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Posts Tagged ‘Nuclease’


Zinc-Finger Nucleases (ZFNs) and Transcription Activator–Like Effector Nucleases (TALENs)

Reporter: Larry H Bernstein, MD, FCAP

 

TALENs and ZFNs are associated with different mutation signatures

Y Kim,  J Kweon  & Jin-Soo Kim

Zinc-finger nucleases (ZFNs) and transcription activator–like effector nucleases (TALENs) are of great interest for genome engineering in higher eukaryotic cells and organisms. These enzymes

  1. contain the same FokI nuclease domain and
  2. induce site-specific DNA cleavage.

http://www.nature.com/nmeth/journal/v10/n3/extref/nmeth.2364-S1.pdf

http://www.nature.com/nmeth/journal/v10/n3/full/nmeth.2364.html?WT.ec_id=NMETH-201303

English: Bacterial multi-drug resistance syste...

English: Bacterial multi-drug resistance system: complex of dimeric transcription-activator protein BmrR with bound TPP, untwisting the DNA to position the two promoter sites (top) for transcription. Coordinates from PDB file 1R8E, Brennan lab; displayed in KiNG. (Photo credit: Wikipedia)

                                   

English: Diagram of a typical rAAV vector

English: Diagram of a typical rAAV vector (Photo credit: Wikipedia)

Splicing activation

Splicing activation (Photo credit: Allen Gathman)

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