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Microgravity Expanded Stem Cells

Posted in Cerebrovascular and Neurodegenerative Diseases, Clinical & Translational, Curation, Disease Biology, Neurological Diseases, Neuroscience, Regenerative Biology and Medicine, Stem Cells for Regenerative Medicine, tagged , on November 13, 2015| Leave a Comment »

Microgravity Expanded Stem Cells

Larry H. Bernstein, MD, FCAP, Curtor

LPBI

 

 

Application of Microgravity Expanded Stem Cells in Regenerative Medicine

Author: Abba C Zubair MD PhD   Mayo Clinic      http://medical.wesrch.com/pdfME1LYYYIWQLGL

Description: Application of Microgravity Expanded Stem Cells in Regenerative Medicine:- Regrettably I have no financial disclosures, However, I am available if any company is interested. MSCs for Hemorrhagic Stroke.Oxygen-Glucose Deprivation Model (OGD).Effect of MSC on OGD-Stressed Neurons. Mechanism of MSC induced neuro-recovery.Mechanism of MSC induced immune regulation.

ICH model:- Autologous blood withdraw from femoral Artery MSCs or medium infusion via femoral vein With the help of stereotactic frame and microinjection pump autologous blood will be injected to right baal ganglia, 10µl/min total 100µl.Brain sections @D1 after ICH:- 100ul autologous blood Right basal ganglion Coordinates: 0.2 mm anterior, 5.5mm ventral, and 3.5 mm lateral to bregma. Arrow shows hematoma initiated location.Behavior test includes Forelimb Use Asymmetry test and Rotometer test. Behavior (Forelimb Use Asymmetry Test)- Media, MSC infusion (1×10^6/kg).

Stem Cells in Microgravity- No effective method to expand human stem cells, Need about 200 to 500 million stem cells to achieve effective therapy.Objectives- Measure cell proliferation in microgravity versus gravity of Earth, Observe cell growth and morphological characteristics in microgravity, Analyze gene expression profiles of cells grown in microgravity, Use viable cells grown in microgravity in a variety of downstream in vitro and in vivo experiments on Earth.BioCell Cassettes:- Created by BioServe Space -Aerospace Engineering and Sciences Department at …Please navigate Paper pages for more details

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