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Posts Tagged ‘Mitochondrial permeability transition pore’


Calcium Signaling, Cardiac Mitochondria and Metabolic Syndrome

Larry H Bernstein: Author 

and

Reporter: Aviva Lev-Ari, PhD, RN

Mitochondria, the cardiovascular system and metabolic syndrome

Start date
April 24, 2013
End date
April 24, 2013
Venue
London, UK / Kennedy Lecture Theatre, Institute of Child Health
Location
London, UK
Topics
– Mitochondrial ROS metabolism in the heart
– Mitochondrial permeability transition pore
– Mitochondria in vascular smooth muscle
– Therapeutic targets for cardiac disease

Invited speakers

This event has now passed – please visit our Conference calendar for future Abcam events

Confirmed speakers:

Paolo Bernardi, University of Padova, Italy
‘The mitochondrial permeability transition pore: A mystery solved?’

Susan Chalmers, University of Strathclyde Glasgow
‘Mitochondria in vascular smooth muscle: from regulation of calcium signals to control of proliferation’

Andrew Hall, UCL
‘The role of sirtuin 3 in cardiac dysfunction’

Derek Hausenloy, UCL
‘Mitochondrial dynamics as a therapeutic target for cardiac disease’

Guy Rutter, Imperial College London
‘Mitochondria and insulin secretion – links to diabetes’ 

Michael Murphy, MRC Mitochondiral Biology Unit, Cambridge
‘Exploring mitochondrial ROS metabolism in the heart using targeted probes and bioactive molecules’

Toni Vidal Puig, Institute of Metabolic Science, University of Cambridge
‘Adipose tissue expandability, lipotoxicity and the metabolic syndrome’ 

 SOURCE

http://www.abcam.com/index.html?pageconfig=resource&rid=15722

It all happens in a heartbeat

Calcium signaling is instrumental for excitation-contraction coupling (ECC). The involvement of mitochondria  in establishing rapid cytosolic calcium transients in this process remain debated.

Two models have emerged:

  • slow integration versus rapid and
  • ample beat-to-beat changes of

cytosolic calcium transients into the mitochondria matrix.

a brief outline of cardiac calcium signaling » 

Mitochondrial Calcium transport mechanisms 

Calcium influx can be mediated by:

  • Mitochondrial Calcium Uniporter (MCU)
  • Mitochondrial Ryanodine receptor type 1 (mRyR1)
  • Leucine-zipper-EF-hand-containing transmembrane protein 1 (LETM1)
  • Proposed uptake by UCP2 and 3 and Coenzyme Q10

Calcium efflux can be mediated by:

  • Na-dependent calcium extrusion pathway, mNCX1
  • Mitochondrial permeability transistion pore (mPTP)

Inhibiting Calcium signaling 

Homeostasis of mitochondrial Ca2+ is crucial for balancing cell survival, death and energy production. Inhibitors of mitochondrial Ca2+ exchange are:

  1. CGP37157 – Selective mitochondrial Na+-Ca2+ exchange inhibitor
  2. Thapsigargin – Potent, cell-permeable Ca2+-ATPase inhibitor
  3. Ryanodine – Ca2+ release modulator

calcium signaling inhibitors (now available from Abcam Biochemicals)  » 

Quick tools for calcium detection 

You can now detect intracellular calcium mobilization directly in cultured cells in only 1 hour with Fluo-8 No Wash Calcium Assay Kit (ab112129):

  • increased signal with Fluo-8 – high affinity indicator (Kd = 389 nM)
  • no wash step needed
  • works on adherent and suspension cells

The Mitochondria, cardiovascular system and metabolic syndrome meeting took place on April 24 2013,  London, UK.

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