Zinc-Finger Nucleases (ZFNs) and Transcription Activator–Like Effector Nucleases (TALENs)
Reporter: Larry H Bernstein, MD, FCAP
TALENs and ZFNs are associated with different mutation signatures
Y Kim, J Kweon & Jin-Soo Kim
Zinc-finger nucleases (ZFNs) and transcription activator–like effector nucleases (TALENs) are of great interest for genome engineering in higher eukaryotic cells and organisms. These enzymes
- contain the same FokI nuclease domain and
- induce site-specific DNA cleavage.
http://www.nature.com/nmeth/journal/v10/n3/extref/nmeth.2364-S1.pdf
http://www.nature.com/nmeth/journal/v10/n3/full/nmeth.2364.html?WT.ec_id=NMETH-201303
English: Bacterial multi-drug resistance system: complex of dimeric transcription-activator protein BmrR with bound TPP, untwisting the DNA to position the two promoter sites (top) for transcription. Coordinates from PDB file 1R8E, Brennan lab; displayed in KiNG. (Photo credit: Wikipedia)
English: Diagram of a typical rAAV vector (Photo credit: Wikipedia)
Splicing activation (Photo credit: Allen Gathman)
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