Leaders in Pharmaceutical Business Intelligence (LPBI) Group

Post-Translational Modifications

Author and Curator: Larry H Bernstein, MD, FCAP 

Modification-specific proteomics: characterization of post-translational modifications by mass spectrometry.

Jensen ON.
Curr Opin Chem Biol. 2004 Feb; 8(1):33-41.   PMID: 15036154

Post-translational modifications generate tremendous

• diversity,
• complexity and
• heterogeneity of gene products, and

their determination is one of the main challenges in proteomics research.

Recent developments in mass spectrometry based approaches for systematic, qualitative and quantitative determination of modified proteins promise to bring new insights on the

• dynamics and
• spatio-temporal control of protein activities
  • by post-translational modifications, and
  • reveal their roles in biological processes and pathogenic conditions.

Combinations of

1. affinity-based enrichment and extraction methods,
2. multidimensional separation technologies and
3. mass spectrometry

are particularly attractive for systematic investigation of post-translationally modified proteins in proteomics.

PTM modifications