In-vitro three-dimensional interaction between opposite cells
Author Danut Dragoi, PhD
Introduction
It is a trend of abnormal cells to have pectin on surface of the cell, see link in here for pectin structure as tentacles and attach to any cell in its way, see link in here and in here and in here.
An artificial three-dimensional (3D) culture system that mimics the tumor micro environment in vitro is an essential tool for investigating the cross-talk between immune and cancer cells in tumors, see link in here.
The development of 3D culture system using an electrospun poly(ε-caprolactone) (PCL) nanofibrous scaffold (NFS) and imaging are the two key elements to investigate pre-evolution of cancer cells. A hybrid NFS containing an uninterrupted network of nano- and sub-micron-scale fibers (400 nm to 2 µm) was generated by deposition onto a stainless steel mesh instead of an aluminum plate. The hybrid NFS contained multiplanar pores in a 3D structure. Surface-seeded mouse CT26 colon cancer cells and bone marrow-derived dendritic cells (BM-DCs) were able to infiltrate the hybrid NFS within several hours. BM-DCs cultured on PCL nanofibers showed a baseline inactive form, and lipopolysaccharide (LPS)-activated BM-DCs showed increased expression of CD86 and major histocompatibility complex Class II. Actin, see link here and phosphorylated FAK (Focal Adhesion Kinase, see link here and for kinase definition see link in here) were enriched where unstimulated and LPS-stimulated BM-DCs contacted the fibers in the 3D hybrid NFS. When BM-DCs were cocultured with mitoxantrone-treated CT26 cells in a 3D hybrid NFS, BM-DCs sprouted cytoplasm to, migrated to, synapsed with, and engulfed mitoxantrone-treated CT26 cancer cells, which were similar to the naturally occurring cross-talk between these two types of cells. The 3D hybrid NFS developed here provides a 3D structure for coculture of cancer and immune cells.
The relative random walk of CT26b cells can be seen on the link for watch video. The Stainless Steel substrate that has some rem-anent magnetism suggests a magnetic domain boundary effect on moving cells.At magnetic domain limit boundaries, the magnetic field gradient can move non-magnetic materials towards the highest gradient. Perhaps in the movie, this is the reason for seeing relative random walks of the cells.
The picture below shows the size and time scale of the cells moving around to find the minimum energy and eventually coalesce with the other cells building a multidimensional cell structure.
Watch video
Image adapted from: https://www.dovepress.com/articles.php?article_id=25822
The coalescence between cells visible in the video and the picture above suggests a specific interaction through the pectin on surface of every cell, which is a very sticky material as any sugar molecule do that. Destroying these pectin bounds is the key of stopping cancer cells to spread around. Perhaps destroying the source of pectin, which we believe is in the sugar consumption, will have a positive impact on fighting against the ultimate disease.
Source
http://pharmaceuticalintelligence.com/2016/02/23/a-thermodynamic-modeling-for-cancer-cells/
https://www.dovepress.com/articles.php?article_id=25822
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