Veins Construction using Autologous Peripheral Whole Blood
Reporter: Aviva Lev-Ari, PhD, RN
In vivo application of tissue-engineered veins using autologous peripheral whole blood: A proof of concept study
- a Department of Surgery, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, Sweden
- b SP Technical Research Institute of Sweden, Dept. of Chemistry, Materials and Surfaces, SE-50462 Borås, Sweden
- c Department of Paediatrics, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, Sweden
- d Department of Cardiothoracic Surgery, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, Sweden
- Received 2 September 2014, Revised 16 September 2014, Accepted 16 September 2014, Available online 22 September 2014
Michael Olausson, Vijay Kumar Kuna, Galyna Travnikova, Henrik Bäckdahl, Pradeep B. Patil, Robert Saalman, Helena Borg, Anders Jeppsson, Suchitra Sumitran-Holgersson. In vivo application of tissue-engineered veins using autologous peripheral whole blood: The proof of concept study. EBioMedicine, 2014; DOI: 10.1016/j. ebiom. 2014.09.001
Read more at: http://ciencia.me/tecnica-vaso-sanguineo-uma-semana/?lang=en
Highlights
- • Our study is a proof-of-concept clinical report of the successful recellularisation of two decellularised human blood vessels with autologous whole peripheral blood, which were subsequently used for a bypass procedure in two patients with portal vein thrombosis without the need for immunosuppression.
- • The work is important conceptually because it provides early evidence for generating clinically useful personalized blood vessels using a simple blood sample from the patient.
- • The work also establishes the feasibility and safety of a novel paradigm for treatment, in cases of venous insufficiency, obstructed veins or inadequate autologous veins within a short period of time.
Abstract
Vascular diseases are increasing health problems affecting > 25 million individuals in westernized societies. Such patients could benefit from transplantation of tissue-engineered vascular grafts using autologous cells. One challenge that has limited this development is the need for cell isolation, and risks associated with ex vivo expanded stem cells. Here we demonstrate a novel approach to generate transplantable vascular grafts using decellularized allogeneic vascular scaffolds, repopulated with peripheral whole blood (PWB) in vitro in a bioreactor. Circulating, VEGFR-2 + /CD45 + and a smaller fraction of VEGFR-2 +/CD14 + cells contributed to repopulation of the graft. SEM micrographs showed flat cells on the luminal surface of the grafts consistent with endothelial cells. For clinical validation, two autologous PWB tissue-engineered vein conduits were prepared and successfully used for by-pass procedures in two pediatric patients. These results provide a proof of principle for the generation of transplantable vascular grafts using a simple autologous blood sample, making it clinically feasible globally.
Keywords
- Vein conduits;
- vascular diseases;
- tissue-engineering;
- endothelial precursors
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