Mini-kidney organoids re-create disease in lab dishes
Reporter: Irina Robu, PhD
Kidney disease affects about 700 million people worldwide and the costs are tremendous. Dialysis and kidney transplantation are the only options of kidney failure which can cause harmful side effects and poor quality-of-life.
To re-create human disease, Freedman and his colleagues used the gene-editing technique called CRISPR. They engineered mini-kidneys with genetic changes linked to two common kidney diseases, polycystic kidney disease and glomerulonephritis. The mini-kidney organoids are grown using genome editing to recreate human kidney disease in petri dishes. The achievement is published on Nature Communications, today October 23 and it paves the way for personalized drug discovery for kidney disease.
Pluripotent stem cells are used to grow the mini-kidney organoids. When treated with a chemical cocktail, the stem cell matured into structures that resemble miniature kidneys. The organoids contain filtering cells, blood vessel cells and tubules and developed characteristics of these diseases. Those with mutations in polycystic kidney disease genes formed balloon like, fluid filled sacks, called cysts, from kidney tubules. The organoids with mutations in podocalyxin, a gene linked to glomerulonephritis, lost connections between filtering cells.
Source
2012pharmaceutical
Amandeep Kaur
Aashir Awan, Phd
Abhisar Anand
Adina Hazan
Alan F. Kaul, PharmD., MS, MBA, FCCP
alexcrystal6
anamikasarkar
apreconasia
aptubman
Arav Gandhi
aviralvatsa
David Orchard-Webb, PhD
danutdaagmailcom
Demet Sag, Ph.D., CRA, GCP
Dror Nir
dsmolyar
Ethan Coomber
evelinacohn
FrasonKalapurackal
Gail S Thornton
Irina Robu
jayzmit48
jdpmd
jshertok
kellyperlman
Ed Kislauskis
larryhbern
Madison Davis
marzankhan
megbaker58
ofermar2020
Dr. Pati
pkandala
ritusaxena
Rick Mandahl
sjwilliamspa
Srinivas Sriram
stuartlpbi
Dr. Sudipta Saha
tildabarliya
zraviv06
zs22
Leave a Reply