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Gene Silencing, Volume 2 (Volume Two: Latest in Genomics Methodologies for Therapeutics: Gene Editing, NGS and BioInformatics, Simulations and the Genome Ontology), Part 2: CRISPR for Gene Editing and DNA Repair
The scientists fused Cas9 to zinc finger proteins to reduce off-target activity, essentially replacing the other nucleases that had been used with zinc fingers.
A study in Molecular Cellshows CRISPR-Cas9-like genome editing in human cells and suggests one protein could be an RNA-guided, RNA-cleaving endonuclease.
The work could potentially make lab-based blood production cost-effective, as well as improve the creation of specific cell populations from stem cells.
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