfRt4sI…
Inside3DPrint @Inside3DPrint11:29 PM – 19 Oct 2015 to 3,143 followers 1 0

RT @3_dprintfactory: We’ll be in Santa Clara tomorrow! Visit us to learn more about the Avatarium (TM), the most advanced 3-D Capture array…
Inside3DPrint @Inside3DPrint11:29 PM – 19 Oct 2015 to 3,143 followers 1 0

RT @brianfederal: Join me at Inside 3D Printing Conference Santa Clara Tomorrow October 20th 11:15 3DP in Education #3DPrinting #I3DPConf …

Inside3DPrint @Inside3DPrint11:28 PM – 19 Oct 2015 to 3,143 followers 2 0

RT @SprintRay: In the Bay Area? Come visit our booth at @Inside3DPrint in Santa Clara this Wed & Thurs! #I3DPConf http://t.co/OMrRKhTGBr

Inside3DPrint @Inside3DPrint11:28 PM – 19 Oct 2015 to 3,143 followers 2 0

RT @AmericaMakes: For #3DPrinting & #virtualreality fanatics, join @Inside3DPrint at the #I3DPConf tmrw in Santa Clara! https://t.co/56C8OG…

Inside3DPrint @Inside3DPrint11:28 PM – 19 Oct 2015 to 3,143 followers 0 0

We’re excited to see you again! #I3DPConf twitter.com/Raise3D/status…

alex@fabbing @fabbing10:58 PM – 19 Oct 2015 to 8,565 followers 2 0

RT @brianfederal: Yosemite from the air on trip to Santa Clara today for Inside 3D Printing. #I3DPConf #3dprinting https://t.co/TDdUmHK3QQ

katia a @klaka8510:54 PM – 19 Oct 2015 to 477 followers 3 0
RT @3DPrintGirl: For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #garte…

Brian Federal @brianfederal10:48 PM – 19 Oct 2015 to 10,290 followers 2 2

Yosemite from the air on trip to Santa Clara today for Inside 3D Printing. #I3DPConf #3dprinting https://t.co/TDdUmHK3QQ

Brian Federal @brianfederal10:43 PM – 19 Oct 2015 to 10,290 followers 1 0

Join me at Inside 3D Printing Conference Santa Clara Tomorrow October 20th 11:15 3DP in Education #3DPrinting #I3DPConf #HP #HPSPROUT

Rapid Prototyping @rapidprototypin9:18 PM – 19 Oct 2015 to 3,396 followers 2 0

RT @AmericaMakes: For #3DPrinting & #virtualreality fanatics, join @Inside3DPrint at the #I3DPConf tmrw in Santa Clara! https://t.co/56C8OG…

3-D Print Factory @3_dprintfactory9:00 PM – 19 Oct 2015 to 57 followers 0 1

3-D @3_dprintfactory will be with the folks from Avatarium 3D in Santa Clara. Come by & see us! #I3DPConf twitter.com/Avatarium3D/st…

3-D Print Factory @3_dprintfactory8:59 PM – 19 Oct 2015 to 57 followers 1 1

We’ll be in Santa Clara tomorrow! Visit us to learn more about the Avatarium (TM), the most advanced 3-D Capture array in the biz. #I3DPConf

America Makes @AmericaMakes8:45 PM – 19 Oct 2015 to 4,938 followers 2 2

For #3DPrinting & #virtualreality fanatics, join @Inside3DPrint at the #I3DPConf tmrw in Santa Clara! bit.ly/1Xf8joi #3DPweek

christine trageser @christinatee8:37 PM – 19 Oct 2015 to 203 followers 1 0

RT @Inside3DPrint: Drop by #I3DPConf Booth #221 next week for launch of #3DMetalPrinting Magazine mcklr.me/1hJjcyw @PMATalk @Meckle…

3D Print Week CA @3dprintweekca8:30 PM – 19 Oct 2015 to 81 followers 0 0

Drop by #I3DPConf Booth #221 this week for launch of #3DMetalPrinting Magazine mcklr.me/1hJjcyw @PMATalk @MecklerMedia #3dprinting

MecklerMedia @MecklerMedia8:30 PM – 19 Oct 2015 to 305 followers 0 0

Drop by #I3DPConf Booth #221 this week for launch of #3DMetalPrinting Magazine mcklr.me/1hJjcyw @PMATalk #3dprinting

Inside3DPrint @Inside3DPrint8:30 PM – 19 Oct 2015 to 3,143 followers 1 1

Drop by #I3DPConf Booth #221 next week for launch of #3DMetalPrinting Magazine mcklr.me/1hJjcyw @PMATalk @MecklerMedia #3dprinting

Forecast3D @Forecast3D5:55 PM – 19 Oct 2015 to 172 followers 1 3
In 2 days you can find us at #I3DPConf in Santa Clara! Tomorrow is the last day for FREE registration: inside3dprinting.com/santa-clara/20… #3dprinting

カモノハシ @roso19545:08 PM – 19 Oct 2015 to 9,915 followers 0 1
＠3DPrintGirl: For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #gartel…

alex@fabbing @fabbing5:04 PM – 19 Oct 2015 to 8,565 followers 2 0

RT @3DPrintBoard: For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #gart…

MecklerMedia @MecklerMedia5:00 PM – 19 Oct 2015 to 305 followers 0 0

Save $ by registering online for #I3DPConf Santa Clara. Prices go up onsite bit.ly/1PhkAqJ #3dprinting http://t.co/TZ0BuQTgpu

Inside3DPrint @Inside3DPrint5:00 PM – 19 Oct 2015 to 3,143 followers 0 0

Save $ by registering online for #I3DPConf Santa Clara. Prices go up onsite bit.ly/1PhkAqJ #3dprinting http://t.co/DnfIDNL3Au

3D Printing News @3DNewsNow5:00 PM – 19 Oct 2015 to 3,314 followers 0 1
For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #gartel3d @GARTEL

3D Printing @3D_Print_News5:00 PM – 19 Oct 2015 to 12,523 followers 1 3

For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #gartel3d @GARTEL

3D Print .com @3DPrintBoard5:00 PM – 19 Oct 2015 to 46,499 followers 2 4

For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #gartel3d @GARTEL

3DPrint @3DPrint_com5:00 PM – 19 Oct 2015 to 33,757 followers 0 1

For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #gartel3d @GARTEL

3D Printing News @3DPrintGirl5:00 PM – 19 Oct 2015 to 40,617 followers 3 3

For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #gartel3d @GARTEL

3D Printing @3DPrintingIt5:00 PM – 19 Oct 2015 to 15,317 followers 0 1

For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #gartel3d @GARTEL

3D Printing @3D_Printing_it5:00 PM – 19 Oct 2015 to 2,209 followers 0 1

For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #gartel3d @GARTEL

3D Printing News @My3DPrinting5:00 PM – 19 Oct 2015 to 54,848 followers 0 1

For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #gartel3d @GARTEL

3D Printing @3DPrintBoardcom5:00 PM – 19 Oct 2015 to 11,478 followers 0 1

For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #gartel3d @GARTEL

3D Printing Fans @3DPrintingFans5:00 PM – 19 Oct 2015 to 17,194 followers 0 1

For the 1st time @ #I3DPConf, #CreativeForce #Design Competition in CA this week bit.ly/1Oag9xS #3dprinting #gartel3d @GARTEL

SprintRay @SprintRay4:28 PM – 19 Oct 2015 to 347 followers 2 2

In the Bay Area? Come visit our booth at @Inside3DPrint in Santa Clara this Wed & Thurs! #I3DPConf http://t.co/OMrRKhTGBr

HiTechAgenda @HiTechAgenda4:05 PM – 19 Oct 2015 to 2,294 followers 0 1

The Inside 3D Printing Santa Clara, October 20-22, USA. Learn more: ow.ly/SssSI @Inside3DPrint #I3DPConf http://t.co/4Ty3hkdDT8

NYC Health Business @NYCHBL4:04 PM – 19 Oct 2015 to 1,655 followers 0 0
2-day medical track set for 3D print expo, feat. @NYCHBL pres. @BunnyEllerin Starts tomrw: ow.ly/TuAjq @3DPrint_com #I3DPConf

Inside3DPrint @Inside3DPrint2:28 PM – 19 Oct 2015 to 3,143 followers 2 0

RT @3DHubs_US: Join #I3DPConf in Santa Clara tomorrow – especially if you’re into #3dprinting and #virtualreality buff.ly/1MyPTHs @I…

3D Hubs USA @3DHubs_US2:21 PM – 19 Oct 2015 to 2,072 followers 2 5

Join #I3DPConf in Santa Clara tomorrow – especially if you’re into #3dprinting and #virtualreality buff.ly/1MyPTHs @Inside3DPrint

Inside3DPrint @Inside3DPrint4:48 AM – 19 Oct 2015 to 3,143 followers 2 0

RT @TheCSUSA: Coming up: #CSUSAmember John Hornick on “3D Printing and the Future (or Demise) of IP” ow.ly/TvDRe at #I3DPConf

Inside3DPrint @Inside3DPrint4:48 AM – 19 Oct 2015 to 3,143 followers 1 0

RT @ElliotKotek: It’s #3dprinting week in California! Join our conversation @ #I3DPConf (Inside 3D Printing Conference) in Santa Clara on W…
Inside3DPrint @Inside3DPrint4:46 AM – 19 Oct 2015 to 3,143 followers 1 0

RT @MakePrintable: Come visit our booth in @Inside3DPrint Sanata Clara and join our beta hero program. #I3DPConf http://t.co/EdahS2h8OE

MakePrintable @MakePrintable12:15 AM – 19 Oct 2015 to 31 followers 1 2

Come visit our booth in @Inside3DPrint Sanata Clara and join our beta hero program. #I3DPConf http://t.co/EdahS2h8OE

John Meckler @johnmeckler11:35 PM – 18 Oct 2015 to 325 followers 1 0

RT @Inside3DPrint: #blendedreality takes center stage at #I3DPConf Santa Clara w/ the #scanners & #software track pass http://t.co/GYyQXIsb…

Elliot Kotek @ElliotKotek9:07 PM – 18 Oct 2015 to 1,316 followers 1 3

It’s #3dprinting week in California! Join our conversation @ #I3DPConf (Inside 3D Printing Conference) in Santa Clara on Wednesday 10/21!
Matthew Sag @matthewsag7:12 PM – 18 Oct 2015 to 860 followers 2 0

RT @TheCSUSA: Coming up: #CSUSAmember John Hornick on “3D Printing and the Future (or Demise) of IP” ow.ly/TvDRe at #I3DPConf

Inside3DPrint @Inside3DPrint7:00 PM – 18 Oct 2015 to 3,143 followers 1 0

#blendedreality takes center stage at #I3DPConf Santa Clara w/ the #scanners & #software track pass bit.ly/1hgqFFd #3dprinting

3DPrintingSolutions @3DPrintingTexas6:37 PM – 18 Oct 2015 to 26 followers 6 0

RT @BunnyEllerin: Inspiring & Relevant: 2 day Medical Track Set for Inside 3D Printing Conference next week in Santa Clara #i3dpconf http:/…

3DPrintingSolutions @3DPrintingTexas6:37 PM – 18 Oct 2015 to 26 followers 4 0

RT @3DPrintGirl: “Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.c…

Invest Zhuhai @investzhuhai6:23 PM – 18 Oct 2015 to 760 followers 4 0

RT @Inside3DPrint: THANK YOU #I3DPConf Zhuhai speakers, attendees, & sponsors. What a great show! bit.ly/1N3wL7s #3dprinting @Recy…

TechSharer @Tech_Sharer6:15 PM – 18 Oct 2015 to 5,368 followers 4 0
RT @Inside3DPrint: #3dprinting industry experts answer your burning questions @ @TheUPSStore panel, #I3DPConf mcklr.me/1LmSvbw http:…

Bazooka Tech @bazooka_tech6:14 PM – 18 Oct 2015 to 1,173 followers 4 0
RT @Inside3DPrint: #3dprinting industry experts answer your burning questions @ @TheUPSStore panel, #I3DPConf mcklr.me/1LmSvbw http:…

Copyright Society @TheCSUSA6:02 PM – 18 Oct 2015 to 2,851 followers 2 1
Coming up: #CSUSAmember John Hornick on “3D Printing and the Future (or Demise) of IP” ow.ly/TvDRe at #I3DPConf

Inside3DPrint @Inside3DPrint6:00 PM – 18 Oct 2015 to 3,143 followers 0 0

Register online for #I3DPConf Santa Clara to save. Onsite prices are higher bit.ly/1PhkAqJ #3dprinting http://t.co/WULqmDYWoJ

Tech XB @TechXB5:51 PM – 18 Oct 2015 to 3,394 followers 4 0

RT @Inside3DPrint: #3dprinting industry experts answer your burning questions @ @TheUPSStore panel, #I3DPConf mcklr.me/1LmSvbw http:…

The UPS Store @TheUPSStore5:40 PM – 18 Oct 2015 to 11,688 followers 4 0
RT @Inside3DPrint: #3dprinting industry experts answer your burning questions @ @TheUPSStore panel, #I3DPConf mcklr.me/1LmSvbw http:…

Faisal Nimri @NIMRI_905:05 PM – 18 Oct 2015 to 589 followers 2 0
RT @MakePrintable: Come visit our booth in @Inside3DPrint Sanata Clara and join our beta hero program. #I3DPConf #3dprinting #3D http://t.…

Inside3DPrint @Inside3DPrint5:00 PM – 18 Oct 2015 to 3,143 followers 4 3

#3dprinting industry experts answer your burning questions @ @TheUPSStore panel, #I3DPConf mcklr.me/1LmSvbw http://t.co/EUe1FIq9FX

Bazooka Tech @bazooka_tech4:50 PM – 18 Oct 2015 to 1,173 followers 2 0
RT @MakePrintable: Come visit our booth in @Inside3DPrint Sanata Clara and join our beta hero program. #I3DPConf #3dprinting #3D http://t.…

MakePrintable @MakePrintable4:15 PM – 18 Oct 2015 to 31 followers 2 1

Come visit our booth in @Inside3DPrint Sanata Clara and join our beta hero program. #I3DPConf #3dprinting #3D http://t.co/lEzJxNjIOz

Inkjet Forum India @inkjet_india7:17 AM – 18 Oct 2015 to 985 followers 4 0

RT @Inside3DPrint: THANK YOU #I3DPConf Zhuhai speakers, attendees, & sponsors. What a great show! bit.ly/1N3wL7s #3dprinting @Recy…

Inkjet Forum India @inkjet_india7:17 AM – 18 Oct 2015 to 985 followers 1 0

RT @Inside3DPrint: Don’t miss @TerryWohlers in Shanghai at this December’s #I3DPConf bit.ly/1jwqaIy #3dprinting http://t.co/JA0Mm9L…

IRiS 3D Solutions @iris3dsolutions5:25 AM – 18 Oct 2015 to 4,649 followers 3 0

RT @My3DPrinting: “Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.…

3D Print Week CA @3dprintweekca2:12 AM – 18 Oct 2015 to 81 followers 1 0

RT @Inside3DPrint: .@TheUPSStore’s “Ask the Experts” Panel at #I3DPConf Will Answer All Your #3DPrinting Questions bit.ly/1hJuPWe (…

3D Print Week CA @3dprintweekca2:12 AM – 18 Oct 2015 to 81 followers 6 0

RT @BunnyEllerin: Inspiring & Relevant: 2 day Medical Track Set for Inside 3D Printing Conference next week in Santa Clara #i3dpconf http:/…

Inside3DPrint @Inside3DPrint2:09 AM – 18 Oct 2015 to 3,143 followers 1 0

RT @Recycling_Times: Steve Ma from #Xuberance talking about #3Dprinted design for weddings at #I3DPConf at #RWE2015 http://t.co/iUrkuTciJo

Inside3DPrint @Inside3DPrint2:09 AM – 18 Oct 2015 to 3,143 followers 6 0

RT @BunnyEllerin: Inspiring & Relevant: 2 day Medical Track Set for Inside 3D Printing Conference next week in Santa Clara #i3dpconf http:/…

Inside3DPrint @Inside3DPrint2:08 AM – 18 Oct 2015 to 3,143 followers 1 0

RT @GARTEL: #I3DPConf Santa Clara your #creativity w/ #CreativeForce #Design Competition bit.ly/1Oag9xS #3dprinting http://t.co/qWr…

Inside3DPrint @Inside3DPrint7:00 PM – 17 Oct 2015 to 3,143 followers 0 0

#I3DPConf Santa Clara Networking Pass goes from FREE to $20 onsite, so register early! bit.ly/1PhkAqJ #3dprinting

Inside3DPrint @Inside3DPrint5:00 PM – 17 Oct 2015 to 3,143 followers 1 2

Don’t miss @TerryWohlers in Shanghai at this December’s #I3DPConf bit.ly/1jwqaIy #3dprinting http://t.co/JA0Mm9L7Bv

Hackdaddy @TheHackdaddy2:59 PM – 17 Oct 2015 to 760 followers 4 0

RT @3DPrintGirl: “Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.c…

Bazooka Tech @bazooka_tech10:33 AM – 17 Oct 2015 to 1,173 followers 4 0
RT @Inside3DPrint: THANK YOU #I3DPConf Zhuhai speakers, attendees, & sponsors. What a great show! bit.ly/1N3wL7s #3dprinting @Recy…

Inside3DPrint @Inside3DPrint10:00 AM – 17 Oct 2015 to 3,143 followers 4 4

THANK YOU #I3DPConf Zhuhai speakers, attendees, & sponsors. What a great show! bit.ly/1N3wL7s #3dprinting @Recycling_Times

Inside3DPrint @Inside3DPrint5:00 AM – 17 Oct 2015 to 3,143 followers 0 1

The #I3DPConf Zhuhai expo hall closes in a few hours! Visit all of your favorite booths bit.ly/1jTu92n #3dprinting @Recycling_Times

Inside3DPrint @Inside3DPrint10:23 PM – 16 Oct 2015 to 3,143 followers 1 0

.@TheUPSStore’s “Ask the Experts” Panel at #I3DPConf Will Answer All Your #3DPrinting Questions bit.ly/1hJuPWe (via @3DPrint_com)

Inside3DPrint @Inside3DPrint10:00 PM – 16 Oct 2015 to 3,143 followers 0 1

If you don’t want 2 pay more @ the door, register online! #I3DPConf Santa Clara bit.ly/1PhkAqJ #3dprinting http://t.co/1bb4BT3lRd

Shield R & D @research_shield9:07 PM – 16 Oct 2015 to 30 followers 0 1

“Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.co/ETJUkp3sYt

John Pennett @j_pennett8:21 PM – 16 Oct 2015 to 1,160 followers 6 0
RT @BunnyEllerin: Inspiring & Relevant: 2 day Medical Track Set for Inside 3D Printing Conference next week in Santa Clara #i3dpconf http:/…

Bazooka Tech @bazooka_tech8:11 PM – 16 Oct 2015 to 1,173 followers 3 0
RT @My3DPrinting: “Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.…

sharedvision @sharedvision_eu7:48 PM – 16 Oct 2015 to 128 followers 3 0

RT @My3DPrinting: “Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.…

alex@fabbing @fabbing7:26 PM – 16 Oct 2015 to 8,565 followers 1 0

RT @3DPrintBoard: “Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.…

3DPrint @3DPrint_com7:25 PM – 16 Oct 2015 to 33,757 followers 0 0

“Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.co/2icCVoX4ls

3D Printing News @3DNewsNow7:25 PM – 16 Oct 2015 to 3,314 followers 0 0
“Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.co/jfNBIvrE75

3D Printing News @My3DPrinting7:25 PM – 16 Oct 2015 to 54,848 followers 3 0

“Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.co/y9TsoBtMKd

3D Print .com @3DPrintBoard7:25 PM – 16 Oct 2015 to 46,499 followers 1 0

“Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.co/JwvyrY3t3v

3D Printing @3D_Printing_it7:25 PM – 16 Oct 2015 to 2,209 followers 0 0

“Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.co/BqyBcT0QeG

3D Printer News @3DPrinterNews7:20 PM – 16 Oct 2015 to 1,078 followers 0 0

twitter.com/3DPrintBoardco… “Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions … http://t.co/Wwn9lHcmFH

Laurence Gartel @GARTEL7:18 PM – 16 Oct 2015 to 558 followers 1 2

#I3DPConf Santa Clara your #creativity w/ #CreativeForce #Design Competition bit.ly/1Oag9xS #3dprinting http://t.co/qWr5q5iVu9

Bazooka Tech @bazooka_tech7:17 PM – 16 Oct 2015 to 1,173 followers 2 0
RT @3DPrintingIt: “Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.…

カモノハシ @roso19547:16 PM – 16 Oct 2015 to 9,915 followers 0 0
＠3DPrintGirl: “Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions … http://t.co/R6O5WNk51e

Sabine Slaughter @SASlaugh7:11 PM – 16 Oct 2015 to 312 followers 1 0
RT @3DPrintBoardcom: “Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http:/…

1874AntBarberia @1874AntBarberia7:09 PM – 16 Oct 2015 to 514 followers 1 0

RT @3D_Print_News: “Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t…

HomeZilla @homezillaorguk7:08 PM – 16 Oct 2015 to 821 followers 2 0

RT @3DPrintingIt: “Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.…

Michael Mangold @mikerdot7:07 PM – 16 Oct 2015 to 465 followers 4 0

RT @3DPrintGirl: “Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.c…

3D Printing @3DPrintingIt7:07 PM – 16 Oct 2015 to 15,317 followers 2 0

“Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.co/1ji0zQIdj6

3D Printing Fans @3DPrintingFans7:07 PM – 16 Oct 2015 to 17,194 followers 0 0

“Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.co/cGyrqiUEHn

3D Printing @3D_Print_News7:07 PM – 16 Oct 2015 to 12,523 followers 1 0

“Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.co/CruVu3K5CV

3D Printing @3DPrintBoardcom7:07 PM – 16 Oct 2015 to 11,478 followers 1 0

“Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.co/5MpE6YxnTG

3D Printing News @3DPrintGirl7:07 PM – 16 Oct 2015 to 40,617 followers 4 2

“Ask the Experts” Panel at #I3DPConf Will Answer All Your Pressing #3DPrinting Questions 3dprint.com/100610/ups-sto… http://t.co/M6dkNOwzj5

Inside3DPrint @Inside3DPrint7:00 PM – 16 Oct 2015 to 3,143 followers 0 0

Learn how to #market & #brand your #3dprinting #startup w/ #I3DPConf Santa Clara’s #business track pass bit.ly/1hgqFFd

Scansite @Scansite3D6:33 PM – 16 Oct 2015 to 13 followers 1 0
RT @Inside3DPrint: CONFIRMED: @Scansite3D to exhibit at #I3DPConf Santa Clara mcklr.me/1OyWYOl #3dprinting http://t.co/lVlsmm8TZR

Inside3DPrint @Inside3DPrint6:30 PM – 16 Oct 2015 to 3,143 followers 1 1

CONFIRMED: @Scansite3D to exhibit at #I3DPConf Santa Clara mcklr.me/1OyWYOl #3dprinting http://t.co/lVlsmm8TZR

Inside3DPrint @Inside3DPrint5:00 PM – 16 Oct 2015 to 3,143 followers 0 0

See @Airavata3D at #I3DPConf Mumbai! airavata.co #3dprinting http://t.co/ovc2UXDq9d

NYC Health Business @NYCHBL4:54 PM – 16 Oct 2015 to 1,655 followers 6 0
RT @BunnyEllerin: Inspiring & Relevant: 2 day Medical Track Set for Inside 3D Printing Conference next week in Santa Clara #i3dpconf http:/…

BayAreaStartupEvents @BayAreaStartup4:17 PM – 16 Oct 2015 to 609 followers 6 0

RT @BunnyEllerin: Inspiring & Relevant: 2 day Medical Track Set for Inside 3D Printing Conference next week in Santa Clara #i3dpconf http:/…

Bunny Ellerin @BunnyEllerin4:10 PM – 16 Oct 2015 to 2,450 followers 6 4

Inspiring & Relevant: 2 day Medical Track Set for Inside 3D Printing Conference next week in Santa Clara #i3dpconf ow.ly/TuvnR

Inside3DPrint @Inside3DPrint2:00 PM – 16 Oct 2015 to 3,143 followers 0 0

.@Raise3D launches @Kickstarter for #3dprinter line. #I3DPConf exhibitor kck.st/1k7HHHq #3dprinting http://t.co/7hiLpqKQnP

Eddie Krassenstein @EddieKrassenste1:55 PM – 16 Oct 2015 to 2,933 followers 2 0

RT @3DPrint_com: The Medical Track offers incredible sessions #3Dprinting sessions at #I3DPConf next week! 3dprint.com/100840/medical… http://t.…

Mindsparkz @mindsparkz10:13 AM – 16 Oct 2015 to 195 followers 0 1

Thank you @Inside3DPrint Had an amazing day at #I3DPConf #3D #designlife #innovation #edtech

Computer Print News @Recycling_Times6:24 AM – 16 Oct 2015 to 15,000 followers 1 1

Steve Ma from #Xuberance talking about #3Dprinted design for weddings at #I3DPConf at #RWE2015 http://t.co/iUrkuTciJo

Imaginarium India @Imaginarium_Ind4:42 AM – 16 Oct 2015 to 81 followers 1 0

RT @Inside3DPrint: #I3DPConf Mumbai Keynote #business track: @ImaginariumRao of @Imaginarium_Ind bit.ly/1VR2hYr #3dprinting http://…

Inside3DPrint @Inside3DPrint4:10 AM – 16 Oct 2015 to 3,143 followers 0 1

After lunch, check out ‘#3DDesign: To Unveil an Era of Customization’ #I3DPConf Zhuhai bit.ly/1N3QlAc #3dprinting @Recycling_Times

Older tweets mentioning #I3DPConf are not available
Most retweeted tweets
3D Printing News @3DPrintGirl9:05 PM – 22 Oct 2015 via TweetDeck 15 7

‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” 3dprint.com/102036/3ds-key… #I3DPConf https://t.co/HVwyox6JAj

3D Printing News @3DPrintGirl2:38 PM – 23 Oct 2015 via TweetDeck 10 12

Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/WPhiwzVZjN

la moustache ringard @SJGrunewald5:42 PM – 21 Oct 2015 via Twitter for iPhone 10 9

@GoEngineer 3D printed #halo figure. One piece on #objetconnex @Stratasys multi color and materials. #I3DPConf https://t.co/7Jr9qnYBhv

3D Printing Fans @3DPrintingFans2:30 PM – 20 Oct 2015 via TweetDeck 9 4

Aleph Objects to Release Flexystruder Tool Heads for LulzBot 3D Printer Lineup @ #I3DPConf 3dprint.com/101457/flexyst… https://t.co/V4ubYDvvRK

Tyler Benster @tbenst10:04 PM – 20 Oct 2015 via iOS 9 6
Beautiful titanium print by @3dsystemscorp #I3DPConf https://t.co/Ie21Cv0tls

DesignBox3D @DesignBox3D4:16 AM – 20 Oct 2015 via Twitter for iPhone 8 9

We’re at #I3DPConf in #SantaClara this week with our friends @ZMorph3d 😎 Stop by and see us #3DPrinting #Fabrication https://t.co/E1hLKqvr6z

Most favorited tweets
Preet Jesrani @PreetJesrani8:55 PM – 21 Oct 2015 via Twitter for iPhone 5 12
Many thanks to @3dprintindustry @RealityInc for stopping by to visit @ZMorph3d @Inside3DPrint #I3DPConf #3DPrinting https://t.co/vb35MIRZ3W

3D Printing News @3DPrintGirl2:38 PM – 23 Oct 2015 via TweetDeck 10 12

Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! 3dprint.com/102064/startup… https://t.co/WPhiwzVZjN

The UPS Store @TheUPSStore5:02 PM – 21 Oct 2015 via Twitter Web Client 2 10
Tomorrow at #I3DPConf our expert panel is ready to answer your questions! Submit yours using #3DPWeek pic.twitter.com/PsWXws8x5Y

la moustache ringard @SJGrunewald5:42 PM – 21 Oct 2015 via Twitter for iPhone 10 9

@GoEngineer 3D printed #halo figure. One piece on #objetconnex @Stratasys multi color and materials. #I3DPConf https://t.co/7Jr9qnYBhv

DesignBox3D @DesignBox3D4:16 AM – 20 Oct 2015 via Twitter for iPhone 8 9

We’re at #I3DPConf in #SantaClara this week with our friends @ZMorph3d 😎 Stop by and see us #3DPrinting #Fabrication https://t.co/E1hLKqvr6z

Tuan TRANPHAM @ttranpham1:58 AM – 23 Oct 2015 via Twitter for iPhone 5 7

Thanks to @Inside3DPrint for hosting a great metal #3Dprinting panel today | tuan.tranpham@arcam.com | #I3DPConf | https://t.co/NXlT7VKOuf

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Tweets from the Inside 3D Printing Conference in California #I3DPConf

Posted in 3D Plotting Scaffolds, 3D Printing for Medical Application, Biopolymer Blend Open Porous, BioPrinting in Regenerative Medicine, Conference Coverage with Social Media, tagged , , on October 25, 2015| Leave a Comment »

Tweets from the Inside 3D Printing Conference in California #I3DPConf

Reporter: Stephen J. Williams, Ph.D.

 

i3dpconf

@Inside3DPrint

Covering #3DPrinting + @Mecklermedia‘s Inside 3D Printing Conference and Expo series + Strategies for the 3rd Industrial Revolution #I3DPConf

inside3dprinting.com

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Shell Verified account ‏@Shell Oct 21

Is personal car ownership a thing of the past? Find out on Nov. 4.

57 retweets 100 favorites

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Promoted

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3D Printing News ‏@3DPrintGirl Oct 23

Congratulations to #Metamason, winner of the #StartupCompetition at #I3DPConf! http://3dprint.com/102064/startup-winner-metamason/ …

10 retweets 12 favorites

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Day two at #I3DPConf in Santa Clara had even more to see! http://3dprint.com/102085/i3dpconf-santa-clara-day-two/ …

2 retweets 1 favorite

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3D Printing Fans and 4 others follow

Tuan TRANPHAM ‏@ttranpham Oct 22

Thanks to @Inside3DPrint for hosting a great metal #3Dprinting panel today | tuan.tranpham@arcam.com | #I3DPConf |

5 retweets 7 favorites

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Mike Grauer Jr and 4 others follow

Inside3DPrint ‏@Inside3DPrint Oct 22 Santa Clara, CA

Congratulations @Metamason3D for winning 1st place in the #startup competition! #I3DPConf

2 retweets 6 favorites

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‘3D Printing Meets Human Needs’: 3D Systems Keynote Explores “3D Printing-Plus” http://3dprint.com/102036/3ds-keynote-i3dpconf/ … #I3DPConf

View photo

15 retweets 7 favorites

Are We 3D Printing a Copyright Free World? Speakers at #I3DPConf explore the possibilities for IP in a 3D world http://3dprint.com/101891/3d-printing-copyright-free/ …

7 retweets 5 favorites

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3D Printing News ‏@3DPrintGirl Oct 21

The UPS Store: Personal Service for Small Businesses http://3dprint.com/101867/the-ups-store-at-i3dpconf/ … #I3DPConf

6 retweets 7 favorites

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3D Forged favorited

Reality™ Institute ‏@RealityInc Oct 21

That’s a lot of 3D printed car parts! #I3DPConf #3Dprinting

4 retweets 5 favorites

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Preet Jesrani ‏@PreetJesrani Oct 21

Many thanks to @3dprintindustry @RealityInc for stopping by to visit @ZMorph3d @Inside3DPrint #I3DPConf #3DPrinting

3D Printing Industry, Inside3DPrint, ZMorph 3D Printer and 2 others

5 retweets 12 favorites

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3D Printing News ‏@My3DPrinting Oct 21

Today at #I3DPConf: Made in Space Keynote Examines AM Among the Stars http://3dprint.com/101804/made-in-space-keynote/ … #3DPrinting

5 retweets 2 favorites

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3D Forged ‏@3DForged Oct 21

Print, scan, fax? The @AIORobotics Zeus does it all. They might be the coolest guys at the #I3DPConf too.

3 retweets 4 favorites

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3D Forged ‏@3DForged Oct 21

At the UPS Store Booth @ #I3DPConf. When UPS jumps into a new technology, you know it’s a big deal. #3dprinting

4 retweets 5 favorites

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3D Forged ‏@3DForged Oct 21

Printed on the @Zortrax_3D M200… #I3DPConf #3dprinting

2 retweets 1 favorite

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3D Forged ‏@3DForged Oct 21

Tons of cool filament from Polymaker… wood filament, flexi filament and a ton more. #I3DPConf #3dprinting

1 retweet 1 favorite

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3D Forged ‏@3DForged Oct 21

Octave booth #I3DPConf #3dprinting

1 retweet 1 favorite

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Day 2 of #I3DPConf Santa Clara! Interested in stereolithography #3dprinting technology? Stop by booth 502 to see the Form 2 and learn more

3 retweets 4 favorites

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3D Forged ‏@3DForged Oct 21

Finally here at the Inside 3D Printing Conference! #I3DPConf

1 retweet 1 favorite

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3D Printing Industry follows

The UPS Store Verified account ‏@TheUPSStore Oct 21

Tomorrow at #I3DPConf our expert panel is ready to answer your questions! Submit yours using #3DPWeek

Play

2 retweets 10 favorites

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Inside3DPrint ‏@Inside3DPrint Oct 20 Santa Clara, CA

Thanks @MakePrintable for revealing our fortune! #3dprinting #I3DPConf

4 retweets 5 favorites

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3D Printing News and 1 other follow

3DMFGNews ‏@3DMFGNews Oct 15

The Largest #3DPrinting Event Worldwide, @Inside3DPrint | http://bit.ly/1WXLqpf  | #I3DPConf#addmfg #mfg #3dp

3 retweets 1 favorite

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Inside3DPrint ‏@Inside3DPrint Oct 15

CONFIRMED: SLM Solutions is GOLD SPONSOR of #I3DPConf Shanghai & Sao Paulo, SILVER SPONSOR of Singapore http://bit.ly/1mFc8n1  #3dprinting

0 retweets 0 favorites

MecklerMedia ‏@MecklerMedia Oct 15

Inspiring & relevant 2-day #medical track 4 #I3DPConf Santa Clara http://bit.ly/1Ms8C7x  (via @3DPrint_com) #3dprinting #3dmedicine

1 retweet 0 favorites

.@garethekeane of @QualcommVenture to judge #Startup Competition at next week’s #I3DPConf Santa Clara! http://bit.ly/1K7gzxe  #3dprinting

0 retweets 0 favorites

Greenfish Labs ‏@GreenfishLabs Oct 15

Come see Jarratt Carson’s session at @Inside3DPrint in Santa Clara, October 20-22! http://mcklr.me/1We4grG  #3dprinting #I3DPConf

1 retweet 5 favorites

Come to the #I3DPConf and listen the keynote of our Global Sales Manager Kunal Varma http://www.efesto.us/news/inside-3d-printing-santa-clara.html … #3dprint @Inside3DPrint

1 retweet 3 favorites

Tim Caffrey joins @TerryWohlers @ #I3DPConf’s Wohlers Associates Consulting Dinner http://mcklr.me/1hCKqYA 

0 retweets 0 favorites

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3D Printing News ‏@3DPrintGirl Oct 15

The Medical Track offers incredible sessions #3Dprinting sessions at #I3DPConf next week! http://3dprint.com/100840/medical-track-i3dpconf/ …

5 retweets 4 favorites

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Inside3DPrint ‏@Inside3DPrint Oct 15

#I3DPConf, @autodesk, @sffashiontech, & @sculpteo will take on #3DPWeekCA http://bit.ly/1jzrKti  #3dprinting

2 retweets 2 favorites

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STARTS NOW: Glimpse into the future of #3dprinting at today’s last #I3DPConf Zhuhai session http://bit.ly/1LbfIgM  @Recycling_Times @tbenst

0 retweets 2 favorites

.@IDCAP Maggie Tan’s session ‘China #3dprinting Market’ starts NOW @ #I3DPConf Zhuhai! http://bit.ly/1VJbyBL  @Recycling_Times

0 retweets 1 favorite

Inside3DPrint ‏@Inside3DPrint Oct 14

iPrint 3D Expo pwrd by #I3DPConf is about to start! Expo hall opens at 9am http://bit.ly/1N3wL7s  @Recycling_Times #3dprinting

0 retweets 1 favorite

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Inside3DPrint ‏@Inside3DPrint Oct 14

#I3DPConf Mumbai Keynote #business track: @ImaginariumRao of @Imaginarium_Ind http://bit.ly/1VR2hYr  #3dprinting

1 retweet 1 favorite

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Industry experts to talk #3dprinting #startups & #IP at #I3DPConf Santa Clara in #business track http://bit.ly/1hgqFFd 

2 retweets 0 favorites

Get excited for #I3DPConf Mumbai’s #Design Challenge http://bit.ly/1Qt1JXc  (via @3DPrint_com) #3dprinting @divbyztech @inkjet_india

2 retweets 2 favorites

Mumbai: MecklerMedia’s Inside 3D Printing Design Challenge Kicks Off Excitement for Conference & Expo http://3dprint.com/100662/mumbai-design-challenge/ … #I3DPConf

2 retweets 0 favorites

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Fargo 3D Printing ‏@Fargo3DPrinting Oct 14

No show this week, but an update about what we’re up to. If you’re in Santa Clara for #I3DPConf, come say hi! http://youtu.be/NjKe1mxIMdo?a 

1 retweet 1 favorite

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COUNTDOWN: #I3DPConf Santa Clara is six days away! http://mcklr.me/1PhB5zL  #3Dprinting

0 retweets 0 favorites

Emily Gilbert ‏@EmilyGi52769653 Oct 14

Emily Gilbert Retweeted Inside3DPrint

RT http://twitter.com/Inside3DPrint/status/654325819825721344 … Learn about emerging #3dprinting tech in #prosthetics & #orthodontics w/ #I3DPConf San… http://bit.ly/1hgqFFd 

Emily Gilbert added,

Inside3DPrint @Inside3DPrint

Learn about emerging #3dprinting tech in #prosthetics & #orthodontics w/ #I3DPConf Santa Clara #medical track pass http://bit.ly/1hgqFFd

0 retweets 1 favorite

#I3DPConf Santa Clara’s reach is global: 32 US States, 23 Countries to be represented http://bit.ly/1Lo8yLD  #3dprinting

0 retweets 0 favorites

Start 3D Printing ‏@3Dprintbeginner Oct 14

Attend the largest #3dprinting event worldwide, @Inside3DPrint ‘s #I3DPConf in Mumbai http://mcklr.me/1JqxAFD 

2 retweets 1 favorite

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Brian Federal ‏@brianfederal Oct 14

Check out #HP Sprout at Inside 3D Printing Santa Clara Oct 20th New Education Computing https://www.youtube.com/watch?v=s6lUJKGAWko … #3dprinting #I3DPConf

1 retweet 1 favorite

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Join us in Santa Clara October 20th-11:15 for 3D Printing in Education: Emerging Curriculum and Delivery Models #3dprinting #I3DPConf

1 retweet 1 favorite

MecklerMedia ‏@MecklerMedia Oct 14

LAST CHANCE for #I3DPConf Mumbai early bird pricing. Ends midnight IST http://bit.ly/1QXsy5Z  #3dprinting

0 retweets 0 favorites

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Mike Grauer Jr and 4 others follow

Inside3DPrint ‏@Inside3DPrint Oct 13

FRIDAY, #I3DPConf Zhuhai: ‘Microeconomics of #3DPrinting in Asia: How it Actually Happens’ http://bit.ly/1LsLObk 

0 retweets 0 favorites

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Show #I3DPConf Santa Clara your #creativity w/ #CreativeForce #Design Competition http://bit.ly/1Oag9xS  #3dprinting #gartel3d @GARTEL

6 retweets 5 favorites

Meet #3dprinting industry experts & key exhibitors @ #I3DPConf Santa Clara’s Pre-Conference #Networking Dinner http://mcklr.me/1hGzrNX 

0 retweets 0 favorites

GoEngineer ‏@GoEngineer Oct 13

Be sure to visit GoEngineer Booth 315. #I3DPConf http://fb.me/6MkqhBLPa 

1 retweet 1 favorite

Yash Rane ‏@raneyash02 Oct 13

Yash Rane Retweeted Inside3DPrint

Trending up for upcoming #i3dpconf at Mumbai. Here me speaking 3d for #Chizelprint

Yash Rane added,

Inside3DPrint @Inside3DPrint

.@ChizelPrints Director & CEO @raneyash02 to speak at #I3DPConf Mumbai http://mcklr.me/1HS9sYr  #3dprinting

0 retweets 1 favorite

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WYNIT 3D ‏@WYNIT3D Oct 13

Our pumpkin army continues to grow. Pick one up at @Inside3DPrint Santa Clara. #3dprint #I3DPConf #Wynit3d

1 retweet 2 favorites

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LAST CHANCE: #I3DPConf Santa Clara price break ends tonight, 11:59PM PST! http://bit.ly/1Lo8yLD  #3dprinting

1 retweet 0 favorites

#I3DPConf Santa Clara to hold sessions on #filaments w/ the #manufacturing track pass http://bit.ly/1hgqFFd  #3dprinting

0 retweets 0 favorites

.@Inside3DPrint Slated for Australia in May 2016, at @NMWAustralia (via @3DPrint_com) http://mcklr.me/1K0g1sS  #3dprinting #I3DPConf

0 retweets 0 favorites

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3DMFGNews ‏@3DMFGNews Oct 12

The Largest #3DPrinting Event Worldwide, @Inside3DPrint | http://bit.ly/1WXLqpf  | #I3DPConf#addmfg #mfg #3dp

2 retweets 2 favorites

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Discuss the latest in #3dfashion and #wearables at #I3DPConf Santa Clara’s #art track http://bit.ly/1hgqFFd  #3dprinting

0 retweets 0 favorites

Inside3DPrint ‏@Inside3DPrint Oct 12

New to #I3DPConf Santa Clara: #CreativeForce #Design Competition w/ @GARTEL http://mcklr.me/1LSiw27  #3dprinting #gartel3d

1 retweet 2 favorites

Start 3D Printing ‏@3Dprintbeginner Oct 12

Meet other #3dprinting professionals at @Inside3DPrint Santa Clara Networking Dinner http://mcklr.me/1EphoWp  #I3DPConf

2 retweets 2 favorites

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Inside3DPrint ‏@Inside3DPrint Oct 11

Competitors & Judges Announced for #I3DPConf #3dprinting #Startup Competition http://bit.ly/1PkTez5 

0 retweets 0 favorites

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#3dprinted #art, #architecture, & #fashion come together w/ #I3DPConf Santa Clara’s #art track pass http://bit.ly/1hgqFFd  #3dprinting

0 retweets 0 favorites

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3D Printing News ‏@3DPrintGirl Oct 10

MecklerMedia’s #I3DPConf Slated for Australia in May 2016, at National Manufacturing Week http://3dprint.com/99553/inside-3d-printing-aus-2016/ …

5 retweets 2 favorites

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#I3DPConf Santa Clara Pre-Conference #Networking Dinner? $49. Harvesting lifelong industry connections? Priceless http://mcklr.me/1hGzrNX 

1 retweet 1 favorite

#I3DPConf Santa Clara attendees get discount on @LevisStadium tour http://mcklr.me/1LfaiEV  @SuperBowl @49ers #3dprinting

0 retweets 0 favorites

HOURS LEFT to save on #I3DPConf Zhuhai registration. Ends 11:59pm CST #3dprinting http://mcklr.me/1ED4ZbV 

0 retweets 0 favorites

Going to #I3DPConf Mumbai this December? Register before October 15 to save http://bit.ly/1QXsy5Z  #3dprinting

0 retweets 0 favorites

3D Print Week CA ‏@3dprintweekca Oct 9

.@3DPrinterOS, @AstroPrint3D, & @Metamason3D on long list of #I3DPConf Santa Clara #Startup Competition participants http://bit.ly/1VtmZTj 

0 retweets 1 favorite

MecklerMedia ‏@MecklerMedia Oct 9

Register for @Inside3DPrint Santa Clara by Oct 14 for early bird pricing http://bit.ly/1Lo8yLD  #3dprinting #I3DPConf

0 retweets 0 favorites

Inside3DPrint ‏@Inside3DPrint Oct 9

.@49ers are giving #I3DPConf CA attendees a behind-the-scenes look @ #SuperBowl50‘s @LevisStadium! http://mcklr.me/1LfaiEV@SuperBowl … #3dprinting

0 retweets 0 favorites

Competing Startups & Judges Announced for #I3DPConf #Startup Competition http://bit.ly/1MgEN9X  (via @3DPrint_com) #3dprinting

0 retweets 0 favorites

Imaginarium India ‏@Imaginarium_Ind Oct 8

Imaginarium India Retweeted Inside3DPrint

Inside 3D printing Design Challenge- think, learn, make, and win! http://bit.ly/1VDQiT8  #I3DPCONF #3dprinting

Imaginarium India added,

Inside3DPrint @Inside3DPrint

Deadline 4 #I3DPConf Mumbai Design Challenge: 10/29 http://bit.ly/1VDQiT8  #3dprinting @Imaginarium_Ind @divbyztech

1 retweet 3 favorites

So excited about upcoming #I3DPCONF in Zhuhai #innovation #tech #3dprinting

0 retweets 13 favorites

Mafe ‏@Mafefly Oct 8

Inside #3DPrinting Conference & Expo. Santa Clara October 20-22, 2015 #I3DPConf http://inside3dprinting.com/santa-clara/2015 …

0 retweets 1 favorite

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Inside3DPrint ‏@Inside3DPrint Oct 8

LAST 2 DAYS to save on #I3DPConf Shanghai passes http://mcklr.me/1TWiAC8  #3dprinting

0 retweets 0 favorites

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Inside3DPrint ‏@Inside3DPrint Oct 8

#I3DPConf & @ReedExhibitions partner for @NMWAustralia in Sydney, May 2016 http://bit.ly/1L1El1z  #3dprinting

0 retweets 0 favorites

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3D mapping of genome in combine FISH and RNAi

Posted in 3D Plotting Scaffolds, Academic Publishing, Advanced Drug Manufacturing Technology, tagged , , , on October 1, 2015| Leave a Comment »

3D mapping of genome in combine FISH and RNAi

Larry H. Bernstein, MD, FCAP, Curator

LPBI

Cellular factors that shape the 3D landscape of the genome identified

http://www.nih.gov/news/health/aug2015/nci-13.htm

Researchers, using novel large-scale imaging technology, have mapped the spatial location of individual genes in the nucleus of human cells and identified 50 cellular factors required for the proper three-dimensional (3D) positioning of genes. These spatial locations play important roles in gene expression, DNA repair, genome stability, and other cellular activities. The study, by scientists at the National Cancer Institute (NCI), part of the National Institutes of Health, appeared August 13, 2015, in Cell.

One of the fundamental properties of the genomes of higher organisms is the non-random arrangement of DNA in the cell nucleus. Researchers have long known that most genes occupy preferred 3D positions in the nucleus and that the location of genes matters for their function, but it has been difficult to determine the molecular players and mechanisms that determine the positions. Although genes can be visualized routinely and their positions determined using fluorescence in situ hybridization, or FISH, this mapping method has traditionally been limited to the analysis of a few samples at a time and cannot be used for large-scale genome mapping.

NCI researchers, in close collaboration with NCI’s High-Throughput Imaging Facility, which was established earlier this decade, have developed a method called HIPMap (High-throughput Imaging Position Mapping) that makes the large-scale determination of 3D gene positions possible. This method uses an optimized FISH detection protocol, fully automated microscopy, and combines it with sophisticated computational image analysis that delivers high-precision gene mapping information for thousands of samples in a single experiment.

In the study, NCI researchers, led by Tom Misteli, Ph.D., associate director, NCI Center for Cancer Research, used HIPMap and a method known as RNA interference (RNAi) knockdown to screen nearly 700 proteins in the nucleus to identify those that are involved in the 3D positioning of several human genes. RNAi knockdown uses RNA molecules to block the production of specific proteins in cells.

By collecting data continuously from automated microscopes for 27 days and then analyzing more than three million data points, the scientists were able to identify 50 cellular factors that determine the location of genes in the cell nucleus. This list provides the basis for further investigation of the molecular mechanisms of genome organization.

“The importance of HIPMap is that it is a starting point for numerous applications, including cancer biology,” said Misteli. “In addition to addressing basic questions about the mechanisms of how genomes are organized in intact cells, the ability to map gene positions in a large number of samples and cells has already been used to detect very rare chromosome translocation events in cancer and to ask what cellular factors determine where chromosomes break.”  During translocations, chromosomes break and reattach, which can cause the fusion of otherwise unconnected genes, resulting in hybrid genes whose protein products may contribute to the development of cancer.

As an example of the implications of HIPMap, Misteli pointed to a study from his lab published last month (Burman et al., Genes and Development. July 1, 2015).  In that study researchers used a method derived from HIPMap to probe mechanisms that contribute to the susceptibility of chromosomes to break and form a cancer-causing translocation between the NPM1 gene and the ALK gene in a cancer known as anaplastic large cell lymphoma. Another possible application of HIPMap is in cancer diagnostics. The researchers have previously shown that some genes assume distinct positions in cancer. As a result, the 3D positions of genes could be used as diagnostic markers in diseases such as breast cancer and prostate cancer.

“HIPMap will be a powerful tool in many ongoing efforts to map the genome in 3D space and to translate the findings from these studies to cancer biology,” Misteli concluded.

About the National Institutes of Health (NIH): NIH, the nation’s medical research agency, includes 27 Institutes and Centers and is a component of the U.S. Department of Health and Human Services. NIH is the primary federal agency conducting and supporting basic, clinical, and translational medical research, and is investigating the causes, treatments, and cures for both common and rare diseases. For more information about NIH and its programs, visit www.nih.gov.

NIH…Turning Discovery Into Health®

Reference

Shachar S, Voss TC, Pegoraro G, Sciascia N, Misteli T. Identification of Gene Positioning Factors Using High-throughput Imaging Mapping. Cell. August 13, 2015. DOI: 10.1016/j.cell.2015.07.035.

Stephen J. Williams, PhD

It is interesting they were able to complete this work and develop this technology which I see has other applications than which they suggested. It has been shown how certain factors (HIRAII) accumulate in certain areas of the chromatin during earliest stages of transformation and facilitate massive chromatin remodeling. That genetic information is spatially regulated, particular specific genes, and their technology can map it in a way I feel which is more accurate than probe methodologies or common sequencing methodologies is exciting news. however it may be difficult to use this as an early detection platform, unless it is done post-biopsy. A proceedure, possibly sensor based would need to be developed as well an an invitral imaging methodology.
The particular interesting application I would find would be the detection of insertion sites of genetic therapy. Currently it requires a long procedure involving knowledge of flanking sequences but this mapping procedure would help greatly, especially as I see it in determining insertion sites in development of personalized CART therapy. I would be interested if this group have been able to transfect in a gene and use their HIMAP to determine the spatial insertion site. Will be nice to see this work evolve..

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