New Spinal Cord Repair Strategy using 3D Cell Growth
Reported by: Irina Robu, PhD
The study published in the Nature group journal Scientific Reports writes that using floating liquid marbles cells are are able to freely associate and form natural structures as they would do in the normal body. Dr. James St John, PhD from Griffith’s Eskitis Institute for Drug Discovery says “Allowing cells to grow in this 3D format dramatically increases their growth and function and is particularly useful for spinal transplantation repair in which cells are transplanted into the injury site”.Dr. St John explains that a specialized cell type is taken from the olfactory (sense of smell) system.
“A droplet of liquid that contains the cells is placed upon a carpet of teflon powder to create a liquid marble which can then be floated on cell culture medium,” he notes in the release.
“By having an air interface between the liquid marble and the cell culture medium upon which it floats, the liquid marble easily rotates. This allows the cells within the liquid marbles to freely associate to form natural structures without the confines imposed upon them by other 3D culturing methods,” he adds.
The new method enables transplanted cells to survive and better integrate into the injury site, possibly helping the spinal cord to regenerate more effectively.
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This is very insightful. There is no doubt that there is the bias you refer to. 42 years ago, when I was postdocing in biochemistry/enzymology before completing my residency in pathology, I knew that there were very influential mambers of the faculty, who also had large programs, and attracted exceptional students. My mentor, it was said (although he was a great writer), could draft a project on toilet paper and call the NIH. It can’t be true, but it was a time in our history preceding a great explosion. It is bizarre for me to read now about eNOS and iNOS, and about CaMKII-á, â, ã, ä – isoenzymes. They were overlooked during the search for the genome, so intermediary metabolism took a back seat. But the work on protein conformation, and on the mechanism of action of enzymes and ligand and coenzyme was just out there, and became more important with the research on signaling pathways. The work on the mechanism of pyridine nucleotide isoenzymes preceded the work by Burton Sobel on the MB isoenzyme in heart. The Vietnam War cut into the funding, and it has actually declined linearly since.
A few years later, I was an Associate Professor at a new Medical School and I submitted a proposal that was reviewed by the Chairman of Pharmacology, who was a former Director of NSF. He thought it was good enough. I was a pathologist and it went to a Biochemistry Review Committee. It was approved, but not funded. The verdict was that I would not be able to carry out the studies needed, and they would have approached it differently. A thousand young investigators are out there now with similar letters. I was told that the Department Chairmen have to build up their faculty. It’s harder now than then. So I filed for and received 3 patents based on my work at the suggestion of my brother-in-law. When I took it to Boehringer-Mannheim, they were actually clueless.