Roles at http://pharmaceuticalintelligence.com

Research Category OWNER:

• Bio Instrumentation in Experimental Life Sciences Research
• Reproductive Biology & Bio Instrumentation

Updated on 2/10/2020

Dr. Sudipta Saha

Assistant Professor (III),

Amity Institute of Physiology and Allied Sciences (AIPAS),

Amity University, Noida, Uttar Pradesh,

Sector-125, Noida – 201313

Mobile: +91 9830079019, +91 9810349961

E-Mail: sudiptasaha49@yahoo.co.in, sudiptasaha1977@gmail.com, ssaha2@amity.edu

Permanent Residence Address:

Kolkata, West Bengal, India.

BIO

Research interests are

• Reproductive Biology (Specifically sperm motility) and
• Cell Biology (Specifically cell cycle regulation).

Education

• Post Doc from Chang Gung University, Taiwan
• PhD in Life Science from CSIR-Indian Institute of Chemical Biology (IICB) / Jadavpur University
• BSc and MSc in Physiology from Calcutta University

Appointments

• Assistant Professor (III) of Physiology at Amity University Noida Extramural Project Grant of Rs. 3 million from DST-SERB (Science and Engineering Research Board-Start-up Research Grant).
• Author/Reporter/Curator for pharmaceuticalintelligence.com LPBI Group, Boston since 2012.
• Pool Scientist at CSIR-IICB

Publications

• 14 Journal Publications,
• 4 Book Chapters,
• 7 e-Books,
• 1 Granted Patent,
• 5 Press Mentions

Awards

Recipient of 4 Awards during PhD.

 

An enthusiastic and skilled researcher in Reproductive Biology, Physiology, Cell Biology, Molecular Biology and Biochemistry.

 

Research Interest:

• Cell Biology, Molecular Biology and Physiology
• Reproductive Biology and Protein Biochemistry
• Bio-Instrumentation and Application Oriented Research

Professional Training:

• Assistant Professor (III), Amity Institute of Physiology and Allied Sciences, Amity University, Noida, UP.

June 2017 – Present

• Senior Research Associate (CSIR Scientists’ Pool Scheme / Pool Officer), IICB, Kolkata, India.

November 2015 – June 2017

• Post Doctoral Research Associate (NSC), Chang Gung University, Taiwan.

November 2012 – April 2015

• Scientific Author, Curator & Reporter, http://www.pharmaceuticalintelligence.com,

July 2012 – Present (Associated without any payment)

• Post Doctoral Research Associate (NSC), Chang Gung Memorial Hospital, Taiwan.

June 2011 – June 2012

• Post Doctoral Research Associate (CSIR), Indian Institute of Chemical Biology (IICB), Kolkata, India.

February 2009 – June 2011

 

Research Techniques Known:

• Mammalian cell (U2OS, HEK293, 293T, 293FT, HeLa, C2C12, 3T3, LNCaP, PC3, etc. & different stable clones) and Bacterial cell culture, Primary cell culture, Mouse embryonic fibroblast culture, Baterial transformation, Plasmid purification, Transfection, Lentiviral transfection, PCR, experiments with gene knockout mice.
• ELISA, Western Blotting, Immunoprecipitation assay, immunofluorescence staining, fluorescent microscopy and FACS.
• Protein purification by different Chromatography, Electrophoresis, Histological slide preparation, etc.
• Animal handling: Laboratory rats, mice, rabbits and some surgery (ectomy), gene knockout mice breeding and experiments.
• Microscopic and spectrophotometric sperm motility assay techniques, General biochemical assays, etc.
• Process and instrument development by collaboration with different people with suitable expertise.

Education:

• D. in Life Science (Ph.D. awarded December 24, 2008)

CSIR-Indian Institute of Chemical Biology, Jadavpur University, Kolkata, India

Thesis Title: “A novel method of sperm motility analysis and characterisation of a sperm motility promoting protein           from goat blood serum”. Advisor: Dr. G. C. Majumder and Dr. S. R. Dungdung, Sperm Biology Laboratory, Cell Biology and Physiology Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.

• Master of Science in Physiology (October 2001)

Presidency College, University of Calcutta, Kolkata, India. (Percentage of Marks: 64.10%)

Project Title: “Effect of oral exposure of arsenic on certain physiological parameters related to ovarian endocrine functions”. Advisor: Prof. Chandan Mitra, Head, Dept. of Physiology, Presidency College, Kolkata, India.

• Bachelor of Science in Physiology (August 1999)

City College, University of Calcutta, India. (Percentage of Marks: 56.75%, Honours Graduation)

With Minors: Zoology, Chemistry (Percentage of Marks: 60% in Pass Graduation)

• Madhyamick (10^th, 1994, 79.44%) and Higher Secondary (10+2, 1996, 63.40%)

St. Lawrence High School, Kolkata, India.

 

Funded Projects:

1. DST-Science and Engineering Research Board (SERB)-Start-up Research Grant (2019): Isolation and characterization of the receptor of sperm motility stimulating protein from sperm membrane and finding its role and mechanism in fertility management. (SERB File Number: SRG/2019/000501, Fund: Rs. 33 Lakhs)

 

Patents:

 

1. Paul, G. C. Majumder,S. Saha, A. Mukherjee, S. Banerjee. A spectrophotometric system for measurement of vertical velocity of clinical and biological samples. Indian Patent Application Filed – No. 1605/DEL/2004, Date: 26.08.2004. Indian Patent Granted: Patent No. IN264556, Date of Grant: 06.01.2015, International Classification: G01N 21/25. International PCT Patent Application Filed – PCT/IB05/02541.

 

Journal Papers:

1. Bhoumik A., Saha S., Payghan P. V., Ghosh P., Dungdung S. R. (2018): Localization of MIF-II on mammalian spermatozoa: A study revealing its structure, function and motility inhibitory pathway. International Journal of Biological Macromolecules, 116, 633-647.
2. Ghosh P., Bhoumik A., Saha S., Mukherjee S., Azmi S., Ghosh J. K., Dungdung S. R. (2017): Spermicidal efficacy of VRP, a synthetic cationic antimicrobial peptide, inducing apoptosis and membrane disruption. Journal of Cellular Physiology, 233(2), 1041-1050.
3. Pan T. L., Hsu S. Y., Wang P. W., Cheng Y. T., Chang Y. C., Saha S., Hu J., and Ouyang P. (2015): FLJ25439, a novel cytokinesis-associate protein, induces tetraploidization and maintains chromosomal stability via enhancing expression of endoplasmic reticulum stress chaeperones. Cell Cycle, 14(8), 1174-1187.
4. Saha S., Dungdung S. R., Majumder G. C., (2014): Determination of the antioxidant potential of goat sperm cells. Oxidants and Antioxidants in Medical Science, 3(3), 195-200.
5. Bhoumik A., Saha S., Majumder G. C., Dungdung S. R. (2014): Optimum calcium concentration: a crucial factor in regulating sperm motility in vitro. Cell Biochemistry and Biophysics, 70, 1177-1183.
6. Saha S., Das S., Bhoumik A., Ghosh P., Majumder G. C. and Dungdung S. R. (2013): Identification of a novel sperm motility stimulating protein from caprine serum: its characterization and functional significance. Fertility and Sterility, 100(1), 269-279.

(This paper was selected and listed by “Global Medical Discovery” as a potential bio-molecule for future drug development potential)

7. Pei Y.C., Chang T. Y., Lee T. C., Saha S., Lai H. Y., Gomez-Ramirez M., Chou S. W., Wong A. M. K. (2013): Cross-modal sensory integration of visual-tactile motion information: Instrument design and human psychophysics. Sensors, 13(6), 7212-7223.
8. Das S., Saha S., Majumder G. C. and Dungdung S. R. (2010): Purification and Characterization of a Sperm Motility Inhibiting Factor from Caprine Epididymal Plasma. PLoS ONE, 5(8): e12039 (1-12).
9. Jaiswal B. S., Das K., Saha S., Dungdung S. R., Majumder G. C. (2010): Purification and Characterization of a Motility Initiating Protein from Caprine Epididymal Plasma. Journal of Cellular Physiology, 222, 254-263.
10. Saha S., Paul D., Mukherjee A., Banerjee S., Majumder G. C. (2007): A computerized spectrophotometric instrumental system to determine the “vertical velocity” of sperm cells: a novel concept. Cytometry Part A. 71A, 308-316.

(“The Telegraph” reviewed this paper on 14 May, 2007; “News Online” reviewed this paper on Sept, 2009) (National[1605/DEL/2004], International [PCT/IB05/02541] patent applications filed, Won several awards including “Biotech Idea to Innovation Award 2007” from British Council; “India Innovation Pioneers Chalange 2009 from Indo-US Science and Technology Forum)

11. Mandal M., Saha S., Ghosh A. K., Majumder G. C. (2006): Identification and characterization of a sperm motility promoting glycoprotein from buffalo blood serum. Journal of Cellular Physiology, 209(2), 353-362.

(“The Statesman” reviewed this paper on 10 Jan, 2007; “Down to Earth” reviewed this paper on 15 Jan, 2007.)

12. Saha S., Paul D., Mukherjee A., Banerjee S., Majumder G. C. (2006): Development of a unique computerized spectrophotometric system with vertical movement of the cuvette for sperm motility analysis. In: Proceedings of Canadian Conference on Electrical and Computer Engineering (CCECE/CCGEI, IEEE – Ottawa, May 2006). pp. 183-186. Online Publication pp. 1399-1402, DOI: 10.1109/CCECE.2006.277432.
13. Chattopadhyay D., Dungdung S. R., Das K., Saha S., Mandal A. B., and Majumder G. C. (2005): Sperm motility inhibiting activity of a phytosterol from Alstonia macrophylla Wall ex A. DC. Leaf extract: a Tribal Medicine. Indian Journal of Experimental Biology, 43, 1104-1109.
14. Kundu C. N., Das K., Chakrabarty J., Dutta P., Bhattacharyya D., Saha S., Ghosh A., and Majumder G. C. (2003): Development of a novel epididymal sperm model for evaluating cryoprotecting potential of various reagents. Indian Journal of Cryogenics, 28(3), 79-89.

 

Book Chapters / Review Articles:

 

1. Lev-Ari A., Bernstein L. H., Williams S. J., Saha S., Nir D., Robu I., Thornton G. S. Feldman M. W. (2019): Latest in Genomics Methodologies for Therapeutics: Gene Editing, NGS & BioInformatics, Simulations and the Genome Ontology (Series B Book 2), Kindle Edition. (e-book) https://www.amazon.com/dp/B07MGSFDWR, Editors: Stephen J. Williams, Aviva Lev-Ari, Marcus W. Feldman, http://www.pharmaceuticalintelligence.com.
2. Sarkar A., Saha S. (2019): Diagnosis of Arrhythmias by Translational Bioinformatics: An Artificial Intelligence Approach. Review in Book: Artificial Intelligence – An Inducement of Technology in Human Affairs, Page: 228-248, Editors: Shefali Raizada and Ekta Jha, Wolters Kluwer, Gurgaon, India.
3. Pearlman J. D., Bernstein L. H., Lev-Ari A., Saha S., Sag D., Dragoi D., Lal V. (2018): Pharmacological Agents in Treatment of Cardiovascular Diseases (Series A: Cardiovascular Diseases Book 5), Kindle Edition. (e-book) https://www.amazon.com/dp/B07MGSFDWR, Editors: Justine D. Pearlman, Larry H. Bernstein, Aviva Lev-Ari, http://www.pharmaceuticalintelligence.com.
4. Bernstein L. H., Lev-Ari A., Williams S. J., Nallaseth F., Brook I., Saha S., Sag D., Saxena R., Barliya T., Thornton G. S. (2017): The VOICES of Patients, Hospitals CEOs, Health Care Providers, Caregivers and Families: Personal Experience with Critical Care and Invasive Medical Procedures, Kindle Edition. (e-book) https://www.amazon.com/dp/B076HGB6MZ, Editor: Gail S. Thornton, http://www.pharmaceuticalintelligence.com.
5. Bernstein L. H., Lev-Ari A., Williams S. J., Sag D., Robu I., Barliya T., Orchard-Webb D., Kaul A. F., Dragoi D., Saha S.(2017): The Immune System, Stress Signaling, Infectious Diseases and Therapeutic Implications, Kindle Edition. (e-book) https://www.amazon.com/dp/B075CXHY1B, Editor: Sudipta Saha, http://www.pharmaceuticalintelligence.com.
6. Dungdung S. R., Bhoumik, A., Saha S., Ghosh P., Das K., Mukherjee S., Nath D., Chakrabarty J., Kundu C., Jaiswal B. S., Mandal M., Maiti A., Roychowdhury M., Ray, D., Bhattacharyya D., Majumder G. C. (2016): Identification of Sperm Motility Regulatory Proteins: A Potential Approach towards Animal Reproduction. Review in Book: Insights from Animal Reproduction, Page: 161-177, Editor: Rita Payan Carreira, InTech, Rijeka, Croatia.
7. Pearlman J. D., Saxena R., Saha S., Sag D., Williams S. J., Baker M., Bernstein L. H., Lev-Ari A., Sonnenberg A. (2015): Regenerative and Translational Medicine: The Therapeutic Promise for Cardiovascular Diseases, Kindle Edition. (e-book) http://www.amazon.com/dp/B019UM909A, Editors: Larry H Bernstein, Aviva Lev-Ari, http://www.pharmaceuticalintelligence.com.
8. Pearlman J. D., Williams S. J., Barliya T., Saha S., Stoicescu M. Vatsa A., Lev-Ari A., Bernstein L. H., Sonnenberg A. (2015): Etiologies of Cardiovascular Diseases: Epigenetics, Genetics and Genomics, Kindle Edition. (e-book) http://www.amazon.com/dp/B018PNHJ84, Editors: Aviva Lev-Ari, Larry H Bernstein, http://www.pharmaceuticalintelligence.com.
9. Saha S., Saxena R., Barlyia T., Sarkar A., Feldman M. W., Sag D., Bernstein L. H., Williams S. J., Lev-Ari A., Sonnenberg A. (2015): Genomics Orientations for Personalized Medicine (Frontiers in Genomics Research Book 1) Kindle Edition. (e-book) http://www.amazon.com/dp/B018DHBUO6, Editors: Larry H Bernstein, Aviva Lev-Ari, Stephen J. Williams, http://www.pharmaceuticalintelligence.com.
10. Majumder G. C., Saha S., Das K., Nath D., Maiti A., Dey S., Roy D., Dey CS., Mitra S., Rana A., Chakrabarty J., Das S., Bhoumik A., Banerjee S., Mandal M., Jaiswal B. S., Ghosh P., Das A., Bhattacharyya D., and Dungdung S. R. (2015): Role of Sperm Surface Molecules in Motility Regulation.Review in Book: Mammalian Endocrinology and Male Reproductive Biology, Page: 197-244, Editor: Shiokumar Singh, CRC Press, Taylor & Francis Asia Pacific, New Delhi, India.
11. Bernstein L. H., Kandala P., Lev-Ari A., Saha S., Saxena R., Williams S., Sonnenberg A. (2015): Metabolic Genomics & Pharmaceutics: Volume I (BioMedicine – Metabolomics, Immunology, Infectious Diseases Book 1) Kindle Edition. (e-book) http://www.amazon.com/dp/B012BB0ZF0, Editor: Larry H Bernstein, http://www.pharmaceuticalintelligence.com.
12. Majumder G. C., , Das K., Saha S., Nath D., Maiti A., Das S., Dey N., Banerjee S., Barua M., Mandal M., Jaiswal B. S., Biswas R., Bhoumik A., Roy D., Dey S., Som J., Bhattacharyya D., Dungdung S. R. (2012): Purification and Characterization of Novel Sperm Motility-Related Proteins. (Review) Protein Purification, Page: 1-90 Editors: Miguel Benitez and Victoria Aguirre, Nova Science Publishers Inc., USA.

 

Press Mentions:

 

1. “Global Medical Discovery” listed our paper in 2013 on goat serum motility promoting protein as a potential bio-molecule for future drug discovery.
2. “News Online” reported our work on the sperm vertical velocity measuring instrumental system in their September 2009 issue on 14th September 2009 following “Indo-US Honorable Mention Award”.
3. “The Telegraph” has reviewed our work on the sperm vertical velocity measuring instrumental system in the “Know How” scientific section on May 14, 2007.
4. “Down to Earth” the renowned science and technology magazine has reviewed our work on buffalo serum motility promoting protein on January 15, 2007.
5. “The Statesman” has reviewed our work on buffalo serum motility promoting protein on January 10, 2007.

Awards & Honors:

1. Awarded Best Poster Award, Bangalore India Bio, 2011.
2. Awarded Honorable Mention Award in India Innovation Pioneers Challenge Business Plan Competition, Indo-US Science & Technology Forum, New Delhi, 2009.
3. Finalist in BioAsia Innovation Award, BioAsia, Hyderabad, 2009.
4. Finalist in Ranbaxy Young Scientist Award, Ranbaxy Research Laboratories, Gurgaon, 2008.
5. Awarded Biotech Idea to Innovation Award, British Council, New Delhi, 2007.
6. Awarded Best Poster Award, International Congress On Gamete Biology: Emerging Frontiers In Fertility And Contraceptive Development, National Institute of Immunology, New Delhi, 2006.
7. Awarded National Scholarship for getting Star Marks in Madhyamick Pariksha, National Scholarships Scheme, Govt. of West Bengal, 1994.

Workshops and Research Training:

1. Participation in the “Hands-on Basic Flow Cytometry Course” during 17 – 21 December 2009, by Centre for Cellular and Molecular Platform (C-CAMP) at National Centre for Biological Sciences (NCBS), Bangalore. (Training on Becton Dicson (BD) FACS Aria).
2. Participation in “CSIR 6th Technology Led Entrepreneurship Programme 2009” during 1 – 26 June 2009, by Indian Institute of Management – Bangalore faculties, at Indian Institute of Chemical Technology, Hyderabad.
3. Participation in “Effective Communication & Scientific Writing Skill Development Workshop” on 26th February – 3rd March 2009, by The Centre for Personal Transformation, at Indian Institute of Chemical Biology, Kolkata.
4. Participation in “Users’ Training Programme on CSIR E-Journals Consortium” on 21st August 2006 at Indian Institute of Chemical Biology, Kolkata.
5. Participation in Workshop on “Laboratory Safety on Biological, Chemical & Radiation Hazzards” in 2005 at Indian Institute of Chemical Biology, Kolkata.
6. Participation in Workshop on “Electron Microscopy For Beginners” in 2003 at Jadavpur University, Kolkata.

 

Memberships:

• Society of Biological Chemists, India – Life Member since 2002.
• Indian Science Congress Association – Life Member since 2004.

 

International Exposures for Research Purpose:

• London, United Kingdom – 2007.
• Chicago, USA – 2010, 2011.
• Bangkok, Thailand – 2010.
• Taipei, Taiwan – 2011, 2012, 2013, 2014, 2015.

 

Extra Curricular Activities:

• Training Consultant in British Council’s “Darwin Now” programme, 2010.
• Judge in the British Council’s School Debate 2008 entitled “Debating Matters, India”.
• Participated in various inter-organization competitive cricket tournaments of IICB and CSIR during 2002-2011 & 2015. Played for Royal Indian Cricket Club, Taiwan during 2012-2015.

 

Summary of Key Research Achievements:

 

Ph.D. Research Achievements:

• The presently available cell motility-analyzers measure primarily the ‘‘horizontal’’ velocity and there is no instrument available for ‘‘vertical’’ velocity measurement. Using goat sperm as the model a unique computer-based spectrophotometric system (SPERMA) has been developed for the first time to determine the average ‘‘vertical’’ velocity of motile cells. Undertaking upward movement against gravity is much tougher as compared with horizontal movement. Consequently average vertical velocity is expected to be a much better identifying parameter for assessing semen and other motile cell quality. The novel instrumental system developed in this research has thus the potential for immense application in human infertility clinics, animal-breeding centres, centres for conservation of endangered species, etc. (Cytometry Part A, 2007)

 

• Motility Stimulating Protein (MSP) is a heat stable 66 kDa monomeric novel protein purified from goat blood serum. At 0.9µM, it showed much higher forward motility and longer motility maintenance than other known activators. Motility analyzers CASA & SPERMA studies showed increase in horizontal and vertical velocities respectively. MSP action was cAMP independent. Its occurrence was higher in testis although blood was the richest source. MSP was localized throughout the cell surface of spermatozoa. Its antibody caused significant inhibition of sperm motility and inhibited fertilization showing its contraceptive efficacy. MSP has high efficacy to stimulate sperm forward motility thus may be used in biomedical application in infertility clinics, animal breeding centers, poultries, animal conservation centers. (Fertility & Sterility, 2013)

 

Post Doctoral Research Achievements:

 

• Information obtained from multiple sensory modalities, such as vision and touch, is integrated to yield a holistic percept. In this research a novel visual-tactile cross-modal integration stimulator has been developed with three degrees of freedom that can present tactile motion stimuli with arbitrary motion direction, speed, and indentation depth in the skin. The apparatus can present cross-modal stimuli in which the spatial locations of visual and tactile stimulations are perfectly aligned. Results showed that perceived direction of visual motion can be biased by the direction of tactile motion when visual signals are weakened. The results also showed that the visual-tactile motion integration follows the rule of temporal congruency of multi-modal inputs, a fundamental property known for cross-modal integration. The apparatus developed in the present study could be used for computational, psychophysical, and neurophysiological studies for research on rehabilitation of stroke patients. (Sensors, 2013)

 

• Cell cycle regulation plays a pivotal role in controlling cell proliferation and survival. Aberrant expression of cell cycle- associated genes generally afflicts cells with tumor-prone characteristics and promotes chromosome instability. FLJ10540 (Centrosome Protein 55kDa: Cep55) was identified recently as an important regulator of cytokinesis involved in cell growth and proliferation. Thus Cep55 may be considered for cancer regulation. Other than this testis is one of the tissues with most prominent Cep55 expression. Therefore, it must have some role related to testicular cell cycle progression. FLJ25439, a novel protein found in our laboratory (Cell Cycle, 2015), was also found to be expressed exclusively in testis and preliminarily found to be an interacting partner of Cep55. In this study, we generated Cep55 Knockout (KO) mice by microinjection of embryonic stem (ES) harboring Cep55 null allele into blastocysts and chimera mice produced were then mated with wild type (WT) C57BL/6 mice. Heterozygous (-/+) mice from different litters were then mated to finally get homozygous (-/-) mice which showed proportionate dwarfism with balancing problem and short life span. Thus, complete knockout of Cep55 may be detrimental to cell division (in vivo) and development of embryo.

@@@@@

CURRICULUM VITAE – Updated on 1/31/2019

Dr. Sudipta Saha

Assistant Professor (III),

Amity Institute of Physiology and Allied Sciences (AIPAS),

Amity University, Noida, Uttar Pradesh,

Sector-125, Noida – 201313

Mobile: +91 9830079019, +91 9810349961

E-Mail: sudiptasaha49@yahoo.co.in, sudiptasaha1977@gmail.com

Permanent Residence Address:

West Bengal, India.

 

An enthusiastic and skilled researcher in Reproductive Biology, Physiology, Cell Biology, Molecular Biology and Biochemistry.

Research Interest:

 Cell Biology, Molecular Biology and Physiology

 Reproductive Biology and Protein Biochemistry

 Bio-Instrumentation and Application Oriented Research

Professional Training:

 Assistant Professor (III), Amity Institute of Physiology and Allied Sciences, Amity University, Noida, UP.

June 2017 – Present

 Senior Research Associate (CSIR Scientists’ Pool Scheme), IICB, Kolkata, India.

November 2015 – June 2017

 Post Doctoral Research Associate (NSC), Chang Gung University, Taiwan.

November 2012 – April 2015

 Scientific Author, Curator & Reporter, http://www.pharmaceuticalintelligence.com,

July 2012 – Present (Associated without any payment)

 Post Doctoral Research Associate (NSC), Chang Gung Memorial Hospital, Taiwan.

June 2011 – June 2012

 Post Doctoral Research Associate (CSIR), Indian Institute of Chemical Biology (IICB), Kolkata, India.

February 2009 – June 2011

Research Techniques Known:

 Mammalian cell (U2OS, HEK293, 293T, 293FT, HeLa, C2C12, 3T3, LNCaP, PC3, etc. & different stable clones) and Bacterial cell culture, Primary cell culture, Mouse embryonic fibroblast culture, Baterial transformation, Plasmid purification, Transfection, Lentiviral transfection, PCR, experiments with gene knockout mice.

 ELISA, Western Blotting, Immunoprecipitation assay, immunofluorescence staining, fluorescent microscopy and FACS.

 Protein purification by different Chromatography, Electrophoresis, Histological slide preparation, etc.

 Animal handling: Laboratory rats, mice, rabbits and some surgery (ectomy), gene knockout mice breeding and experiments.

 Microscopic and spectrophotometric sperm motility assay techniques, General biochemical assays, etc.

 Process and instrument development by collaboration with different people with suitable expertise.

Education:

 Ph.D. in Life Science (Ph.D. awarded December 24, 2008)

CSIR-Indian Institute of Chemical Biology, Jadavpur University, Kolkata, India

Thesis Title: “A novel method of sperm motility analysis and characterisation of a sperm motility promoting protein from goat blood serum”. Advisor: Dr. G. C. Majumder and Dr. S. R. Dungdung, Sperm Biology Laboratory, Cell Biology and Physiology Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India. CV – Sudipta Saha Page 2

 Master of Science in Physiology (October 2001)

Presidency College, University of Calcutta, Kolkata, India. (Percentage of Marks: 64.10%)

Project Title: “Effect of oral exposure of arsenic on certain physiological parameters related to ovarian endocrine functions”. Advisor: Prof. Chandan Mitra, Head, Dept. of Physiology, Presidency College, Kolkata, India.

 Bachelor of Science in Physiology (August 1999)

City College, University of Calcutta, India. (Percentage of Marks: 56.75%, Honours Graduation)

With Minors: Zoology, Chemistry (Percentage of Marks: 60% in Pass Graduation)

 Madhyamick (10th, 1994, 79.44%) and Higher Secondary (10+2, 1996, 63.40%)

St. Lawrence High School, Kolkata, India.

Journal Papers:

1. Bhoumik A., Saha S., Payghan P. V., Ghosh P., Dungdung S. R. (2018): Localization of MIF-II on mammalian spermatozoa: A study revealing its structure, function and motility inhibitory pathway. International Journal of Biological Macromolecules, 116, 633-647.

2. Ghosh P., Bhoumik A., Saha S., Mukherjee S., Azmi S., Ghosh J. K., Dungdung S. R. (2017): Spermicidal efficacy of VRP, a synthetic cationic antimicrobial peptide, inducing apoptosis and membrane disruption. Journal of Cellular Physiology, 233(2), 1041-1050.

3. Pan T. L., Hsu S. Y., Wang P. W., Cheng Y. T., Chang Y. C., Saha S., Hu J., and Ouyang P. (2015): FLJ25439, a novel cytokinesis-associate protein, induces tetraploidization and maintains chromosomal stability via enhancing expression of endoplasmic reticulum stress chaeperones. Cell Cycle, 14(8), 1174-1187.

4. Saha S., Dungdung S. R., Majumder G. C., (2014): Determination of the antioxidant potential of goat sperm cells. Oxidants and Antioxidants in Medical Science, 3(3), 195-200.

5. Bhoumik A., Saha S., Majumder G. C., Dungdung S. R. (2014): Optimum calcium concentration: a crucial factor in regulating sperm motility in vitro. Cell Biochemistry and Biophysics, 70, 1177-1183.

6. Saha S., Das S., Bhoumik A., Ghosh P., Majumder G. C. and Dungdung S. R. (2013): Identification of a novel sperm motility stimulating protein from caprine serum: its characterization and functional significance. Fertility and Sterility, 100(1), 269-279.

(This paper was selected and listed by “Global Medical Discovery” as a potential bio-molecule for future drug development potential)

7. Pei Y.C., Chang T. Y., Lee T. C., Saha S., Lai H. Y., Gomez-Ramirez M., Chou S. W., Wong A. M. K. (2013): Cross-modal sensory integration of visual-tactile motion information: Instrument design and human psychophysics. Sensors, 13(6), 7212-7223.

8. Das S., Saha S., Majumder G. C. and Dungdung S. R. (2010): Purification and Characterization of a Sperm Motility Inhibiting Factor from Caprine Epididymal Plasma. PLoS ONE, 5(8): e12039 (1-12).

9. Jaiswal B. S., Das K., Saha S., Dungdung S. R., Majumder G. C. (2010): Purification and Characterization of a Motility Initiating Protein from Caprine Epididymal Plasma. Journal of Cellular Physiology, 222, 254-263.

10. Saha S., Paul D., Mukherjee A., Banerjee S., Majumder G. C. (2007): A computerized spectrophotometric instrumental system to determine the “vertical velocity” of sperm cells: a novel concept. Cytometry Part A. 71A, 308-316.

(“The Telegraph” reviewed this paper on 14 May, 2007; “News Online” reviewed this paper on Sept, 2009) (National[1605/DEL/2004], International [PCT/IB05/02541] patent applications filed, Won several awards including “Biotech Idea to Innovation Award 2007” from British Council; “India Innovation Pioneers Chalange 2009 from Indo-US Science and Technology Forum)

11. Mandal M., Saha S., Ghosh A. K., Majumder G. C. (2006): Identification and characterization of a sperm motility promoting glycoprotein from buffalo blood serum. Journal of Cellular Physiology, 209(2), 353-362.

(“The Statesman” reviewed this paper on 10 Jan, 2007; “Down to Earth” reviewed this paper on 15 Jan, 2007.)

12. Saha S., Paul D., Mukherjee A., Banerjee S., Majumder G. C. (2006): Development of a unique computerized spectrophotometric system with vertical movement of the cuvette for sperm motility analysis. In: Proceedings of Canadian Conference on Electrical and Computer Engineering (CCECE/CCGEI, IEEE – Ottawa, May 2006). pp. 183-186. Online Publication pp. 1399-1402, DOI: 10.1109/CCECE.2006.277432.

13. Chattopadhyay D., Dungdung S. R., Das K., Saha S., Mandal A. B., and Majumder G. C. (2005): Sperm motility inhibiting activity of a phytosterol from Alstonia macrophylla Wall ex A. DC. Leaf extract: a Tribal Medicine. Indian Journal of Experimental Biology, 43, 1104-1109.

14. Kundu C. N., Das K., Chakrabarty J., Dutta P., Bhattacharyya D., Saha S., Ghosh A., and Majumder G. C.

CV – Sudipta Saha Page 3

(2003): Development of a novel epididymal sperm model for evaluating cryoprotecting potential of various reagents. Indian Journal of Cryogenics, 28(3), 79-89.

Book Chapters / Review Articles:

1. Pearlman J. D., Bernstein L. H., Lev-Ari A., Saha S., Sag D., Dragoi D., Lal V. (2018): Pharmacological Agents in Treatment of Cardiovascular Diseases (Series A: Cardiovascular Diseases Book 5), Kindle Edition. (e-book) https://www.amazon.com/dp/B07MGSFDWR, Editors: Justine D. Pearlman, Larry H. Bernstein, Aviva Lev-Ari,, http://www.pharmaceuticalintelligence.com.

2. Bernstein L. H., Lev-Ari A., Williams S. J., Nallaseth F., Brook I., Saha S., Sag D., Saxena R., Barliya T., Thornton G. S. (2017): The VOICES of Patients, Hospitals CEOs, Health Care Providers, Caregivers and Families: Personal Experience with Critical Care and Invasive Medical Procedures, Kindle Edition. (e-book) https://www.amazon.com/dp/B076HGB6MZ, Editor: Gail S. Thornton, http://www.pharmaceuticalintelligence.com.

3. Bernstein L. H., Lev-Ari A., Williams S. J., Sag D., Robu I., Barliya T., Orchard-Webb D., Kaul A. F., Dragoi D., Saha S. (2017): The Immune System, Stress Signaling, Infectious Diseases and Therapeutic Implications, Kindle Edition. (e-book) https://www.amazon.com/dp/B075CXHY1B, Editor: Sudipta Saha, http://www.pharmaceuticalintelligence.com.

4. Dungdung S. R., Bhoumik, A., Saha S., Ghosh P., Das K., Mukherjee S., Nath D., Chakrabarty J., Kundu C., Jaiswal B. S., Mandal M., Maiti A., Roychowdhury M., Ray, D., Bhattacharyya D., Majumder G. C. (2016): Identification of Sperm Motility Regulatory Proteins: A Potential Approach towards Animal Reproduction. Review in Book: Insights from Animal Reproduction, Page: 161-177, Editor: Rita Payan Carreira, InTech, Rijeka, Croatia.

5. Pearlman J. D., Saxena R., Saha S., Sag D., Williams S. J., Baker M., Bernstein L. H., Lev-Ari A., Sonnenberg A. (2015): Regenerative and Translational Medicine: The Therapeutic Promise for Cardiovascular Diseases, Kindle Edition. (e-book) http://www.amazon.com/dp/B019UM909A, Editors: Larry H Bernstein, Aviva Lev-Ari, http://www.pharmaceuticalintelligence.com.

6. Pearlman J. D., Williams S. J., Barliya T., Saha S., Stoicescu M. Vatsa A., Lev-Ari A., Bernstein L. H., Sonnenberg A. (2015): Etiologies of Cardiovascular Diseases: Epigenetics, Genetics and Genomics, Kindle Edition. (e-book) http://www.amazon.com/dp/B018PNHJ84, Editors: Aviva Lev-Ari, Larry H Bernstein, http://www.pharmaceuticalintelligence.com.

7. Saha S., Saxena R., Barlyia T., Sarkar A., Feldman M. W., Sag D., Bernstein L. H., Williams S. J., Lev-Ari A., Sonnenberg A. (2015): Genomics Orientations for Personalized Medicine (Frontiers in Genomics Research Book 1) Kindle Edition. (e-book) http://www.amazon.com/dp/B018DHBUO6, Editors: Larry H Bernstein, Aviva Lev-Ari, Stephen J. Williams, http://www.pharmaceuticalintelligence.com.

8. Majumder G. C., Saha S., Das K., Nath D., Maiti A., Dey S., Roy D., Dey CS., Mitra S., Rana A., Chakrabarty J., Das S., Bhoumik A., Banerjee S., Mandal M., Jaiswal B. S., Ghosh P., Das A., Bhattacharyya D., and Dungdung S. R. (2015): Role of Sperm Surface Molecules in Motility Regulation.Review in Book: Mammalian Endocrinology and Male Reproductive Biology, Page: 197-244, Editor: Shiokumar Singh, CRC Press, Taylor & Francis Asia Pacific, New Delhi, India.

9. Bernstein L. H., Kandala P., Lev-Ari A., Saha S., Saxena R., Williams S., Sonnenberg A. (2015): Metabolic Genomics & Pharmaceutics: Volume I (BioMedicine – Metabolomics, Immunology, Infectious Diseases Book 1) Kindle Edition. (e-book) http://www.amazon.com/dp/B012BB0ZF0, Editor: Larry H Bernstein, http://www.pharmaceuticalintelligence.com.

10. Majumder G. C., , Das K., Saha S., Nath D., Maiti A., Das S., Dey N., Banerjee S., Barua M., Mandal M., Jaiswal B. S., Biswas R., Bhoumik A., Roy D., Dey S., Som J., Bhattacharyya D., Dungdung S. R. (2012): Purification and Characterization of Novel Sperm Motility-Related Proteins. (Review) Protein Purification, Page: 1-90 Editors: Miguel Benitez and Victoria Aguirre, Nova Science Publishers Inc., USA.

Patents:

1. D. Paul, G. C. Majumder, S. Saha, A. Mukherjee, S. Banerjee. A spectrophotometric system for measurement of vertical velocity of clinical and biological samples. Indian Patent Application Filed – No. 1605/DEL/2004, Date: 26.08.2004. Indian Patent Granted: Patent No. IN264556, Date of Grant: 06.01.2015, International Classification: G01N 21/25. International PCT Patent Application Filed – PCT/IB05/02541.

CV – Sudipta Saha Page 4

Press Mentions:

1. “Global Medical Discovery” listed our paper in 2013 on goat serum motility promoting protein as a potential bio-molecule for future drug discovery.

2. “News Online” reported our work on the sperm vertical velocity measuring instrumental system in their September 2009 issue on 14th September 2009 following “Indo-US Honorable Mention Award”.

3. “The Telegraph” has reviewed our work on the sperm vertical velocity measuring instrumental system in the “Know How” scientific section on May 14, 2007.

4. “Down to Earth” the renowned science and technology magazine has reviewed our work on buffalo serum motility promoting protein on January 15, 2007.

5. “The Statesman” has reviewed our work on buffalo serum motility promoting protein on January 10, 2007.

Awards & Honours:

1. Awarded Best Poster Award, Bangalore India Bio, 2011.

2. Awarded Honorable Mention Award in India Innovation Pioneers Challenge Business Plan Competition, Indo-US Science & Technology Forum, New Delhi, 2009.

3. Finalist in BioAsia Innovation Award, BioAsia, Hyderabad, 2009.

4. Finalist in Ranbaxy Young Scientist Award, Ranbaxy Research Laboratories, Gurgaon, 2008.

5. Awarded Biotech Idea to Innovation Award, British Council, New Delhi, 2007.

6. Awarded Best Poster Award, International Congress On Gamete Biology: Emerging Frontiers In Fertility And Contraceptive Development, National Institute of Immunology, New Delhi, 2006.

7. Awarded National Scholarship for getting Star Marks in Madhyamick Pariksha, National Scholarships Scheme, Govt. of West Bengal, 1994.

Workshops and Research Training:

1. Participation in the “Hands-on Basic Flow Cytometry Course” during 17 – 21 December 2009, by Centre for Cellular and Molecular Platform (C-CAMP) at National Centre for Biological Sciences (NCBS), Bangalore. (Training on Becton Dicson (BD) FACS Aria).

2. Participation in “CSIR 6th Technology Led Entrepreneurship Programme 2009” during 1 – 26 June 2009, by Indian Institute of Management – Bangalore faculties, at Indian Institute of Chemical Technology, Hyderabad.

3. Participation in “Effective Communication & Scientific Writing Skill Development Workshop” on 26th February – 3rd March 2009, by The Centre for Personal Transformation, at Indian Institute of Chemical Biology, Kolkata.

4. Participation in “Users’ Training Programme on CSIR E-Journals Consortium” on 21st August 2006 at Indian Institute of Chemical Biology, Kolkata.

5. Participation in Workshop on “Laboratory Safety on Biological, Chemical & Radiation Hazzards” in 2005 at Indian Institute of Chemical Biology, Kolkata.

6. Participation in Workshop on “Electron Microscopy For Beginners” in 2003 at Jadavpur University, Kolkata.

Memberships:

 Society of Biological Chemists, India – Life Member since 2002.

 Indian Science Congress Association – Life Member since 2004.

International Exposures for Research Purpose:

 London, United Kingdom – 2007.

 Chicago, USA – 2010, 2011.

 Bangkok, Thailand – 2010.

 Taipei, Taiwan – 2011, 2012, 2013, 2014, 2015.

CV – Sudipta Saha Page 5

Extra Curricular Activities:

 Training Consultant in British Council’s “Darwin Now” programme, 2010.

 Judge in the British Council’s School Debate 2008 entitled “Debating Matters, India”.

 Participated in various inter-organization competitive cricket tournaments of IICB and CSIR during 2002-2011 & 2015. Played for Royal Indian Cricket Club, Taiwan during 2012-2015.

Summary of Key Research Achievements:

Ph.D. Research Achievements:

 The presently available cell motility-analyzers measure primarily the ‘‘horizontal’’ velocity and there is no instrument available for ‘‘vertical’’ velocity measurement. Using goat sperm as the model a unique computer-based spectrophotometric system (SPERMA) has been developed for the first time to determine the average ‘‘vertical’’ velocity of motile cells. Undertaking upward movement against gravity is much tougher as compared with horizontal movement. Consequently average vertical velocity is expected to be a much better identifying parameter for assessing semen and other motile cell quality. The novel instrumental system developed in this research has thus the potential for immense application in human infertility clinics, animal-breeding centres, centres for conservation of endangered species, etc. (Cytometry Part A, 2007)

 Motility Stimulating Protein (MSP) is a heat stable 66 kDa monomeric novel protein purified from goat blood serum. At 0.9μM, it showed much higher forward motility and longer motility maintenance than other known activators. Motility analyzers CASA & SPERMA studies showed increase in horizontal and vertical velocities respectively. MSP action was cAMP independent. Its occurrence was higher in testis although blood was the richest source. MSP was localized throughout the cell surface of spermatozoa. Its antibody caused significant inhibition of sperm motility and inhibited fertilization showing its contraceptive efficacy. MSP has high efficacy to stimulate sperm forward motility thus may be used in biomedical application in infertility clinics, animal breeding centers, poultries, animal conservation centers. (Fertility & Sterility, 2013)

Post Doctoral Research Achievements:

 Information obtained from multiple sensory modalities, such as vision and touch, is integrated to yield a holistic percept. In this research a novel visual-tactile cross-modal integration stimulator has been developed with three degrees of freedom that can present tactile motion stimuli with arbitrary motion direction, speed, and indentation depth in the skin. The apparatus can present cross-modal stimuli in which the spatial locations of visual and tactile stimulations are perfectly aligned. Results showed that perceived direction of visual motion can be biased by the direction of tactile motion when visual signals are weakened. The results also showed that the visual-tactile motion integration follows the rule of temporal congruency of multi-modal inputs, a fundamental property known for cross-modal integration. The apparatus developed in the present study could be used for computational, psychophysical, and neurophysiological studies for research on rehabilitation of stroke patients. (Sensors, 2013)

 Cell cycle regulation plays a pivotal role in controlling cell proliferation and survival. Aberrant expression of cell cycle- associated genes generally afflicts cells with tumor-prone characteristics and promotes chromosome instability. FLJ10540 (Centrosome Protein 55kDa: Cep55) was identified recently as an important regulator of cytokinesis involved in cell growth and proliferation. Thus Cep55 may be considered for cancer regulation. Other than this testis is one of the tissues with most prominent Cep55 expression. Therefore, it must have some role related to testicular cell cycle progression. FLJ25439, a novel protein found in our laboratory (Cell Cycle, 2015), was also found to be expressed exclusively in testis and preliminarily found to be an interacting partner of Cep55. In this study, we generated Cep55 Knockout (KO) mice by microinjection of embryonic stem (ES) harboring Cep55 null allele into blastocysts and chimera mice produced were then mated with wild type (WT) C57BL/6 mice. Heterozygous (-/+) mice from different litters were then mated to finally get homozygous (-/-) mice which showed proportionate dwarfism with balancing problem and short life span. Thus, complete knockout of Cep55 may be detrimental to cell division (in vivo) and development of embryo.

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OLD VERSION

Dr. Sudipta Saha

Dr. Sudipta Saha, Ph. D.

CSIR-Senior Research Associate (Scientists’ Pool Officer)

Sperm Biology Laboratory, Room: 239,

Cell Biology and Physiology Division,

CSIR-Indian Institute of Chemical Biology,

4, Raja S. C. Mullick Road,

Jadavpur, Kolkata – 700 032,

West Bengal, India.

Phone:  +91 33 2499 5771 (Lab.)
Mobile: +91 98300 79019
E-Mail: 

sudiptasaha49@yahoo.co.in

sudiptasaha1977@gmail.com

sahasudipta@hotmail.com

 

 

 

An enthusiastic and skilled researcher in Reproductive Biology, Physiology, Cell Biology, Molecular Biology and Biochemistry looking for a suitable position.

An Indian Patent has been granted for the development of Sperm Vertical Velocity Analyzer (SPERMA).

The PCT / International Patent Application status is yet to be known.

Patent No. 264556 

Date of Grant: 06.01.2015

http://www.patestate.com/disp.jsp?patno=264556

Statement of Research and Career Interest

I did my masters degree in Physiology with specialization in Endocrinology and Reproductive Physiology from Presidency College, Kolkata. I continued my interest in the field of reproductive biology as a Ph. D. research scholar at the Indian Institute of Chemical Biology (a CSIR unit). During my Ph.D., I worked on sperm motility. Sperm motility is a determinant factor in the process of fertilization. We found serum of goat (also buffalo) contains an active factor that increases sperm motility to a much higher degree than the known motility promoters. The active molecule was purified from goat serum (also buffalo: Journal of Cellular Physiology., 2006, 209(2), 353-362) and characterized for its biochemical and physiological properties. During this biochemical study, I found that the limitation of measuring sperm velocity is restricted on the horizontal plane only. Unfortunately, vertical movement was always ignored, perhaps because of instrumental limitations. Movement against the gravity is always difficult and requires more energy.

According to our perception, vertical velocity may provide a better index for sperm fertility potential. This idea was put forward and with the collaboration of engineers and software professionals, we developed a spectrophotometer based computerized sperm vertical velocity measuring instrumental system. (Cytometry Part A., 2007, 71A, 308-316; Patents: PCT/IB05/02541 & 1605/DEL/2004).

After my Ph.D., I continued to work on the same field in Indian Institute of Chemical Biology as a Research Associate. Presently I am working as a Research Associate in Chang Gung Memorial Hospital, Taiwan on the topic “Effect of tactile stimulus on the somatosensory cortex of the brain, integration of visual and tactile stimulus and its application in the rehabilitation of stroke patients”.

I wish to pursue my career related to science directly or indirectly (research or non-research). I am hard working and highly motivated, and ready to take on new assignments in the areas of health care, biotechnology, science media & publications or science management utilizing 9 years of experience in leading scientific research. I am a Ph.D. in Biology (Science) and my doctorate was both much awarded (“Biotech Idea to Innovation Award” from the British Council, “Honourable Mention Award” from the Indo-US Science and Technology Forum, etc.), prolific (7 peer-reviewed international papers, 2 patents pending) and generated significant general interest (4 press mentions). I also got the chance to participate in Business entrepreneurship mentoring sessions at 6th CSIR Technology Led Entrepreneurship Programme 2009, IICT, Hyderabad and Indian School of Business (ISB), Hyderabad in 2009 related to my research. To me, biology research meant more than laboratory work; it developed my skills for innovation, interdisciplinary collaboration, international experience, presentation, teaching and mentoring, stakeholder communication, lateral thinking and overall discipline. I seek a position where I can apply all of the above experiences to enjoy my work and add value to my employer.

Research Interest:

• Reproductive Biology and Protein Biochemistry
• Cell Biology, Molecular Biology and Physiology
• Bio-Instrumentation and Application Oriented Research

Professional Training:

• Post Doctoral Research Associate (CSIR), Indian Institute of Chemical Biology, Kolkata, India.

2009, Feb – 2011, Jun

• Post Doctoral Research Associate (NSC), Chang Gung Memorial Hospital, Taiwan.                        2011, Jul – 2012, Jun
• Scientific Author, Curator & Reporter, http://www.pharmaceuticalintelligence.com,

2012, Jul – Present

• Post Doctoral Research Associate (NSC), Chang Gung University, Taiwan.

2012, Nov – 2015, Apr

Research Techniques Known:

• Mammalian cell (U2OS, HEK293, 293T, 293FT, HeLa, C2C12, 3T3, LNCaP, PC3, etc. & different stable clones) and Bacterial cell culture, Primary cell culture, Mouse embryonic fibroblast culture, Baterial transformation, Plasmid purification, Transfection, Lentiviral transfection, PCR, experiments with gene knockout mice.
• ELISA, Western Blotting, Immunoprecipitation assay, immunofluorescence staining, fluorescent microscopy and FACS.
• Protein purification by different Chromatography, Electrophoresis, Histological slide preparation, etc.
• Animal handling: Laboratory rats, mice, rabbits and some surgery (ectomy), gene knockout mice breeding and experiments.
• Microscopic and spectrophotometric sperm motility assay techniques, General biochemical assays, etc.
• Manuscript writing for paper publication, Preparing powerpoint slides and scientific lecture presentation.
• Process and instrument development by collaboration with different people with suitable expertise.
• System neuroscience experiments on human and nonhuman primates.

Education:

Ph.D. in Life Science (Ph.D. awarded December 24, 2008)

Jadavpur University, Kolkata, India

Thesis Title: “A novel method of sperm motility analysis and characterisation of a sperm motility promoting protein           from goat blood serum”. Advisor: Dr. G. C. Majumder and Dr. S. R. Dungdung, Sperm Biology Laboratory, Cell Biology and Physiology Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.

Master of Science in Physiology (October 2001)

Presidency College, University of Calcutta, Kolkata, India. (Percentage of Marks: 64.10%)

Project Title: “Effect of oral exposure of arsenic on certain physiological parameters related to ovarian endocrine functions”. Advisor: Prof. Chandan Mitra, Head, Dept. of Physiology, Presidency College, Kolkata, India.

Bachelor of Science in Physiology (August 1999)

City College, University of Calcutta, India. (Percentage of Marks: 56.75%, Honours Graduation)

With Minors: Zoology, Chemistry (Percentage of Marks: 60% in Pass Graduation)

Madhyamick (10^th, 1994, 79.44%) and Higher Secondary (10+2, 1996, 63.40%)

St. Lawrence High School, Kolkata, India.

 

Journal Papers:

1. Pan T. L., Hsu S. Y., Wang P. W., Cheng Y. T., Chang Y. C., Saha S., Hu J., and Ouyang P. (2015): FLJ25439, a novel cytokinesis-associate protein, induces tetraploidization and maintains chromosomal stability via enhancing expression of endoplasmic reticulum stress chaeperones. Cell Cycle, 14(8), 1174-1187.
2. Saha S., Dungdung S. R., Majumder G. C., (2014): Determination of the antioxidant potential of goat sperm cells. Oxidants and Antioxidants in Medical Science, 3(3), 195-200.
3. Bhoumik A., Saha S., Majumder G. C., Dungdung S. R. (2014): Optimum calcium concentration: a crucial factor in regulating sperm motility in vitro. Cell Biochemistry and Biophysics, 70, 1177-1183.
4. Saha S., Das S., Bhoumik A., Ghosh P., Majumder G. C. and Dungdung S. R. (2013): Identification of a novel sperm motility stimulating protein from caprine serum: its characterization and functional significance. Fertility and Sterility, 100(1), 269-279.

(This paper was selected and listed by “Global Medical Discovery” as a potential bio-molecule for future drug development potential)

5. Pei Y.C., Chang T. Y., Lee T. C., Saha S., Lai H. Y., Gomez-Ramirez M., Chou S. W., Wong A. M. K. (2013): Cross-modal sensory integration of visual-tactile motion information: Instrument design and human psychophysics. Sensors, 13(6), 7212-7223.
6. Das S., Saha S., Majumder G. C. and Dungdung S. R. (2010): Purification and Characterization of a Sperm Motility Inhibiting Factor from Caprine Epididymal Plasma. PLoS ONE, 5(8): e12039 (1-12).
7. Jaiswal B. S., Das K., Saha S., Dungdung S. R., Majumder G. C. (2010): Purification and Characterization of a Motility Initiating Protein from Caprine Epididymal Plasma. Journal of Cellular Physiology, 222, 254-263.
8. Saha S., Paul D., Mukherjee A., Banerjee S., Majumder G. C. (2007): A computerized spectrophotometric instrumental system to determine the “vertical velocity” of sperm cells: a novel concept. Cytometry Part A. 71A, 308-316.

(“The Telegraph” reviewed this paper on 14 May, 2007; “News Online” reviewed this paper on Sept, 2009) (National[1605/DEL/2004], International [PCT/IB05/02541] patent applications filed, Won several awards including “Biotech Idea to Innovation Award 2007” from British Council; “India Innovation Pioneers Chalange 2009 from Indo-US Science and Technology Forum)

9. Mandal M., Saha S., Ghosh A. K., Majumder G. C. (2006): Identification and characterization of a sperm motility promoting glycoprotein from buffalo blood serum. Journal of Cellular Physiology, 209(2), 353-362.

(“The Statesman” reviewed this paper on 10 Jan, 2007; “Down to Earth” reviewed this paper on 15 Jan, 2007.)

10. Saha S., Paul D., Mukherjee A., Banerjee S., Majumder G. C. (2006): Development of a unique computerized spectrophotometric system with vertical movement of the cuvette for sperm motility analysis. In: Proceedings of Canadian Conference on Electrical and Computer Engineering (CCECE/CCGEI, IEEE – Ottawa, May 2006). pp. 183-186. Online Publication pp. 1399-1402, DOI: 10.1109/CCECE.2006.277432.
11. Chattopadhyay D., Dungdung S. R., Das K., Saha S., Mandal A. B., and Majumder G. C. (2005): Sperm motility inhibiting activity of a phytosterol from Alstonia macrophylla Wall ex A. DC. Leaf extract: a Tribal Medicine. Indian Journal of Experimental Biology, 43, 1104-1109.
12. Kundu C. N., Das K., Chakrabarty J., Dutta P., Bhattacharyya D., Saha S., Ghosh A., and Majumder G. C. (2003): Development of a novel epididymal sperm model for evaluating cryoprotecting potential of various reagents. Indian Journal of Cryogenics, 28(3), 79-89.

Book Chapters / Review Articles:

 

1. Majumder G. C., , Das K., Saha S., Nath D., Maiti A., Das S., Dey N., Banerjee S., Barua M., Mandal M., Jaiswal B. S., Biswas R., Bhoumik A., Roy D., Dey S., Som J., Bhattacharyya D., Dungdung S. R. (2012): Purification and Characterization of Novel Sperm Motility-Related Proteins. (Review) Protein Purification, Page: 1-90 Editors: Miguel Benitez and Victoria Aguirre, Nova Science Publishers Inc., USA.
2. Bernstein L. H., Kandala P., Lev-Ari A., Saha S., Saxena R., Williams S., Sonnenberg A. (2015): Metabolic Genomics & Pharmaceutics: Volume I (BioMedicine – Metabolomics, Immunology, Infectious Diseases Book 1) Kindle Edition. (e-book) http://www.amazon.com/dp/B012BB0ZF0, Editor: Larry H Bernstein, http://www.pharmaceuticalintelligence.com.
3. Majumder G. C., Saha S., Das K., Nath D., Maiti A., Dey S., Roy D., Dey CS., Mitra S., Rana A., Chakrabarty J., Das S., Bhoumik A., Banerjee S., Mandal M., Jaiswal B. S., Ghosh P., Das A., Bhattacharyya D., and Dungdung S. R. (2015): Role of Sperm Surface Molecules in Motility Regulation.Review in Book: Mammalian Endocrinology and Male Reproductive Biology, Page: 197-244, Editor: Shiokumar Singh, CRC Press, Taylor & Francis Asia Pacific, New Delhi, India.
4. Saha S., Saxena R., Barlyia T., Sarkar A., Feldman M. W., Sag D., Bernstein L. H., Williams S. J., Lev-Ari A., Sonnenberg A. (2015): Genomics Orientations for Personalized Medicine (Frontiers in Genomics Research Book 1) Kindle Edition. (e-book) http://www.amazon.com/dp/B018DHBUO6, Editors: Larry H Bernstein, Aviva Lev-Ari, Stephen J. Williams, http://www.pharmaceuticalintelligence.com.
5. Pearlman J. D., Williams S. J., Barliya T., Saha S., Stoicescu M. Vatsa A., Lev-Ari A., Bernstein L. H., Sonnenberg A. (2015): Etiologies of Cardiovascular Diseases: Epigenetics, Genetics and Genomics, Kindle Edition. (e-book) http://www.amazon.com/dp/B018PNHJ84, Editors: Aviva Lev-Ari, Larry H Bernstein, http://www.pharmaceuticalintelligence.com.
6. Pearlman J. D., Saxena R., Saha S., Sag D., Williams S. J., Baker M., Bernstein L. H., Lev-Ari A., Sonnenberg A. (2015): Regenerative and Translational Medicine: The Therapeutic Promise for Cardiovascular Diseases, Kindle Edition. (e-book) http://www.amazon.com/dp/B019UM909A, Editors: Larry H Bernstein, Aviva Lev-Ari, http://www.pharmaceuticalintelligence.com.
7. Dungdung S. R., Bhoumik, A., Saha S., Ghosh P., Das K., Mukherjee S., Nath D., Chakrabarty J., Kundu C., Jaiswal B. S., Mandal M., Maiti A., Roychowdhury M., Ray, D., Bhattacharyya D., Majumder G. C. (2015): Identification of Sperm Motility Regulatory Proteins: A Potential Approach towards Animal Reproduction. Review in Book: Animal Reproduction, Editor: Rita Payan Carreira, InTech, Rijeka, Croatia. (In Press)

 

Patent Applications Filed:

 

1. Paul, G. C. Majumder,S. Saha, A. Mukherjee, S. Banerjee. Computer based spectrophotometric system to determine vertical velocity of clinical and biological samples. International PCT Patent Application Filed – PCT/IB05/02541.
2. Paul, G. C. Majumder,S. Saha, A. Mukherjee, S. Banerjee. A unique computer based spectrophotometric system with multiple height exposures of the cuvette to determine vertical velocity of spermatozoa for clinical and biological applications. Indian Patent Application Filed – No. 1605/DEL/2004.

 

Press Mentions:

1. “The Statesman” has reviewed our work on buffalo serum motility promoting protein on January 10, 2007.
2. “Down to Earth” the renowned science and technology magazine has reviewed our work on buffalo serum motility promoting protein on January 15, 2007.
3. “The Telegraph” has reviewed our work on the sperm vertical velocity measuring instrumental system in the “Know How” scientific section on May 14, 2007.
4. “News Online” reported our work on the sperm vertical velocity measuring instrumental system in their September 2009 issue on 14th September 2009 following “Indo-US Honorable Mention Award”.
5. “Global Medical Discovery” listed our paper in 2013 on goat serum motility promoting protein as a potential bio-molecule for future drug discovery.

Awards & Honours:

1. Awarded Best Poster Award, International Congress On Gamete Biology: Emerging Frontiers In Fertility And Contraceptive Development, National Institute of Immunology, New Delhi, 2006.
2. Awarded Biotech Idea to Innovation Award, British Council, New Delhi, 2007.
3. Awarded Honorable Mention Award in India Innovation Pioneers Challenge Business Plan Competition, Indo-US Science & Technology Forum, New Delhi, 2009.
4. Awarded Best Poster Award, Bangalore India Bio, 2011.
5. Finalist in BioAsia Innovation Award, BioAsia, Hyderabad, 2009.
6. Finalist in Ranbaxy Young Scientist Award, Ranbaxy Research Laboratories, Gurgaon, 2008.
7. Awarded National Scholarship for getting Star Marks in Madhyamick Pariksha, National Scholarships Scheme, Govt. of West Bengal, 1994.

Workshops and Research Training:

1. Participation in Workshop on “Electron Microscopy For Beginners” in 2003 at Jadavpur University, Kolkata.
2. Participation in Workshop on “Laboratory Safety on Biological, Chemical & Radiation Hazzards” in 2005 at Indian Institute of Chemical Biology, Kolkata.
3. Participation in “Users’ Training Programme on CSIR E-Journals Consortium” on 21st August 2006 at Indian Institute of Chemical Biology, Kolkata.
4. Participation in “Effective Communication & Scientific Writing Skill Development Workshop” on 26th February – 3rd March 2009, by The Centre for Personal Transformation, at Indian Institute of Chemical Biology, Kolkata.
5. Participation in “CSIR 6th Technology Led Entrepreneurship Programme 2009” during 1 – 26 June 2009, by Indian Institute of Management – Bangalore faculties, at Indian Institute of Chemical Technology, Hyderabad.
6. Participation in the “Hands-on Basic Flow Cytometry Course” during 17 – 21 December 2009, by Centre for Cellular and Molecular Platform (C-CAMP) at National Centre for Biological Sciences (NCBS), Bangalore. (Training on Becton Dicson (BD) FACS Aria).

Memberships:

• Society of Biological Chemists, India – Life Member since 2002.
• Indian Science Congress Association – Life Member since 2004.

International Exposures for Research Purpose:

• London, United Kingdom – 2007.
• Chicago, USA – 2010, 2011.
• Bangkok, Thailand – 2010.
• Taipei, Taiwan – 2011, 2012, 2013, 2014, 2015.

Extra Curricular Activities:

• Training Consultant in British Council’s “Darwin Now” programme, 2010.
• Judge in the British Council’s School Debate 2008 entitled “Debating Matters, India”.
• Participated in various inter-organization competitive cricket tournaments of IICB and CSIR during 2002-2011 & 2015.

References and further details may be provided on request

Summary of Key Research Achievements:

 

Ph.D. Research Achievements:

 

• The presently available cell motility-analyzers measure primarily the ‘‘horizontal’’ velocity and there is no instrument available for ‘‘vertical’’ velocity measurement. Using goat sperm as the model a unique computer-based spectrophotometric system (SPERMA) has been developed for the first time to determine the average ‘‘vertical’’ velocity of motile cells. Undertaking upward movement against gravity is much tougher as compared with horizontal movement. Consequently average vertical velocity is expected to be a much better identifying parameter for assessing semen and other motile cell quality. The novel instrumental system developed in this research has thus the potential for immense application in human infertility clinics, animal-breeding centres, centres for conservation of endangered species, etc. (Cytometry Part A, 2007)

 

• Motility Stimulating Protein (MSP) is a heat stable 66 kDa monomeric novel protein purified from goat blood serum. At 0.9µM, it showed much higher forward motility and longer motility maintenance than other known activators. Motility analyzers CASA & SPERMA studies showed increase in horizontal and vertical velocities respectively. MSP action was cAMP independent. Its occurrence was higher in testis although blood was the richest source. MSP was localized throughout the cell surface of spermatozoa. Its antibody caused significant inhibition of sperm motility and inhibited fertilization showing its contraceptive efficacy. MSP has high efficacy to stimulate sperm forward motility thus may be used in biomedical application in infertility clinics, animal breeding centers, poultries, animal conservation centers. (Fertility & Sterility, 2013)

 

Post Doctoral Research Achievements:

 

• Information obtained from multiple sensory modalities, such as vision and touch, is integrated to yield a holistic percept. In this research a novel visual-tactile cross-modal integration stimulator has been developed with three degrees of freedom that can present tactile motion stimuli with arbitrary motion direction, speed, and indentation depth in the skin. The apparatus can present cross-modal stimuli in which the spatial locations of visual and tactile stimulations are perfectly aligned. Results showed that perceived direction of visual motion can be biased by the direction of tactile motion when visual signals are weakened. The results also showed that the visual-tactile motion integration follows the rule of temporal congruency of multi-modal inputs, a fundamental property known for cross-modal integration. The apparatus developed in the present study could be used for computational, psychophysical, and neurophysiological studies for research on rehabilitation of stroke patients. (Sensors, 2013)

 

• Cell cycle regulation plays a pivotal role in controlling cell proliferation and survival. Aberrant expression of cell cycle- associated genes generally afflicts cells with tumor-prone characteristics and promotes chromosome instability. FLJ10540 (Centrosome Protein 55kDa: Cep55) was identified recently as an important regulator of cytokinesis involved in cell growth and proliferation. Thus Cep55 may be considered for cancer regulation. Other than this testis is one of the tissues with most prominent Cep55 expression. Therefore, it must have some role related to testicular cell cycle progression. FLJ25439, a novel protein found in our laboratory (Cell Cycle, 2015), was also found to be expressed exclusively in testis and preliminarily found to be an interacting partner of Cep55. In this study, we generated Cep55 Knockout (KO) mice by microinjection of embryonic stem (ES) harboring Cep55 null allele into blastocysts and chimera mice produced were then mated with wild type (WT) C57BL/6 mice. Heterozygous (-/+) mice from different litters were then mated to finally get homozygous (-/-) mice which showed proportionate dwarfism with balancing problem and short life span. Thus, complete knockout of Cep55 may be detrimental to cell division (in vivo) and development of embryo.