Feeds:
Posts
Comments

Posts Tagged ‘Public Library of Science’


Reporter:  Aviva Lev-Ari, PhD, RN

Call for Open-Access Publishing in Genomics

January 14, 2013

SAN DIEGO (GenomeWeb News) – Open-access datasets, software, and bioinformatics strategies have become more or less de rigueur in genomics research.

But the field may also be poised to change the way other sorts of information from scientific studies is conveyed to other researchers and to the broader public, according to open-access proponent Michael Eisen, a computational and evolutionary biology researcher at the University of California, Berkeley.

Eisen, a Public Library of Science co-founder, spoke during the morning plenary session here at the International Plant and Animal Genome Conference.

In his presentation, he argued that the inability to freely and unreservedly access the full text of all genome studies performed to date may have led to missed opportunities for the field.

Using the bacteriophage phiX174 genome sequence as an example, he proposed that the general thinking in the genomics field has developed in ways that promote open-access to sequence data and related software. But, he said, the same type of access is not necessarily available for those interested in delving into the details and rationale behind genomics studies, since the corresponding papers may not be accessible in an open-access format.

The UK Medical Research Council Laboratory of Molecular Biology‘s Frederick Sanger and colleagues described the phiX174 sequence in 1977, in a publication that’s generally considered to be the first genome paper. The sequence data presented in that study is now freely available, Eisen explained, in part owing to the advent of sequence databases such as the European Molecular Biology Laboratory Nucleotide Sequence database or the National Center for Biotechnology Information’s sequence database, GenBank.

During the past decade or more, funding agency requirements and pressure from within the genomics community have contributed to the widespread adoption of these and other public genomics resources and repositories.

As these databases have grown and become accepted within the genomics community, Eisen argued that they have spurred the development of computational methods for analyzing genome sequences and datasets that may not have existed otherwise. “Imagine where we would be had we not made the fortunate decision to liberate genome sequences,” he said.

But analogous strategies for combing through text from genomics studies in their entirety have not developed in the same manner, according to Eisen, who noted that the text of the phiX174 genome paper remains behind a pay wall.

“We’ve allowed [journal access] and [data access] to follow very different fates,” said Eisen, who says there are ways to use the information housed within the scientific literature more easily and productively.

He urged attendees to consider publishing their own work in open-access publications. Beyond that, though, Eisen also noted that the community is well positioned to influence the ways in which research information is disseminated, since genomics data increasingly serves as a resource for other spheres of research.

 

 

Read Full Post »


Reporter: Aviva Lev-Ari, PhD, RN

 

Synthetic Biology

This collection aims to highlight PLOS ONE‘s role in the emerging interdisciplinary field of synthetic biology. The collection has its roots in PLOS ONE‘s very first issue, which included two publications from the field and since then, the number of synthetic biology articles published by the journal has grown steadily. As the field continues to develop, this collection will be updated to include new publications, thereby tracking the evolution of this dynamic research area.

Synthetic biology occurs at the intersection of a number of traditional disciplines, including biology, chemistry, and engineering. It aims to create biological systems that can be programmed to do useful things such as producing drugs and biofuel. The interdisciplinary nature of synthetic biology can make it difficult to publish in traditional journals. PLOS ONE‘s broad scope, however, allows for the publication of work crossing many traditional research boundaries, making it an ideal venue for many different types of synthetic biology publications. In addition, the journal’s focus on rigorous peer review without considering impact has made it possible to publish a body of articles that truly reflects the multifaceted nature of this research area.

One overarching theme of synthetic biology is standardization, which can only be achieved through concerted community effort. To this end, each article published in PLOS ONE can be the start of a lively conversation. The ability to comment on articles provides the community with a means to engage in a dialogue focused on specific articles, and the “Share this Article” feature allows readers to quickly send an article they find interesting to their entire networks, because all the content is openly accessible.

Articles in the Synthetic Biology Collection are presented in order of publication date and new articles will be added as they are published. PLOS ONE welcomes submissions in this field.

Collection Citation: Synthetic Biology (2012) PLOS Collections:http://www.ploscollections.org/syntheticbiology

Image Credit: Ivan Morozov (Virginia Bioinformatics Institute)

SOURCE

http://www.ploscollections.org/article/browseIssue.action?issue=info:doi/10.1371/issue.pcol.v02.i18

PLOS ONE Launches Synthetic Biology Collection

By Rachel Bernstein
Posted: August 15, 2012

Today PLOS ONE is happy to announce the launch of the Synthetic Biology Collection, including over 50 papers published in the last six years that illustrate the many facets of this dynamically evolving research area.

Synthetic biology is an innovative emerging field that exists at the intersection of many traditional disciplines, including biology, chemistry, and engineering, with aims to create biological systems that can be programmed to do useful things like produce drugs or biofuels, among other applications. Despite its potential, the heavily interdisciplinary nature of the research can make it difficult to publish in traditional discipline-specific journals.

However, PLOS ONE’s broad scope allows for the publication of work crossing many traditional research boundaries, making it an ideal venue for many different types of synthetic biology research. For example, the papers in the collection cover topics including DNA synthesis and assembly, standardized biological “parts” akin to interchangeable mechanical parts, protein engineering, and complex network and pathway analysis and modeling, as described in theCollection Overview written by collection editors Jean Peccoud of Virginia Tech and Mark Isalan of the Centre for Genomic Regulation.

The Collection has roots in PLOS ONE’s very first issue, which included two publications from the field. Since then, the number of synthetic biology articles published in the journal has grown steadily. The collection launched today highlights selected synthetic biology articles published in PLOS ONE since 2006, and it is intended to be a growing resource that will be updated regularly with new papers as the field continues to grow and develop.

Collection Citation: Synthetic Biology (2012) PLOS Collections:http://www.ploscollections.org/syntheticbiology

Image Credit: Ivan Morozov (Virginia Bioinformatics Institute)

SOURCE

http://blogs.plos.org/everyone/2012/08/15/plos-one-launches-synthetic-biology-collection/

The PLOS ONE Synthetic Biology Collection: Six Years and Counting

Jean Peccoud, Mark Isalan

PLoS ONE:
Published 15 Aug 2012 | info:doi/10.1371/journal.pone.0043231

The PLOS ONE Synthetic Biology Collection: Six Years and Counting 

Jean Peccoud1,2*, Mark Isalan3

1 Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, Virginia, United States of America, 2 Center for Systems Biology of Engineered Tissues, Institute for Critical Technologies and Applied Science, Virginia Tech, Blacksburg, Virginia, United States of America, 3 EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG) and UPF, Barcelona, Spain

Abstract 

Since it was launched in 2006, PLOS ONE has published over fifty articles illustrating the many facets of the emerging field of synthetic biology. This article reviews these publications by organizing them into broad categories focused on DNA synthesis and assembly techniques, the development of libraries of biological parts, the use of synthetic biology in protein engineering applications, and the engineering of gene regulatory networks and metabolic pathways. Finally, we review articles that describe enabling technologies such as software and modeling, along with new instrumentation. In order to increase the visibility of this body of work, the papers have been assembled into the PLOS ONE Synthetic Biology Collection (www.ploscollections.org/synbio). Many of the innovative features of the PLOS ONE web site will help make this collection a resource that will support a lively dialogue between readers and authors of PLOS ONE synthetic biology papers. The content of the collection will be updated periodically by including relevant articles as they are published by the journal. Thus, we hope that this collection will continue to meet the publishing needs of the synthetic biology community.

Citation: Peccoud J, Isalan M (2012) The PLOS ONE Synthetic Biology Collection: Six Years and Counting. PLoS ONE 7(8): e43231. doi:10.1371/journal.pone.0043231

Editor: Wei Ning Chen, Nanyang Technological University, Singapore

 

Received: May 23, 2012; Accepted: July 16, 2012; Published: August 15, 2012

Copyright: © 2012 Peccoud, Isalan. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funding: JP is supported by National Science Foundation Awards 0850100 and 0963988 and by grants R01-GM078989 and R01-GM095955 from the National Institutes of Health. MI is funded by FP7 ERC 201249 ZINC-HUBS, Ministerio de Ciencia e Innovacion grant MICINN BFU2010-17953 and the MEC-EMBL agreement. The funders had no role in the preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

* E-mail: peccoud@vt.edu

Introduction

Synthetic biology is an emerging transdisciplinary field at the intersection between many engineering and scientific disciplines such as biology, chemical engineering, chemistry, electrical engineering, or computer science. The scientific milestone that inspired the development of synthetic biology is often regarded as the description of two artificial gene networks in the same issue of Nature in 2000 [1][2]. However, the year 2004 marks the emergence of synthetic biology as a scientific community. This is the year of the first synthetic biology conference, the first iGEM competition –where students compete to build biological systems (http://igem.org/) ― and the creation of the synthetic biology page on Wikipedia. Two years later, the first issue of PLOS ONE included two synthetic biology articles [3][4], marking the beginning of a trend. Since then, PLOS ONE has published a large number of articles covering all aspects of the field. Synthetic biologists resolutely push the limits of their specialties in ways that few established journals have been able to appreciate. Since the result is often more “how to build something that works” rather than primary biological insight, the papers can be hard to place in classical journals. Many synthetic biology authors have benefited from the innovative PLOS ONE editorial policy to publish scientifically sound research, irrespective of its anticipated significance.

The purpose of this article is to introduce the PLOS ONE Synthetic Biology Collection (www.ploscollections.org/synbio/). The collection highlights selected synthetic biology articles published in PLOS ONE since 2006, putting them together in one place for easy perusal. The website is intended to be a growing resource that will be updated regularly.

We review the collection here by organizing it into some broad categories: DNA synthesis and assembly, Biological parts, Protein engineering, Networks and pathways, Synthetic life, Software and modeling, and Instruments. The classification is our own; since many synthetic biology papers cited in this review span more than one category, it was sometimes difficult to assign them to one category rather than another. Nonetheless, this structure should aid in navigating the 50+ papers currently included in the collection.

Summary of Papers Included in the Collection 

DNA Synthesis and Assembly

Synthetic biology projects often begin with the assembly of complicated, multi-component gene constructs. Therefore, both DNA assembly and cloning technologies are critical enablers of synthetic biology. Not surprisingly, many recent PLOS ONE papers propose methods to improve the efficiency of the fabrication step of synthetic biology projects. For example, Golden Gate Cloning [5] is a one-step DNA assembly protocol that can join at least nine distinct DNA fragments into one plasmid vector. The technique employs type IIs restriction enzymes that cut DNA at some distance from their cognate DNA-binding site, thus allowing flexibility and uniqueness in the compatible sticky ends that are generated. A related technique is GoldenBraid Assembly [6], that also uses type IIs restriction enzymes, but applies them iteratively to standardized DNA parts (see the ‘Biological parts’ section below). This allows the indefinite growth of reusable gene modules. Similarly, type IIs restriction enzymes have been used to make a hierarchical modular cloning system aimed at making eukaryotic multigene constructs [7].

‘One-pot’ assembly and cloning systems are being developed by many groups, and the ideal systems use as few standardized components as possible. Circular polymerase extension cloning (CPEC) fits into this category, using a single polymerase to assemble and clone multiple inserts with any vector, in a one-step in vitro reaction [8]. Alternatively, successive hybridization assembling (SHA) also employs a single reaction in vitro [9].

As well as cloning one desired multi-component construct, many projects require degenerate cloning or mutagenesis to make combinatorial libraries of gene variants. The OmniChange technique, which simultaneously saturates five independent codons, has therefore been developed to generate full-length gene libraries with 5 degenerate NNK-codons while avoiding PCR-amplification [10]. Large libraries of genetic sequences can be derived from oligonucleotides synthetized in a microarray, and later pooled in libraries from which more complex sequences can be derived [11]. By combining linear DNA amplification and PCR, DNA libraries with hundreds to thousands of members can be synthesized.

PCR methods themselves can have certain limitations, such as difficulties in amplifying GC-rich DNA targets. One study optimized polymerase chain assembly (PCA) and ligase chain reaction (LCR) methods for the construction of two GC-rich gene fragments implicated in tumorigenesis, IGF2R and BRAF [12]. They found that LCR was superior and benefited from the addition of DMSO and betaine.

The many synthesis and assembly methods presented in the collection can be combined to streamline the fabrication steps of synthetic biology projects, by producing collections of standardized biological parts. Standard parts are themselves a distinctive feature of synthetic biology, as reviewed below.

Biological Parts

The Registry of Standard Biological Parts (www.partsregistry.org), based on the original vision of Tom Knight, is providing a rich collection of components for synthetic biology projects. Several articles in the PLOS ONE collection reflect the importance of this resource. For example, a global analysis of the Registry clone collection [13] helped identify certain discrepancies between the sequences recorded in the database and the physical sequences of some clones in the collection. These results prompted a change in the quality control of the submissions to the Registry that has greatly improved the overall quality of the collection. Moreover, the analysis of parts usage patterns led to organizational guidelines that may help design and manage these new types of scientific resources. As most parts in the registry are for prokaryotes, a eukaryotic collection of 52 parts was developed and is available for distribution[14]. This includes multiple cloning sites (MCS), common protein tags, protein reporters and selection markers, amongst others. Furthermore, most of the parts were designed in a format to allow fusions that maintain the reading frame.

As well as standardized coding regions, synthetic biology projects require well-characterized promoters to achieve desired expression strengths. In one study, a single yeast promoter was mutated to make a fine-graded output range promoter library [15]. Transcription Activator-Like Orthogonal Repressors were then developed synthetically to control expression of these promoters in an orthogonal manner. Such orthogonality or ‘non-cross-reactivity’ is necessary for engineering larger synthetic gene circuits that do not interfere with the physiology of the biological chassis in which they operate. Mammalian synthetic promoters have also been developed by analyzing motifs found in highly active human promoters. Thus, by modulating the amount of sequences rich in GC and CpGs, custom designed promoters were obtained [16].

Finally, entirely de novo parts that are found nowhere in nature have been engineered to slot into biological systems. Using E. coli lacking conditionally essential genes, entirely new functional proteins were obtained from scaffolds of randomized 4-helix bundles, rescuing stalled growth [17]. Similarly, a synthetic ATP-binding protein, evolved entirely from non-natural sequences, was expressed in E. coli, altering the levels of intracellular ATP [18]. Protein engineering approaches are thus a potential source of many new parts, as well as forming a branch of synthetic biology in their own right.

Protein Engineering

Protein engineering can take many forms, from directed evolution methods to protein design. The PLOS ONE Synthetic Biology Collection includes a wide range of studies in this broad field.

Phage display is one of the classic tools of protein engineering, allowing combinatorial libraries of randomized proteins to be selected from the surface of bacteriophages. Phage display was used to generate a new class of binding proteins targeted to the pointed-end of actin [19]. These proteins, called synthetic antigen binders (sABs), were based on an antibody-like scaffold where sequence diversity is introduced into the binding loops using a new “reduced genetic code” phage display library.

An example of targeted protein design was the design of a dual reporter, Gemini [20]. Here, β-galactosidase (β-gal) α-fragment was fused to GFP, resulting in increased β-gal activity and some decrease in GFP sensitivity. GFP was also modified in a study where the ten proline residues of enhanced green fluorescent protein (EGFP) were replaced by (4R)- and (4S)-fluoroprolines (FPro) [21]. In this way, protein folding and stability could be tuned.

A promising advance in the field of engineering custom sequence-specific DNA-binding proteins is the use of Transcription Activator-Like (TAL) proteins. Modular TAL units specify A, C, G or T and can be concatenated to make long designer DNA-binding domains. Thus, Golden TAL Technology [22] has adapted Golden Gate Cloning [5] for engineering new TAL proteins. These were shown to function in human and plant cells and to target activation of both exogenous and endogenous genes, after fusion with a VP16 activation domain.

As well as single proteins, entire pathways can nowadays be engineered. Computational redesign was used to create new periplasmic binding proteins in plants, to act as biosensors in combination with a histidine kinase signaling cascade [23]. This resulted in transcription factor activation and ‘de-greening’ of plants in response to small-molecule stimuli. As can be seen from this example and the ones below, the move from single protein engineering to network engineering is one of the main driving forces in synthetic biology.

Networks and Pathways

One of the first, and now most-cited, synthetic biology papers in PLOS ONE was the study on fitness-induced attractor selection [3]. Here, a synthetic mutual inhibition gene network was built in E. coli, with two states, green (GFP) and red (RFP), that were mutually exclusive. By attaching a fitness pressure to one of the states (i.e. a gene required for growth in the absence of glutamine), the authors demonstrated that the cells switched stochastically into the fittest state, restoring growth. In other words, by changing to a glutamine-free medium, the red cells switched to green, even in the absence of formal signaling machinery. This work has important messages for potential new mechanisms in gene regulation, where underlying fitness pressures can ultimately determine how much a gene is expressed, simply according to need.

Other small bacterial networks have been built to include a heritable sequential memory switch, using the fim and hin inversion recombination systems [24], and an E. coli strain for use as a ‘chemical recording device’ [25]. In the latter, the authors created a synthetic chemically sensitive genetic toggle switch to activate appropriate fluorescent protein indicators (GFP, RFP) and along with a cell division inhibitor (minC). Moving to yeast, one example of network engineering was the reconstruction of a human p53-Mdm2 negative feedback module in S. cerevisiae [26]. In this example, many aspects of p53 regulation in mammals were maintained, such as Mdm2-dependent targeting of p53 for degradation, sumoylation at lysine 386 and further regulation of this process by p14ARF. In mammalian systems, a synthetic tetracycline regulator positive feedback loop was stably integrated and yielded a bimodal expression response because such cells can only be “OFF” or “ON” [27].

One unusual work in synthetic biology aimed to rewire and control cell shape in yeast, by changing the inputs into the α-factor pathway [28]. This pathway can give rise to multiple mating projections, upon prolonged activation. The authors tested genetic manipulations that ultimately gave rise to single or multiple projections, in the absence of the natural input, α-factor.

A group of papers in the collection explore ‘synthetic ecology’, where consortia of different cells interact to give patterns at a population level. For example, by engineering two strains of E. coli, one study was able to achieve synthetic biofilms with spatial self-organization [29]. The consortia achieved defined layered structures and had unexpected growth advantages. A second paper describes a systems composed of two quorum-sensing signal transduction circuits that allowed the authors to build a synthetic ecosystem where the population dynamics could be tuned by varying the environmental signals [30]. Third, quorum components were also used in a study which generated robust but unexpected oscillations in E. coli by building synthetic suicide circuits [31]. In fact, the quorum components proved to be unnecessary to achieve oscillations: there was a density-dependent plasmid amplification that gave rise to population-level negative feedback, ultimately resulting in the cycles. As in other areas of synthetic biology, the process of building systems often leads to surprises which can result in useful new engineering tools, or to a better understanding of the underlying biological processes [32].

Pathway engineering for the production of useful chemical or product synthesis is a major field within synthetic biology. For example, an engineered yeast that efficiently secretes penicillin was built by transplanting synthesis pathway components into a host that is more suited for pharmaceutical production [33]. Artemisinin derivatives are key components of malaria therapies and their synthesis is a high-profile goal of synthetic biology because extraction from slow-growing plants currently limits supply. Consequently, one study achieved high-level production of an artemisinin precursor in E. coli[34]. Another striking synthesis paper demonstrates a synthetic enzymatic pathway consisting of 13 enzymes for high-yield hydrogen production from starch and water [35]. Building such large systems is extremely challenging; as a result, these articles have received a lot of attention.

Synthetic Life

Synthetic life is among the most controversial of synthetic biology aims, and has received a lot of attention, even in the mainstream press. Public concerns of possible biological threats resulting from the misuse of these technologies prompted the development of new biosecurity policies [36].

One branch of this field is the de novo chemical synthesis and assembly of whole plasmids, viruses and genomes which are then transplanted into host cells. The pX1.0 plasmid is an example of a fully chemically-synthesized plasmid designed by calculating consensus sequences from 8 plasmids [37], while removing genes involved in antibiotic resistance and virulence. The plasmid not only replicated inE. coli, but could also self-transfer by conjugation into two other enterobacter species. A chemical synthesis approach was also used to construct whole genomes of bacteriophage G4 (around 10 kilobases in length), resulting in infectious viruses that could pass from one strain of E. coli to another[38].

One group has the ambitious long-term aim of building a synthetic chloroplast, and has begun by transplanting photosynthetic bacteria into eukaryotic cells to see whether they can achieve synthetic symbiosis [39]. Remarkably, the authors showed that some cyanobacteria were relatively harmless in zebrafish embryos, compared to E. coli. Furthermore, by engineering invasins into the cyanobacteria, they were able to invade and divide inside mammalian macrophages. Synthetic biology is only limited by our imagination, and one can speculate that entire free-living synthetic lifeforms could find their place in the collection in the not-too-distant future.

Software and Modeling

As the number of biological parts for synthetic biology increases, databases and design methods must evolve. For example, to help researchers search and retrieve biological parts, the Knowledgebase of Standard Biological Parts (SBPkb) is a Semantic Web resource for synthetic biology [40].

The collection also includes two articles presenting Computer Assisted Design software tools. Eugene is a human readable language to specify synthetic biological designs based on biological parts. It also provides a very expressive constraint system to drive the automatic creation of composite parts or devices from a collection of individual parts [41]. Alternatively, the Proto platform also provides a high-level biologically-oriented programming language [42]. Specifications are compiled from regulatory motifs, optimized, then converted into computational simulations for numerical verification.

Ultimately the design tools are only as good as the underlying mathematical models they rely on to make predictions of design behaviors. The collection includes a number of articles applying mathematical modeling approaches rooted in various engineering specialties to the design of synthetic genetic constructs.

Modeling gene networks is at the interface of systems and synthetic biology, and many PLOS ONE modeling papers aim to guide bioengineering projects. A recent example of adapting modeling for re-engineering properties into a system used a standardized synthetic yeast network from the In-vivo Reverse-engineering and Modeling Assessment (IRMA) [43]. Reverse engineering itself was used in a study which ultimately provided guidelines for chemotaxis pathway redesign [44]. Statecharts are used to describe dynamical systems, but have not been applied to gene networks. By doing so explicitly, one study was able to model network motifs and combine them in a complicated interlocked feed-forward loop network [45].

Two-component systems are common regulatory motifs in bacteria, and comprise a kinase that senses environmental signals together with a regulator that mediates the cell response. A recent study asked the question, “what happens if you add a third component that interacts with either of the other two?”[46]. Estimating the parameter space associated with a particular function is very valuable for guiding synthetic engineering approaches, as is determining whether a function is theoretically possible at all. For example, using a geometric argument, it was shown that, surprisingly, even monomer regulators can achieve bistability. This demonstrates the possibility of switch-like behavior in feedback autoloops without resorting to multimer regulators [47].

thumbnailFigure 1. Historical distribution of synthetic biology articles published by PLOS ONE.

This figure reports the number of articles in the collection published between 2006 and 2011. It shows a rapid growth of synthetic biology that reflects the growth of the journal and the increased familiarity of synthetic biologists with PLOS ONE.

doi:10.1371/journal.pone.0043231.g001

By combining experiments and computation, one study was able to derive design algorithms for altering synonymous codons in proteins, resulting in drastic expression differences of the same protein sequence[48]. For example, with DNA polymerase and single chain antibodies, expression could be predictably tuned to obtain concentrations ranging from undetectable to 30% of cellular protein. Importantly, using partial least squares regression, the authors noticed that favorable codons were predominantly those read by tRNAs that are most highly charged during amino acid starvation, not codons that are most abundant in highly expressed E. coli proteins. This is an important discovery for building genetic constructs that express appropriately inside the target cells.

Computation is a key function of biological networks and several studies in the collection present schemes to achieve this. The first is implemented at the level of chemical reactions and describes functions such as an inverter, an incrementer, a decrementer, a copier, a comparator, a multiplier, an exponentiator, a raise-to-a-power operation, and a logarithm in base two [49]. A key simplification is that the scheme uses only two reaction rates (“fast” and “slow”). A second study models a synthetic gene network to perform frequency multiplication [50]. Both of these studies assume deterministic relationships between input and outputs. Recently, the deterministic assumption has been challenged by experimental and theoretical works analyzing the importance of noise in the dynamics of gene networks [51]. This trend is illustrated in the collection by an article demonstrating that reliable timing of decision-making processes (choosing between multistable states) can be accomplished for large enough population sizes, as long as cells are globally coupled by chemical means [52]. Modeling can often reveal subtle non-intuitive designs, and, as a means of guiding synthetic biology, is likely to become an even larger field in the future.

thumbnailFigure 2. Relationships between article-level metrics.

For articles published between 2006 and 2009, there is a positive correlation between the number of times an article is cited in the scientific literature and the number of times it is viewed (A). For articles published between 2010 and 2012, there is a positive relationship between the number of views and the number of citations in the Mendeley social network (B). Metrics, such as number of views and citations in social media, give readers and authors an estimate of the scientific impact of individual articles well before they receive citations in scientific literature.

doi:10.1371/journal.pone.0043231.g002

Instruments

Nowadays, new technology and machinery is an important driving force for both primary biological discovery and for synthetic biology. A neat example is provided by the use of inkjet printer technology to provide low-cost high-resolution tools; a bacterial piezoelectric inkjet printer was designed to print out different strains of bacteria or chemicals in small droplets onto a flat surface at high resolution [53]. Another group used an inkjet for continuous dosing of diffusible regulators to a gel culture of E. coli, allowing 2D spatiotemporal regulation [54]. Precise spatiotemporal control of cells can also be achieved with microfluidics, and a recent report grew dividing yeast cells in a remarkable planar array [55]. Transient pulses of gene expression could be triggered by briefly inducing the GAL1 or MET3 promoters, resulting in coherent induction of cell division across the cell cluster. Other novel culture systems presented in the collection include the development of a 3-D cell culture system using a designer peptide nanofiber scaffold that self-assembled [4]. The peptide could be linked to functional motifs for cell adhesion, differentiation, and bone marrow homing for use with mouse adult neural stem cells.

The Synthetic Biology Collection: A Dynamic Community Resource Top

It is remarkable that the collection includes several articles originating from engineers and computer scientists who traditionally publish their work in conference proceedings rather than the journals available to life-scientists. PLOS ONE’s indifference to subject matter made it possible to publish an unprecedented body of articles that reflects the multi-faceted nature of synthetic biology. No less remarkable is the observation that PLOS ONE published several articles originating from iGEM projects[13][41][56].

Since 2006, the number of synthetic biology articles published by the journal has been growing steadily (Figure 1). This evolution is consistent with the social trends in synthetic biology that have been mapped in an interesting bibliometric analysis included in the collection [57]. This is an indication that the synthetic biology community is becoming more aware of the services provided by the journal. Looking forward, the collection will make it easier to identify synthetic biology articles among the quickly growing volume of articles published by the journal each day. The content of the collection will be updated periodically as new synthetic biology articles are published by the journal.

Although Journal Impact Factors are a widely-discredited form of evaluating the quality of individual papers, all too often they are still used. Thus, it is imperative to find a better alternative. One of the most exciting features of the PLOS ONE web site is the Metrics tab, displaying article-based metrics that can be used to assess the impact of individual articles. These metrics naturally include traditional indicators, such as the number of citations. The two articles of the collection published in 2006 have been cited 70 and 84 times so far. Almost all the articles published in 2007 and 2008 have received more than 10 citations. The lag between the publication of an article and its citation by others is well known. Fortunately, the Metrics tab also includes more innovative indicators that give the authors and readers alike a real-time estimate of the ‘impact’ of an article. The number of times an article is viewed is an important indicator. Since PLOS ONE is an online journal, all readers view articles online in one way or another. As a result, we hypothesized that the number of times an article was viewed should be a good predictor of the number of citations it will receive. Using data reported in Table S1, we analyzed the relationship between views and citation numbers for articles included in the collection that were published between 2006 and 2009. Figure 2 shows that there is a positive correlation between the two metrics. That relationship does not hold when including more recent articles because of a difference in timing between viewing and citing activities. Articles typically receive a substantial number of views in the first few months after publication, but it takes a few years before they are cited. The 20 articles of the collection published in 2011 have recorded a lot of views, but have not had the time to be cited in the literature yet.

A non-conventional form of citations displayed in the Metrics tab is the number of times an article is bookmarked in social media. We have reported the Mendeley (www.mendeley.com) data in Table S1.Figure 2 shows that there is a positive relationship between the number of views and the number of times articles are bookmarked in this network, at least for the most recent articles of the collection. Older articles are under-represented in Mendeley because this network was not available at the time these articles were published. It will be interesting to see if citations of the collection articles in social media will be a better predictor of citations in the scientific literature than the number of views.

One overarching theme of synthetic biology is standardization [58][59], which can only be achieved through concerted efforts by members of the community. The field has therefore been deeply influenced by the development of resources such as the Registry of Standard Biological Parts (www.partsregistry.org ). More recently, the development of SBOL, the Open Language for Synthetic Biology (www.sbolstandard.org) illustrates the need to agree on data formats suitable to the development of software tool chains necessary to support experimental efforts. Each article published in PLOS ONE can be the start of a lively conversation. The journal web site provides authors and readers alike with a detailed vision of community connections. The “Share this article” feature allows readers to quickly send an article they find interesting to their networks. The comments tab of the articles provides the community with means to engage in a dialogue focused on specific articles [5][35][48][55]. This feature can also be used by authors to provide updated information about the work presented in the article [13].

When working at its best, science should be an active conversation that keeps refining ideas. We believe that PLOS ONE provides the ideal venue to achieve this, and we hope that the collection will inspire further progress in synthetic biology. Ultimately, we hope that having a clear repository in PLOS ONE should further increase its attractiveness as a home for publishing synthetic biology.

Table S1.

Article-level statistics for the Synthetic Biology Collection.

(XLSX)

Author Contributions

Wrote the paper: JP MI.

References Top

  1. Elowitz MB, Leibler S (2000) A synthetic oscillatory network of transcriptional regulators. Nature 403: 335–338. FIND THIS ARTICLE ONLINE
  2. Gardner TS, Cantor CR, Collins JJ (2000) Construction of a genetic toggle switch in Escherichia coli. Nature 403: 339–342. FIND THIS ARTICLE ONLINE
  3. Kashiwagi A, Urabe I, Kaneko K, Yomo T (2006) Adaptive Response of a Gene Network to Environmental Changes by Fitness-Induced Attractor Selection. PLoS ONE 1: e49. FIND THIS ARTICLE ONLINE
  4. Gelain F, Bottai D, Vescovi A, Zhang S (2006) Designer Self-Assembling Peptide Nanofiber Scaffolds for Adult Mouse Neural Stem Cell 3-Dimensional Cultures. PLoS ONE 1: e119. FIND THIS ARTICLE ONLINE
  5. Engler C, Gruetzner R, Kandzia R, Marillonnet S (2009) Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes. PLoS One 4: e5553. FIND THIS ARTICLE ONLINE
  6. Sarrion-Perdigones A, Falconi E, Zandalinas S, Juárez P, Fernández-del-Carmen A, et al. (2011) GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules. PLoS ONE 6: e21622. FIND THIS ARTICLE ONLINE
  7. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S (2011) A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6: e16765. FIND THIS ARTICLE ONLINE
  8. Quan J, Tian J (2009) Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways. PLoS ONE 4: e6441. FIND THIS ARTICLE ONLINE
  9. Jiang X, Yang J, Zhang H, Zou H, Wang C, et al. (2012) In Vitro Assembly of Multiple DNA Fragments Using Successive Hybridization. PLoS ONE 7: e30267. FIND THIS ARTICLE ONLINE
  10. Dennig A, Shivange A, Marienhagen J, Schwaneberg U (2011) OmniChange: The Sequence Independent Method for Simultaneous Site-Saturation of Five Codons. PLoS ONE 6: e26222.FIND THIS ARTICLE ONLINE
  11. Svensen N, Díaz-Mochón JJ, Bradley M (2011) Microarray generation of thousand-member oligonucleotide libraries. PLoS ONE 6: e24906. FIND THIS ARTICLE ONLINE
  12. Jensen M, Fukushima M, Davis R (2010) DMSO and Betaine Greatly Improve Amplification of GC-Rich Constructs in De Novo Synthesis. PLoS ONE 5: e11024. FIND THIS ARTICLE ONLINE
  13. Peccoud J, Blauvelt MF, Cai Y, Cooper KL, Crasta O, et al. (2008) Targeted Development of Registries of Biological Parts. PLoS ONE 3: e2671. FIND THIS ARTICLE ONLINE
  14. Constante M, Grünberg R, Isalan M (2011) A Biobrick Library for Cloning Custom Eukaryotic Plasmids. PLoS ONE 6: e23685. FIND THIS ARTICLE ONLINE
  15. Blount BA, Weenink T, Vasylechko S, Ellis T (2012) Rational Diversification of a Promoter Providing Fine-Tuned Expression and Orthogonal Regulation for Synthetic Biology. PLoS ONE 7: e33279.FIND THIS ARTICLE ONLINE
  16. Grabherr M, Pontiller J, Mauceli E, Ernst W, Baumann M, et al. (2011) Exploiting Nucleotide Composition to Engineer Promoters. PLoS ONE 6: e20136. FIND THIS ARTICLE ONLINE
  17. Fisher M, McKinley K, Bradley L, Viola S, Hecht M (2011) De Novo Designed Proteins from a Library of Artificial Sequences Function in Escherichia Coli and Enable Cell Growth. PLoS ONE 6: e15364.FIND THIS ARTICLE ONLINE
  18. Stomel J, Wilson J, León M, Stafford P, Chaput J (2009) A Man-Made ATP-Binding Protein Evolved Independent of Nature Causes Abnormal Growth in Bacterial Cells. PLoS ONE 4: e7385. FIND THIS ARTICLE ONLINE
  19. Brawley C, Uysal S, Kossiakoff A, Rock R (2010) Characterization of Engineered Actin Binding Proteins That Control Filament Assembly and Structure. PLoS ONE 5: e13960. FIND THIS ARTICLE ONLINE
  20. Martin L, Che A, Endy D (2009) Gemini, a bifunctional enzymatic and fluorescent reporter of gene expression. PLoS One 4: e7569. FIND THIS ARTICLE ONLINE
  21. Steiner T, Hess P, Bae JH, Wiltschi B, Moroder L, et al. (2008) Synthetic Biology of Proteins: Tuning GFPs Folding and Stability with Fluoroproline. PLoS ONE 3: e1680. FIND THIS ARTICLE ONLINE
  22. Weber E, Gruetzner R, Werner S, Engler C, Marillonnet S (2011) Assembly of Designer TAL Effectors by Golden Gate Cloning. PLoS ONE 6: e19722. FIND THIS ARTICLE ONLINE
  23. Antunes M, Morey K, Smith J, Albrecht K, Bowen T, et al. (2011) Programmable Ligand Detection System in Plants through a Synthetic Signal Transduction Pathway. PLoS ONE 6: e16292. FIND THIS ARTICLE ONLINE
  24. Ham T, Lee S, Keasling J, Arkin A (2008) Design and Construction of a Double Inversion Recombination Switch for Heritable Sequential Genetic Memory. PLoS ONE 3: e2815. FIND THIS ARTICLE ONLINE
  25. Bhomkar P, Materi W, Wishart D (2011) The Bacterial Nanorecorder: Engineering E. coli to Function as a Chemical Recording Device. PLoS ONE 6: e27559. FIND THIS ARTICLE ONLINE
  26. Di Ventura B, Funaya C, Antony C, Knop M, Serrano L (2008) Reconstitution of Mdm2-Dependent Post-Translational Modifications of p53 in Yeast. PLoS ONE 3: e1507. FIND THIS ARTICLE ONLINE
  27. May T, Eccleston L, Herrmann S, Hauser H, Goncalves J, et al. (2008) Bimodal and Hysteretic Expression in Mammalian Cells from a Synthetic Gene Circuit. PLoS ONE 3: e2372. FIND THIS ARTICLE ONLINE
  28. Tanaka H, Yi T-M (2009) Synthetic Morphology Using Alternative Inputs. PLoS ONE 4: e6946.FIND THIS ARTICLE ONLINE
  29. Brenner K, Arnold F (2011) Self-Organization, Layered Structure, and Aggregation Enhance Persistence of a Synthetic Biofilm Consortium. PLoS ONE 6: e16791. FIND THIS ARTICLE ONLINE
  30. Hu B, Du J, Zou R-y, Yuan Y-j (2010) An Environment-Sensitive Synthetic Microbial Ecosystem. PLoS ONE 5: e10619. FIND THIS ARTICLE ONLINE
  31. Marguet P, Tanouchi Y, Spitz E, Smith C, You L (2010) Oscillations by Minimal Bacterial Suicide Circuits Reveal Hidden Facets of Host-Circuit Physiology. PLoS ONE 5: e11909. FIND THIS ARTICLE ONLINE
  32. Elowitz M, Lim WA (2010) Build life to understand it. Nature 468: 889–890. FIND THIS ARTICLE ONLINE
  33. Gidijala L, Kiel J, Douma R, Seifar R, van Gulik W, et al. (2009) An Engineered Yeast Efficiently Secreting Penicillin. PLoS ONE 4: e8317. FIND THIS ARTICLE ONLINE
  34. Tsuruta H, Paddon C, Eng D, Lenihan J, Horning T, et al. (2009) High-Level Production of Amorpha-4,11-Diene, a Precursor of the Antimalarial Agent Artemisinin, in Escherichia coli. PLoS ONE 4: e4489. FIND THIS ARTICLE ONLINE
  35. Zhang P, Evans B, Mielenz J, Hopkins R, Adams M (2007) High-Yield Hydrogen Production from Starch and Water by a Synthetic Enzymatic Pathway. PLoS ONE 2: e456. FIND THIS ARTICLE ONLINE
  36. Adam L, Kozar M, Letort G, Mirat O, Srivastava A, et al. (2011) Strengths and limitations of the federal guidance on synthetic DNA. Nat Biotechnol 29: 208–210. FIND THIS ARTICLE ONLINE
  37. Hansen L, Bentzon-Tilia M, Bentzon-Tilia S, Norman A, Rafty L, et al. (2011) Design and Synthesis of a Quintessential Self-Transmissible IncX1 Plasmid, pX1.0. PLoS ONE 6: e19912. FIND THIS ARTICLE ONLINE
  38. Yang R, Han Y, Ye Y, Liu Y, Jiang Z, et al. (2011) Chemical Synthesis of Bacteriophage G4. PLoS ONE 6: e27062. FIND THIS ARTICLE ONLINE
  39. Agapakis C, Niederholtmeyer H, Noche R, Lieberman T, Megason S, et al. (2011) Towards a Synthetic Chloroplast. PLoS ONE 6: e18877. FIND THIS ARTICLE ONLINE
  40. Galdzicki M, Rodriguez C, Chandran D, Sauro H, Gennari J (2011) Standard Biological Parts Knowledgebase. PLoS ONE 6: e17005. FIND THIS ARTICLE ONLINE
  41. Bilitchenko L, Liu A, Cheung S, Weeding E, Xia B, et al. (2011) Eugene – A Domain Specific Language for Specifying and Constraining Synthetic Biological Parts, Devices, and Systems. PLoS ONE 6: e18882. FIND THIS ARTICLE ONLINE
  42. Beal J, Lu T, Weiss R (2011) Automatic Compilation from High-Level Biologically-Oriented Programming Language to Genetic Regulatory Networks. PLoS ONE 6: e22490. FIND THIS ARTICLE ONLINE
  43. Marucci L, Barton D, Cantone I, Ricci M, Cosma M, et al. (2009) How to Turn a Genetic Circuit into a Synthetic Tunable Oscillator, or a Bistable Switch. PLoS ONE 4: e8083. FIND THIS ARTICLE ONLINE
  44. Luo J, Wang J, Ma T, Sun Z (2010) Reverse Engineering of Bacterial Chemotaxis Pathway via Frequency Domain Analysis. PLoS ONE 5: e9182. FIND THIS ARTICLE ONLINE
  45. Shin Y-J, Nourani M (2010) Statecharts for Gene Network Modeling. PLoS ONE 5: e9376. FIND THIS ARTICLE ONLINE
  46. Salvado B, Vilaprinyo E, Karathia H, Sorribas A, Alves R (2012) Two Component Systems: Physiological Effect of a Third Component. PLoS ONE 7: e31095. FIND THIS ARTICLE ONLINE
  47. Widder S, Macía J, Solé R (2009) Monomeric Bistability and the Role of Autoloops in Gene Regulation. PLoS ONE 4: e5399. FIND THIS ARTICLE ONLINE
  48. Welch M, Govindarajan S, Ness J, Villalobos A, Gurney A, et al. (2009) Design Parameters to Control Synthetic Gene Expression in Escherichia coli. PLoS ONE 4: e7002. FIND THIS ARTICLE ONLINE
  49. Senum P, Riedel M (2011) Rate-Independent Constructs for Chemical Computation. PLoS ONE 6: e21414. FIND THIS ARTICLE ONLINE
  50. Purcell O, di Bernardo M, Grierson C, Savery N (2011) A Multi-Functional Synthetic Gene Network: A Frequency Multiplier, Oscillator and Switch. PLoS ONE 6: e16140. FIND THIS ARTICLE ONLINE
  51. Balazsi G, van Oudenaarden A, Collins JJ (2011) Cellular decision making and biological noise: from microbes to mammals. Cell 144: 910–925. FIND THIS ARTICLE ONLINE
  52. Koseska A, Zaikin A, Kurths J, García-Ojalvo J (2009) Timing Cellular Decision Making Under Noise via Cell–Cell Communication. PLoS ONE 4: e4872. FIND THIS ARTICLE ONLINE
  53. Merrin J, Leibler S, Chuang JS (2007) Printing multistrain bacterial patterns with a piezoelectric inkjet printer. PLoS ONE 2: e663. FIND THIS ARTICLE ONLINE
  54. Cohen D, Morfino R, Maharbiz M (2009) A Modified Consumer Inkjet for Spatiotemporal Control of Gene Expression. PLoS ONE 4: e7086. FIND THIS ARTICLE ONLINE
  55. Charvin G, Cross FR, Siggia ED (2008) A microfluidic device for temporally controlled gene expression and long-term fluorescent imaging in unperturbed dividing yeast cells. PLoS One 3: e1468. FIND THIS ARTICLE ONLINE
  56. Hesselman MC, Odoni DI, Ryback BM, de Groot S, van Heck RG, et al. (2012) A multi-platform flow device for microbial (co-) cultivation and microscopic analysis. PLoS One 7: e36982. FIND THIS ARTICLE ONLINE
  57. Oldham P, Hall S, Burton G (2012) Synthetic biology: mapping the scientific landscape. PLoS One 7: e34368. FIND THIS ARTICLE ONLINE
  58. Endy D (2005) Foundations for engineering biology. Nature 438: 449–453. FIND THIS ARTICLE ONLINE
  59. Canton B, Labno A, Endy D (2008) Refinement and standardization of synthetic biological parts and devices. Nat Biotechnol 26: 787–793. FIND THIS ARTICLE ONLINE
SOURCE

Research Articles

A Multi-Platform Flow Device for Microbial (Co-) Cultivation and Microscopic Analysis

Matthijn C. Hesselman, Dorett I. Odoni, Brendan M. Ryback, Suzette de Groot, Ruben G. A. van Heck, Jaap Keijsers, Pim Kolkman, David Nieuwenhuijse, Youri M. van Nuland, Erik Sebus, Rob Spee, Hugo de Vries, Marten T. Wapenaar, Colin J. Ingham, Karin Schroën, Vítor A. P. Martins dos Santos, Sebastiaan K. Spaans, Floor Hugenholtz, Mark W. J. van Passel

PLoS ONE:
Published 14 May 2012 | info:doi/10.1371/journal.pone.0036982

Synthetic Biology: Mapping the Scientific Landscape

Paul Oldham, Stephen Hall, Geoff Burton

PLoS ONE:
Published 23 Apr 2012 | info:doi/10.1371/journal.pone.0034368

Rational Diversification of a Promoter Providing Fine-Tuned Expression and Orthogonal Regulation for Synthetic Biology

Benjamin A. Blount, Tim Weenink, Serge Vasylechko, Tom Ellis

PLoS ONE:
Published 19 Mar 2012 | info:doi/10.1371/journal.pone.0033279

Two Component Systems: Physiological Effect of a Third Component

Baldiri Salvado, Ester Vilaprinyo, Hiren Karathia, Albert Sorribas, Rui Alves

PLoS ONE:
Published 17 Feb 2012 | info:doi/10.1371/journal.pone.0031095

In Vitro Assembly of Multiple DNA Fragments Using Successive Hybridization

Xinglin Jiang, Jianming Yang, Haibo Zhang, Huibin Zou, Cong Wang, Mo Xian

PLoS ONE:
Published 26 Jan 2012 | info:doi/10.1371/journal.pone.0030267

The Bacterial Nanorecorder: Engineering E. coli to Function as a Chemical Recording Device

Prasanna Bhomkar, Wayne Materi, David S. Wishart

PLoS ONE:
Published 23 Nov 2011 | info:doi/10.1371/journal.pone.0027559

Chemical Synthesis of Bacteriophage G4

Ruilin Yang, Yonghua Han, Yiwang Ye, Yuchen Liu, Zhimao Jiang, Yaoting Gui, Zhiming Cai

PLoS ONE:
Published 16 Nov 2011 | info:doi/10.1371/journal.pone.0027062

OmniChange: The Sequence Independent Method for Simultaneous Site-Saturation of Five Codons

Alexander Dennig, Amol V. Shivange, Jan Marienhagen, Ulrich Schwaneberg

PLoS ONE:
Published 19 Oct 2011 | info:doi/10.1371/journal.pone.0026222

Microarray Generation of Thousand-Member Oligonucleotide Libraries

Nina Svensen, Juan José Díaz-Mochón, Mark Bradley

PLoS ONE:
Published 23 Sep 2011 | info:doi/10.1371/journal.pone.0024906

A Biobrick Library for Cloning Custom Eukaryotic Plasmids

Marco Constante, Raik Grünberg, Mark Isalan

PLoS ONE:
Published 25 Aug 2011 | info:doi/10.1371/journal.pone.0023685

Automatic Compilation from High-Level Biologically-Oriented Programming Language to Genetic Regulatory Networks

Jacob Beal, Ting Lu, Ron Weiss

PLoS ONE:
Published 05 Aug 2011 | info:doi/10.1371/journal.pone.0022490

GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules

Alejandro Sarrion-Perdigones, Erica Elvira Falconi, Sara I. Zandalinas, Paloma Juárez, Asun Fernández-del-Carmen, Antonio Granell, Diego Orzaez

PLoS ONE:
Published 07 Jul 2011 | info:doi/10.1371/journal.pone.0021622

Rate-Independent Constructs for Chemical Computation

Phillip Senum, Marc Riedel

PLoS ONE:
Published 30 Jun 2011 | info:doi/10.1371/journal.pone.0021414

Assembly of Designer TAL Effectors by Golden Gate Cloning

Ernst Weber, Ramona Gruetzner, Stefan Werner, Carola Engler, Sylvestre Marillonnet

PLoS ONE:
Published 19 May 2011 | info:doi/10.1371/journal.pone.0019722

Design and Synthesis of a Quintessential Self-Transmissible IncX1 Plasmid, pX1.0

Lars H. Hansen, Mikkel Bentzon-Tilia, Sara Bentzon-Tilia, Anders Norman, Louise Rafty, Søren J. Sørensen

PLoS ONE:
Published 18 May 2011 | info:doi/10.1371/journal.pone.0019912

Exploiting Nucleotide Composition to Engineer Promoters

Manfred G. Grabherr, Jens Pontiller, Evan Mauceli, Wolfgang Ernst, Martina Baumann, Tara Biagi, Ross Swofford, Pamela Russell, Michael C. Zody, Federica Di Palma, Kerstin Lindblad-Toh, Reingard M. Grabherr

PLoS ONE:
Published 18 May 2011 | info:doi/10.1371/journal.pone.0020136

Eugene – A Domain Specific Language for Specifying and Constraining Synthetic Biological Parts, Devices, and Systems

Lesia Bilitchenko, Adam Liu, Sherine Cheung, Emma Weeding, Bing Xia, Mariana Leguia, J. Christopher Anderson, Douglas Densmore

PLoS ONE:
Published 29 Apr 2011 | info:doi/10.1371/journal.pone.0018882

Towards a Synthetic Chloroplast

Christina M. Agapakis, Henrike Niederholtmeyer, Ramil R. Noche, Tami D. Lieberman, Sean G. Megason, Jeffrey C. Way, Pamela A. Silver

PLoS ONE:
Published 20 Apr 2011 | info:doi/10.1371/journal.pone.0018877

Standard Biological Parts Knowledgebase

Michal Galdzicki, Cesar Rodriguez, Deepak Chandran, Herbert M. Sauro, John H. Gennari

PLoS ONE:
Published 24 Feb 2011 | info:doi/10.1371/journal.pone.0017005

A Modular Cloning System for Standardized Assembly of Multigene Constructs

Ernst Weber, Carola Engler, Ramona Gruetzner, Stefan Werner, Sylvestre Marillonnet

PLoS ONE:
Published 18 Feb 2011 | info:doi/10.1371/journal.pone.0016765

A Multi-Functional Synthetic Gene Network: A Frequency Multiplier, Oscillator and Switch

Oliver Purcell, Mario di Bernardo, Claire S. Grierson, Nigel J. Savery

PLoS ONE:
Published 17 Feb 2011 | info:doi/10.1371/journal.pone.0016140

Self-Organization, Layered Structure, and Aggregation Enhance Persistence of a Synthetic Biofilm Consortium

Katie Brenner, Frances H. Arnold

PLoS ONE:
Published 09 Feb 2011 | info:doi/10.1371/journal.pone.0016791

Programmable Ligand Detection System in Plants through a Synthetic Signal Transduction Pathway

Mauricio S. Antunes, Kevin J. Morey, J. Jeff Smith, Kirk D. Albrecht, Tessa A. Bowen, Jeffrey K. Zdunek, Jared F. Troupe, Matthew J. Cuneo, Colleen T. Webb, Homme W. Hellinga, June I. Medford

PLoS ONE:
Published 25 Jan 2011 | info:doi/10.1371/journal.pone.0016292

De Novo Designed Proteins from a Library of Artificial Sequences Function inEscherichia Coli and Enable Cell Growth

Michael A. Fisher, Kara L. McKinley, Luke H. Bradley, Sara R. Viola, Michael H. Hecht

PLoS ONE:
Published 04 Jan 2011 | info:doi/10.1371/journal.pone.0015364

Characterization of Engineered Actin Binding Proteins That Control Filament Assembly and Structure

Crista M. Brawley, Serdar Uysal, Anthony A. Kossiakoff, Ronald S. Rock

PLoS ONE:
Published 12 Nov 2010 | info:doi/10.1371/journal.pone.0013960

Oscillations by Minimal Bacterial Suicide Circuits Reveal Hidden Facets of Host-Circuit Physiology

Philippe Marguet, Yu Tanouchi, Eric Spitz, Cameron Smith, Lingchong You

PLoS ONE:
Published 30 Jul 2010 | info:doi/10.1371/journal.pone.0011909

DMSO and Betaine Greatly Improve Amplification of GC-Rich Constructs in De Novo Synthesis

Michael A. Jensen, Marilyn Fukushima, Ronald W. Davis

PLoS ONE:
Published 11 Jun 2010 | info:doi/10.1371/journal.pone.0011024

An Environment-Sensitive Synthetic Microbial Ecosystem

Bo Hu, Jin Du, Rui-yang Zou, Ying-jin Yuan

PLoS ONE:
Published 12 May 2010 | info:doi/10.1371/journal.pone.0010619

Reverse Engineering of Bacterial Chemotaxis Pathway via Frequency Domain Analysis

Junjie Luo, Jun Wang, Ting Martin Ma, Zhirong Sun

PLoS ONE:
Published 09 Mar 2010 | info:doi/10.1371/journal.pone.0009182

Statecharts for Gene Network Modeling

Yong-Jun Shin, Mehrdad Nourani

PLoS ONE:
Published 23 Feb 2010 | info:doi/10.1371/journal.pone.0009376

An Engineered Yeast Efficiently Secreting Penicillin

Loknath Gidijala, Jan A. K. W. Kiel, Rutger D. Douma, Reza M. Seifar, Walter M. van Gulik, Roel A. L. Bovenberg, Marten Veenhuis, Ida J. van der Klei

PLoS ONE:
Published 15 Dec 2009 | info:doi/10.1371/journal.pone.0008317

How to Turn a Genetic Circuit into a Synthetic Tunable Oscillator, or a Bistable Switch

Lucia Marucci, David A. W. Barton, Irene Cantone, Maria Aurelia Ricci, Maria Pia Cosma, Stefania Santini, Diego di Bernardo, Mario di Bernardo

PLoS ONE:
Published 07 Dec 2009 | info:doi/10.1371/journal.pone.0008083

Gemini, a Bifunctional Enzymatic and Fluorescent Reporter of Gene Expression

Lance Martin, Austin Che, Drew Endy

PLoS ONE:
Published 04 Nov 2009 | info:doi/10.1371/journal.pone.0007569

A Man-Made ATP-Binding Protein Evolved Independent of Nature Causes Abnormal Growth in Bacterial Cells

Joshua M. Stomel, James W. Wilson, Megan A. León, Phillip Stafford, John C. Chaput

PLoS ONE:
Published 08 Oct 2009 | info:doi/10.1371/journal.pone.0007385

A Modified Consumer Inkjet for Spatiotemporal Control of Gene Expression

Daniel J. Cohen, Roberto C. Morfino, Michel M. Maharbiz

PLoS ONE:
Published 18 Sep 2009 | info:doi/10.1371/journal.pone.0007086

Design Parameters to Control Synthetic Gene Expression in Escherichia coli

Mark Welch, Sridhar Govindarajan, Jon E. Ness, Alan Villalobos, Austin Gurney, Jeremy Minshull, Claes Gustafsson

PLoS ONE:
Published 14 Sep 2009 | info:doi/10.1371/journal.pone.0007002

Synthetic Morphology Using Alternative Inputs

Hiromasa Tanaka, Tau-Mu Yi

PLoS ONE:
Published 10 Sep 2009 | info:doi/10.1371/journal.pone.0006946

Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways

Jiayuan Quan, Jingdong Tian

PLoS ONE:
Published 30 Jul 2009 | info:doi/10.1371/journal.pone.0006441

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

Carola Engler, Ramona Gruetzner, Romy Kandzia, Sylvestre Marillonnet

PLoS ONE:
Published 14 May 2009 | info:doi/10.1371/journal.pone.0005553

Monomeric Bistability and the Role of Autoloops in Gene Regulation

Stefanie Widder, Javier Macía, Ricard Solé

PLoS ONE:
Published 30 Apr 2009 | info:doi/10.1371/journal.pone.0005399

Timing Cellular Decision Making Under Noise via Cell–Cell Communication

Aneta Koseska, Alexey Zaikin, Jürgen Kurths, Jordi García-Ojalvo

PLoS ONE:
Published 13 Mar 2009 | info:doi/10.1371/journal.pone.0004872

High-Level Production of Amorpha-4,11-Diene, a Precursor of the Antimalarial Agent Artemisinin, in Escherichia coli

Hiroko Tsuruta, Christopher J. Paddon, Diana Eng, Jacob R. Lenihan, Tizita Horning, Larry C. Anthony, Rika Regentin, Jay D. Keasling, Neil S. Renninger, Jack D. Newman

PLoS ONE:
Published 16 Feb 2009 | info:doi/10.1371/journal.pone.0004489

Design and Construction of a Double Inversion Recombination Switch for Heritable Sequential Genetic Memory

Timothy S. Ham, Sung K. Lee, Jay D. Keasling, Adam P. Arkin

PLoS ONE:
Published 30 Jul 2008 | info:doi/10.1371/journal.pone.0002815

Targeted Development of Registries of Biological Parts

Jean Peccoud, Megan F. Blauvelt, Yizhi Cai, Kristal L. Cooper, Oswald Crasta, Emily C. DeLalla, Clive Evans, Otto Folkerts, Blair M. Lyons, Shrinivasrao P. Mane, Rebecca Shelton, Matthew A. Sweede, Sally A. Waldon

PLoS ONE:
Published 16 Jul 2008 | info:doi/10.1371/journal.pone.0002671

Bimodal and Hysteretic Expression in Mammalian Cells from a Synthetic Gene Circuit

Tobias May, Lee Eccleston, Sabrina Herrmann, Hansjörg Hauser, Jorge Goncalves, Dagmar Wirth

PLoS ONE:
Published 04 Jun 2008 | info:doi/10.1371/journal.pone.0002372

Synthetic Biology of Proteins: Tuning GFPs Folding and Stability with Fluoroproline

Thomas Steiner, Petra Hess, Jae Hyun Bae, Birgit Wiltschi, Luis Moroder, Nediljko Budisa

PLoS ONE:
Published 27 Feb 2008 | info:doi/10.1371/journal.pone.0001680

Reconstitution of Mdm2-Dependent Post-Translational Modifications of p53 in Yeast

Barbara Di Ventura, Charlotta Funaya, Claude Antony, Michael Knop, Luis Serrano

PLoS ONE:
Published 30 Jan 2008 | info:doi/10.1371/journal.pone.0001507

A Microfluidic Device for Temporally Controlled Gene Expression and Long-Term Fluorescent Imaging in Unperturbed Dividing Yeast Cells

Gilles Charvin, Frederick R. Cross, Eric D. Siggia

PLoS ONE:
Published 23 Jan 2008 | info:doi/10.1371/journal.pone.0001468

Printing Multistrain Bacterial Patterns with a Piezoelectric Inkjet Printer

Jack Merrin, Stanislas Leibler, John S. Chuang

PLoS ONE:
Published 25 Jul 2007 | info:doi/10.1371/journal.pone.0000663

High-Yield Hydrogen Production from Starch and Water by a Synthetic Enzymatic Pathway

Y.-H. Percival Zhang, Barbara R. Evans, Jonathan R. Mielenz, Robert C. Hopkins, Michael W.W. Adams

PLoS ONE:
Published 23 May 2007 | info:doi/10.1371/journal.pone.0000456

Designer Self-Assembling Peptide Nanofiber Scaffolds for Adult Mouse Neural Stem Cell 3-Dimensional Cultures

Fabrizio Gelain, Daniele Bottai, Angleo Vescovi, Shuguang Zhang

PLoS ONE:
Published 27 Dec 2006 | info:doi/10.1371/journal.pone.0000119

Adaptive Response of a Gene Network to Environmental Changes by Fitness-Induced Attractor Selection

Akiko Kashiwagi, Itaru Urabe, Kunihiko Kaneko, Tetsuya Yomo

PLoS ONE:
Published 20 Dec 2006 | info:doi/10.1371/journal.pone.0000049

SOURCE

Read Full Post »