Irina S. Robu, PhD, Material Scientist and Business Developer for Materials Segment of Manufacturing Industry
- Business Developer for RegenHU in the Tissue Engineering Industry in the US Mid West and Canada
- Business Developer for planned Global Distributorship of 3D Printing and related BioMedical Technologies, DBA, LM-3DP-GD based in the US, for the US Mid West and Canada
- Expert, Author, Writer in the following Research Categories
- Stem Cells for Regenerative Medicine
- Bone Disease and Musculoskeletal Disease
- Medical Devices: Stents and Tools
- Technology Transfer: Biotech and Pharmaceutical
- Nanotechnology for Drug Delivery
- Massachusetts Niche Suppliers and National Leaders
Irina Robu, PhD
3D Medical BioPrinting Technology Reporting by Irina Robu, PhD – a forthcoming Article in “Medical 3D BioPrinting – The Revolution in Medicine, Technologies for Patient-centered Medicine: From R&D in Biologics to New Medical Devices”
List of Posts on 3D BioPrinting in the Open Access Online Scientific Journal
Curator: Aviva Lev-Ari, PhD, RN
List of Posts on other Topics on the Open Access Online Scientific Journal
Artificially intelligent synthetic skin interacts with brain cells.
Global 3D Bioprinting Market: Industry Size, Share and Segments Analysis to 2015 – 2021
Mass-producing stem cells to satisfy the demands of regenerative medicine
Newly discovered cells regenerate liver tissue without forming tumors
Pennsylvania Supreme Court reverses Zimmer win in $20m knee implant lawsuit
Rat Hearts Healed by a Protein-rich Gel
Irina Robu, PhD
PhD Chemical Engineering
Scientist with ability to independently lead scientific research and collaborate on a team to achieve scientific goals; develop methods and complete protocol design; execute and troubleshoot experiments; and reporting of data in both written and oral reports. I am currently looking for a research oriented position that provides the ability to utilize my skills, knowledge and experience.
Ph.D. in Chemical Engineering (Biomaterials and Tissue Engineering Research)
Wayne State University, Detroit, MI – May 2011
M.Sc. in Chemical Engineering (Laser Optics Research)
Wayne State University, Detroit, MI – Dec 2004
B.Sc. in Chemical Engineering
Wayne State University, Detroit, MI – May 2003
B.Sc. in Chemistry
University of Windsor, Windsor, Ontario, CA – June 2000
- Strong knowledge of process optimization using statistical design of experiments (DOE), data analysis using excel templates, macros and statistical analysis.
- Familiar with GLP and GMP practices and FDA regulations.
- Hands on experience with mammalian and stem cell culture/expansion, cell banking, cell culture in 2D and 3D culture systems, single use bioreactors (batch and perfusion), metabolite analysis, cell based assays (proliferation/cytotoxicity assays), analytical protein assays (ELISA, Western Blot), PCR, immunohistochemistry and cell imaging (optical, fluorescence and SEM).
- Proficient with various techniques such as cell based assays, histology, chromatography (HPLC, GC), microscopy (optical, fluorescence, SEM), spectroscopy (UV-Vis, FTIR), wet chemistry (pH, conductivity, titrations), immunostaining and SDS-Page.
- Solid laboratory experience which includes statistically analyzing data, effectively identifying ways to optimize
experiments and prepared quantitative/qualitative analysis of the data.
- Ability to plan, prioritize and execute experiments in order to effectively meet project goals and timelines.
- Strong technical ability in maintaining records, technical reports and protocols, technical writing, reporting data and communicating scientific work in the form of presentations and posters.
- Advanced proficiency with MS Office Suite (Excel, Word, Power Point and Outlook) and image analysis software (Image J, Adobe Photoshop).
Bioengineering Perfusion bioreactor design, development and culture for 3-D constructs, Scaffold fabrication and culture (cell surface properties, seeding efficiency and biomaterial/cell culture), Biomaterial characterization (Stress-strain, biocompatibility, ultimate strength, cell attachment), Scaffold manufacture: Freeze drying, Electrospinning.
Cell Culture Isolation and culture of primary (SMC, EC) and stem cell lines (Endometrial Cells, Human and rat MSC), Cell Banking, Media preparation and Cryopreservation.
Instrumentation Microscopy (optical, fluorescence, SEM), FTIR, UV Spectrophotometer, Fluorometer, X-ray Diffraction (XRD), Tensile Testing Instruments (Instron, MTS), Freeze dryer, Refractometer, Viscometer, Evaporator, X-ray micro tomography and Microtome.
Molecular Techniques ELISA, HPLC, RT-PCR, DNA isolation, RNA isolation, Western blot, Gel electrophoresis.
Biochemical Assays Cell viability assays (Fluorescent: Alamar Blue, CyQuant; Colorimetric: MTT, XTT), Immunohistochemistry, Cell counting assays, Stem cell staining assays and differentiation assays, StemTAG alkaline phosphatase assay, Material implantation and extraction and Histology.
Computer Skills Sigma Scan Pro 5, Sigma Plot 2001,Origin 8, JMP 11, Image J, Endnote X2, Adobe Photoshop, Microsoft Office Package, Math Cad 2001, HYSIS, Minitab 14, SciFinder Scholar, Laboratory Information Management System (LIMS).
Expert Author/Writer for Leaders in Pharmaceutical Business Intelligence (Home Based), 2014-Present
Curated, authored, edited articles and regulation documents on the following Research Categories:
- Nanotechnology for Drug Delivery
- Cell Therapy
- Technology Transfer: Biotech and Pharmaceutical
- Current responsibilities include establishing the IP position and developing the R&D program focused on the innovation of novel nano-oncolytic-immunotherapeutic technologies for cancer patients with high unmet medical need.
Patent Researcher, Landon IP, Southfield, Michigan, 2012-2013
- Performed qualitative analysis of patents and patent portfolios from, scientific, technical and business perspective.
- Analyzed biomedical/chemical and biomedical device related databases (NCBI, PUB Med), IEEE journals, IEEE research papers, dissertations, thesis and other technical documentation for relevant prior art.
Postdoctoral Researcher, Indiana University-Purdue University, Indianapolis, IN, 2011-2012
- Reviewed recent literature in the field and produce novel ideas for project improvement.
- Compiled and presented results at the internal meetings with collaborators.
- Conducted independent research to examine the osteogenic differentiation potential of mesenchymal stem cell and endometrial stem cells to test its feasibility for bone tissue engineering applications using analysis such as flow cytometry, RT-PCR, histological analysis, immunofluorescence, SEM, cell based assays for proliferation and enzyme based assays.
- Conducted implant studies to check the effects of 3D cylindrical SLS scaffolds on bone formation in rats.
- Analyzed the 3D cylindrical bio-glass scaffolds pre-implantation using a) FTIR, XRD and SEM to assess their chemical bonding, composition and surface morphology of the scaffold and using MTS to assess their mechanical stability and post implantation a) histology to assess the presence of mineralized calluses bridging the gap of the segmental bone defects and b) verified the presence of tissue using micro-CT.
- Evaluated post implant bioactive glass scaffold for bone resorption, mineral deposition, mechanical stability and tissue formation around the implant.
- Development of a bone tissue engineering strategy based on combination of mesenchymal stem cells and bioglass scaffolds.
- Manage three projects simultaneously; prepare complex data findings, and present data to collaborators from several therapeutic areas, including Hematology/Oncology, Dental Materials and Biomedical Engineering.
Graduate Research Assistant, Chemical Engineering, Wayne State University, Detroit, MI, 2005–2011
- Managed daily operations of the lab including stock inventory, calibration/maintenance of equipment, training new lab members and mentored several undergraduate students.
- Performed tissue cultures, media changes, and subculture of adherent mammalian cell lines harvested from pig aorta.
- Investigated the effects of glycosaminoglycan (GAG) and GAG-like polyanion as modifiers for smooth muscle and endothelial cells activity by evaluating the effects of heparin, dextran sulfate immobilized on chitosan and chitosan-collagen films on morphology and proliferation of aortic cells.
- Conducted cell adhesion and proliferation studies on chitosan/collagen/GAG 3D matrices by modifying growth factor concentration within the medium composition.
- Studied and assessed cell phenotype, migration, viability, cytoskeleton organization of SMC and EC cells seeded on chitosan/GAG and chitosan/collagen/GAG films and 3D chitosan/collagen scaffolds.
- Prepared composite (combination of non-porous and porous) and three-dimensional porous scaffolds for tissue engineered implants applications and characterized the scaffolds using mechanical and morphological methodologies to assess their pore size, pore geometry, interconnectivity and their efficiency for bioreactor culture.
- Evaluated the cell proliferation, viability, biocompatibility and cell matrix content on seeded vascular graft in static and dynamic 3D cultures using various biochemical assays.
- Conducted cell-biomaterial interaction studies in vitro using a continuous perfusion bioreactor cell culture system by controlling the critical parameters such as medium composition, flow and scaffold seeding.
- Set up and maintained small-scale bioreactor cell culture runs.
- Studied the effects of variable flow and medium composition (via perfusion bioreactor) on viability, morphology and metabolites.
Collaborative Research Projects
- Collaborated on studying mechanical and cell interaction properties on sebacate-chitosan cross-linked membranes
- Evaluate mechanical properties – tensile strength, elastic modulus and breaking strain of the cross-linked and non-cross-linked materials.
- Coordinated and conducted all aspects of cell culture studies on immobilized glycosaminoglycan chitosan/collagen surfaces and vascular graft culture in a 3D bioreactor system research including study design, protocol writing, statistics, data analysis, report writing, presentation and publications.
Graduate Research Assistant, Chemical Engineering Department, Wayne State University, Detroit, MI, 2003-2004
- Prepared and evaluated large density gradient experiments with compressible supercritical CO2 and calibrated single ray path inhomogeneous supercritical CO2.
- Quantitative measurements in a continuum of densities, solute concentrations and temperatures.
- We were able to show that small temperature gradient within a cell contains the fluid resulted large density gradients that are almost proportional to gradients of optical refractive index. Results were published results in Journal of Flow and Visualization.
Research Engineer, Detroit Edison -Warren Service Center, Detroit MI, 2001–2003
- Performed routine experiments, analysis and calculations for internal reports.
- Responsible for the general lab support activities such as equipment maintenance and calibration.
- Refined oil sampling technique and analysis with reference sampling to provide more accurate results.
- Investigated and performed new procedures for determining CaO and NH3 in fly ash used.
- Compiled and wrote a technical training manual for the Laboratory Information Management System (LIMS) program.
Graduate Teaching Assistant, Chemical Engineering, Wayne State University, Detroit, MI, 2007-2008; 2009-2010
- Offered assistance with projects, homework, design project, HYSIS term project.
- Presented fundamental concepts and test strategies, graded student assignment and exams for Thermodynamics (CHE 3300) and Transport Phenomena (CHE 3200), Product and Process Design (CHE 4200) and Process Dynamics and Simulation (CHE 4600), Chemical process Integration (CHE 4800) and Material and Mass Balances (CHE 2800).
Research Assistant, University of Windsor, Chemistry, Windsor, Ontario, Canada, 1998–2000
- Synthesized and characterize zirconium complexes using infrared spectroscopy, H-NMR and C-NMR and X-ray crystallography.
- Robu I.S., Chu Tien-Min Gabriel., In Vivo Evaluation of Bioactive Glass Scaffolds through Selective Laser Sintering, World Biomaterials Congress 2012, poster #3006.
- Robu I.S., Walters H.L., Matthew H.W.T.,Collagen Gel Effects on Smooth Muscle Cell Seeding Efficiency and Function in Chitosan Scaffolds, American Institute of Chemical Engineering 2009, Nashville, Tennessee; Oral Presentation in Biomaterial Scaffolds for Tissue Engineering.
- Robu I.S., Walters H.L., Matthew H.W.T.,Collagen Gel Effects on Smooth Muscle Cell Seeding Efficiency and Function in Chitosan Scaffolds, Biomedical Engineering Society 2009, Pittsburg, Pennsylvania; Poster Presentation in Biomaterials for Tissue Engineering #2332.
- Robu I.S., Walters H.L., Matthew H.W.T., Development of a Chitosan Fiber-Reinforced, Large Vessel Graft for Pediatric Applications, Tissue Engineering & Regenerative Medicine International Society (TERMIS) North America 2008 Conference and Exposition, San Diego, California, poster # 622.
- Robu I.S., Walters H.L., Matthew H.W.T., A Fiber-Reinforced, Large Vessel Chitosan Scaffold for Pediatric Applications, American Institute of Chemical Engineers 2008, 30, Oral Presentation in Biomaterial Scaffolds for Tissue Engineering Section, 396f.
- Robu I.S., Walters H.L., Matthew H.W.T., Morphological and Growth Responses of Vascular Smooth Muscle Cells and Aortic Endothelial Cells Cultured on Immobilized Heparin and Dextran Sulfate Surfaces, TERMIS North America 2007 Conference and Exposition, Toronto, Canada, poster #1495.
- Robu I.S., Rothe Erhard W., Probes of Supercritical CΟ2 by Deflection of Multiple Beamlets; American Chemical Engineering Society Annual Meeting 2006, oral presentation.
- Liu Wai Ching, Robu Irina, Patel Rikin, Leu Ming, Velez, Mariano, Chu, Tien-Min, The effects of 3D bioactive glass scaffolds and BMP-2 on bone formation in rat femoral critical size defects and adjacent bones, Biomedical Materials, Vol.9, Nr. 4, 2014 (* 50% contribution)
- Robu I.S., Walters III Henry L., Matthew Howard W.T. Morphological and Growth Responses of Vascular Smooth Muscle and Endothelial Cells Cultured on Immobilized Heparin and Dextran Sulphate Surfaces, Manuscript under review at Journal of Biomaterials Research: Part A, 2016.
- Robu I.S., Walters H.L., Matthew H.W.T. Morphological and Growth Responses of Vascular Smooth Muscle Cells Cultured on Immobilized Heparin and Dextran Sulfate Surfaces; Transactions of the 32nd Annual Meeting of the Society for Biomaterials 2007, 30, p646.
- Robu I.S. and Erhard Rothe, Probes of supercritical CO2 by deflection of multiple beamlets, Journal of Flow Visualization & Image Processing, Vol. 12, pp. 1–11, 2005.
- Piparia R., Robu I.S, Matthew H.W.T. Optimizing Mechanical and Cell Adhesion Properties of Chitosan through Simultaneous Manipulation of Molecular Weight and Cross linking. Transactions of the 31st Annual Meeting of the Society for Biomaterials, 2006, 29: p278.
- Wayne State- Department of Chemical Engineering Teaching Assistantship (2007-2010)
- Biomedical Engineering Society Travel Award Recipient, Pittsburgh (2009)
- Perry Foundation Research Assistantship via Detroit Children’s Hospital (2005-2008)
- Tissue Engineering and Regenerative Medicine Society Travel Award, Toronto (2007)
- Graduate Research Fellowship, Industrial Manufacturing Research (2003-2005)